CA3083571A1 - Selecting for developability of polypeptide drugs in eukaryotic cell display systems - Google Patents

Selecting for developability of polypeptide drugs in eukaryotic cell display systems Download PDF

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Publication number
CA3083571A1
CA3083571A1 CA3083571A CA3083571A CA3083571A1 CA 3083571 A1 CA3083571 A1 CA 3083571A1 CA 3083571 A CA3083571 A CA 3083571A CA 3083571 A CA3083571 A CA 3083571A CA 3083571 A1 CA3083571 A1 CA 3083571A1
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CA
Canada
Prior art keywords
binders
cells
clones
binder
library
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3083571A
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English (en)
French (fr)
Inventor
John Mccafferty
Rajika PERERA
Michael Richard Dyson
Kothai PARTHIBAN
Johanna Liinamaria SYRJANEN
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Iontas Ltd
Original Assignee
Iontas Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Iontas Ltd filed Critical Iontas Ltd
Publication of CA3083571A1 publication Critical patent/CA3083571A1/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034Isolating an individual clone by screening libraries
    • C12N15/1037Screening libraries presented on the surface of microorganisms, e.g. phage display, E. coli display
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102Mutagenizing nucleic acids

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3083571A 2017-12-06 2018-12-05 Selecting for developability of polypeptide drugs in eukaryotic cell display systems Pending CA3083571A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB1720351.4 2017-12-06
GBGB1720351.4A GB201720351D0 (en) 2017-12-06 2017-12-06 Selecting for developability in drug discovery
PCT/EP2018/083698 WO2019110691A1 (en) 2017-12-06 2018-12-05 Selecting for developability of polypeptide drugs in eukaryotic cell display systems

Publications (1)

Publication Number Publication Date
CA3083571A1 true CA3083571A1 (en) 2019-06-13

Family

ID=60950392

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3083571A Pending CA3083571A1 (en) 2017-12-06 2018-12-05 Selecting for developability of polypeptide drugs in eukaryotic cell display systems

Country Status (8)

Country Link
US (1) US11499150B2 (enExample)
EP (1) EP3720959A1 (enExample)
JP (2) JP7744746B2 (enExample)
CN (1) CN111448314B (enExample)
AU (1) AU2018379426B2 (enExample)
CA (1) CA3083571A1 (enExample)
GB (1) GB201720351D0 (enExample)
WO (1) WO2019110691A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3786292A1 (en) * 2016-01-08 2021-03-03 Maxion Therapeutics Limited Binding members with altered diversity scaffold domains
GB201720162D0 (en) * 2017-12-04 2018-01-17 Univ Oxford Innovation Ltd Method
WO2021018114A1 (zh) * 2019-07-30 2021-02-04 中山康方生物医药有限公司 抗人p40蛋白域抗体及其用途
GB202014851D0 (en) 2020-09-21 2020-11-04 Iontas Ltd SARS-COV-2 antibodies
CN118525099A (zh) 2021-08-25 2024-08-20 艾恩塔斯有限公司 真核细胞中表达的蛋白质变体文库的制备
WO2023039528A1 (en) * 2021-09-10 2023-03-16 The Board Of Trustees Of The Leland Stanford Junior University Nanobody-mediated control of gene expression and epigenetic memory
US12091694B2 (en) 2022-11-18 2024-09-17 Seismic Therapeutic, Inc. Fc fusion molecules and uses thereof
EP4646476A1 (en) 2023-01-06 2025-11-12 Seismic Therapeutic, Inc. Protease variants and uses thereof

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6485943B2 (en) * 1995-01-17 2002-11-26 The University Of Chicago Method for altering antibody light chain interactions
US6300065B1 (en) * 1996-05-31 2001-10-09 Board Of Trustees Of The University Of Illinois Yeast cell surface display of proteins and uses thereof
JP2010213725A (ja) * 1998-01-20 2010-09-30 Univ Illinois タンパク質の酵母細胞表面ディスプレイおよびその使用
US20050048578A1 (en) * 2003-06-26 2005-03-03 Epitomics, Inc. Methods of screening for monoclonal antibodies with desirable activity
PT2702160T (pt) 2011-04-27 2020-07-30 Amyris Inc Métodos para modificação genómica
EP2794662A1 (en) 2011-12-22 2014-10-29 F.Hoffmann-La Roche Ag Full length antibody display system for eukaryotic cells and its use
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
GB201407852D0 (en) 2014-05-02 2014-06-18 Iontas Ltd Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules

Also Published As

Publication number Publication date
US20210163923A1 (en) 2021-06-03
AU2018379426A1 (en) 2020-06-18
US11499150B2 (en) 2022-11-15
GB201720351D0 (en) 2018-01-17
CN111448314A (zh) 2020-07-24
EP3720959A1 (en) 2020-10-14
JP7744746B2 (ja) 2025-09-26
WO2019110691A1 (en) 2019-06-13
JP2021509010A (ja) 2021-03-18
JP2024009895A (ja) 2024-01-23
CN111448314B (zh) 2024-10-18
AU2018379426B2 (en) 2025-05-22

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Effective date: 20231128