CA3024355A1 - Methods for identification of samples - Google Patents
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- CA3024355A1 CA3024355A1 CA3024355A CA3024355A CA3024355A1 CA 3024355 A1 CA3024355 A1 CA 3024355A1 CA 3024355 A CA3024355 A CA 3024355A CA 3024355 A CA3024355 A CA 3024355A CA 3024355 A1 CA3024355 A1 CA 3024355A1
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Classifications
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C12Q2525/10—Modifications characterised by
- C12Q2525/179—Modifications characterised by incorporating arbitrary or random nucleotide sequences
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- C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2545/00—Reactions characterised by their quantitative nature
- C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
- C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
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| US11702653B2 (en) | 2018-05-21 | 2023-07-18 | Battelle Memorial Institute | Control compositions and methods for sequencing |
| WO2020124003A1 (en) * | 2018-12-13 | 2020-06-18 | Battelle Memorial Institute | Methods and control compositions for a quantitative polymerase chain reaction |
| US20220059187A1 (en) * | 2020-08-07 | 2022-02-24 | Oxford Nanopore Technologies Limited | Methods of detecting nucleic acid barcodes |
| AU2021356733B2 (en) * | 2020-10-06 | 2022-06-09 | Nucleotrace Pty. Ltd. | Oligonucleotides representing digital data |
| CN115812103A (zh) * | 2020-11-06 | 2023-03-17 | 伊鲁米纳公司 | 使用寡核苷酸检测混合物中的材料 |
| KR20220122095A (ko) * | 2021-02-26 | 2022-09-02 | 지니너스 주식회사 | 분자 바코딩 효율을 향상시키기 위한 조성물 및 이의 용도 |
| WO2022192603A1 (en) * | 2021-03-10 | 2022-09-15 | Factorial Diagnostics, Inc. | Cell barcoding compositions and methods |
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| US6030657A (en) | 1994-11-01 | 2000-02-29 | Dna Technologies, Inc. | Labeling technique for countering product diversion and product counterfeiting |
| US5776737A (en) | 1994-12-22 | 1998-07-07 | Visible Genetics Inc. | Method and composition for internal identification of samples |
| ATE347617T1 (de) | 1999-05-06 | 2006-12-15 | Sinai School Medicine | Steganographie auf dna basis |
| EP1515268A3 (en) * | 2001-03-01 | 2007-12-12 | NTT Data Technology Corporation | Method and system for individual authentication and digital signature utilizing article having DNA based ID information mark |
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| US20100285985A1 (en) | 2003-04-15 | 2010-11-11 | Applied Dna Sciences, Inc. | Methods and Systems for the Generation of Plurality of Security Markers and the Detection Therof |
| US8927213B2 (en) | 2004-12-23 | 2015-01-06 | Greg Hampikian | Reference markers for biological samples |
| WO2005093641A1 (en) * | 2004-03-26 | 2005-10-06 | Universite Libre De Bruxelles | Biological samples localisation, identification and tracking, system and method using electronic tag |
| US20150141264A1 (en) * | 2005-05-20 | 2015-05-21 | Apdn (B.V.I.) Inc. | In-field dna extraction, detection and authentication methods and systems therefor |
| US8785130B2 (en) | 2005-07-07 | 2014-07-22 | Bio-Id Diagnostic Inc. | Use of markers including nucleotide sequence based codes to monitor methods of detection and identification of genetic material |
| EP1924704B1 (en) | 2005-08-02 | 2011-05-25 | Rubicon Genomics, Inc. | Compositions and methods for processing and amplification of dna, including using multiple enzymes in a single reaction |
| WO2007133163A1 (en) * | 2006-05-11 | 2007-11-22 | Singular Id Pte Ltd | Method of identifying an object, an identification tag, an object adapted to be identified, and related device and system |
| US20080101666A1 (en) * | 2006-10-30 | 2008-05-01 | Cytyc Corporation | Automated imaging system with slide marking |
| CA2697640C (en) | 2007-09-21 | 2016-06-21 | Katholieke Universiteit Leuven | Tools and methods for genetic tests using next generation sequencing |
| AU2007254626A1 (en) * | 2007-12-21 | 2009-07-09 | Canon Kabushiki Kaisha | Data encoding and decoding using circular configurations of marks |
| GB2472371B (en) | 2009-04-24 | 2011-10-26 | Selectamark Security Systems Plc | Synthetic nucleotide containing compositions for use in security marking of property and/or for marking a thief or attacker |
| WO2012019765A1 (en) * | 2010-08-10 | 2012-02-16 | European Molecular Biology Laboratory (Embl) | Methods and systems for tracking samples and sample combinations |
| GB201100152D0 (en) * | 2011-01-06 | 2011-02-23 | Epistem Ltd | Genedrive RFID |
| EP2670863B1 (en) * | 2011-01-31 | 2018-06-27 | H. Hoffnabb-La Roche Ag | Methods of identifying multiple epitopes in cells |
| WO2012168803A2 (en) * | 2011-06-07 | 2012-12-13 | Koninklijke Philips Electronics N.V. | Providing nucleotide sequence data |
| CA2872017A1 (en) * | 2012-05-09 | 2013-11-14 | Apdn (B.V.I.) Inc. | Verification of physical encryption taggants using digital representatives and authentications thereof |
| JP6445426B2 (ja) * | 2012-05-10 | 2018-12-26 | ザ ジェネラル ホスピタル コーポレイション | ヌクレオチド配列を決定する方法 |
| WO2013177220A1 (en) * | 2012-05-21 | 2013-11-28 | The Scripps Research Institute | Methods of sample preparation |
| WO2014005184A1 (en) | 2012-07-06 | 2014-01-09 | Geneworks Technologies Pty Ltd | Method of identification using nucleic acid tags |
| EP2938742B1 (en) | 2012-12-28 | 2017-02-15 | Fleury S.A. | Method for complete tracking of a set of biological samples containing dna or rna through molecular barcode identification during laboratorial workflow and kit for collecting biological samples containing dna or rna |
| WO2014152775A2 (en) | 2013-03-14 | 2014-09-25 | Certirx Corporation | Nucleic acid-based authentication and identification codes |
| US20150141257A1 (en) | 2013-08-02 | 2015-05-21 | Roche Nimblegen, Inc. | Sequence capture method using specialized capture probes (heatseq) |
| CN105200530A (zh) * | 2015-10-13 | 2015-12-30 | 北京百迈客生物科技有限公司 | 一种适用于高通量全基因组测序的多样品混合文库的构建方法 |
| CN108449933A (zh) * | 2015-11-18 | 2018-08-24 | 宝生物工程（美国)有限公司 | 用于汇集来自多孔装置的样品的系统和方法 |
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| AU2023229558B2 (en) | 2025-07-31 |
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| EP3458606A1 (en) | 2019-03-27 |
| AU2023229558A1 (en) | 2023-09-28 |
| JP2019523652A (ja) | 2019-08-29 |
| EP3458606B1 (en) | 2025-04-02 |
| US12297495B2 (en) | 2025-05-13 |
| JP7071341B2 (ja) | 2022-05-18 |
| WO2017198742A1 (en) | 2017-11-23 |
| US20190300948A1 (en) | 2019-10-03 |
| KR102806552B1 (ko) | 2025-05-13 |
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