CA3028227A1 - Improved heterotrophic production methods for microbial biomass and bioproducts - Google Patents

Improved heterotrophic production methods for microbial biomass and bioproducts Download PDF

Info

Publication number
CA3028227A1
CA3028227A1 CA3028227A CA3028227A CA3028227A1 CA 3028227 A1 CA3028227 A1 CA 3028227A1 CA 3028227 A CA3028227 A CA 3028227A CA 3028227 A CA3028227 A CA 3028227A CA 3028227 A1 CA3028227 A1 CA 3028227A1
Authority
CA
Canada
Prior art keywords
cell
microalgal
heterotrophic
culture
biomass
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3028227A
Other languages
English (en)
French (fr)
Inventor
Robert J. Schurr
Adelheid R. Kuehnle
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
KUEHNLE AGROSYSTEMS Inc
Original Assignee
KUEHNLE AGROSYSTEMS Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by KUEHNLE AGROSYSTEMS Inc filed Critical KUEHNLE AGROSYSTEMS Inc
Publication of CA3028227A1 publication Critical patent/CA3028227A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P23/00—Preparation of compounds containing a cyclohexene ring having an unsaturated side chain containing at least ten carbon atoms bound by conjugated double bonds, e.g. carotenes
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/03—Algae
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/12—Unicellular algae; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P3/00—Preparation of elements or inorganic compounds except carbon dioxide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/026—Unsaturated compounds, i.e. alkenes, alkynes or allenes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/24—Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26—Ketones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P9/00—Preparation of organic compounds containing a metal or atom other than H, N, C, O, S or halogen
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5097—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving plant cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/405—Assays involving biological materials from specific organisms or of a specific nature from algae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Polymers & Plastics (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Botany (AREA)
  • Animal Husbandry (AREA)
  • Birds (AREA)
  • Pathology (AREA)
  • Biophysics (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Environmental Sciences (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Dentistry (AREA)
  • Insects & Arthropods (AREA)
  • Mycology (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
CA3028227A 2016-06-30 2017-06-30 Improved heterotrophic production methods for microbial biomass and bioproducts Pending CA3028227A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662356896P 2016-06-30 2016-06-30
US62/356,896 2016-06-30
PCT/US2017/040486 WO2018006068A1 (en) 2016-06-30 2017-06-30 Improved heterotrophic production methods for microbial biomass and bioproducts

Publications (1)

Publication Number Publication Date
CA3028227A1 true CA3028227A1 (en) 2018-01-04

Family

ID=59351112

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3028227A Pending CA3028227A1 (en) 2016-06-30 2017-06-30 Improved heterotrophic production methods for microbial biomass and bioproducts

Country Status (5)

Country Link
US (2) US11034968B2 (https=)
EP (1) EP3478850A1 (https=)
JP (3) JP7549444B2 (https=)
CA (1) CA3028227A1 (https=)
WO (1) WO2018006068A1 (https=)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11419350B2 (en) 2016-07-01 2022-08-23 Corbion Biotech, Inc. Feed ingredients comprising lysed microbial cells
US11377675B2 (en) * 2019-01-16 2022-07-05 Kuehnle Agrosystems, Inc. Subterranean microalgae for production of microbial biomass, substances, and compositions
WO2021057709A1 (zh) * 2019-09-23 2021-04-01 山东拜昂生物技术有限公司 一种培养雨生红球藻生产虾青素的方法
US20220356504A1 (en) * 2019-09-23 2022-11-10 Bioalgo (Wf) Co., Ltd. Method for producing astaxanthin by heterotrophic culture of haematococcus pluvialis
CN110804639B (zh) * 2019-11-27 2022-04-08 中国科学院天津工业生物技术研究所 促进微藻虾青素酯化的方法
KR102445369B1 (ko) * 2019-12-03 2022-09-21 한양대학교 산학협력단 미세조류로부터 기능성 오일을 추출하는 방법 및 기능성 오일을 포함하는 유화액
EP4210663B1 (en) * 2020-09-10 2026-04-29 The Procter & Gamble Company Late-stage product differentiation process for personal care products
USPP34488P3 (en) 2020-10-30 2022-08-09 Kuehnle Agrosystems, Inc. Haematococcus microalga named ‘KAS1601-WG7’
KR20240014474A (ko) * 2021-05-27 2024-02-01 알.피.쉐러 테크놀러지즈 엘엘씨 포르밀글리신 함유 폴리펩티드의 절단을 조절하는 방법
EP4122330A1 (en) * 2021-07-22 2023-01-25 Alver World SA Methods of growing microalgae & products thereof
WO2023074226A1 (ja) * 2021-10-27 2023-05-04 本田技研工業株式会社 緑藻クラミドモナスの培養方法
CN115024428B (zh) * 2022-06-15 2024-05-24 广州希望饲料有限公司 一种具有改善水禽免疫力与肠道的无抗饲料添加剂及其制备方法
US20260047991A1 (en) * 2022-08-08 2026-02-19 University Of Florida Research Foundation, Inc. Stimuli-responsive chromogenic materials, methods of making and methods of use
WO2024258936A2 (en) * 2023-06-13 2024-12-19 Kuehnle Agrosystems, Inc. Improved edible compositions comprising an improved chlamydomonas reinhardtii component
KR102897913B1 (ko) * 2023-12-26 2025-12-12 전제은 미세조류로부터 관심 생성물을 고수율로 수득하기 위한 방법
CN120183511A (zh) * 2025-05-22 2025-06-20 成都英德生物医药设备有限公司 生物反应器的动态优化方法以及系统

Family Cites Families (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US6680426B2 (en) 1991-01-07 2004-01-20 Auburn University Genetic engineering of plant chloroplasts
US5882849A (en) 1989-10-10 1999-03-16 Aquasearch, Inc. Method of control of Haematococcus spp, growth process
IL116995A (en) 1996-02-01 2000-08-31 Univ Ben Gurion Procedure for large-scale production of astaxanthin from haematococcus
WO2002010398A2 (en) 2000-07-31 2002-02-07 Hahn Frederick M Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
WO2003027267A1 (en) 2001-09-26 2003-04-03 Parry Nutraceuticals Ltd. Process to produce astaxanthin from haematococcus biomass
US7329789B1 (en) 2001-10-25 2008-02-12 Phasex Corporation Method for extraction and concentration of carotenoids using supercritical fluids
EP1361280A1 (en) 2002-05-08 2003-11-12 Wageningen University Process for continuous production and extraction of carotenoids from natural sources
EP1724357A4 (en) 2004-03-04 2010-11-17 Suntory Holdings Ltd PROCESS FOR THE MANUFACTURE OF LIPIDS CONTAINING ASTAXANTHIN
WO2006107736A1 (en) 2005-04-01 2006-10-12 Algaen Corporation Extractability and bioavailability of the natural antioxidant astaxanthin from a green alga, haematococcus pluvialis
EP2147113A1 (en) 2007-05-23 2010-01-27 Cognis IP Management GmbH Efficient astaxanthin production strains derived from haematococcus pluvialis
EP1995325A1 (en) 2007-05-23 2008-11-26 Cognis IP Management GmbH Efficient astaxanthin production strains derived from Haematococcus pluvialis
AU2009231810A1 (en) 2008-03-31 2009-10-08 Kuehnle Agrosystems, Inc. Nuclear based expression of genes for production of biofuels and process co-products in algae
US8278090B1 (en) 2008-07-03 2012-10-02 Solazyme, Inc. Heterotrophic cultivation of hydrocarbon-producing microalgae
CH701253A1 (de) 2009-06-12 2010-12-15 Mibelle Ag Verwendung eines Extraktes aus Schneealgen in kosmetischen oder dermatologischen Formulierungen.
ES2732493T3 (es) 2009-09-18 2019-11-22 Phycoil Biotechnology Int Inc Fermentación de microalgas usando iluminación controlada
US9428779B2 (en) * 2010-02-03 2016-08-30 Sapphire Energy, Inc. Transformation of algae for increasing lipid production
USPP23858P3 (en) 2011-04-18 2013-08-27 Karl Y. Biel Chlamydomonas reinhardtii alga variety named ‘DG8-108’
CN103571906B (zh) 2012-07-27 2018-12-11 上海泽元海洋生物技术有限公司 一种利用微藻高效生产虾青素的新方法
WO2014074772A1 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Mixotrophic, phototrophic, and heterotrophic combination methods and systems
RU2541455C1 (ru) * 2014-10-03 2015-02-10 Институт биологии южных морей им. А.О. Ковалевского Способ культивирования одноклеточной зеленой водоросли haematococcus pluvialis для получения астаксантина

Also Published As

Publication number Publication date
EP3478850A1 (en) 2019-05-08
JP2019527052A (ja) 2019-09-26
JP2022177001A (ja) 2022-11-30
WO2018006068A1 (en) 2018-01-04
US20210198680A1 (en) 2021-07-01
US11034968B2 (en) 2021-06-15
JP2025041773A (ja) 2025-03-26
US20180002711A1 (en) 2018-01-04
JP7549444B2 (ja) 2024-09-11

Similar Documents

Publication Publication Date Title
US20210198680A1 (en) Heterotrophic Production Methods for Microbial Biomass and Bioproducts
Ambati et al. Industrial potential of carotenoid pigments from microalgae: Current trends and future prospects
Jin et al. Microalgal biotechnology: carotenoid production by the green algae Dunaliella salina
Bhosale et al. Microbial xanthophylls
Shah et al. Astaxanthin-producing green microalga Haematococcus pluvialis: from single cell to high value commercial products
Cysewski et al. Industrial Production of Microalgal Cell‐Mass and Secondary Products‐Species of High Potential: Haematococcus
Johnson et al. Astaxanthin from microbial sources
Yusoff et al. Use of microalgae pigments in aquaculture
Schweiggert et al. Carotenoid production by bacteria, microalgae, and fungi
JP2001512030A (ja) 新規カロテノイド産生細菌種とそれを用いたカロテノイドの生産方法
Pérez‐Legaspi et al. Microalgal pigment induction and transfer in aquaculture
KR101847551B1 (ko) 카로티노이드 계열의 항산화 색소 및 dha를 포함한 바이오오일을 고생산하는 돌연변이 미세조류인 스키조키트리움 속 shg104 균주 및 이의 용도
Mostafa et al. Microalgae as a source of carotenoids in foods, obstacles and solutions
de Boer Biotechnological production of colorants
JP2003523765A (ja) 真核微生物を利用したカロテノイド、キサントフィルおよびアポ−カロテノイドの生産法
Niizawa et al. Astaxanthin production by autotrophic cultivation of Haematococcus pluvialis: A success story
US20130309719A1 (en) Heterotrophic microbial production of xanthophyll pigments
Dufossé Microbial and microalgal carotenoids as colourants and supplements
Tangerås et al. Coloring of salmonids in aquaculture: the yeast Phaffia rhodozyma as a source of astaxanthin
Zantioti et al. Use of pigmented fungi as additives in aquaculture
Dufossé Current carotenoid production using microorganisms
Kaur et al. Microalgae: a source of natural colours
Martins et al. Astaxanthin in microalgae Eustigmatophyceae
O’Callaghan Biotechnology in natural food colours: The role of bioprocessing
KR102897913B1 (ko) 미세조류로부터 관심 생성물을 고수율로 수득하기 위한 방법

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20220624

D15 Examination report completed

Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT

Effective date: 20241008

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION

Effective date: 20250206

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT

Effective date: 20250221

MFA Maintenance fee for application paid

Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 8TH ANNIV.) - STANDARD

Year of fee payment: 8

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20250618

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20250618

P11 Amendment of application requested

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT

Effective date: 20250703

P13 Application amended

Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED

Effective date: 20250703