CA3007066A1 - Methods for gender determination of avian embryos in unhatched eggs and means thereof - Google Patents

Methods for gender determination of avian embryos in unhatched eggs and means thereof Download PDF

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Publication number
CA3007066A1
CA3007066A1 CA3007066A CA3007066A CA3007066A1 CA 3007066 A1 CA3007066 A1 CA 3007066A1 CA 3007066 A CA3007066 A CA 3007066A CA 3007066 A CA3007066 A CA 3007066A CA 3007066 A1 CA3007066 A1 CA 3007066A1
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Canada
Prior art keywords
acid sequence
reporter gene
nucleic acid
seq
denoted
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA3007066A
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English (en)
French (fr)
Inventor
Daniel Offen
Original Assignee
Eggxyt Ltd
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Filing date
Publication date
Application filed by Eggxyt Ltd filed Critical Eggxyt Ltd
Publication of CA3007066A1 publication Critical patent/CA3007066A1/en
Abandoned legal-status Critical Current

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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/66—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving luciferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6879—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for sex determination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/30—Bird
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/03—Animal model, e.g. for test or diseases
    • A01K2267/0393—Animal model comprising a reporter system for screening tests
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
CA3007066A 2015-12-03 2016-12-01 Methods for gender determination of avian embryos in unhatched eggs and means thereof Abandoned CA3007066A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562262409P 2015-12-03 2015-12-03
US62/262,409 2015-12-03
PCT/IL2016/051291 WO2017094015A1 (en) 2015-12-03 2016-12-01 Methods for gender determination of avian embryos in unhatched eggs and means thereof

Publications (1)

Publication Number Publication Date
CA3007066A1 true CA3007066A1 (en) 2017-06-08

Family

ID=58796444

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3007066A Abandoned CA3007066A1 (en) 2015-12-03 2016-12-01 Methods for gender determination of avian embryos in unhatched eggs and means thereof

Country Status (9)

Country Link
US (1) US20190029236A1 (cg-RX-API-DMAC10.html)
EP (1) EP3384051A4 (cg-RX-API-DMAC10.html)
JP (1) JP2019505175A (cg-RX-API-DMAC10.html)
KR (1) KR20180099704A (cg-RX-API-DMAC10.html)
CN (1) CN108474034A (cg-RX-API-DMAC10.html)
CA (1) CA3007066A1 (cg-RX-API-DMAC10.html)
HK (1) HK1257753A1 (cg-RX-API-DMAC10.html)
IL (2) IL259721B (cg-RX-API-DMAC10.html)
WO (1) WO2017094015A1 (cg-RX-API-DMAC10.html)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107760715A (zh) * 2017-10-17 2018-03-06 云南瑞火生物科技有限公司 一种精准高效的转基因载体及其构建方法和应用
CN111315212A (zh) * 2017-09-19 2020-06-19 以色列农业和农村发展部农业研究组织(范卡尼中心) 经过基因组编辑的鸟

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201911686YA (en) * 2017-05-25 2020-01-30 Eggxyt Ltd Methods for gender determination of avian embryos in unhatched eggs and means thereof
JP7493940B2 (ja) * 2017-05-31 2024-06-03 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガナイゼーション 鳥類における形質選択
WO2019135230A1 (en) * 2018-01-04 2019-07-11 Ramot At Tel-Aviv University Ltd. Transgenic eukaryotic organisms and methods for gender selection
DE102018105512A1 (de) 2018-03-09 2019-09-12 Technische Universitaet Dresden Verfahren zur in-ovo fertilisations- und geschlechtsbestimmung am geschlossenen ei
EP3599465A1 (en) * 2018-07-24 2020-01-29 Tronico Method for determining a specific characteristic of an embryo in an unhatched egg
CN109402244B (zh) * 2018-12-20 2022-05-03 广西大学 一种哺乳动物胚胎性别鉴定方法
MX2021010611A (es) 2019-03-05 2021-11-12 The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo Aves con genoma editado.
CN111073963B (zh) * 2019-12-10 2023-04-14 南京农业大学 一种一日龄鉴别鸡受精蛋性别的方法
JP2023531285A (ja) * 2020-06-26 2023-07-21 サニーブルック リサーチ インスティテュート 培養培地のための卵黄抽出補助物質及び関連する方法
CN114793988B (zh) * 2022-04-19 2023-01-17 中国科学院海洋研究所 一种基于形态学鉴定孔鳐受精卵的方法
CN115960945B (zh) * 2022-12-05 2024-11-08 天津科技大学 蓝光诱导酿酒酵母定点dsb系统的构建

Family Cites Families (13)

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JPH06124456A (ja) 1992-10-09 1994-05-06 Sony Corp 光学ピックアップ装置
GB9511439D0 (en) 1995-06-06 1995-08-02 Isis Innovation Gene product and method
AUPO060996A0 (en) 1996-06-21 1996-07-18 University Of Melbourne, The Sexing gene
CA2264450A1 (en) * 1998-03-05 1999-09-05 Andras Nagy Methods for monitoring heterologous sex chromosomes
US6244214B1 (en) 1999-01-06 2001-06-12 Embrex, Inc. Concurrent in ovo injection and detection method and apparatus
GB0218955D0 (en) * 2002-08-14 2002-09-25 Roslin Inst Edinburgh Avian sex determination method
JP2012511915A (ja) * 2008-12-17 2012-05-31 コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼイション 鳥類の性別の調節方法
US20120084873A1 (en) * 2009-02-08 2012-04-05 Andrew Sinclair Sex-determination and methods of specifying same
CN102421282B (zh) * 2009-03-13 2015-06-10 范德冯管理有限公司 鸟类性别判定的方法
FR2969497B1 (fr) 2010-12-27 2013-06-28 Ceva Sante Animale Composition luminescente comme biomarqueur dans un oeuf aviaire, dispositif et procede correspondants.
PL2880440T3 (pl) * 2012-07-30 2019-05-31 In Ovo B V Ustalanie płci, żywotności i/lub stadium rozwojowego zarodków ptasich in ovo
WO2014033544A2 (en) 2012-08-31 2014-03-06 Mat Malta Advanced Technologies Limited Spectrophotometric analysis of embryonic chick feather color
FR3023617B1 (fr) 2014-07-11 2018-06-15 Tronico Dispositif non invasif de determination de la fertilite et/ou du sexe d’un oeuf, et procede correspondant.

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111315212A (zh) * 2017-09-19 2020-06-19 以色列农业和农村发展部农业研究组织(范卡尼中心) 经过基因组编辑的鸟
CN107760715A (zh) * 2017-10-17 2018-03-06 云南瑞火生物科技有限公司 一种精准高效的转基因载体及其构建方法和应用
CN107760715B (zh) * 2017-10-17 2021-12-10 张业胜 一种转基因载体及其构建方法和应用

Also Published As

Publication number Publication date
IL259721A (en) 2018-07-31
KR20180099704A (ko) 2018-09-05
IL259721B (en) 2021-12-01
CN108474034A (zh) 2018-08-31
EP3384051A1 (en) 2018-10-10
WO2017094015A1 (en) 2017-06-08
HK1257753A1 (zh) 2019-10-25
IL288409A (en) 2022-01-01
EP3384051A4 (en) 2019-04-24
US20190029236A1 (en) 2019-01-31
JP2019505175A (ja) 2019-02-28

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EEER Examination request

Effective date: 20211115

FZDE Discontinued

Effective date: 20240422