CA3236802A1 - Serine recombinases - Google Patents
Serine recombinases Download PDFInfo
- Publication number
- CA3236802A1 CA3236802A1 CA3236802A CA3236802A CA3236802A1 CA 3236802 A1 CA3236802 A1 CA 3236802A1 CA 3236802 A CA3236802 A CA 3236802A CA 3236802 A CA3236802 A CA 3236802A CA 3236802 A1 CA3236802 A1 CA 3236802A1
- Authority
- CA
- Canada
- Prior art keywords
- sequence
- cell
- amino acid
- recombinase
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/22—Endodeoxyribonucleases producing 3'-phosphomonoesters (3.1.22)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163275288P | 2021-11-03 | 2021-11-03 | |
| US63/275,288 | 2021-11-03 | ||
| US202263322712P | 2022-03-23 | 2022-03-23 | |
| US63/322,712 | 2022-03-23 | ||
| US202263400868P | 2022-08-25 | 2022-08-25 | |
| US63/400,868 | 2022-08-25 | ||
| PCT/US2022/079227 WO2023081762A2 (en) | 2021-11-03 | 2022-11-03 | Serine recombinases |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3236802A1 true CA3236802A1 (en) | 2023-05-11 |
Family
ID=86242181
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3236802A Pending CA3236802A1 (en) | 2021-11-03 | 2022-11-03 | Serine recombinases |
Country Status (7)
| Country | Link |
|---|---|
| US (1) | US20240417754A1 (https=) |
| EP (1) | EP4427229A4 (https=) |
| JP (1) | JP2024543042A (https=) |
| KR (1) | KR20240099418A (https=) |
| AU (1) | AU2022381188A1 (https=) |
| CA (1) | CA3236802A1 (https=) |
| WO (1) | WO2023081762A2 (https=) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2025537169A (ja) * | 2022-11-01 | 2025-11-14 | アーク リサーチ インスティテュート | リコンビナーゼ融合体 |
| CN116479035B (zh) * | 2023-03-30 | 2024-04-26 | 西北农林科技大学 | 一种定点突变创制草莓耐弱光种质的方法及其应用 |
| WO2025160202A1 (en) * | 2024-01-22 | 2025-07-31 | Arc Researrch Institute | Engineered large serine recombinases |
| WO2025193723A1 (en) | 2024-03-12 | 2025-09-18 | Stylus Medicine, Inc. | Non-viral circular single-stranded dna systems and uses thereof |
| EP4703469A1 (en) | 2024-08-26 | 2026-03-04 | Charité - Universitätsmedizin Berlin Körperschaft des öffentlichen Rechts | Fusion proteins, methods and compositions for efficient serine integrase-mediated gene transfer in human cells |
| WO2026046974A1 (en) | 2024-08-26 | 2026-03-05 | Charité - Universitätsmedizin Berlin Körperschaft des öffentlichen Rechts | Fusion proteins, methods and compositions for efficient serine integrase-mediated gene transfer in human cells |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105969807A (zh) * | 2011-04-05 | 2016-09-28 | 斯克利普斯研究所 | 染色体着陆垫及相关用途 |
| EP2840140B2 (en) * | 2012-12-12 | 2023-02-22 | The Broad Institute, Inc. | Crispr-Cas based method for mutation of prokaryotic cells |
| US10731153B2 (en) * | 2016-01-21 | 2020-08-04 | Massachusetts Institute Of Technology | Recombinases and target sequences |
| CA3162499A1 (en) * | 2019-11-22 | 2021-05-27 | Flagship Pioneering Innovations Vi, Llc | Recombinase compositions and methods of use |
| WO2021119225A1 (en) * | 2019-12-10 | 2021-06-17 | Homodeus, Inc. | Recombinase discovery |
-
2022
- 2022-11-03 WO PCT/US2022/079227 patent/WO2023081762A2/en not_active Ceased
- 2022-11-03 CA CA3236802A patent/CA3236802A1/en active Pending
- 2022-11-03 KR KR1020247018577A patent/KR20240099418A/ko active Pending
- 2022-11-03 JP JP2024526843A patent/JP2024543042A/ja active Pending
- 2022-11-03 EP EP22891045.1A patent/EP4427229A4/en active Pending
- 2022-11-03 US US18/706,301 patent/US20240417754A1/en active Pending
- 2022-11-03 AU AU2022381188A patent/AU2022381188A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2024543042A (ja) | 2024-11-19 |
| EP4427229A2 (en) | 2024-09-11 |
| KR20240099418A (ko) | 2024-06-28 |
| WO2023081762A2 (en) | 2023-05-11 |
| AU2022381188A1 (en) | 2024-05-23 |
| WO2023081762A3 (en) | 2023-06-15 |
| EP4427229A4 (en) | 2025-11-12 |
| US20240417754A1 (en) | 2024-12-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| P13 | Application amended |
Free format text: ST27 STATUS EVENT CODE: A-1-1-P10-P13-P146 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: BSL VERIFIED - NO DEFECTS Effective date: 20241017 |
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| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 2ND ANNIV.) - STANDARD Year of fee payment: 2 |
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| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20241025 |
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| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT Effective date: 20241025 Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20241025 |
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| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 3RD ANNIV.) - STANDARD Year of fee payment: 3 |
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| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20251024 |
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| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-1-1-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20251024 |