CA3088891C - Novel primers and uses thereof - Google Patents
Novel primers and uses thereofInfo
- Publication number
- CA3088891C CA3088891C CA3088891A CA3088891A CA3088891C CA 3088891 C CA3088891 C CA 3088891C CA 3088891 A CA3088891 A CA 3088891A CA 3088891 A CA3088891 A CA 3088891A CA 3088891 C CA3088891 C CA 3088891C
- Authority
- CA
- Canada
- Prior art keywords
- section
- target
- adaptor
- specific
- stem
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/30—Oligonucleotides characterised by their secondary structure
- C12Q2525/301—Hairpin oligonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/179—Nucleic acid detection characterized by the use of physical, structural and functional properties the label being a nucleic acid
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA3283087A CA3283087A1 (en) | 2018-01-12 | 2019-01-11 | Novel primers and uses thereof |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862617066P | 2018-01-12 | 2018-01-12 | |
| US62/617,066 | 2018-01-12 | ||
| PCT/US2019/013346 WO2019140298A1 (en) | 2018-01-12 | 2019-01-11 | Novel primers and uses thereof |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3283087A Division CA3283087A1 (en) | 2018-01-12 | 2019-01-11 | Novel primers and uses thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA3088891A1 CA3088891A1 (en) | 2019-07-18 |
| CA3088891C true CA3088891C (en) | 2026-03-10 |
Family
ID=65269117
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3088891A Active CA3088891C (en) | 2018-01-12 | 2019-01-11 | Novel primers and uses thereof |
| CA3283087A Pending CA3283087A1 (en) | 2018-01-12 | 2019-01-11 | Novel primers and uses thereof |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3283087A Pending CA3283087A1 (en) | 2018-01-12 | 2019-01-11 | Novel primers and uses thereof |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20210071246A1 (https=) |
| EP (3) | EP4671380A3 (https=) |
| JP (3) | JP7322063B2 (https=) |
| CN (2) | CN121653234A (https=) |
| CA (2) | CA3088891C (https=) |
| WO (1) | WO2019140298A1 (https=) |
Families Citing this family (41)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9424392B2 (en) | 2005-11-26 | 2016-08-23 | Natera, Inc. | System and method for cleaning noisy genetic data from target individuals using genetic data from genetically related individuals |
| US11111543B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| US11111544B2 (en) | 2005-07-29 | 2021-09-07 | Natera, Inc. | System and method for cleaning noisy genetic data and determining chromosome copy number |
| WO2011041485A1 (en) | 2009-09-30 | 2011-04-07 | Gene Security Network, Inc. | Methods for non-invasive prenatal ploidy calling |
| US12221653B2 (en) | 2010-05-18 | 2025-02-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US9677118B2 (en) | 2014-04-21 | 2017-06-13 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11939634B2 (en) | 2010-05-18 | 2024-03-26 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US12152275B2 (en) | 2010-05-18 | 2024-11-26 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US11332793B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11322224B2 (en) | 2010-05-18 | 2022-05-03 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| WO2011146632A1 (en) | 2010-05-18 | 2011-11-24 | Gene Security Network Inc. | Methods for non-invasive prenatal ploidy calling |
| US11332785B2 (en) | 2010-05-18 | 2022-05-17 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US20190010543A1 (en) | 2010-05-18 | 2019-01-10 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11326208B2 (en) | 2010-05-18 | 2022-05-10 | Natera, Inc. | Methods for nested PCR amplification of cell-free DNA |
| US11339429B2 (en) | 2010-05-18 | 2022-05-24 | Natera, Inc. | Methods for non-invasive prenatal ploidy calling |
| US10316362B2 (en) | 2010-05-18 | 2019-06-11 | Natera, Inc. | Methods for simultaneous amplification of target loci |
| US11408031B2 (en) | 2010-05-18 | 2022-08-09 | Natera, Inc. | Methods for non-invasive prenatal paternity testing |
| CN103608466B (zh) | 2010-12-22 | 2020-09-18 | 纳特拉公司 | 非侵入性产前亲子鉴定方法 |
| RU2671980C2 (ru) | 2011-02-09 | 2018-11-08 | Натера, Инк. | Способы неинвазивного пренатального установления плоидности |
| US20140100126A1 (en) | 2012-08-17 | 2014-04-10 | Natera, Inc. | Method for Non-Invasive Prenatal Testing Using Parental Mosaicism Data |
| US10577655B2 (en) | 2013-09-27 | 2020-03-03 | Natera, Inc. | Cell free DNA diagnostic testing standards |
| CA2945962C (en) | 2014-04-21 | 2023-08-29 | Natera, Inc. | Detecting mutations and ploidy in chromosomal segments |
| US20180173846A1 (en) | 2014-06-05 | 2018-06-21 | Natera, Inc. | Systems and Methods for Detection of Aneuploidy |
| US11479812B2 (en) | 2015-05-11 | 2022-10-25 | Natera, Inc. | Methods and compositions for determining ploidy |
| RU2760913C2 (ru) | 2016-04-15 | 2021-12-01 | Натера, Инк. | Способы выявления рака легкого |
| WO2018067517A1 (en) | 2016-10-04 | 2018-04-12 | Natera, Inc. | Methods for characterizing copy number variation using proximity-litigation sequencing |
| GB201618485D0 (en) | 2016-11-02 | 2016-12-14 | Ucl Business Plc | Method of detecting tumour recurrence |
| US10011870B2 (en) | 2016-12-07 | 2018-07-03 | Natera, Inc. | Compositions and methods for identifying nucleic acid molecules |
| US12084720B2 (en) | 2017-12-14 | 2024-09-10 | Natera, Inc. | Assessing graft suitability for transplantation |
| US12024738B2 (en) | 2018-04-14 | 2024-07-02 | Natera, Inc. | Methods for cancer detection and monitoring |
| US12234509B2 (en) | 2018-07-03 | 2025-02-25 | Natera, Inc. | Methods for detection of donor-derived cell-free DNA |
| US12305235B2 (en) | 2019-06-06 | 2025-05-20 | Natera, Inc. | Methods for detecting immune cell DNA and monitoring immune system |
| EP4017989B1 (en) * | 2019-08-20 | 2024-12-18 | Illumina, Inc. | Hairpin primer design for sequential pcr production of targeted sequencing libraries |
| MX2023001400A (es) * | 2020-09-11 | 2023-04-25 | Illumina Cambridge Ltd | Metodos para enriquecer una secuencia diana a partir de una genoteca de secuenciación usando adaptadores de horquilla. |
| WO2022223561A1 (en) * | 2021-04-20 | 2022-10-27 | Simsen Diagnostics Ab | Compositions and methods for cell-free nucleic acid isolation |
| WO2023067110A1 (en) * | 2021-10-20 | 2023-04-27 | Tataa Biocenter Ab | Methods and compositions for detection of mutant nucleic acid sequences |
| IL314003A (en) * | 2021-12-30 | 2024-11-01 | Cue Health Inc | Compounds and methods for isothermal amplification of nucleic acids |
| US20250361550A1 (en) * | 2022-08-15 | 2025-11-27 | Simsen Diagnostics Ab | Nucleic acid amplification |
| WO2024170770A1 (en) | 2023-02-16 | 2024-08-22 | Asecud Sa | High nucleic acid sequence discrimination using a multifaceted nucleic acid molecule |
| EP4488384A1 (en) * | 2023-07-05 | 2025-01-08 | Infiniplex Ltd. | Primers for selective amplification of rare target sequences |
| EP4520840A1 (en) * | 2023-09-05 | 2025-03-12 | Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. | Suppression pcr-based selective enrichment sequencing |
Family Cites Families (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7108976B2 (en) * | 2002-06-17 | 2006-09-19 | Affymetrix, Inc. | Complexity management of genomic DNA by locus specific amplification |
| JP4879975B2 (ja) * | 2005-05-31 | 2012-02-22 | アプライド バイオシステムズ リミテッド ライアビリティー カンパニー | 短い核酸のマルチプレックス増幅 |
| EP2308990B1 (en) | 2005-07-15 | 2012-09-26 | Life Technologies Corporation | Analyzing messenger RNA and micro RNA in the same reaction mixture |
| US8415099B2 (en) * | 2007-11-05 | 2013-04-09 | Complete Genomics, Inc. | Efficient base determination in sequencing reactions |
| EP2633071B1 (en) | 2010-10-27 | 2016-10-12 | President and Fellows of Harvard College | Compositions of toehold primer duplexes and methods of use |
| US9163329B2 (en) * | 2010-11-12 | 2015-10-20 | Agilent Technologies, Inc. | RNA labeling method |
| CA2877493C (en) * | 2012-07-24 | 2020-08-25 | Natera, Inc. | Highly multiplex pcr methods and compositions |
| KR102351434B1 (ko) * | 2013-02-07 | 2022-01-17 | 러트거즈,더스테이트유니버시티오브뉴저지 | 고도로 선택적인 핵산 증폭 프라이머 |
| US11473140B2 (en) * | 2013-11-26 | 2022-10-18 | Lc Sciences Lc | Highly selective omega primer amplification of nucleic acid sequences |
| US20170349926A1 (en) * | 2014-12-22 | 2017-12-07 | DNAe Group Holdings LTD. | Bubble primers |
| US10557134B2 (en) * | 2015-02-24 | 2020-02-11 | Trustees Of Boston University | Protection of barcodes during DNA amplification using molecular hairpins |
| EP3302089B1 (en) * | 2015-06-01 | 2023-08-16 | GEA Food Solutions Weert B.V. | Method for coating a lollipop |
| EP3440226B1 (en) * | 2016-04-07 | 2021-02-17 | Rutgers, The State University of New Jersey | Multiplex nucleic acid assay methods capable of detecting closely related alleles, and reagents therefor |
| CN107058310A (zh) * | 2016-08-12 | 2017-08-18 | 艾吉泰康生物科技(北京)有限公司 | 一种提高基因低频突变检测灵敏度的扩增子文库构建方法 |
| CN106282353B (zh) * | 2016-08-26 | 2019-12-10 | 上海翼和应用生物技术有限公司 | 一种利用发夹引物进行多重pcr的方法 |
| CN107365769B (zh) * | 2017-07-25 | 2021-05-04 | 深圳华大智造科技股份有限公司 | 一种鼓泡状引物及其组成的试剂盒和应用 |
-
2019
- 2019-01-11 WO PCT/US2019/013346 patent/WO2019140298A1/en not_active Ceased
- 2019-01-11 CN CN202511678455.3A patent/CN121653234A/zh active Pending
- 2019-01-11 JP JP2020558864A patent/JP7322063B2/ja active Active
- 2019-01-11 CA CA3088891A patent/CA3088891C/en active Active
- 2019-01-11 EP EP25214405.0A patent/EP4671380A3/en active Pending
- 2019-01-11 CA CA3283087A patent/CA3283087A1/en active Pending
- 2019-01-11 EP EP19702785.7A patent/EP3737773B1/en active Active
- 2019-01-11 CN CN201980008237.5A patent/CN111699269B/zh active Active
- 2019-01-11 EP EP23167431.8A patent/EP4245863B1/en active Active
- 2019-01-11 US US16/959,949 patent/US20210071246A1/en active Pending
-
2023
- 2023-07-26 JP JP2023121655A patent/JP7773511B2/ja active Active
-
2025
- 2025-11-07 JP JP2025188258A patent/JP2026016789A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4245863A2 (en) | 2023-09-20 |
| CN111699269A (zh) | 2020-09-22 |
| CN111699269B (zh) | 2025-12-05 |
| JP2021510541A (ja) | 2021-04-30 |
| CN121653234A (zh) | 2026-03-13 |
| EP3737773B1 (en) | 2023-04-12 |
| EP4671380A3 (en) | 2026-02-25 |
| JP7773511B2 (ja) | 2025-11-19 |
| EP4671380A2 (en) | 2025-12-31 |
| US20210071246A1 (en) | 2021-03-11 |
| EP4245863B1 (en) | 2026-03-18 |
| EP4245863A3 (en) | 2024-01-24 |
| JP2026016789A (ja) | 2026-02-03 |
| WO2019140298A1 (en) | 2019-07-18 |
| JP7322063B2 (ja) | 2023-08-07 |
| JP2023139220A (ja) | 2023-10-03 |
| CA3283087A1 (en) | 2026-03-02 |
| EP3737773A1 (en) | 2020-11-18 |
| CA3088891A1 (en) | 2019-07-18 |
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