KR20240099418A - 세린 재조합효소 - Google Patents

세린 재조합효소 Download PDF

Info

Publication number
KR20240099418A
KR20240099418A KR1020247018577A KR20247018577A KR20240099418A KR 20240099418 A KR20240099418 A KR 20240099418A KR 1020247018577 A KR1020247018577 A KR 1020247018577A KR 20247018577 A KR20247018577 A KR 20247018577A KR 20240099418 A KR20240099418 A KR 20240099418A
Authority
KR
South Korea
Prior art keywords
sequence
amino acid
cells
recombinase
cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
KR1020247018577A
Other languages
English (en)
Korean (ko)
Inventor
아미 에스. 바트
매튜 지. 더런트
조슈아 씨. 타이코
패트릭 디. 수
앨리슨 팬톤
마이클 씨. 바식
라크라미오아라 빈투
Original Assignee
더 리전트 오브 더 유니버시티 오브 캘리포니아
솔크 인스티튜트 포 바이올로지칼 스터디즈
더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티
더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 더 리전트 오브 더 유니버시티 오브 캘리포니아, 솔크 인스티튜트 포 바이올로지칼 스터디즈, 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티, 더 보드 어브 트러스티스 어브 더 리랜드 스탠포드 주니어 유니버시티 filed Critical 더 리전트 오브 더 유니버시티 오브 캘리포니아
Publication of KR20240099418A publication Critical patent/KR20240099418A/ko
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/22—Endodeoxyribonucleases producing 3'-phosphomonoesters (3.1.22)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
KR1020247018577A 2021-11-03 2022-11-03 세린 재조합효소 Pending KR20240099418A (ko)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US202163275288P 2021-11-03 2021-11-03
US63/275,288 2021-11-03
US202263322712P 2022-03-23 2022-03-23
US63/322,712 2022-03-23
US202263400868P 2022-08-25 2022-08-25
US63/400,868 2022-08-25
PCT/US2022/079227 WO2023081762A2 (en) 2021-11-03 2022-11-03 Serine recombinases

Publications (1)

Publication Number Publication Date
KR20240099418A true KR20240099418A (ko) 2024-06-28

Family

ID=86242181

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020247018577A Pending KR20240099418A (ko) 2021-11-03 2022-11-03 세린 재조합효소

Country Status (7)

Country Link
US (1) US20240417754A1 (https=)
EP (1) EP4427229A4 (https=)
JP (1) JP2024543042A (https=)
KR (1) KR20240099418A (https=)
AU (1) AU2022381188A1 (https=)
CA (1) CA3236802A1 (https=)
WO (1) WO2023081762A2 (https=)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2025537169A (ja) * 2022-11-01 2025-11-14 アーク リサーチ インスティテュート リコンビナーゼ融合体
CN116479035B (zh) * 2023-03-30 2024-04-26 西北农林科技大学 一种定点突变创制草莓耐弱光种质的方法及其应用
WO2025160202A1 (en) * 2024-01-22 2025-07-31 Arc Researrch Institute Engineered large serine recombinases
WO2025193723A1 (en) 2024-03-12 2025-09-18 Stylus Medicine, Inc. Non-viral circular single-stranded dna systems and uses thereof
EP4703469A1 (en) 2024-08-26 2026-03-04 Charité - Universitätsmedizin Berlin Körperschaft des öffentlichen Rechts Fusion proteins, methods and compositions for efficient serine integrase-mediated gene transfer in human cells
WO2026046974A1 (en) 2024-08-26 2026-03-05 Charité - Universitätsmedizin Berlin Körperschaft des öffentlichen Rechts Fusion proteins, methods and compositions for efficient serine integrase-mediated gene transfer in human cells

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN105969807A (zh) * 2011-04-05 2016-09-28 斯克利普斯研究所 染色体着陆垫及相关用途
EP2840140B2 (en) * 2012-12-12 2023-02-22 The Broad Institute, Inc. Crispr-Cas based method for mutation of prokaryotic cells
US10731153B2 (en) * 2016-01-21 2020-08-04 Massachusetts Institute Of Technology Recombinases and target sequences
CA3162499A1 (en) * 2019-11-22 2021-05-27 Flagship Pioneering Innovations Vi, Llc Recombinase compositions and methods of use
WO2021119225A1 (en) * 2019-12-10 2021-06-17 Homodeus, Inc. Recombinase discovery

Also Published As

Publication number Publication date
JP2024543042A (ja) 2024-11-19
EP4427229A2 (en) 2024-09-11
WO2023081762A2 (en) 2023-05-11
AU2022381188A1 (en) 2024-05-23
WO2023081762A3 (en) 2023-06-15
EP4427229A4 (en) 2025-11-12
US20240417754A1 (en) 2024-12-19
CA3236802A1 (en) 2023-05-11

Similar Documents

Publication Publication Date Title
US20240417754A1 (en) Serine recombinases
US20220033858A1 (en) Crispr oligoncleotides and gene editing
US20220195403A1 (en) Methods of achieving high specificity of genome editing
KR20220004674A (ko) Rna를 편집하기 위한 방법 및 조성물
US20170204407A1 (en) Crispr/cas transcriptional modulation
JP2018532419A (ja) ＣＲＩＳＰＲ−Ｃａｓ ｓｇＲＮＡライブラリー
US20240279629A1 (en) Crispr-transposon systems for dna modification
US11834652B2 (en) Compositions and methods for scarless genome editing
CN110343724A (zh) 用于筛选和鉴定功能性lncRNA的方法
US20250376701A1 (en) Crispr-transposon systems and components
US20190218533A1 (en) Genome-Scale Engineering of Cells with Single Nucleotide Precision
US11608570B2 (en) Targeted in situ protein diversification by site directed DNA cleavage and repair
JP2024501892A (ja) 新規の核酸誘導型ヌクレアーゼ
KR102667508B1 (ko) 프라임 에디팅 시스템을 이용한 게놈 편집의 과정에서 발생 가능한 오프 타겟을 예측하는 방법
CN111334531A (zh) 高信噪比阴性遗传筛选方法
WO2025071410A1 (en) System for deleting regions of dna
WO2025160202A1 (en) Engineered large serine recombinases
WO2023070043A1 (en) Compositions and methods for targeted editing and evolution of repetitive genetic elements
CN117795085A (zh) 用于dna修饰的crispr-转座子系统
WO2026038057A1 (en) Improved methods for gene editing
CN118165956A (zh) 基于Tsp2Cas9蛋白的CRISPR/Cas9基因编辑系统及其相关应用
Weingarten-Gabbay Unraveling the regulatory code of transcription and translation in the human genome and viruses

Legal Events

Date Code Title Description
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

E13 Pre-grant limitation requested

Free format text: ST27 STATUS EVENT CODE: A-2-3-E10-E13-LIM-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-NAP-X000 (AS PROVIDED BY THE NATIONAL OFFICE)

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000