CA3016555A1 - Multiplexed immunohistochemistry using recombinant antibodies with epitope tags - Google Patents

Multiplexed immunohistochemistry using recombinant antibodies with epitope tags Download PDF

Info

Publication number
CA3016555A1
CA3016555A1 CA3016555A CA3016555A CA3016555A1 CA 3016555 A1 CA3016555 A1 CA 3016555A1 CA 3016555 A CA3016555 A CA 3016555A CA 3016555 A CA3016555 A CA 3016555A CA 3016555 A1 CA3016555 A1 CA 3016555A1
Authority
CA
Canada
Prior art keywords
epitope
seq
antibody
tag
tagged
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3016555A
Other languages
English (en)
French (fr)
Inventor
Michael Farrell
Eric May
Lei Tang
Wenjun Zhang
Antony HUBBARD
Tsu-Shuen Tsao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ventana Medical Systems Inc
Original Assignee
Ventana Medical Systems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ventana Medical Systems Inc filed Critical Ventana Medical Systems Inc
Publication of CA3016555A1 publication Critical patent/CA3016555A1/en
Pending legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6878Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids in epitope analysis
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2815Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against CD8
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2827Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against B7 molecules, e.g. CD80, CD86
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2887Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against CD20
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/32Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/40Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/40Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation
    • C07K2319/42Fusion polypeptide containing a tag for immunodetection, or an epitope for immunisation containing a HA(hemagglutinin)-tag
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2570/00Omics, e.g. proteomics, glycomics or lipidomics; Methods of analysis focusing on the entire complement of classes of biological molecules or subsets thereof, i.e. focusing on proteomes, glycomes or lipidomes

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Enzymes And Modification Thereof (AREA)
CA3016555A 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags Pending CA3016555A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US201662305440P 2016-03-08 2016-03-08
US62/305,440 2016-03-08
US201662418667P 2016-11-07 2016-11-07
US62/418,667 2016-11-07
US201762461651P 2017-02-21 2017-02-21
US62/461,651 2017-02-21
PCT/US2017/021157 WO2017155996A1 (en) 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Publications (1)

Publication Number Publication Date
CA3016555A1 true CA3016555A1 (en) 2017-09-14

Family

ID=58361148

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3016555A Pending CA3016555A1 (en) 2016-03-08 2017-03-07 Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Country Status (6)

Country Link
US (3) US11112413B2 (cg-RX-API-DMAC7.html)
EP (1) EP3426684B1 (cg-RX-API-DMAC7.html)
JP (3) JP6952706B2 (cg-RX-API-DMAC7.html)
AU (1) AU2017229370B2 (cg-RX-API-DMAC7.html)
CA (1) CA3016555A1 (cg-RX-API-DMAC7.html)
WO (1) WO2017155996A1 (cg-RX-API-DMAC7.html)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MA43135A (fr) 2015-10-30 2018-09-05 Aleta Biotherapeutics Inc Compositions et méthodes pour le du traitement du cancer
BR112018008390A2 (pt) 2015-10-30 2018-11-27 Aleta Biotherapeutics Inc composições e métodos para transdução tumoral
AU2017229370B2 (en) * 2016-03-08 2021-03-11 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
US20210188953A1 (en) * 2017-11-07 2021-06-24 Trustees Of Tufts College Heteromultimeric binding molecules for toxin neutralization, compositions and methods thereof
WO2020206175A1 (en) * 2019-04-03 2020-10-08 Access Medical Systems, Ltd. Method for re-using hapten-coated probe in an immunoassay
CN110850094A (zh) * 2019-11-22 2020-02-28 西安交通大学 一种免疫组化双标单染试剂盒及其使用方法和应用
CN114113616B (zh) * 2021-12-07 2022-08-23 深圳裕策生物科技有限公司 一种试剂盒及其染色方法
JP7783531B2 (ja) 2022-02-22 2025-12-10 Ntt株式会社 センサー付きハイドロゲル流路型デバイス
EP4590709A1 (en) 2022-09-22 2025-07-30 Agilent Technologies, Inc. Anti-human icos antibodies for use in immunohistochemistry (ihc) protocols and for diagnosing cancer

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1564604A (en) 1925-06-12 1925-12-08 Chas S Hodges Water wheel
US5595707A (en) 1990-03-02 1997-01-21 Ventana Medical Systems, Inc. Automated biological reaction apparatus
US20030211630A1 (en) 1998-02-27 2003-11-13 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
CA2320750C (en) 1998-02-27 2004-08-24 Ventana Medical Systems, Inc. System and method of aspirating and dispensing reagent
US6582962B1 (en) 1998-02-27 2003-06-24 Ventana Medical Systems, Inc. Automated molecular pathology apparatus having independent slide heaters
US6670113B2 (en) 2001-03-30 2003-12-30 Nanoprobes Enzymatic deposition and alteration of metals
JP4648902B2 (ja) 2003-06-24 2011-03-09 ベンタナ・メデイカル・システムズ・インコーポレーテツド 免疫組織化学的エピトープおよび核酸配列のインサイチュ検出が向上するように酵素の触媒作用で金属を付着
US20060246523A1 (en) 2005-04-28 2006-11-02 Christopher Bieniarz Antibody conjugates
WO2007062177A2 (en) 2005-11-23 2007-05-31 Ventana Medical Systems, Inc. Molecular conjugate
CA3069091C (en) 2006-11-01 2021-09-14 Ventana Medical Systems, Inc. Haptens, hapten conjugates, compositions thereof and method for their preparation and use
EP2167963B1 (en) 2007-05-23 2019-04-17 Ventana Medical Systems, Inc. Polymeric carriers for immunohistochemistry and in situ hybridization
US20080299555A1 (en) * 2007-05-30 2008-12-04 Hiroaki Nitta Multicolor chromogenic detection of biomarkers
WO2009111729A1 (en) * 2008-03-06 2009-09-11 Ventana Medical Systems, Inc. Anti-dnp antibodies and methods of use
AU2009282760B2 (en) 2008-08-22 2013-08-15 Ventana Medical Systems, Inc. Method for chromogenic detection of two or more target molecules in a single sample
AU2009311233B2 (en) * 2008-11-06 2015-01-15 University Of Guelph Methods of improving the therapeutic efficacy and utility of antibody fragments
KR101424414B1 (ko) 2009-10-19 2014-08-01 벤타나 메디컬 시스템즈, 인코포레이티드 현미경 스테이지에 대한 디바이스
WO2011139978A1 (en) 2010-05-04 2011-11-10 Ventana Medical Systems, Inc. Moving meniscus rinsing and mixing in cell staining
EP2588443A2 (en) 2010-07-02 2013-05-08 Ventana Medical Systems, Inc. Hapten conjugates for target detection
CN103097412B (zh) * 2010-07-09 2016-08-10 克鲁塞尔荷兰公司 抗人呼吸道合胞病毒(rsv)抗体以及使用方法
JP6644713B2 (ja) 2014-02-24 2020-02-12 ヴェンタナ メディカル システムズ, インク. キノンメチドアナログシグナル増幅
JP7095995B2 (ja) 2015-04-20 2022-07-05 ベンタナ メディカル システムズ, インコーポレイテッド 組織学的試料のための試薬のインクジェット沈着
AU2017229370B2 (en) * 2016-03-08 2021-03-11 Ventana Medical Systems, Inc. Multiplexed immunohistochemistry using recombinant antibodies with epitope tags

Also Published As

Publication number Publication date
JP2019512224A (ja) 2019-05-16
US11988673B2 (en) 2024-05-21
WO2017155996A1 (en) 2017-09-14
JP2023024990A (ja) 2023-02-21
JP6952706B2 (ja) 2021-10-20
JP7174126B2 (ja) 2022-11-17
AU2017229370B2 (en) 2021-03-11
EP3426684A1 (en) 2019-01-16
US11112413B2 (en) 2021-09-07
AU2017229370A1 (en) 2018-08-16
EP3426684B1 (en) 2024-10-09
US20190094242A1 (en) 2019-03-28
US20220057408A1 (en) 2022-02-24
JP2022003064A (ja) 2022-01-11
US20240230667A1 (en) 2024-07-11

Similar Documents

Publication Publication Date Title
US11988673B2 (en) Multiplexed immunohistochemistry using recombinant antibodies with epitope tags
US20220178934A1 (en) Signaling conjugates and methods of use
AU2016357478B2 (en) Methods of identifying immune cells in PD-L1 positive tumor tissue
CA2900842A1 (en) Digitally enhanced microscopy for multiplexed histology
US20230151361A1 (en) Peptide nucleic acid conjugates
US20250321232A1 (en) Chromogenic multiplexing methods and systems for identifying a cancer of unknown primary origin
JP2024526175A (ja) 目的の分子を検出するための脱パラフィン細胞から出発する単一細胞elisaの使用

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102

EEER Examination request

Effective date: 20200102