CA3146551A1 - Process for isolating a high purity protein preparation from plant material and products thereof - Google Patents

Process for isolating a high purity protein preparation from plant material and products thereof Download PDF

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Publication number
CA3146551A1
CA3146551A1 CA3146551A CA3146551A CA3146551A1 CA 3146551 A1 CA3146551 A1 CA 3146551A1 CA 3146551 A CA3146551 A CA 3146551A CA 3146551 A CA3146551 A CA 3146551A CA 3146551 A1 CA3146551 A1 CA 3146551A1
Authority
CA
Canada
Prior art keywords
plant material
liquid phase
purified protein
lysed
chlorophyll
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3146551A
Other languages
English (en)
French (fr)
Inventor
Tony MARTENS
Cesar Flores
Eduardo REYNOSO MORENO
Roland VAN DER MOLEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Plantible Foods Inc
Original Assignee
Plantible Foods Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Plantible Foods Inc filed Critical Plantible Foods Inc
Publication of CA3146551A1 publication Critical patent/CA3146551A1/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/185—Vegetable proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/145—Extraction; Separation; Purification by extraction or solubilisation
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J1/00—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites
    • A23J1/006—Obtaining protein compositions for foodstuffs; Bulk opening of eggs and separation of yolks from whites from vegetable materials
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23J—PROTEIN COMPOSITIONS FOR FOODSTUFFS; WORKING-UP PROTEINS FOR FOODSTUFFS; PHOSPHATIDE COMPOSITIONS FOR FOODSTUFFS
    • A23J3/00—Working-up of proteins for foodstuffs
    • A23J3/30—Working-up of proteins for foodstuffs by hydrolysis
    • A23J3/32—Working-up of proteins for foodstuffs by hydrolysis using chemical agents
    • A23J3/34—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes
    • A23J3/346—Working-up of proteins for foodstuffs by hydrolysis using chemical agents using enzymes of vegetable proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/32—Extraction; Separation; Purification by precipitation as complexes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01039—Ribulose-bisphosphate carboxylase (4.1.1.39)
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Nutrition Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Water Supply & Treatment (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Botany (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
CA3146551A 2019-07-11 2020-07-10 Process for isolating a high purity protein preparation from plant material and products thereof Pending CA3146551A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962872917P 2019-07-11 2019-07-11
US62/872,917 2019-07-11
PCT/US2020/041525 WO2021007484A1 (en) 2019-07-11 2020-07-10 Process for isolating a high purity protein preparation from plant material and products thereof

Publications (1)

Publication Number Publication Date
CA3146551A1 true CA3146551A1 (en) 2021-01-14

Family

ID=74114787

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3146551A Pending CA3146551A1 (en) 2019-07-11 2020-07-10 Process for isolating a high purity protein preparation from plant material and products thereof

Country Status (9)

Country Link
US (3) US20220259259A1 (enExample)
EP (1) EP3997102A4 (enExample)
JP (2) JP7646624B2 (enExample)
CN (2) CN114364688A (enExample)
AU (1) AU2020310192A1 (enExample)
CA (1) CA3146551A1 (enExample)
MX (1) MX2022000315A (enExample)
MY (1) MY208163A (enExample)
WO (1) WO2021007484A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111995656B (zh) * 2020-09-11 2022-09-06 宁波工程学院 一种植物蛋白的提取方法
PT117122A (pt) * 2021-03-17 2022-11-30 Inst Superior Agronomia Processo de obtenção de rubisco pura a partir de um material fotossintético
WO2023039419A2 (en) 2021-09-08 2023-03-16 Plantible Foods Inc. Systems and methods for measuring mat density of aquatic biomass
EP4415565A4 (en) * 2021-10-13 2025-08-13 Plantible Foods Inc USE OF RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE/OXYGENASE ISOLATE (RUBISCO) AS A FAT-BINDING AGENT
EP4205552A1 (en) 2021-12-30 2023-07-05 BK Giulini GmbH Meat and seafood analogue products
CA3242345A1 (en) 2021-12-30 2023-07-06 Bk Giulini Gmbh Meat and seafood analogue products
WO2023137391A2 (en) * 2022-01-14 2023-07-20 Plantible Foods Inc. Directional freezing of ribulose-1,5-bisphosphate carboxylate-oxygenase (rubisco)
EP4476334A1 (en) * 2022-02-10 2024-12-18 Instituto Superior de Agronomia - Universidade de Lisboa Process of obtaining a protein preparation
EP4353087A1 (en) 2022-10-11 2024-04-17 BK Giulini GmbH Meat analogue products
US20260137100A1 (en) 2022-10-11 2026-05-21 Bk Giulini Gmbh Meat analogue products
WO2024110444A1 (en) 2022-11-21 2024-05-30 Chreto Aps Method for purification of a target product using affinity purification technology
WO2025048704A1 (en) 2023-08-31 2025-03-06 Chalmers Ventures Ab A process for extracting proteins from photosynthetic biomass
WO2026125661A1 (en) 2024-12-11 2026-06-18 Bk Giulini Gmbh Compositions for acidified dairy analogue products
EP4767827A1 (en) 2024-12-24 2026-07-01 BK Giulini GmbH COMPOSITIONS FOR ACIDIFIED DAIRY PRODUCT ANALOGUES
CN119371569B (zh) * 2024-12-31 2025-03-11 山东盈莱旺农业科技有限公司 一种百合多糖的提取方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1430566A (en) * 1974-11-15 1976-03-31 Nestel Sa Isolation of proteins
WO2008143914A1 (en) * 2007-05-16 2008-11-27 The University Of Maryland, College Park Novel methods for recovery of leaf proteins
WO2011078671A1 (en) * 2009-12-22 2011-06-30 Nizo Food Research B.V. Process for isolating a dechlorophylllized rubisco preparation from a plant material
BR112012023444A2 (pt) * 2010-03-17 2017-10-03 Pa Llc Método e sistema para processamento de espécie aquática
CA2858353A1 (en) * 2011-12-22 2013-06-27 Xyleco, Inc. Biomass processing
EP2682168A1 (en) * 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
US11284632B2 (en) * 2012-12-24 2022-03-29 Stichting Wageningen Research Economical process for the isolation of functional protein from plants
CA2911195A1 (en) * 2013-04-05 2014-10-09 Mo Bio Laboratories, Inc. Kits and methods for isolating protein from biological and environmental samples
US20190144497A1 (en) * 2016-07-07 2019-05-16 Hinoman Ltd. Concentrated protein materials from de-chlorophyllized aquatic plant biomass
US10757964B2 (en) * 2017-07-20 2020-09-01 R.J. Reynolds Tobacco Company Purification of tobacco-derived protein compositions

Also Published As

Publication number Publication date
JP2025084972A (ja) 2025-06-03
EP3997102A1 (en) 2022-05-18
US20230217981A1 (en) 2023-07-13
EP3997102A4 (en) 2023-08-16
US20220259259A1 (en) 2022-08-18
JP7646624B2 (ja) 2025-03-17
CN120081893A (zh) 2025-06-03
MY208163A (en) 2025-04-21
MX2022000315A (es) 2022-04-20
WO2021007484A1 (en) 2021-01-14
CN114364688A (zh) 2022-04-15
JP2022541399A (ja) 2022-09-26
AU2020310192A1 (en) 2022-02-03
US20220232877A1 (en) 2022-07-28

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