JP2024543042A - セリンリコンビナーゼ - Google Patents

セリンリコンビナーゼ Download PDF

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Publication number
JP2024543042A
JP2024543042A JP2024526843A JP2024526843A JP2024543042A JP 2024543042 A JP2024543042 A JP 2024543042A JP 2024526843 A JP2024526843 A JP 2024526843A JP 2024526843 A JP2024526843 A JP 2024526843A JP 2024543042 A JP2024543042 A JP 2024543042A
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JP
Japan
Prior art keywords
sequence
amino acid
cell
recombinase
cells
Prior art date
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Pending
Application number
JP2024526843A
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English (en)
Japanese (ja)
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JP2024543042A5 (https=
Inventor
アミ エス バット
マシュー ジー デュラント
ジョシュア シー タイコ
パトリック ディー シュー
アリソン ファントン
マイケル シー バシク
ラクラミオアラ ビントゥ
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California San Diego UCSD
Original Assignee
University of California San Diego UCSD
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California San Diego UCSD filed Critical University of California San Diego UCSD
Publication of JP2024543042A publication Critical patent/JP2024543042A/ja
Publication of JP2024543042A5 publication Critical patent/JP2024543042A5/ja
Pending legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/22—Endodeoxyribonucleases producing 3'-phosphomonoesters (3.1.22)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2024526843A 2021-11-03 2022-11-03 セリンリコンビナーゼ Pending JP2024543042A (ja)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202163275288P 2021-11-03 2021-11-03
US63/275,288 2021-11-03
US202263322712P 2022-03-23 2022-03-23
US63/322,712 2022-03-23
US202263400868P 2022-08-25 2022-08-25
US63/400,868 2022-08-25
PCT/US2022/079227 WO2023081762A2 (en) 2021-11-03 2022-11-03 Serine recombinases

Publications (2)

Publication Number Publication Date
JP2024543042A true JP2024543042A (ja) 2024-11-19
JP2024543042A5 JP2024543042A5 (https=) 2026-03-25

Family

ID=86242181

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2024526843A Pending JP2024543042A (ja) 2021-11-03 2022-11-03 セリンリコンビナーゼ

Country Status (7)

Country Link
US (1) US20240417754A1 (https=)
EP (1) EP4427229A4 (https=)
JP (1) JP2024543042A (https=)
KR (1) KR20240099418A (https=)
AU (1) AU2022381188A1 (https=)
CA (1) CA3236802A1 (https=)
WO (1) WO2023081762A2 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2025537169A (ja) * 2022-11-01 2025-11-14 アーク リサーチ インスティテュート リコンビナーゼ融合体
CN116479035B (zh) * 2023-03-30 2024-04-26 西北农林科技大学 一种定点突变创制草莓耐弱光种质的方法及其应用
WO2025160202A1 (en) * 2024-01-22 2025-07-31 Arc Researrch Institute Engineered large serine recombinases
WO2025193723A1 (en) 2024-03-12 2025-09-18 Stylus Medicine, Inc. Non-viral circular single-stranded dna systems and uses thereof
EP4703469A1 (en) 2024-08-26 2026-03-04 Charité - Universitätsmedizin Berlin Körperschaft des öffentlichen Rechts Fusion proteins, methods and compositions for efficient serine integrase-mediated gene transfer in human cells
WO2026046974A1 (en) 2024-08-26 2026-03-05 Charité - Universitätsmedizin Berlin Körperschaft des öffentlichen Rechts Fusion proteins, methods and compositions for efficient serine integrase-mediated gene transfer in human cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105969807A (zh) * 2011-04-05 2016-09-28 斯克利普斯研究所 染色体着陆垫及相关用途
EP2840140B2 (en) * 2012-12-12 2023-02-22 The Broad Institute, Inc. Crispr-Cas based method for mutation of prokaryotic cells
US10731153B2 (en) * 2016-01-21 2020-08-04 Massachusetts Institute Of Technology Recombinases and target sequences
CA3162499A1 (en) * 2019-11-22 2021-05-27 Flagship Pioneering Innovations Vi, Llc Recombinase compositions and methods of use
WO2021119225A1 (en) * 2019-12-10 2021-06-17 Homodeus, Inc. Recombinase discovery

Also Published As

Publication number Publication date
EP4427229A2 (en) 2024-09-11
KR20240099418A (ko) 2024-06-28
WO2023081762A2 (en) 2023-05-11
AU2022381188A1 (en) 2024-05-23
WO2023081762A3 (en) 2023-06-15
EP4427229A4 (en) 2025-11-12
US20240417754A1 (en) 2024-12-19
CA3236802A1 (en) 2023-05-11

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