CA3223009A1 - Systems, methods, and compositions comprising miniature crispr nucleases for gene editing and programmable gene activation and inhibition - Google Patents

Systems, methods, and compositions comprising miniature crispr nucleases for gene editing and programmable gene activation and inhibition Download PDF

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Publication number
CA3223009A1
CA3223009A1 CA3223009A CA3223009A CA3223009A1 CA 3223009 A1 CA3223009 A1 CA 3223009A1 CA 3223009 A CA3223009 A CA 3223009A CA 3223009 A CA3223009 A CA 3223009A CA 3223009 A1 CA3223009 A1 CA 3223009A1
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Canada
Prior art keywords
acid sequence
seq
target specific
specific nuclease
composition
Prior art date
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Pending
Application number
CA3223009A
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English (en)
French (fr)
Inventor
Kaiyi JIANG
Lukas VILLIGER
Omar Osama Abudayyeh
Jonathan S. Gootenberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Massachusetts Institute of Technology
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Massachusetts Institute of Technology
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by Massachusetts Institute of Technology filed Critical Massachusetts Institute of Technology
Publication of CA3223009A1 publication Critical patent/CA3223009A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3223009A 2021-06-17 2022-06-16 Systems, methods, and compositions comprising miniature crispr nucleases for gene editing and programmable gene activation and inhibition Pending CA3223009A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163211610P 2021-06-17 2021-06-17
US63/211,610 2021-06-17
PCT/US2022/033749 WO2022266298A1 (en) 2021-06-17 2022-06-16 Systems, methods, and compositions comprising miniature crispr nucleases for gene editing and programmable gene activation and inhibition

Publications (1)

Publication Number Publication Date
CA3223009A1 true CA3223009A1 (en) 2022-12-22

Family

ID=82404474

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3223009A Pending CA3223009A1 (en) 2021-06-17 2022-06-16 Systems, methods, and compositions comprising miniature crispr nucleases for gene editing and programmable gene activation and inhibition

Country Status (6)

Country Link
US (1) US20240309348A1 (https=)
EP (1) EP4355869A1 (https=)
JP (1) JP2024522764A (https=)
AU (1) AU2022292659A1 (https=)
CA (1) CA3223009A1 (https=)
WO (1) WO2022266298A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116376874B (zh) * 2023-03-24 2025-07-22 尧唐(上海)生物科技有限公司 一种Cas蛋白、其基因编辑系统及应用
WO2024249770A2 (en) * 2023-06-01 2024-12-05 University Of Southern California Spatiotemporal control of genomics and epigenomics by ultrasound
AU2024357941A1 (en) * 2023-10-09 2026-04-02 Pairwise Plants Services, Inc. Circular permutants, compositions comprising the same, and methods of use thereof
WO2026019466A1 (en) * 2024-07-17 2026-01-22 Massachusetts Institute Of Technology Engineered t7 rna polymerases for improved rna production

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4554A (en) 1846-05-30 Island
US101A (en) 1836-12-06 Method of jcakibtg and furling iw sails fob ships
KR102929494B1 (ko) * 2018-10-29 2026-02-20 차이나 어그리컬처럴 유니버시티 신규 CRISPR/Cas12f 효소 및 시스템
WO2020123887A2 (en) * 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing
BR112021020770A2 (pt) * 2019-04-18 2022-01-04 Pioneer Hi Bred Int Fatores de embriogênese para reprogramação celular de uma célula vegetal
CN114846146B (zh) * 2019-10-29 2024-04-12 基恩科雷有限责任公司 用于增加CRISPR/Cas12f1系统的效率的工程化引导RNA及其用途
CA3192654A1 (en) * 2020-09-01 2022-03-10 The Board Of Trustees Of The Leland Stanford Junior Unversity Synthetic miniature crispr-cas (casmini) system for eukaryotic genome engineering
EP4227411A4 (en) * 2020-10-08 2025-07-09 Genkore Inc MODIFIED GUIDE RNA TO INCREASE THE EFFICIENCY OF THE CRISPR/CAS12F1 SYSTEM, AND ITS USE
WO2022075808A1 (ko) * 2020-10-08 2022-04-14 주식회사 진코어 Crispr/cas12f1 시스템 효율화를 위한 u-rich tail을 포함하는 엔지니어링 된 가이드 rna 및 그 용도

Also Published As

Publication number Publication date
AU2022292659A1 (en) 2023-12-21
US20240309348A1 (en) 2024-09-19
WO2022266298A1 (en) 2022-12-22
JP2024522764A (ja) 2024-06-21
EP4355869A1 (en) 2024-04-24

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