CA3164017A1 - Live cell constructs for production of cultured milk product and methods using the same - Google Patents

Live cell constructs for production of cultured milk product and methods using the same Download PDF

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Publication number
CA3164017A1
CA3164017A1 CA3164017A CA3164017A CA3164017A1 CA 3164017 A1 CA3164017 A1 CA 3164017A1 CA 3164017 A CA3164017 A CA 3164017A CA 3164017 A CA3164017 A CA 3164017A CA 3164017 A1 CA3164017 A1 CA 3164017A1
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Prior art keywords
cells
mammary
live
bioreactor
scaffold
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Pending
Application number
CA3164017A
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English (en)
French (fr)
Inventor
Leila Strickland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomilq Inc
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Biomilq Inc
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=74095691&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA3164017(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Biomilq Inc filed Critical Biomilq Inc
Publication of CA3164017A1 publication Critical patent/CA3164017A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0631—Mammary cells
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C23/00—Other dairy products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; PREPARATION THEREOF
    • A23C9/00—Milk preparations; Milk powder or milk powder preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A61K35/20—Milk; Whey; Colostrum
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/02—Atmosphere, e.g. low oxygen conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/315—Prolactin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2513/00—3D culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2523/00—Culture process characterised by temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/52—Fibronectin; Laminin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins
    • C12N2533/54—Collagen; Gelatin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/72—Chitin, chitosan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/74—Alginate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/76—Agarose, agar-agar
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/78—Cellulose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/70—Polysaccharides
    • C12N2533/80—Hyaluronan
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Dermatology (AREA)
  • Sustainable Development (AREA)
  • Immunology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Veterinary Medicine (AREA)
  • Molecular Biology (AREA)
  • Clinical Laboratory Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA3164017A 2020-01-08 2020-12-18 Live cell constructs for production of cultured milk product and methods using the same Pending CA3164017A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202062958407P 2020-01-08 2020-01-08
US62/958,407 2020-01-08
US202063199164P 2020-12-10 2020-12-10
US63/199,164 2020-12-10
PCT/US2020/066209 WO2021141762A1 (en) 2020-01-08 2020-12-18 Live cell constructs for production of cultured milk product and methods using the same

Publications (1)

Publication Number Publication Date
CA3164017A1 true CA3164017A1 (en) 2021-07-15

Family

ID=74095691

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3164017A Pending CA3164017A1 (en) 2020-01-08 2020-12-18 Live cell constructs for production of cultured milk product and methods using the same

Country Status (12)

Country Link
US (3) US11111477B2 (https=)
EP (3) EP3848453A1 (https=)
JP (2) JP7759326B2 (https=)
KR (1) KR20220125295A (https=)
CN (2) CN115279892B (https=)
AU (1) AU2020420671A1 (https=)
BR (1) BR112022013569A2 (https=)
CA (1) CA3164017A1 (https=)
ES (1) ES2909832T3 (https=)
IL (1) IL294496A (https=)
MX (1) MX2022008483A (https=)
WO (1) WO2021141762A1 (https=)

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IL294496A (en) 2020-01-08 2022-09-01 Biomilq Inc Structures suitable for living for the production of a cultured milk product and methods for use
AU2021205324A1 (en) * 2020-01-08 2022-08-18 Shayne Guiliano Live cell constructs for biosynthetic milk production and related products and methods
US11206843B1 (en) 2020-05-26 2021-12-28 BIOMILQ, Inc. Milk product compositions
US11236299B1 (en) * 2020-09-08 2022-02-01 Biomilk Ltd. Methods and systems for in-vitro milk production
US20250223550A1 (en) * 2021-10-27 2025-07-10 Societe Des Produits Nestle S.A. Method for producing milk like products
WO2023097012A1 (en) * 2021-11-23 2023-06-01 BIOMILQ, Inc. Milk product compositions
KR20240114773A (ko) 2021-12-14 2024-07-24 인바이오스 엔.브이. 알파-1,3-푸코실화된 화합물의 생산
US20240417768A1 (en) 2021-12-14 2024-12-19 Inbiose N.V. Production of alpha-1,4-fucosylated compounds
AU2022416822A1 (en) 2021-12-15 2024-07-11 Inbiose N.V. Sialyltransferases for the production of sialylated oligosaccharides
WO2023137465A1 (en) * 2022-01-13 2023-07-20 BIOMILQ, Inc. Optimization of live cell constructs for production of cultured milk product and methods of using the same
WO2023175079A1 (en) 2022-03-16 2023-09-21 Inbiose N.V. Sialyltransferases for the production of sialylated oligosaccharides
AU2023246643A1 (en) 2022-04-01 2024-10-10 Inbiose N.V. Sialyltransferases for the production of sialylated oligosaccharides
IL318164A (en) * 2022-07-05 2025-03-01 Senara Gmbh Pulsed flow culture of milk-producing cell types for milk secretion
WO2024047096A1 (en) 2022-08-30 2024-03-07 Inbiose N.V. Process for purification of an oligosaccharide
WO2024165525A1 (en) 2023-02-06 2024-08-15 Inbiose N.V. Production of a saccharide, lactobionic acid and/or glycosylated forms of lactobionic acid
CN120641559A (zh) 2023-02-07 2025-09-12 因比奥斯公司 由具有降低的UDP-GlcNAc合成的细胞来产生二糖和/或乳寡糖
WO2024223815A1 (en) 2023-04-26 2024-10-31 Inbiose N.V. Production of a negatively charged oligosaccharide by a cell
EP4720280A2 (en) * 2023-06-02 2026-04-08 Biomilq, Inc. Exosome compositions and methods of use
WO2024261312A2 (en) 2023-06-21 2024-12-26 Inbiose N.V. Sialyltransferases for the production of sialylated oligosaccharides
WO2025088103A1 (en) 2023-10-26 2025-05-01 Inbiose N.V. Production of a sialylated compound
WO2025088102A1 (en) 2023-10-26 2025-05-01 Inbiose N.V. PRODUCTION OF A SIALYLATED OLIGOSACCHARIDE COMPRISING GAL-β1,3-[NEU5AC-A2,6]-HEXNAC-R
WO2025120607A1 (en) * 2023-12-08 2025-06-12 Senara Gmbh Cell-cultivated milk for confectionery and dairy product uses
EP4570821A1 (en) * 2023-12-11 2025-06-18 Senara GmbH Milk producing cells
WO2025172603A1 (en) 2024-02-16 2025-08-21 Inbiose N.V. Method for importing a disaccharide inside a cell
WO2025224348A1 (en) 2024-04-26 2025-10-30 Inbiose N.V. Production of a milk oligosaccharide mixture
WO2025262230A1 (en) 2024-06-19 2025-12-26 Mumilk In vitro method of producing milk
WO2026052789A1 (en) 2024-09-05 2026-03-12 Inbiose N.V. Fucosyltransferase mutants
CN120591197A (zh) * 2025-05-30 2025-09-05 中国农业大学 一种奶牛乳腺上皮细胞3d球体模型的建立方法及应用

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IL294496A (en) 2020-01-08 2022-09-01 Biomilq Inc Structures suitable for living for the production of a cultured milk product and methods for use
AU2021205324A1 (en) 2020-01-08 2022-08-18 Shayne Guiliano Live cell constructs for biosynthetic milk production and related products and methods
WO2022115033A1 (en) 2020-11-24 2022-06-02 Turtletree Labs Pte. Ltd. Bioreactor systems and methods for culturing cells

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Publication number Publication date
CN115279892B (zh) 2025-09-23
IL294496A (en) 2022-09-01
CN121227621A (zh) 2025-12-30
US20250179431A1 (en) 2025-06-05
WO2021141762A1 (en) 2021-07-15
ES2909832T3 (es) 2022-05-10
JP2026021344A (ja) 2026-02-10
US20210207090A1 (en) 2021-07-08
EP3848454B1 (en) 2022-02-09
US20210403865A1 (en) 2021-12-30
KR20220125295A (ko) 2022-09-14
BR112022013569A2 (pt) 2022-09-13
JP7759326B2 (ja) 2025-10-23
US11111477B2 (en) 2021-09-07
EP4052583A1 (en) 2022-09-07
JP2023515749A (ja) 2023-04-14
EP3848453A1 (en) 2021-07-14
MX2022008483A (es) 2022-09-07
AU2020420671A1 (en) 2022-07-28
CN115279892A (zh) 2022-11-01
EP3848454A1 (en) 2021-07-14

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