CA3030704A1 - Biological nanopores for biopolymer sensing and sequencing based on frac actinoporin - Google Patents

Biological nanopores for biopolymer sensing and sequencing based on frac actinoporin Download PDF

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Publication number
CA3030704A1
CA3030704A1 CA3030704A CA3030704A CA3030704A1 CA 3030704 A1 CA3030704 A1 CA 3030704A1 CA 3030704 A CA3030704 A CA 3030704A CA 3030704 A CA3030704 A CA 3030704A CA 3030704 A1 CA3030704 A1 CA 3030704A1
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Prior art keywords
frac
nanopore
mutant
analyte
pore
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CA3030704A
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English (en)
French (fr)
Inventor
Giovanni Maglia
Carsten WLOKA
Natalie Lisa MUTTER
Misha SOSKINE
Gang Huang
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Rijksuniversiteit Groningen
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Rijksuniversiteit Groningen
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44791—Microapparatus
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B82—NANOTECHNOLOGY
    • B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Nanotechnology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Dispersion Chemistry (AREA)
  • Electrochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3030704A 2016-07-12 2017-05-24 Biological nanopores for biopolymer sensing and sequencing based on frac actinoporin Pending CA3030704A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662360999P 2016-07-12 2016-07-12
US62/360,999 2016-07-12
PCT/NL2017/050331 WO2018012963A1 (en) 2016-07-12 2017-05-24 Biological nanopores for biopolymer sensing and sequencing based on frac actinoporin

Publications (1)

Publication Number Publication Date
CA3030704A1 true CA3030704A1 (en) 2018-01-18

Family

ID=59270080

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3030704A Pending CA3030704A1 (en) 2016-07-12 2017-05-24 Biological nanopores for biopolymer sensing and sequencing based on frac actinoporin

Country Status (8)

Country Link
US (2) US11312755B2 (https=)
EP (2) EP3828280A1 (https=)
JP (4) JP7019665B2 (https=)
CN (2) CN109890980A (https=)
AU (3) AU2017295442B2 (https=)
CA (1) CA3030704A1 (https=)
ES (1) ES2843099T3 (https=)
WO (1) WO2018012963A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
LU100795B1 (en) * 2018-05-14 2019-11-14 Diatron MI PLC Immunoassay for whole blood samples
WO2020055246A1 (en) 2018-09-11 2020-03-19 Rijksuniversiteit Groningen Biological nanopores having tunable pore diameters and uses thereof as analytical tools
NL2023062B1 (en) 2019-05-03 2020-11-30 Univ Delft Tech Protein current trace signal acquisition using a nanopore
EP3771687A1 (en) 2019-07-30 2021-02-03 Rijksuniversiteit Groningen Reversible photo-controlled frac nanopores, modified frac monomers, methods for preparing the same, and uses thereof
US20220412948A1 (en) 2019-11-19 2022-12-29 Rijksuniversiteit Groningen Artificial nanopores and uses and methods relating thereto
US20230176034A1 (en) 2020-03-11 2023-06-08 Rijksuniversiteit Groningen Engineered PlyAB Nanopores and Uses Thereof
CN112480204A (zh) * 2020-04-13 2021-03-12 南京大学 一种采用Aerolysin纳米孔道的蛋白质/多肽测序方法
CN111413383B (zh) * 2020-04-20 2021-03-02 深圳市梅丽纳米孔科技有限公司 一种构建双识别位点纳米孔的方法
WO2022038144A1 (en) * 2020-08-18 2022-02-24 Kemijski inštitut Novel nanopore-forming polypeptides
US20240377380A1 (en) * 2021-03-31 2024-11-14 Illumina, Inc. Nanopore sensor devices
EP4326257A4 (en) * 2021-04-19 2025-03-12 ChemoCentryx, Inc. AZETIDINYLACETAMIDES AS CXCR7 INHIBITORS
WO2024078621A1 (zh) * 2022-10-14 2024-04-18 北京普译生物科技有限公司 一种pht纳米孔突变体蛋白及其应用
KR20250116014A (ko) 2022-10-28 2025-07-31 리엑스유니버시테이트 그로닝겐 나노기공-기반 단백질 분석
EP4662489A1 (en) * 2023-02-07 2025-12-17 Oxford University Innovation Limited Method of characterising a peptide, polypeptide or protein using a nanopore
CN116223583B (zh) * 2023-02-28 2026-04-28 中国科学院重庆绿色智能技术研究院 基于固态纳米孔技术的抗衰寡肽的单分子检测方法
GB202305524D0 (en) * 2023-04-14 2023-05-31 Oxford Nanopore Tech Plc Novel pore monomers and pores

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6362002B1 (en) * 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
CA2774710C (en) 2008-09-22 2016-08-02 University Of Washington Msp nanopores and related methods
WO2012009578A2 (en) 2010-07-14 2012-01-19 The Curators Of The University Of Missouri Nanopore-facilitated single molecule detection of nucleic acids

Also Published As

Publication number Publication date
CN109890980A (zh) 2019-06-14
JP2026021428A (ja) 2026-02-10
US20220242922A1 (en) 2022-08-04
US11312755B2 (en) 2022-04-26
JP2024105542A (ja) 2024-08-06
AU2017295442B2 (en) 2023-10-26
EP3485029A1 (en) 2019-05-22
ES2843099T3 (es) 2021-07-15
EP3828280A1 (en) 2021-06-02
WO2018012963A1 (en) 2018-01-18
US20190292235A1 (en) 2019-09-26
EP3485029B1 (en) 2020-11-11
AU2024200450A1 (en) 2024-02-15
CN119753114A (zh) 2025-04-04
AU2017295442A1 (en) 2019-02-07
AU2017295442A2 (en) 2019-02-21
JP7019665B2 (ja) 2022-02-15
AU2024200435A1 (en) 2024-02-08
JP2019533637A (ja) 2019-11-21
BR112019000680A2 (pt) 2019-04-24
JP2022064983A (ja) 2022-04-26

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