CA3035286A1 - Method and analysis system for testing a sample - Google Patents
Method and analysis system for testing a sample Download PDFInfo
- Publication number
- CA3035286A1 CA3035286A1 CA3035286A CA3035286A CA3035286A1 CA 3035286 A1 CA3035286 A1 CA 3035286A1 CA 3035286 A CA3035286 A CA 3035286A CA 3035286 A CA3035286 A CA 3035286A CA 3035286 A1 CA3035286 A1 CA 3035286A1
- Authority
- CA
- Canada
- Prior art keywords
- temperature
- amplification products
- sensor
- group
- different
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
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- ZYPZVOKVDNSKLP-UHFFFAOYSA-N tris(4-aminophenyl) phosphate Chemical compound C1=CC(N)=CC=C1OP(=O)(OC=1C=CC(N)=CC=1)OC1=CC=C(N)C=C1 ZYPZVOKVDNSKLP-UHFFFAOYSA-N 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- ITZMJCSORYKOSI-AJNGGQMLSA-N APGPR Enterostatin Chemical compound C[C@H](N)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N1[C@H](C(=O)N[C@@H](CCCN=C(N)N)C(O)=O)CCC1 ITZMJCSORYKOSI-AJNGGQMLSA-N 0.000 description 1
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- 238000007599 discharging Methods 0.000 description 1
- 238000000835 electrochemical detection Methods 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
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- 229910000510 noble metal Inorganic materials 0.000 description 1
- 238000007826 nucleic acid assay Methods 0.000 description 1
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- 229920003023 plastic Polymers 0.000 description 1
- 229910052697 platinum Inorganic materials 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
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- 238000002731 protein assay Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
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- 239000011535 reaction buffer Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 229960001790 sodium citrate Drugs 0.000 description 1
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- 150000003431 steroids Chemical class 0.000 description 1
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- 239000001226 triphosphate Substances 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6825—Nucleic acid detection involving sensors
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- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
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- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
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- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/00029—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor provided with flat sample substrates, e.g. slides
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- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0605—Metering of fluids
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- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/10—Integrating sample preparation and analysis in single entity, e.g. lab-on-a-chip concept
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- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0636—Integrated biosensor, microarrays
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- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0645—Electrodes
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- B01L2300/00—Additional constructional details
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- B01L2300/0627—Sensor or part of a sensor is integrated
- B01L2300/0663—Whole sensors
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- B01L2300/08—Geometry, shape and general structure
- B01L2300/0809—Geometry, shape and general structure rectangular shaped
- B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
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- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
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- B01L2300/18—Means for temperature control
- B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
- B01L2300/1822—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using Peltier elements
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- B—PERFORMING OPERATIONS; TRANSPORTING
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- B01L2400/04—Moving fluids with specific forces or mechanical means
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- B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/101—Temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/519—Detection characterised by immobilisation to a surface characterised by the capture moiety being a single stranded oligonucleotide
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/607—Detection means characterised by use of a special device being a sensor, e.g. electrode
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/60—Detection means characterised by use of a special device
- C12Q2565/629—Detection means characterised by use of a special device being a microfluidic device
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N2035/00465—Separating and mixing arrangements
- G01N2035/00564—Handling or washing solid phase elements, e.g. beads
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Fluid Mechanics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP16020370 | 2016-10-07 | ||
| EP16020370.9 | 2016-10-07 | ||
| PCT/EP2017/025278 WO2018065101A1 (en) | 2016-10-07 | 2017-10-05 | Method and analysis system for testing a sample |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3035286A1 true CA3035286A1 (en) | 2018-04-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3035286A Abandoned CA3035286A1 (en) | 2016-10-07 | 2017-10-05 | Method and analysis system for testing a sample |
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| JP (1) | JP6987133B2 (enExample) |
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| WO (1) | WO2018065101A1 (enExample) |
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| KR20190066619A (ko) * | 2016-10-07 | 2019-06-13 | 베링거잉겔하임베트메디카게엠베하 | 샘플을 테스트하기 위한 방법 및 분석 시스템 |
| WO2022136243A1 (en) * | 2020-12-21 | 2022-06-30 | Boehringer Ingelheim Vetmedica Gmbh | Cartridge and analysis system for testing a sample |
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| US5096669A (en) | 1988-09-15 | 1992-03-17 | I-Stat Corporation | Disposable sensing device for real time fluid analysis |
| US6942771B1 (en) | 1999-04-21 | 2005-09-13 | Clinical Micro Sensors, Inc. | Microfluidic systems in the electrochemical detection of target analytes |
| AT410444B (de) * | 2001-03-02 | 2003-04-25 | Oesterr Forsch Seibersdorf | Verfahren zur detektion von nukleinsäuremolekülen |
| DE10111457B4 (de) | 2001-03-09 | 2006-12-14 | Siemens Ag | Diagnoseeinrichtung |
| EP1439910A2 (en) | 2001-07-26 | 2004-07-28 | Motorola, Inc. | System and methods for mixing within a microfluidic device |
| EP1636599B1 (de) | 2003-05-13 | 2007-05-02 | Siemens Aktiengesellschaft | Schaltkreisanordnung zur potentialkonstanthaltung an einem biosensor und zur digitalisierung des messstroms |
| ES2871885T3 (es) | 2005-05-25 | 2021-11-02 | Boehringer Ingelheim Vetmedica Gmbh | Sistema para el análisis integrado y automatizado de ADN o proteínas y procedimientos operativos de dicho sistema |
| DE102005059535B4 (de) | 2005-12-13 | 2010-01-14 | Siemens Ag | Vorrichtung und Verfahren zur Durchführung eines Nukleinsäure-Tests, sowie Verfahren zur Herstellung einer solchen Vorrichtung |
| GB2436616A (en) | 2006-03-29 | 2007-10-03 | Inverness Medical Switzerland | Assay device and method |
| WO2008147382A1 (en) * | 2006-09-27 | 2008-12-04 | Micronics, Inc. | Integrated microfluidic assay devices and methods |
| EP2384432B1 (en) * | 2007-06-21 | 2016-12-28 | Gen-Probe Incorporated | Instrument and receptacles for performing processes |
| EP1947197A1 (en) | 2007-08-24 | 2008-07-23 | Siemens Aktiengesellschaft, A German Corporation | Method for the amplification of at least one nucleic acid |
| US8741815B2 (en) | 2008-02-19 | 2014-06-03 | Intelligent Bio Systems, Inc. | Methods and devices for amplification of nucleic acid |
| WO2011063408A1 (en) * | 2009-11-23 | 2011-05-26 | Cyvek, Inc. | Method and apparatus for performing assays |
| US9651568B2 (en) | 2009-11-23 | 2017-05-16 | Cyvek, Inc. | Methods and systems for epi-fluorescent monitoring and scanning for microfluidic assays |
| DE102011015184B4 (de) | 2010-06-02 | 2013-11-21 | Thinxxs Microtechnology Ag | Vorrichtung für den Transport kleiner Volumina eines Fluids, insbesondere Mikropumpe oder Mikroventil |
| US20120157332A1 (en) | 2010-10-14 | 2012-06-21 | Meso Scale Technologies, Llc | Reagent Storage in an Assay Device |
| CA2830533C (en) * | 2011-03-22 | 2020-02-18 | Cyvek, Inc. | Microfluidic devices and methods of manufacture and use |
| US8614087B2 (en) | 2011-05-02 | 2013-12-24 | Ibis Biosciences, Inc. | Multiple-analyte assay device and system |
| US20130067525A1 (en) | 2011-09-09 | 2013-03-14 | Pct International, Inc. | Service provisioning device with integrated cable modem |
| US20130203057A1 (en) * | 2012-01-20 | 2013-08-08 | Biohelix Corporation | Step-wise detection of multiple target sequences in isothermal nucleic acid amplification reactions |
| WO2013161964A1 (ja) | 2012-04-27 | 2013-10-31 | キリンホールディングス株式会社 | リガンドを高感度に検出する核酸分子並びに該核酸分子のスクリーニング方法および該核酸分子の感度の最適化方法 |
| US9217179B2 (en) | 2012-05-24 | 2015-12-22 | The Governing Council Of The University Of Toronto | Systems and methods for multiplexed electrochemical detection |
| US9624532B2 (en) | 2013-02-05 | 2017-04-18 | Neil Gordon | Ultra-sensitive detection of extremely low level biological analytes using electrochemical signal amplification and biosensor |
| WO2015002975A1 (en) * | 2013-07-05 | 2015-01-08 | University Of Washington Through Its Center For Commercialization | Methods, compositions and systems for microfluidic assays |
| US20160223536A1 (en) | 2013-10-10 | 2016-08-04 | Song Diagnostic Research Llc | Improved Lateral Flow Assays |
| GB201320071D0 (en) | 2013-11-13 | 2013-12-25 | Univ Leuven Kath | Monitoring multiplex DNA amplification |
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- 2017-10-05 AU AU2017340652A patent/AU2017340652A1/en not_active Abandoned
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- 2017-10-05 JP JP2019518512A patent/JP6987133B2/ja not_active Expired - Fee Related
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Also Published As
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| MX385383B (es) | 2025-03-11 |
| ES2875790T3 (es) | 2021-11-11 |
| US20180100187A1 (en) | 2018-04-12 |
| KR20190066039A (ko) | 2019-06-12 |
| PL3523448T3 (pl) | 2021-10-18 |
| JP2019532647A (ja) | 2019-11-14 |
| TWI760373B (zh) | 2022-04-11 |
| JP6987133B2 (ja) | 2021-12-22 |
| WO2018065101A1 (en) | 2018-04-12 |
| NZ752615A (en) | 2021-07-30 |
| EP3523448A1 (en) | 2019-08-14 |
| AU2017340652A1 (en) | 2019-05-02 |
| EP3523448B1 (en) | 2021-03-31 |
| DK3523448T3 (da) | 2021-06-28 |
| BR112019006847A2 (pt) | 2019-06-25 |
| CN109790571A (zh) | 2019-05-21 |
| TW201821798A (zh) | 2018-06-16 |
| US10604792B2 (en) | 2020-03-31 |
| MX2019003926A (es) | 2019-06-10 |
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| EEER | Examination request |
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| FZDE | Discontinued |
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