CA3007447C - Methods and kits for joining fragmented nucleic acids together - Google Patents

Methods and kits for joining fragmented nucleic acids together Download PDF

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Publication number
CA3007447C
CA3007447C CA3007447A CA3007447A CA3007447C CA 3007447 C CA3007447 C CA 3007447C CA 3007447 A CA3007447 A CA 3007447A CA 3007447 A CA3007447 A CA 3007447A CA 3007447 C CA3007447 C CA 3007447C
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Canada
Prior art keywords
nucleic acid
sense strand
target nucleic
primers
sample
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Active
Application number
CA3007447A
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English (en)
French (fr)
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CA3007447A1 (en
Inventor
Stephen G. Will
Yuker Wang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Publication of CA3007447A1 publication Critical patent/CA3007447A1/en
Application granted granted Critical
Publication of CA3007447C publication Critical patent/CA3007447C/en
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2533/00—Reactions characterised by the enzymatic reaction principle used
    • C12Q2533/10—Reactions characterised by the enzymatic reaction principle used the purpose being to increase the length of an oligonucleotide strand
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/101—Homogeneous assay format, e.g. one pot reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA3007447A 2015-12-10 2016-12-08 Methods and kits for joining fragmented nucleic acids together Active CA3007447C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14/965,343 2015-12-10
US14/965,343 US10119161B2 (en) 2015-12-10 2015-12-10 Methods and kits for joining fragmented nucleic acids together
PCT/EP2016/080225 WO2017097887A1 (en) 2015-12-10 2016-12-08 Methods and kits for joining fragmented nucleic acids together

Publications (2)

Publication Number Publication Date
CA3007447A1 CA3007447A1 (en) 2017-06-15
CA3007447C true CA3007447C (en) 2022-04-19

Family

ID=57517894

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3007447A Active CA3007447C (en) 2015-12-10 2016-12-08 Methods and kits for joining fragmented nucleic acids together

Country Status (7)

Country Link
US (1) US10119161B2 (https=)
EP (1) EP3387146B1 (https=)
JP (1) JP6986015B2 (https=)
CN (1) CN108291255B (https=)
CA (1) CA3007447C (https=)
ES (1) ES2886602T3 (https=)
WO (1) WO2017097887A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20250144226A1 (en) * 2018-04-11 2025-05-08 Design Therapeutics, Inc. Methods and compounds for the treatment of genetic disease
US20210283265A1 (en) * 2018-04-20 2021-09-16 Design Therapeutics, Inc. Methods and compounds for the treatment of genetic disease
JP7077992B2 (ja) * 2019-02-22 2022-05-31 横河電機株式会社 核酸配列計測用デバイス、核酸配列計測方法、および核酸配列計測装置

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999016904A1 (en) * 1997-09-29 1999-04-08 City Of Hope Efficient linking of nucleic acid segments
CN100569956C (zh) * 2006-09-11 2009-12-16 中山大学达安基因股份有限公司 检测结核分枝杆菌及其耐药基因突变的方法和试剂盒
CN102242211A (zh) * 2011-07-08 2011-11-16 无锡锐奇基因生物科技有限公司 检测突变核酸序列的方法及试剂盒
ITMI20112177A1 (it) * 2011-11-29 2013-05-30 Genefast S R L Metodo per rilevare la sintesi e/o l'amplificazione di un acido nucleico
CN103255227B (zh) * 2013-05-30 2015-02-25 上海快灵生物科技有限公司 引物介导环化的恒温核酸滚环扩增方法及试剂盒

Also Published As

Publication number Publication date
EP3387146B1 (en) 2021-06-30
JP2019502374A (ja) 2019-01-31
ES2886602T3 (es) 2021-12-20
JP6986015B2 (ja) 2021-12-22
US20170166953A1 (en) 2017-06-15
CN108291255B (zh) 2022-03-11
WO2017097887A1 (en) 2017-06-15
US10119161B2 (en) 2018-11-06
EP3387146A1 (en) 2018-10-17
CN108291255A (zh) 2018-07-17
CA3007447A1 (en) 2017-06-15

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