CA2991195C - Novel p450-bm3 variants with improved activity - Google Patents

Novel p450-bm3 variants with improved activity Download PDF

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Publication number
CA2991195C
CA2991195C CA2991195A CA2991195A CA2991195C CA 2991195 C CA2991195 C CA 2991195C CA 2991195 A CA2991195 A CA 2991195A CA 2991195 A CA2991195 A CA 2991195A CA 2991195 C CA2991195 C CA 2991195C
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sequence
ton
polypeptide
cony
amino acid
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CA2991195A
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English (en)
French (fr)
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CA2991195A1 (en
Inventor
Robert Osborne
Vesna Mitchell
Khin Yu Naing Htwe
Xiyun Zhang
Erika M. Milczek
Jeffrey C. Moore
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Codexis Inc
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Codexis Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0036—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6)
    • C12N9/0038—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12N9/0042—NADPH-cytochrome P450 reductase (1.6.2.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y106/00—Oxidoreductases acting on NADH or NADPH (1.6)
    • C12Y106/02—Oxidoreductases acting on NADH or NADPH (1.6) with a heme protein as acceptor (1.6.2)
    • C12Y106/02004—NADPH-hemoprotein reductase (1.6.2.4), i.e. NADP-cytochrome P450-reductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/14—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen (1.14.14)
    • C12Y114/14001—Unspecific monooxygenase (1.14.14.1)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2991195A 2015-07-07 2016-06-02 Novel p450-bm3 variants with improved activity Active CA2991195C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201562189281P 2015-07-07 2015-07-07
US62/189,281 2015-07-07
PCT/US2016/035399 WO2017007547A1 (en) 2015-07-07 2016-06-02 Novel p450-bm3 variants with improved activity

Publications (2)

Publication Number Publication Date
CA2991195A1 CA2991195A1 (en) 2017-01-12
CA2991195C true CA2991195C (en) 2021-06-29

Family

ID=57686072

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2991195A Active CA2991195C (en) 2015-07-07 2016-06-02 Novel p450-bm3 variants with improved activity

Country Status (8)

Country Link
US (7) US9683220B2 (cg-RX-API-DMAC7.html)
EP (1) EP3319987B1 (cg-RX-API-DMAC7.html)
JP (1) JP6691558B2 (cg-RX-API-DMAC7.html)
CN (1) CN108271374B (cg-RX-API-DMAC7.html)
CA (1) CA2991195C (cg-RX-API-DMAC7.html)
DK (1) DK3319987T3 (cg-RX-API-DMAC7.html)
IL (1) IL256441B (cg-RX-API-DMAC7.html)
WO (1) WO2017007547A1 (cg-RX-API-DMAC7.html)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9708587B2 (en) 2014-07-09 2017-07-18 Codexis, Inc. P450-BM3 variants with improved activity
JP6691558B2 (ja) 2015-07-07 2020-04-28 コデクシス， インコーポレイテッド 改善された活性を持つ新規のｐ４５０−ｂｍ３バリアント
SI3370733T1 (sl) * 2015-11-02 2021-11-30 Board Of Regents The University Of Texas System Postopek za aktivacijo CD40 in blokada imunske nadzorne točke
WO2019183358A2 (en) * 2018-03-21 2019-09-26 University Of Florida Research Foundation, Incorporated Whole cell processes to produce nitroaromatics
WO2024112781A1 (en) * 2022-11-22 2024-05-30 Codexis, Inc. P450-bm3 variants with improved activity
WO2024196434A1 (en) * 2023-03-20 2024-09-26 Codexis, Inc. P450-bm3 variants with improved activity

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Also Published As

Publication number Publication date
WO2017007547A1 (en) 2017-01-12
CA2991195A1 (en) 2017-01-12
JP6691558B2 (ja) 2020-04-28
DK3319987T3 (da) 2021-06-21
US9683220B2 (en) 2017-06-20
JP2018520674A (ja) 2018-08-02
EP3319987B1 (en) 2021-05-05
CN108271374A (zh) 2018-07-10
US20200002685A1 (en) 2020-01-02
EP3319987A1 (en) 2018-05-16
CN108271374B (zh) 2021-09-21
US10704030B2 (en) 2020-07-07
EP3319987A4 (en) 2018-12-19
US10450550B2 (en) 2019-10-22
US20210214694A1 (en) 2021-07-15
US20230250403A1 (en) 2023-08-10
US10982197B2 (en) 2021-04-20
IL256441B (en) 2020-02-27
IL256441A (en) 2018-02-28
US20170009213A1 (en) 2017-01-12
US20190024059A1 (en) 2019-01-24
US11591578B2 (en) 2023-02-28
US20170247667A1 (en) 2017-08-31
US10113153B2 (en) 2018-10-30
US20200291365A1 (en) 2020-09-17

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