CA3042854A1 - Cellules hotes recombinantes et procedes de production anaerobie de l-aspartate et de beta-alanine - Google Patents

Cellules hotes recombinantes et procedes de production anaerobie de l-aspartate et de beta-alanine Download PDF

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Publication number
CA3042854A1
CA3042854A1 CA3042854A CA3042854A CA3042854A1 CA 3042854 A1 CA3042854 A1 CA 3042854A1 CA 3042854 A CA3042854 A CA 3042854A CA 3042854 A CA3042854 A CA 3042854A CA 3042854 A1 CA3042854 A1 CA 3042854A1
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Canada
Prior art keywords
aspartate
seq
beta
alanine
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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CA3042854A
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English (en)
Inventor
Jeffrey Dietrich
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lygos Inc
Original Assignee
Lygos Inc
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Filing date
Publication date
Application filed by Lygos Inc filed Critical Lygos Inc
Publication of CA3042854A1 publication Critical patent/CA3042854A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0014—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4)
    • C12N9/0016—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on the CH-NH2 group of donors (1.4) with NAD or NADP as acceptor (1.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/20—Aspartic acid; Asparagine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y104/00—Oxidoreductases acting on the CH-NH2 group of donors (1.4)
    • C12Y104/01—Oxidoreductases acting on the CH-NH2 group of donors (1.4) with NAD+ or NADP+ as acceptor (1.4.1)
    • C12Y104/01021—Aspartate dehydrogenase (1.4.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)
    • C12Y401/01011—Aspartate 1-decarboxylase (4.1.1.11)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y604/00—Ligases forming carbon-carbon bonds (6.4)
    • C12Y604/01—Ligases forming carbon-carbon bonds (6.4.1)
    • C12Y604/01001—Pyruvate carboxylase (6.4.1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des cellules hôtes recombinantes, des matériaux et des procédés pour la production biologique de L-aspartate et/ou de bêta-alanine dans des conditions sensiblement anaérobies.
CA3042854A 2015-11-12 2016-11-11 Cellules hotes recombinantes et procedes de production anaerobie de l-aspartate et de beta-alanine Abandoned CA3042854A1 (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562254635P 2015-11-12 2015-11-12
US62/254,635 2015-11-12
PCT/US2016/061578 WO2017083683A1 (fr) 2015-11-12 2016-11-11 Cellules hôtes recombinantes et procédés de production anaérobie de l-aspartate et de bêta-alanine

Publications (1)

Publication Number Publication Date
CA3042854A1 true CA3042854A1 (fr) 2017-05-18

Family

ID=58696123

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3042854A Abandoned CA3042854A1 (fr) 2015-11-12 2016-11-11 Cellules hotes recombinantes et procedes de production anaerobie de l-aspartate et de beta-alanine

Country Status (5)

Country Link
EP (1) EP3374505A4 (fr)
CN (1) CN109072172A (fr)
AU (1) AU2016353242A1 (fr)
CA (1) CA3042854A1 (fr)
WO (1) WO2017083683A1 (fr)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109593748B (zh) 2017-10-01 2022-03-04 宁波酶赛生物工程有限公司 工程化脱羧酶多肽及其在制备β-丙氨酸中的应用
CN113039281A (zh) * 2018-06-25 2021-06-25 利戈斯股份有限公司 生产天冬氨酸和β-丙氨酸的重组宿主细胞和方法
CN109735522B (zh) * 2018-12-26 2021-02-02 浙江工业大学 一种L-天冬氨酸-α-脱羧酶突变体及其应用
CN111778200B (zh) * 2019-04-04 2022-11-01 中国科学院微生物研究所 生产L-天冬氨酸的平台菌、基于该平台菌构建的生产β-丙氨酸的重组菌及其构建方法
CN115916980A (zh) 2020-06-17 2023-04-04 住友化学株式会社 生产天冬氨酸和甲硫氨酸的嗜氢菌属细菌的转化体
CN112410245B (zh) * 2020-10-13 2024-03-08 哈尔滨雁成生物科技有限公司 一种低温堆肥微生物复合菌剂及其制备方法和应用
CN113604459B (zh) * 2021-08-31 2023-10-10 江南大学 一种磷酸烯醇式丙酮酸羧激酶及其应用

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8048624B1 (en) * 2007-12-04 2011-11-01 Opx Biotechnologies, Inc. Compositions and methods for 3-hydroxypropionate bio-production from biomass
US20100021978A1 (en) * 2008-07-23 2010-01-28 Genomatica, Inc. Methods and organisms for production of 3-hydroxypropionic acid
WO2011041426A1 (fr) * 2009-09-29 2011-04-07 Butamax(Tm) Advanced Biofuels Llc Cellules hôtes à production améliorée de levure
CA2777459A1 (fr) * 2009-10-13 2011-04-21 Genomatica, Inc. Microorganismes pour la production de 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-coa, putrescine et de composes associes, et procedes afferents
RU2010101135A (ru) * 2010-01-15 2011-07-20 Закрытое акционерное общество "Научно-исследовательский институт "Аджиномото-Генетика" (ЗАО АГРИ) (RU) Бактерия семейства enterobacteriaceae - продуцент l-аспартата или метаболитов, производных l-аспартата, и способ получения l-аспартата или метаболитов, производных l-аспартата
WO2012019175A2 (fr) * 2010-08-06 2012-02-09 Mascoma Corporation Production de produits dérivés du malonyl-coa par voie anaérobie
WO2012074818A2 (fr) * 2010-11-22 2012-06-07 Novozymes, Inc. Compositions et procédés pour la production d'acide 3-hydroxypropionique
ES2698500T3 (es) * 2012-03-06 2019-02-05 Lygos Inc Células huésped recombinantes para la producción de malonato
WO2015031504A1 (fr) * 2013-08-27 2015-03-05 The Regents Of The University Of California Voie et organismes recombinés pour la synthèse de la malonyl-coenzyme a

Also Published As

Publication number Publication date
EP3374505A4 (fr) 2019-05-01
WO2017083683A1 (fr) 2017-05-18
AU2016353242A1 (en) 2018-06-21
CN109072172A (zh) 2018-12-21
EP3374505A1 (fr) 2018-09-19

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FZDE Discontinued

Effective date: 20220512

FZDE Discontinued

Effective date: 20220512