JP2024522764A - 遺伝子編集のため、並びにプログラム可能な遺伝子活性化及び阻害のための微小ｃｒｉｓｐｒヌクレアーゼを含むシステム、方法及び組成物 - Google Patents

遺伝子編集のため、並びにプログラム可能な遺伝子活性化及び阻害のための微小ｃｒｉｓｐｒヌクレアーゼを含むシステム、方法及び組成物 Download PDF

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JP2024522764A
JP2024522764A JP2023577655A JP2023577655A JP2024522764A JP 2024522764 A JP2024522764 A JP 2024522764A JP 2023577655 A JP2023577655 A JP 2023577655A JP 2023577655 A JP2023577655 A JP 2023577655A JP 2024522764 A JP2024522764 A JP 2024522764A
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target
acid sequence
dna
specific nuclease
composition
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JP2024522764A5 (https=
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ジアン，カイイー
ビリガー，ルーカス
アブディエ，オマー・オサマ
グーテンバーグ，ジョナサン・エス
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Massachusetts Institute of Technology
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/09—Fusion polypeptide containing a localisation/targetting motif containing a nuclear localisation signal
    • C—CHEMISTRY; METALLURGY
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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  • Wood Science & Technology (AREA)
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  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
JP2023577655A 2021-06-17 2022-06-16 遺伝子編集のため、並びにプログラム可能な遺伝子活性化及び阻害のための微小ｃｒｉｓｐｒヌクレアーゼを含むシステム、方法及び組成物 Pending JP2024522764A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163211610P 2021-06-17 2021-06-17
US63/211,610 2021-06-17
PCT/US2022/033749 WO2022266298A1 (en) 2021-06-17 2022-06-16 Systems, methods, and compositions comprising miniature crispr nucleases for gene editing and programmable gene activation and inhibition

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JP2024522764A true JP2024522764A (ja) 2024-06-21
JP2024522764A5 JP2024522764A5 (https=) 2025-06-23

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US (1) US20240309348A1 (https=)
EP (1) EP4355869A1 (https=)
JP (1) JP2024522764A (https=)
AU (1) AU2022292659A1 (https=)
CA (1) CA3223009A1 (https=)
WO (1) WO2022266298A1 (https=)

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CN116376874B (zh) * 2023-03-24 2025-07-22 尧唐(上海)生物科技有限公司 一种Cas蛋白、其基因编辑系统及应用
WO2024249770A2 (en) * 2023-06-01 2024-12-05 University Of Southern California Spatiotemporal control of genomics and epigenomics by ultrasound
AU2024357941A1 (en) * 2023-10-09 2026-04-02 Pairwise Plants Services, Inc. Circular permutants, compositions comprising the same, and methods of use thereof
WO2026019466A1 (en) * 2024-07-17 2026-01-22 Massachusetts Institute Of Technology Engineered t7 rna polymerases for improved rna production

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020123887A2 (en) * 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing

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US4554A (en) 1846-05-30 Island
US101A (en) 1836-12-06 Method of jcakibtg and furling iw sails fob ships
KR102929494B1 (ko) * 2018-10-29 2026-02-20 차이나 어그리컬처럴 유니버시티 신규 CRISPR/Cas12f 효소 및 시스템
BR112021020770A2 (pt) * 2019-04-18 2022-01-04 Pioneer Hi Bred Int Fatores de embriogênese para reprogramação celular de uma célula vegetal
CN114846146B (zh) * 2019-10-29 2024-04-12 基恩科雷有限责任公司 用于增加CRISPR/Cas12f1系统的效率的工程化引导RNA及其用途
CA3192654A1 (en) * 2020-09-01 2022-03-10 The Board Of Trustees Of The Leland Stanford Junior Unversity Synthetic miniature crispr-cas (casmini) system for eukaryotic genome engineering
EP4227411A4 (en) * 2020-10-08 2025-07-09 Genkore Inc MODIFIED GUIDE RNA TO INCREASE THE EFFICIENCY OF THE CRISPR/CAS12F1 SYSTEM, AND ITS USE
WO2022075808A1 (ko) * 2020-10-08 2022-04-14 주식회사 진코어 Crispr/cas12f1 시스템 효율화를 위한 u-rich tail을 포함하는 엔지니어링 된 가이드 rna 및 그 용도

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2020123887A2 (en) * 2018-12-14 2020-06-18 Pioneer Hi-Bred International, Inc. Novel crispr-cas systems for genome editing

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AU2022292659A1 (en) 2023-12-21
US20240309348A1 (en) 2024-09-19
WO2022266298A1 (en) 2022-12-22
EP4355869A1 (en) 2024-04-24
CA3223009A1 (en) 2022-12-22

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