ES2843099T3 - Nanoporos biológicos para la detección y la secuenciación de biopolímeros basados en la actinoporina FraC - Google Patents
Nanoporos biológicos para la detección y la secuenciación de biopolímeros basados en la actinoporina FraC Download PDFInfo
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- ES2843099T3 ES2843099T3 ES17734851T ES17734851T ES2843099T3 ES 2843099 T3 ES2843099 T3 ES 2843099T3 ES 17734851 T ES17734851 T ES 17734851T ES 17734851 T ES17734851 T ES 17734851T ES 2843099 T3 ES2843099 T3 ES 2843099T3
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Classifications
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- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
- C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/416—Systems
- G01N27/447—Systems using electrophoresis
- G01N27/44756—Apparatus specially adapted therefor
- G01N27/44791—Microapparatus
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B82—NANOTECHNOLOGY
- B82Y—SPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
- B82Y5/00—Nanobiotechnology or nanomedicine, e.g. protein engineering or drug delivery
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
- C07K2319/22—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a Strep-tag
Landscapes
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- Chemical & Material Sciences (AREA)
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- Spectroscopy & Molecular Physics (AREA)
- Dispersion Chemistry (AREA)
- Electrochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662360999P | 2016-07-12 | 2016-07-12 | |
| PCT/NL2017/050331 WO2018012963A1 (en) | 2016-07-12 | 2017-05-24 | Biological nanopores for biopolymer sensing and sequencing based on frac actinoporin |
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| ES2843099T3 true ES2843099T3 (es) | 2021-07-15 |
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| EP (2) | EP3828280A1 (https=) |
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| CA (1) | CA3030704A1 (https=) |
| ES (1) | ES2843099T3 (https=) |
| WO (1) | WO2018012963A1 (https=) |
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| LU100795B1 (en) * | 2018-05-14 | 2019-11-14 | Diatron MI PLC | Immunoassay for whole blood samples |
| WO2020055246A1 (en) | 2018-09-11 | 2020-03-19 | Rijksuniversiteit Groningen | Biological nanopores having tunable pore diameters and uses thereof as analytical tools |
| NL2023062B1 (en) | 2019-05-03 | 2020-11-30 | Univ Delft Tech | Protein current trace signal acquisition using a nanopore |
| EP3771687A1 (en) | 2019-07-30 | 2021-02-03 | Rijksuniversiteit Groningen | Reversible photo-controlled frac nanopores, modified frac monomers, methods for preparing the same, and uses thereof |
| US20220412948A1 (en) | 2019-11-19 | 2022-12-29 | Rijksuniversiteit Groningen | Artificial nanopores and uses and methods relating thereto |
| US20230176034A1 (en) | 2020-03-11 | 2023-06-08 | Rijksuniversiteit Groningen | Engineered PlyAB Nanopores and Uses Thereof |
| CN112480204A (zh) * | 2020-04-13 | 2021-03-12 | 南京大学 | 一种采用Aerolysin纳米孔道的蛋白质/多肽测序方法 |
| CN111413383B (zh) * | 2020-04-20 | 2021-03-02 | 深圳市梅丽纳米孔科技有限公司 | 一种构建双识别位点纳米孔的方法 |
| WO2022038144A1 (en) * | 2020-08-18 | 2022-02-24 | Kemijski inštitut | Novel nanopore-forming polypeptides |
| US20240377380A1 (en) * | 2021-03-31 | 2024-11-14 | Illumina, Inc. | Nanopore sensor devices |
| EP4326257A4 (en) * | 2021-04-19 | 2025-03-12 | ChemoCentryx, Inc. | AZETIDINYLACETAMIDES AS CXCR7 INHIBITORS |
| WO2024078621A1 (zh) * | 2022-10-14 | 2024-04-18 | 北京普译生物科技有限公司 | 一种pht纳米孔突变体蛋白及其应用 |
| KR20250116014A (ko) | 2022-10-28 | 2025-07-31 | 리엑스유니버시테이트 그로닝겐 | 나노기공-기반 단백질 분석 |
| EP4662489A1 (en) * | 2023-02-07 | 2025-12-17 | Oxford University Innovation Limited | Method of characterising a peptide, polypeptide or protein using a nanopore |
| CN116223583B (zh) * | 2023-02-28 | 2026-04-28 | 中国科学院重庆绿色智能技术研究院 | 基于固态纳米孔技术的抗衰寡肽的单分子检测方法 |
| GB202305524D0 (en) * | 2023-04-14 | 2023-05-31 | Oxford Nanopore Tech Plc | Novel pore monomers and pores |
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| CA2774710C (en) | 2008-09-22 | 2016-08-02 | University Of Washington | Msp nanopores and related methods |
| WO2012009578A2 (en) | 2010-07-14 | 2012-01-19 | The Curators Of The University Of Missouri | Nanopore-facilitated single molecule detection of nucleic acids |
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| AU2017295442A2 (en) | 2019-02-21 |
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| AU2024200435A1 (en) | 2024-02-08 |
| JP2019533637A (ja) | 2019-11-21 |
| BR112019000680A2 (pt) | 2019-04-24 |
| CA3030704A1 (en) | 2018-01-18 |
| JP2022064983A (ja) | 2022-04-26 |
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