CA3208416A1 - Methods of producing haemogenic endothelial cells from pluripotent stem cells - Google Patents

Methods of producing haemogenic endothelial cells from pluripotent stem cells Download PDF

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Publication number
CA3208416A1
CA3208416A1 CA3208416A CA3208416A CA3208416A1 CA 3208416 A1 CA3208416 A1 CA 3208416A1 CA 3208416 A CA3208416 A CA 3208416A CA 3208416 A CA3208416 A CA 3208416A CA 3208416 A1 CA3208416 A1 CA 3208416A1
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cells
induction medium
mesoderm
population
hecs
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CA3208416A
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English (en)
French (fr)
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Eva GARCIA-ALEGRIA
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Adaptimmune Ltd
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Adaptimmune Ltd
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0636—T lymphocytes
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/44—Vessels; Vascular smooth muscle cells; Endothelial cells; Endothelial progenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/10—Cellular immunotherapy characterised by the cell type used
    • A61K40/11—T-cells, e.g. tumour infiltrating lymphocytes [TIL] or regulatory T [Treg] cells; Lymphokine-activated killer [LAK] cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/31—Chimeric antigen receptors [CAR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/30—Cellular immunotherapy characterised by the recombinant expression of specific molecules in the cells of the immune system
    • A61K40/32—T-cell receptors [TCR]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K40/00—Cellular immunotherapy
    • A61K40/40—Cellular immunotherapy characterised by antigens that are targeted or presented by cells of the immune system
    • A61K40/41—Vertebrate antigens
    • A61K40/42—Cancer antigens
    • A61K40/428—Undefined tumor antigens, e.g. tumor lysate or antigens targeted by cells isolated from tumor
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0647—Haematopoietic stem cells; Uncommitted or multipotent progenitors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
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    • C12N2501/20—Cytokines; Chemokines
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
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  • Health & Medical Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • Wood Science & Technology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Vascular Medicine (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA3208416A 2021-02-18 2022-02-17 Methods of producing haemogenic endothelial cells from pluripotent stem cells Pending CA3208416A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GBGB2102297.5A GB202102297D0 (en) 2021-02-18 2021-02-18 Methods of Producing Haemogenic Endothelial Cells from Pluripotent Stem Cells
GB2102297.5 2021-02-18
PCT/EP2022/053985 WO2022175401A1 (en) 2021-02-18 2022-02-17 Methods of producing haemogenic endothelial cells from pluripotent stem cells

Publications (1)

Publication Number Publication Date
CA3208416A1 true CA3208416A1 (en) 2022-08-25

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ID=75339190

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3208416A Pending CA3208416A1 (en) 2021-02-18 2022-02-17 Methods of producing haemogenic endothelial cells from pluripotent stem cells

Country Status (9)

Country Link
US (1) US20240124841A1 (https=)
EP (1) EP4294911A1 (https=)
JP (1) JP2024507472A (https=)
CN (1) CN116917465A (https=)
AU (1) AU2022223203A1 (https=)
CA (1) CA3208416A1 (https=)
GB (1) GB202102297D0 (https=)
IL (1) IL305018A (https=)
WO (1) WO2022175401A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201911958D0 (en) * 2019-08-20 2019-10-02 Adaptimmune Ltd Methods of t cell production
GB202205572D0 (en) 2022-04-14 2022-06-01 Adaptimmune Ltd Engineered T cells
CN115247152B (zh) * 2022-09-21 2022-12-27 呈诺再生医学科技(北京)有限公司 制备造血干细胞或造血干祖细胞的方法及培养长期再生造血干细胞的方法
CN118360247A (zh) * 2023-01-19 2024-07-19 中国科学院动物研究所 一种iSCLP细胞及其制备方法和应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8652845B2 (en) * 2010-03-18 2014-02-18 Kyoto University Method for producing mesodermal cells by culturing under adherent conditions and without co-culture with cells from a different species in a serum-free medium
HUE043785T2 (hu) * 2013-03-13 2019-09-30 Wisconsin Alumni Res Found Módszerek és anyagok humán pluripotens õssejtek hematoendothelialis differenciálására meghatározott körülmények között
US10385313B2 (en) * 2013-10-01 2019-08-20 The Usa, As Represented By The Secretary, Department Of Health And Human Services Human IPSC-derived vascular-related and hematopoetic cells for therapies and toxicology/drug screenings
WO2015199127A1 (ja) * 2014-06-24 2015-12-30 国立大学法人京都大学 中胚葉細胞および造血細胞の製造方法
WO2016123100A1 (en) * 2015-01-26 2016-08-04 Fate Therapeutics, Inc. Methods and compositions for inducing hematopoietic cell differentiation
SG11201803145RA (en) * 2015-11-04 2018-05-30 Fate Therapeutics Inc Methods and compositions for inducing hematopoietic cell differentiation
CN112041428B (zh) * 2018-01-18 2024-05-24 新加坡科技研究局 用于在悬浮培养物中分化人多能干细胞系的方法
AU2019368313A1 (en) * 2018-10-24 2021-05-20 Hebecell Corporation Methods and systems for manufacturing hematopoietic lineage cells
GB201911957D0 (en) * 2019-08-20 2019-10-02 Adaptimmune Ltd Methods of producing haemogenic progenitor cells from pluripotent stem cells
EP4017969A1 (en) * 2019-08-20 2022-06-29 Adaptimmune Limited Culture medium for haematopoietic induction

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Publication number Publication date
US20240124841A1 (en) 2024-04-18
EP4294911A1 (en) 2023-12-27
CN116917465A (zh) 2023-10-20
WO2022175401A1 (en) 2022-08-25
JP2024507472A (ja) 2024-02-20
GB202102297D0 (en) 2021-04-07
IL305018A (en) 2023-10-01
AU2022223203A1 (en) 2023-09-07

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