CA3081636C - Growth promoter and application thereof for prevention and treatment of clubroot on cruciferous crops - Google Patents
Growth promoter and application thereof for prevention and treatment of clubroot on cruciferous crops Download PDFInfo
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- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
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- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/10—Aromatic or araliphatic carboxylic acids, or thio analogues thereof; Derivatives thereof
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
- A01N43/36—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings
- A01N43/38—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom five-membered rings condensed with carbocyclic rings
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/14—Boron; Compounds thereof
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- A—HUMAN NECESSITIES
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- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
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Abstract
The present invention discloses a growth promoter and application thereof for prevention and treatment of club root disease of cruciferous crops. The growth promoter comprises the following components in parts by weight: 0.3-0.5 part of indolebutyric acid, 0.3-0.5 part of naphthylacetic acid, 15-20 parts of humic acid, 10-20 parts of boron and 30-50 parts of plant nutrient. The growth promoter for restoring a root absorption function provided by the present invention adopts appropriate components and proportions, the components cooperate with one another, and a simple foliar spraying method is adopted. On the one hand, it can rebuild the water and nutrient absorption function of the root system of the club root diseased plants, restore the normal growth of the diseased plants which are dying, make the survival rate of the diseased plants reach more than 90%, and increase the yield by more than 60%. On the other hand, the promoter is convenient and quick to use, overcomes the technical bottleneck that the club root diseased plants are difficult to survive, can effectively decrease labor cost by more than 80%, can simultaneously decrease the potential risks of food safety and environmental pollution caused by the use of chemical pesticides, and has remarkable social, economic and ecological benefits.
Description
GROWTH PROMOTER AND APPLICATION THEREOF FOR PREVENTION
AND TREATMENT OF CLUBROOT ON CRUCIFEROUS CROPS
Field of the Invention The present invention relates to a crop growth regulator, in particular to a growth promoter for restoring a root absorption function and application thereof for prevention and treatment of club root disease of cruciferous crops.
Background of the Invention The club root disease, commonly known as plant cancer, is a special parasitic soil-borne disease caused by infection of plasmodiophora brassicae woron, which mainly infects cruciferous plants such as oilseed rape, wild cabbage, cabbage, cauliflower, kohlrabi, leaf mustard, radish and turnip. This disease mainly damages root tissues of plants, and causes proliferation of parenchyma cells of roots to form tumors, which makes it difficult for plants to absorb nutrients, leading to wilting, slow growth and even death of plants; and when conditions are suitable, it can lead to the decline of yield and quality of cruciferous crops such as oilseed rape, or even total crop failure. According to incomplete statistics, the disease causes more than 10%
economic losses to cruciferous crops in the world, and more than 70% losses to some crops. In China, the club root disease has covered the main planting areas of oilseed rape and cruciferous vegetables. According to incomplete statistics, at present, the spread area of club root disease of oilseed rape in China is at least 600000 hectares, and the yield of oilseed rape is reduced by more than 1500kg per hectare, threatening the development of oilseed rape and cruciferous vegetable industry in China.
The club root disease is a worldwide soil-borne disease, and pathogens thereof exist in soil in the form of doirnant spores. They can adapt to extremely bad environment and survive for up to 15 years in the absence of susceptible host plants, which makes it very difficult to prevent and treat the disease. Fields with club root disease pathogens are generally not suitable for planting cruciferous plants. In addition, traditional agricultural operations, such as early sowing of oilseed rape and long-term planting of Date recue/Date received 2023-04-05 cruciferous crops in the same field, would aggravate the club root disease in the diseased field. At present, due to the lack of effective broad-spectrum club root disease resistant varieties, only chemical bactericides can be used for prevention and treatment in production, such that the amount of used chemicals is large, the costs of chemicals and labor are high, furthermore, there is no prevention and treatment effect under the case that the treatment with chemicals is performed after symptoms appear, and even worse, potential risks of environmental pollution and human health are caused by a large amount of chemicals for soil treatment. Therefore, the prominent problem that it is difficult to prevent and treat the club root disease has not only seriously hit the enthusiasm of farmers to plant cruciferous crops, but also posed a great threat to the sustainable development of oilseed rape and cruciferous vegetable industry. It is urgent to explore new strategies for prevention and treatment of club root disease.
In the previous work, the inventor of the present application has created a seed coating agent which can be used to prevent and treat the club root disease.
The prevention rate for the club root disease at the seedling stage of oilseed rape can reach more than 80%, the usage amount of pesticides is significantly reduced, the environmental affinity of the chemical prevention and treatment technology is improved, and the technical barrier that it is difficult to prevent the club root disease is overcome. However, since the seed coating agent is limited by the usage amount and efficacy duration, it is difficult to effectively prevent and control the club root disease in the later growth stage of oilseed rape, and to cure the diseased plants. If it is capable to provide a novel plant growth promoter, which is free of chemical pesticide, is environment-friendly, can rebuild or restore the nolinal nutrient and water absorption function of the root system of the club root diseased plants, restore the normal growth of the diseased plants which are dying and guarantee the yield of oilseed rape and cruciferous crops, is stable in the treatment effect on the club root disease, does not easily cause environmental pollution, and has a field use effect that is not affected by geological conditions and soil environment, not only can the normal
AND TREATMENT OF CLUBROOT ON CRUCIFEROUS CROPS
Field of the Invention The present invention relates to a crop growth regulator, in particular to a growth promoter for restoring a root absorption function and application thereof for prevention and treatment of club root disease of cruciferous crops.
Background of the Invention The club root disease, commonly known as plant cancer, is a special parasitic soil-borne disease caused by infection of plasmodiophora brassicae woron, which mainly infects cruciferous plants such as oilseed rape, wild cabbage, cabbage, cauliflower, kohlrabi, leaf mustard, radish and turnip. This disease mainly damages root tissues of plants, and causes proliferation of parenchyma cells of roots to form tumors, which makes it difficult for plants to absorb nutrients, leading to wilting, slow growth and even death of plants; and when conditions are suitable, it can lead to the decline of yield and quality of cruciferous crops such as oilseed rape, or even total crop failure. According to incomplete statistics, the disease causes more than 10%
economic losses to cruciferous crops in the world, and more than 70% losses to some crops. In China, the club root disease has covered the main planting areas of oilseed rape and cruciferous vegetables. According to incomplete statistics, at present, the spread area of club root disease of oilseed rape in China is at least 600000 hectares, and the yield of oilseed rape is reduced by more than 1500kg per hectare, threatening the development of oilseed rape and cruciferous vegetable industry in China.
The club root disease is a worldwide soil-borne disease, and pathogens thereof exist in soil in the form of doirnant spores. They can adapt to extremely bad environment and survive for up to 15 years in the absence of susceptible host plants, which makes it very difficult to prevent and treat the disease. Fields with club root disease pathogens are generally not suitable for planting cruciferous plants. In addition, traditional agricultural operations, such as early sowing of oilseed rape and long-term planting of Date recue/Date received 2023-04-05 cruciferous crops in the same field, would aggravate the club root disease in the diseased field. At present, due to the lack of effective broad-spectrum club root disease resistant varieties, only chemical bactericides can be used for prevention and treatment in production, such that the amount of used chemicals is large, the costs of chemicals and labor are high, furthermore, there is no prevention and treatment effect under the case that the treatment with chemicals is performed after symptoms appear, and even worse, potential risks of environmental pollution and human health are caused by a large amount of chemicals for soil treatment. Therefore, the prominent problem that it is difficult to prevent and treat the club root disease has not only seriously hit the enthusiasm of farmers to plant cruciferous crops, but also posed a great threat to the sustainable development of oilseed rape and cruciferous vegetable industry. It is urgent to explore new strategies for prevention and treatment of club root disease.
In the previous work, the inventor of the present application has created a seed coating agent which can be used to prevent and treat the club root disease.
The prevention rate for the club root disease at the seedling stage of oilseed rape can reach more than 80%, the usage amount of pesticides is significantly reduced, the environmental affinity of the chemical prevention and treatment technology is improved, and the technical barrier that it is difficult to prevent the club root disease is overcome. However, since the seed coating agent is limited by the usage amount and efficacy duration, it is difficult to effectively prevent and control the club root disease in the later growth stage of oilseed rape, and to cure the diseased plants. If it is capable to provide a novel plant growth promoter, which is free of chemical pesticide, is environment-friendly, can rebuild or restore the nolinal nutrient and water absorption function of the root system of the club root diseased plants, restore the normal growth of the diseased plants which are dying and guarantee the yield of oilseed rape and cruciferous crops, is stable in the treatment effect on the club root disease, does not easily cause environmental pollution, and has a field use effect that is not affected by geological conditions and soil environment, not only can the normal
2 Date Recue/Date Received 2020-05-15 absorption function of the roots of the diseased plants be rebuilt or restored, the crop growth be promoted, the yield be increased, the use of pesticides be significantly reduced, the stability of the farmland ecosystem be improved and the planting cost of cruciferous crops be reduced, but also the technical bottleneck that the club root diseased plants are difficult to survive can be overcome, the major practical production problem that it is difficult to treat the club root disease can be truly solved, the enthusiasm of farmers can be greatly stimulated and the sustainable and safe production of cruciferous crops can be promoted.
Summary of the Invention In order to overcome the disadvantages of the existing prevention and treatment technology and overcome the technical bottleneck that the club root diseased plants are difficult to survive, the present invention provides a growth promoter which can restore the absorption function of roots and can be used to prevent and treat club root disease of cruciferous crops.
The growth promoter comprises the following components in parts by weight: 0.3-0.5 part of indolebutyric acid, 0.3-0.5 part of naphthylacetic acid, 15-20 parts of humic acid, 10-20 parts of boron (based on element B) and 30-50 parts of plant nutrient. The technical purpose of the present invention is realized through the synergistic effect of the growth regulator (indolebutyric acid and naphthylacetic acid), humic acid, boron and plant nutrient, which are compounded according to appropriate components and proportions.
In the present invention, indolebutyric acid and naphthylacetic acid are agricultural available compounds. Humic acid is, for example, sodium humate or potassium humate. Boron is at least one of boric acid, borax and boron-magnesium fertilizer. The plant nutrient is a complexing agent of nitrogen, phosphorus and potassium, and the mass ratio is preferably N:P:K = 4:1:1.
Previous studies have showed that the infection process of the club root disease can be divided into two stages, i.e., the root hair infection stage and the cortex infection stage.
Summary of the Invention In order to overcome the disadvantages of the existing prevention and treatment technology and overcome the technical bottleneck that the club root diseased plants are difficult to survive, the present invention provides a growth promoter which can restore the absorption function of roots and can be used to prevent and treat club root disease of cruciferous crops.
The growth promoter comprises the following components in parts by weight: 0.3-0.5 part of indolebutyric acid, 0.3-0.5 part of naphthylacetic acid, 15-20 parts of humic acid, 10-20 parts of boron (based on element B) and 30-50 parts of plant nutrient. The technical purpose of the present invention is realized through the synergistic effect of the growth regulator (indolebutyric acid and naphthylacetic acid), humic acid, boron and plant nutrient, which are compounded according to appropriate components and proportions.
In the present invention, indolebutyric acid and naphthylacetic acid are agricultural available compounds. Humic acid is, for example, sodium humate or potassium humate. Boron is at least one of boric acid, borax and boron-magnesium fertilizer. The plant nutrient is a complexing agent of nitrogen, phosphorus and potassium, and the mass ratio is preferably N:P:K = 4:1:1.
Previous studies have showed that the infection process of the club root disease can be divided into two stages, i.e., the root hair infection stage and the cortex infection stage.
3 Date Recue/Date Received 2020-05-15 The root hair infection stage refers to the stage that doimant spores germinate and infect root hairs and epidermal cells of plants. The infection at this stage will not cause obvious symptoms to host plants, nor directly cause the yield or quality decline of the host plants. The cortex infection stage refers to the stage that the primary zoosporangia release secondary zoospores to infect cortex cells, which leads to the proliferation and hypertrophy of root tissue cells and the formation of swollen roots.
At this stage, the pathogens proliferate in a large scale in the host plants, and the root swelling seriously affects the water and nutrient absorption function of the plants till the plants are wilted and died. For the club root disease, if prevention and treatment with chemicals are adopted after symptoms appear, only a part of pathogens left in soil can be killed, but it has no effect on the pathogens in the host plants, and it cannot restore the normal water and nutrient absorption function of the roots, so the club root diseased plants are difficult to survive.
Indolebutyric acid, as an endogenous growth regulator, can promote cell division and cell growth, induce the formation of adventitious roots, increase fruit setting, prevent fruit abscission, and change the ratio of female flowers to male flowers.
Indolebutyric acid can enter plants through tender epidermis of leaves and branches and seeds, and is transported to an acting position together with the nutrient flow. At present, indolebutyric acid is mainly used for the rooting of cuttings, and can induce the formation of root primordium, promote cell differentiation and division, facilitate the formation of new roots and vascular systems, and promote the formation of adventitious roots of cuttings. Indolebutyric acid is generally used for root soaking in transplantation of woody and herbaceous plants, and it can also be used for seed soaking and seed dressing.
Naphthylacetic acid (NAA), a broad-spectrum plant growth regulator, can promote cell division and expansion, induce the formation of adventitious roots, increase fruit setting, prevent fruit abscission, and change the ratio of female flowers to male flowers. Naphthylacetic acid can enter the plants through the tender epidermis of leaves and branches and seeds, and is transported to the whole plants together with the
At this stage, the pathogens proliferate in a large scale in the host plants, and the root swelling seriously affects the water and nutrient absorption function of the plants till the plants are wilted and died. For the club root disease, if prevention and treatment with chemicals are adopted after symptoms appear, only a part of pathogens left in soil can be killed, but it has no effect on the pathogens in the host plants, and it cannot restore the normal water and nutrient absorption function of the roots, so the club root diseased plants are difficult to survive.
Indolebutyric acid, as an endogenous growth regulator, can promote cell division and cell growth, induce the formation of adventitious roots, increase fruit setting, prevent fruit abscission, and change the ratio of female flowers to male flowers.
Indolebutyric acid can enter plants through tender epidermis of leaves and branches and seeds, and is transported to an acting position together with the nutrient flow. At present, indolebutyric acid is mainly used for the rooting of cuttings, and can induce the formation of root primordium, promote cell differentiation and division, facilitate the formation of new roots and vascular systems, and promote the formation of adventitious roots of cuttings. Indolebutyric acid is generally used for root soaking in transplantation of woody and herbaceous plants, and it can also be used for seed soaking and seed dressing.
Naphthylacetic acid (NAA), a broad-spectrum plant growth regulator, can promote cell division and expansion, induce the formation of adventitious roots, increase fruit setting, prevent fruit abscission, and change the ratio of female flowers to male flowers. Naphthylacetic acid can enter the plants through the tender epidermis of leaves and branches and seeds, and is transported to the whole plants together with the
4 Date Recue/Date Received 2020-05-15 nutrient flow. Previous studies have shown that the use of a certain amount of NAA or sodium naphthalene in the plant growth process can significantly promote the plant growth and increase the yield. The results of treatment to papaya seeds by using different concentrations of NAA have showed that NAA increases the overall germination rate, germination potential and vigor index of the seeds, and can promote the photosynthesis of different plants.
Humic acid is a macromolecular organic substance widely existing in nature, which is widely used in agriculture, forestry, animal husbandry, chemical industry or the like.
In agricultural production, humic acid is mainly used to produce fertilizers (such as ammonium humate and potassium humate). The application of humic acid in soil can significantly improve the physical and chemical properties of the soil and enhance the fertilizer efficiency. Some studies have shown that the application of humic acid in soil can increase the content of microorganisms in soil, improve the nutrient utilization rate of crops, and significantly increase the biomass of sweet potato, grape, cabbage, tomato and other crops. Appropriate addition of humic acid can improve the root system activity of normally growing oilseed rape seedlings, significantly promote root system growth, and increase the activity of antioxidant enzymes in oilseed rape cells and the content of chlorophyll.
Boron is a microelement whose main function is to provide plant nutrients.
Conventional boron refers to boron chemical products mainly including borax, boric acid and boron-magnesium fertilizer, which are used as agricultural microelement fertilizers. Boron is one of the essential nutrients in plants. It is absorbed and utilized by plants in the form of boric acid molecule (H3B03), which is not easy to move in plants. Boron can promote the growth of the root system and play an important role in the synthesis and transport of photosynthesis products, i.e., carbohydrate.
The main role thereof is to have a special role in the normal process of fertilization.
Oilseed rape is very sensitive to boron, and lack of boron will lead to flowering but not fruiting, resulting in large-scale yield reduction. Studies have showed that boron application can significantly increase the number of pods per oilseed rape plant, the number of Date Recue/Date Received 2020-05-15 seeds per pod, the weight of a thousand seeds and the yield. However, improper application of boron easily causes boron poisoning to crops. Some scholars also have found that applying a certain proportion of calcium and boron fertilizers can control the occurrence of the club root disease.
The plant nutrient is to supplement various elements for plant growth to promote healthy plant growth. The main components of the plant nutrient in the present invention are nitrogen, phosphorus and potassium, which are the nutrient elements demanded by plants during growth and taken away during harvest in a large scale.
However, the amount of nitrogen, phosphorus and potassium returned to the soil in the form of residues and roots is not great, so it is often necessary to supplement these nutrients by applying fertilizers. Oilseed rape is a kind of crop that needs more fertilizers and has strong fertilizer tolerance. Nitrogen, phosphorus and potassium are necessary elements for the growth of oilseed rape. Results have showed that if only one, two or three of nitrogen, phosphorus, potassium and boron fertilizers are applied, it is difficult to significantly increase the yield of rapeseeds, and the reasonable combination and application of nitrogen, phosphorus, potassium and boron can significantly promote the growth and increase the yield of oilseed rape.
In the long-term study, the inventor has found that even if the affected root system infected with Plasmodiophora brassicae rots completely, it will not spread to and jeopardize the adjacent healthy root tissues, and only a few of the remaining healthy roots can continuously maintain the nutrient and water supply to the aboveground plants, and make the diseased plants be in a growing state. In combination with the theory of plant cell totipotency, it is speculated that the diseased plants can be induced to grow new root systems through external stimulation and the normal physiological function of the root system of the affected plants can be restored through rebuilding.
Through continuous years of study, it has shown that the traditional way that the root growth basically depends on application in soil is surmounted, and creatively through foliar spraying method and the growth promoter formula of the present invention, the healthy growth of the diseased plants is completely restored and the physiological Date Recue/Date Received 2020-05-15 function of the root systems of the diseased plants is rebuilt. The special treatment method of performing foliar treatment to the club root diseased plants restores the vitality of the affected plants. The application of this technology in the field can significantly reduce the loss caused by the disease, make the survival rate of the diseased plants reach more than 90%, and increase the yield by more than 60%.
The growth promoter in the present invention combines indolebutyric acid, naphthylacetic acid, humic acid, boron and plant growth agent according to appropriate components and proportions based on the principle of the synergistic effect of all components, can rebuild the water and nutrient absorption function of the root system of the club root diseased plants by selecting the special sensitive period of the plants, and restore the normal growth of the diseased plants which are dying. The growth promoter is applied by adopting a foliar spraying method at the early bolting stage of oilseed rape , when the field soil temperature is higher than the atmospheric temperature, or at the moment that the club root diseased plants of the cruciferous crops still have 1/5 normal roots. Compared with the traditional way of application at roots (it is required to be used within specific time, the duration of which cannot exceed 3 days), the use time range is wider, the operation is simpler and the practicability is stronger.
Preferably, the amount of used indolebutyric acid is 0.3-0.5g/mu, and the amount of used water is 40-50L/667m2. Correspondingly, the amount of used naphthylacetic acid is 0.3-0.5g/667m2; the amount of used humic acid is 15-20g/667m2; the amount of used boron is 10-20g/667m2; and the amount of used plant nutrient is 30-50g/667m2.
Compared with application at roots in the prior art, the amounts of the used chemicals are greatly reduced and the costs of the chemicals are also greatly reduced.
The present invention provides a growth promoter for restoring the root absorption function and preventing and treating club root diseases of cruciferous crops.
On the one hand, it can meet the requirements on nutrients for the growth of cruciferous crops such as oilseed rape. On the other hand, it can induce the root growth through exogenous stimulation, rebuild the normal physiological function of the root system Date Recue/Date Received 2020-05-15 of the diseased plants and restore the vitality of the diseased plants which are dying. It is applicable to the prevention and treatment of the club root disease of the cruciferous crops such as oilseed rape and cabbage.
Compared with the prior art, the present invention has the following advantages:
firstly, the usage amount is small, the effect is remarkable, the benefit is high, the application objects are more and the application scope is wide; secondly, the normal growth of the diseased plants which are dying can be restored through the special way of rebuilding the water and nutrient absorption function of the root system of the diseased plants, so that the survival rate of the diseased plants can reach more than 90%, and the yield can be increased by more than 60%, the yield of oilseed rape and other cruciferous crops is guaranteed, and the treatment effect on the club root disease is outstanding and stable; thirdly, the technical bottleneck that the club root diseased plants are difficult to survive and treat is overcome, it is free of chemical pesticides, environment-friendly, and convenient and quick to use, and compared with traditional root prevention and treatment method, the costs of chemicals and labor are reduced by more than 80%; and fourthly, the effect of foliar application is not affected by geological conditions and soil environment, the potential risks of food safety and environmental pollution caused by the application of chemical pesticides are reduced in a large scale, and the social, economic and ecological benefits are remarkable.
Description of the Examples The present invention will be further described below in combination with the examples.
Example 1 A growth promoter comprised the following components based on the amount of effective components per 667m2 of oilseed rape:
Indolebutyric acid 0.5g;
Naphthylacetic acid 0.5g;
Date Recue/Date Received 2020-05-15 Humic acid 20g;
Boron element (borax B) 20g;
Plant nutrient (N:P:K = 3:2:1) 50g.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Example 2 A growth promoter comprised the following components based on the amount of effective components per mu of oilseed rape:
Indolebutyric acid 0.3g;
Naphthylacetic acid 0.3g;
Humic acid 15g;
Boron element (borax B) 10g;
Plant nutrient (N:P:K = 3:2:1) 30g.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Example 3 A growth promoter comprised the following components based on the amount of effective components per 667m2 of oilseed rape:
Indolebutyric acid 0.4g;
Naphthylacetic acid 0.4g;
Humic acid 16g;
Boron element (borax B) 15g;
Plant nutrient (N:P:K = 4:1:1) 40g.
Date Recue/Date Received 2020-05-15 When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Example 4 A growth promoter comprised the following components based on the amount of effective components per 667m2 of oilseed rape:
Indolebutyric acid 0.3g;
Naphthylacetic acid 0.5g;
Humic acid 20g;
Boron element (borax B) 10g;
Plant nutrient (N:P:K = 3:2:1) 45g.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Example 5 A growth promoter comprised the following components based on the amount of effective components per 667m2 of oilseed rape:
Indolebutyric acid 0.5g;
Naphthylacetic acid 0.3g;
Humic acid 15g;
Boron element (borax B) 20g;
Plant nutrient (N:P:K = 3:2:1) 30g.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Comparative example 1 A growth promoter only comprised 16g of humic acid.
Date Recue/Date Received 2020-05-15 When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Comparative example 2 A growth promoter only comprised 0.4g of indolebutyric acid.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Comparative example 3 A growth promoter only comprised 0.4g of naphthylacetic acid.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Comparative example 4 A growth promoter only comprised 0.4g of boron element.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Comparative example 5 No chemical was added in this comparative example and only clean water was sprayed.
Experimental verification In November 2017, in Xigao Town, Guanghan, 30 rape (at the 13-leaves stage) fields with high incidence of club root disease (the area of each field was 667m2) were selected for parallel test. The selection standard of test fields was that more than 80%
of oilseed rape plants were infected with the club root disease.
The treatment method was as follows: three test fields were randomly selected to be sprayed with the water/solution in examples 1-5 and comparative examples 1-5 by means of foliar spraying, i.e., the chemical in example 1 was sprayed to three fields, Date Recue/Date Received 2020-05-15 and so on. The test results were averaged.
See Table 1 for the test results.
Table 1 Test results Number of Number of Increase of plant Increase of yield per diseased surviving height (cm) 667m2 (kg) plants plants Example 1 6510 5530 1.5 51 Example 2 6460 5550 1.6 53 Example 3 6430 5790 2.1 65 Example 4 6550 5560 1.7 55 Example 5 6390 5560 1.6 57 Comparative 6400 2300 0.3 15 example 1 Comparative 6510 2130 0.5 13 example 2 Comparative 6450 1960 0.3 16 example 3 Comparative 6490 1190 0.2 9 example 4 Comparative example 5 The above examples are only preferred examples of the present invention and are not used for limiting the present invention. Any modification, equivalent replacement and improvement made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Date Recue/Date Received 2020-05-15
Humic acid is a macromolecular organic substance widely existing in nature, which is widely used in agriculture, forestry, animal husbandry, chemical industry or the like.
In agricultural production, humic acid is mainly used to produce fertilizers (such as ammonium humate and potassium humate). The application of humic acid in soil can significantly improve the physical and chemical properties of the soil and enhance the fertilizer efficiency. Some studies have shown that the application of humic acid in soil can increase the content of microorganisms in soil, improve the nutrient utilization rate of crops, and significantly increase the biomass of sweet potato, grape, cabbage, tomato and other crops. Appropriate addition of humic acid can improve the root system activity of normally growing oilseed rape seedlings, significantly promote root system growth, and increase the activity of antioxidant enzymes in oilseed rape cells and the content of chlorophyll.
Boron is a microelement whose main function is to provide plant nutrients.
Conventional boron refers to boron chemical products mainly including borax, boric acid and boron-magnesium fertilizer, which are used as agricultural microelement fertilizers. Boron is one of the essential nutrients in plants. It is absorbed and utilized by plants in the form of boric acid molecule (H3B03), which is not easy to move in plants. Boron can promote the growth of the root system and play an important role in the synthesis and transport of photosynthesis products, i.e., carbohydrate.
The main role thereof is to have a special role in the normal process of fertilization.
Oilseed rape is very sensitive to boron, and lack of boron will lead to flowering but not fruiting, resulting in large-scale yield reduction. Studies have showed that boron application can significantly increase the number of pods per oilseed rape plant, the number of Date Recue/Date Received 2020-05-15 seeds per pod, the weight of a thousand seeds and the yield. However, improper application of boron easily causes boron poisoning to crops. Some scholars also have found that applying a certain proportion of calcium and boron fertilizers can control the occurrence of the club root disease.
The plant nutrient is to supplement various elements for plant growth to promote healthy plant growth. The main components of the plant nutrient in the present invention are nitrogen, phosphorus and potassium, which are the nutrient elements demanded by plants during growth and taken away during harvest in a large scale.
However, the amount of nitrogen, phosphorus and potassium returned to the soil in the form of residues and roots is not great, so it is often necessary to supplement these nutrients by applying fertilizers. Oilseed rape is a kind of crop that needs more fertilizers and has strong fertilizer tolerance. Nitrogen, phosphorus and potassium are necessary elements for the growth of oilseed rape. Results have showed that if only one, two or three of nitrogen, phosphorus, potassium and boron fertilizers are applied, it is difficult to significantly increase the yield of rapeseeds, and the reasonable combination and application of nitrogen, phosphorus, potassium and boron can significantly promote the growth and increase the yield of oilseed rape.
In the long-term study, the inventor has found that even if the affected root system infected with Plasmodiophora brassicae rots completely, it will not spread to and jeopardize the adjacent healthy root tissues, and only a few of the remaining healthy roots can continuously maintain the nutrient and water supply to the aboveground plants, and make the diseased plants be in a growing state. In combination with the theory of plant cell totipotency, it is speculated that the diseased plants can be induced to grow new root systems through external stimulation and the normal physiological function of the root system of the affected plants can be restored through rebuilding.
Through continuous years of study, it has shown that the traditional way that the root growth basically depends on application in soil is surmounted, and creatively through foliar spraying method and the growth promoter formula of the present invention, the healthy growth of the diseased plants is completely restored and the physiological Date Recue/Date Received 2020-05-15 function of the root systems of the diseased plants is rebuilt. The special treatment method of performing foliar treatment to the club root diseased plants restores the vitality of the affected plants. The application of this technology in the field can significantly reduce the loss caused by the disease, make the survival rate of the diseased plants reach more than 90%, and increase the yield by more than 60%.
The growth promoter in the present invention combines indolebutyric acid, naphthylacetic acid, humic acid, boron and plant growth agent according to appropriate components and proportions based on the principle of the synergistic effect of all components, can rebuild the water and nutrient absorption function of the root system of the club root diseased plants by selecting the special sensitive period of the plants, and restore the normal growth of the diseased plants which are dying. The growth promoter is applied by adopting a foliar spraying method at the early bolting stage of oilseed rape , when the field soil temperature is higher than the atmospheric temperature, or at the moment that the club root diseased plants of the cruciferous crops still have 1/5 normal roots. Compared with the traditional way of application at roots (it is required to be used within specific time, the duration of which cannot exceed 3 days), the use time range is wider, the operation is simpler and the practicability is stronger.
Preferably, the amount of used indolebutyric acid is 0.3-0.5g/mu, and the amount of used water is 40-50L/667m2. Correspondingly, the amount of used naphthylacetic acid is 0.3-0.5g/667m2; the amount of used humic acid is 15-20g/667m2; the amount of used boron is 10-20g/667m2; and the amount of used plant nutrient is 30-50g/667m2.
Compared with application at roots in the prior art, the amounts of the used chemicals are greatly reduced and the costs of the chemicals are also greatly reduced.
The present invention provides a growth promoter for restoring the root absorption function and preventing and treating club root diseases of cruciferous crops.
On the one hand, it can meet the requirements on nutrients for the growth of cruciferous crops such as oilseed rape. On the other hand, it can induce the root growth through exogenous stimulation, rebuild the normal physiological function of the root system Date Recue/Date Received 2020-05-15 of the diseased plants and restore the vitality of the diseased plants which are dying. It is applicable to the prevention and treatment of the club root disease of the cruciferous crops such as oilseed rape and cabbage.
Compared with the prior art, the present invention has the following advantages:
firstly, the usage amount is small, the effect is remarkable, the benefit is high, the application objects are more and the application scope is wide; secondly, the normal growth of the diseased plants which are dying can be restored through the special way of rebuilding the water and nutrient absorption function of the root system of the diseased plants, so that the survival rate of the diseased plants can reach more than 90%, and the yield can be increased by more than 60%, the yield of oilseed rape and other cruciferous crops is guaranteed, and the treatment effect on the club root disease is outstanding and stable; thirdly, the technical bottleneck that the club root diseased plants are difficult to survive and treat is overcome, it is free of chemical pesticides, environment-friendly, and convenient and quick to use, and compared with traditional root prevention and treatment method, the costs of chemicals and labor are reduced by more than 80%; and fourthly, the effect of foliar application is not affected by geological conditions and soil environment, the potential risks of food safety and environmental pollution caused by the application of chemical pesticides are reduced in a large scale, and the social, economic and ecological benefits are remarkable.
Description of the Examples The present invention will be further described below in combination with the examples.
Example 1 A growth promoter comprised the following components based on the amount of effective components per 667m2 of oilseed rape:
Indolebutyric acid 0.5g;
Naphthylacetic acid 0.5g;
Date Recue/Date Received 2020-05-15 Humic acid 20g;
Boron element (borax B) 20g;
Plant nutrient (N:P:K = 3:2:1) 50g.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Example 2 A growth promoter comprised the following components based on the amount of effective components per mu of oilseed rape:
Indolebutyric acid 0.3g;
Naphthylacetic acid 0.3g;
Humic acid 15g;
Boron element (borax B) 10g;
Plant nutrient (N:P:K = 3:2:1) 30g.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Example 3 A growth promoter comprised the following components based on the amount of effective components per 667m2 of oilseed rape:
Indolebutyric acid 0.4g;
Naphthylacetic acid 0.4g;
Humic acid 16g;
Boron element (borax B) 15g;
Plant nutrient (N:P:K = 4:1:1) 40g.
Date Recue/Date Received 2020-05-15 When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Example 4 A growth promoter comprised the following components based on the amount of effective components per 667m2 of oilseed rape:
Indolebutyric acid 0.3g;
Naphthylacetic acid 0.5g;
Humic acid 20g;
Boron element (borax B) 10g;
Plant nutrient (N:P:K = 3:2:1) 45g.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Example 5 A growth promoter comprised the following components based on the amount of effective components per 667m2 of oilseed rape:
Indolebutyric acid 0.5g;
Naphthylacetic acid 0.3g;
Humic acid 15g;
Boron element (borax B) 20g;
Plant nutrient (N:P:K = 3:2:1) 30g.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Comparative example 1 A growth promoter only comprised 16g of humic acid.
Date Recue/Date Received 2020-05-15 When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Comparative example 2 A growth promoter only comprised 0.4g of indolebutyric acid.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Comparative example 3 A growth promoter only comprised 0.4g of naphthylacetic acid.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Comparative example 4 A growth promoter only comprised 0.4g of boron element.
When applied, the above growth promoter was mixed with 50kg of water for full dissolution and then the solution was sprayed for 667m2.
Comparative example 5 No chemical was added in this comparative example and only clean water was sprayed.
Experimental verification In November 2017, in Xigao Town, Guanghan, 30 rape (at the 13-leaves stage) fields with high incidence of club root disease (the area of each field was 667m2) were selected for parallel test. The selection standard of test fields was that more than 80%
of oilseed rape plants were infected with the club root disease.
The treatment method was as follows: three test fields were randomly selected to be sprayed with the water/solution in examples 1-5 and comparative examples 1-5 by means of foliar spraying, i.e., the chemical in example 1 was sprayed to three fields, Date Recue/Date Received 2020-05-15 and so on. The test results were averaged.
See Table 1 for the test results.
Table 1 Test results Number of Number of Increase of plant Increase of yield per diseased surviving height (cm) 667m2 (kg) plants plants Example 1 6510 5530 1.5 51 Example 2 6460 5550 1.6 53 Example 3 6430 5790 2.1 65 Example 4 6550 5560 1.7 55 Example 5 6390 5560 1.6 57 Comparative 6400 2300 0.3 15 example 1 Comparative 6510 2130 0.5 13 example 2 Comparative 6450 1960 0.3 16 example 3 Comparative 6490 1190 0.2 9 example 4 Comparative example 5 The above examples are only preferred examples of the present invention and are not used for limiting the present invention. Any modification, equivalent replacement and improvement made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.
Date Recue/Date Received 2020-05-15
Claims (6)
1. A growth promoter for use in the treatment of clubroot disease of oilseed rape, wherein the growth promoter comprises the following components in parts by weight:
0.3-0.5 part of indolebutyric acid, 0.3-0.5 part of naphthylacetic acid, 15-20 parts of humic acid, 10-20 parts of boron and 30-50 parts of plant nutrient; and wherein the growth promoter is applied by adopting a foliar spraying method at an early bolting stage of the oilseed rape, when a field soil temperature is higher than an atmospheric temperature, or when clubroot diseased plants of the oilseed rape still have 1/5 normal roots.
0.3-0.5 part of indolebutyric acid, 0.3-0.5 part of naphthylacetic acid, 15-20 parts of humic acid, 10-20 parts of boron and 30-50 parts of plant nutrient; and wherein the growth promoter is applied by adopting a foliar spraying method at an early bolting stage of the oilseed rape, when a field soil temperature is higher than an atmospheric temperature, or when clubroot diseased plants of the oilseed rape still have 1/5 normal roots.
2. The growth promoter for use according to claim 1, wherein the humic acid is sodium humate and/or potassium humate.
3. The growth promoter for use according to claim 1, wherein the boron is at least one of boric acid, borax and boron-magnesium fertilizer.
4. The growth promoter for use according to claim 1, wherein the plant nutrient is a complexing agent of nitrogen, phosphorus and potassium.
5. The gowth promoter for use according to claim 4, wherein the mass ratio of nitrogen to phosphorus to potassium is N:P:K = 4:1:1.
6. The growth promoter for use according to claim 1, wherein in the foliar spraying method, the indolebutyric acid is applied at an amount of 0.3-0.5g/667m2 and water is added at an amount of 40-50L/667m2.
Date Reçue/Date Received 2022-10-21
Date Reçue/Date Received 2022-10-21
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| PCT/CN2019/078345 WO2020186400A1 (en) | 2019-03-15 | 2019-03-15 | Growth promoter and use thereof in preventing and treating clubroot disease in cruciferous crop |
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| CN103694045B (en) * | 2013-12-18 | 2015-09-09 | 徐跃华 | A kind ofly to supplement the nutrients for synergistic and the compound formulation and its preparation method and application of disease-resistant yield-increasing |
| CN104003811B (en) * | 2014-06-16 | 2018-03-16 | 四川省农业科学院植物保护研究所 | Prevent and treat root-growing agent, preparation method and the purposes of crop in cruciferae clubroot |
| CN107686405A (en) * | 2017-09-20 | 2018-02-13 | 荆门市东栗农特技术服务中心 | One kind preventing and treating rapeseed clubroot liquid fertilizer |
| CN108530186A (en) * | 2018-06-15 | 2018-09-14 | 山东罗纳朗克生物农化有限公司 | It is a kind of to pass root disease with the medicine fertilizer for stimulating plant growth effect for preventing soil |
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