CA2975190C - Epcam aptamers and conjugates thereof - Google Patents

Epcam aptamers and conjugates thereof Download PDF

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Publication number
CA2975190C
CA2975190C CA2975190A CA2975190A CA2975190C CA 2975190 C CA2975190 C CA 2975190C CA 2975190 A CA2975190 A CA 2975190A CA 2975190 A CA2975190 A CA 2975190A CA 2975190 C CA2975190 C CA 2975190C
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Canada
Prior art keywords
aptamer
epcam
cells
aptamer conjugate
brain
Prior art date
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Active
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CA2975190A
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English (en)
French (fr)
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CA2975190A1 (en
Inventor
Sarah SHIGDAR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deakin University
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Deakin University
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Filing date
Publication date
Priority claimed from AU2015900437A external-priority patent/AU2015900437A0/en
Application filed by Deakin University filed Critical Deakin University
Publication of CA2975190A1 publication Critical patent/CA2975190A1/en
Application granted granted Critical
Publication of CA2975190C publication Critical patent/CA2975190C/en
Active legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/51—Physical structure in polymeric form, e.g. multimers, concatemers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Epidemiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2975190A 2015-02-11 2016-02-11 Epcam aptamers and conjugates thereof Active CA2975190C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2015900437 2015-02-11
AU2015900437A AU2015900437A0 (en) 2015-02-11 Aptamer conjugate
PCT/AU2016/050085 WO2016127216A1 (en) 2015-02-11 2016-02-11 Epcam aptamers and conjugates thereof

Publications (2)

Publication Number Publication Date
CA2975190A1 CA2975190A1 (en) 2016-08-18
CA2975190C true CA2975190C (en) 2023-06-06

Family

ID=56613973

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2975190A Active CA2975190C (en) 2015-02-11 2016-02-11 Epcam aptamers and conjugates thereof

Country Status (7)

Country Link
US (1) US10577610B2 (https=)
EP (1) EP3256593B1 (https=)
JP (1) JP6978317B2 (https=)
AU (1) AU2016218945B2 (https=)
CA (1) CA2975190C (https=)
ES (1) ES2796504T3 (https=)
WO (1) WO2016127216A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2985036T3 (es) 2014-08-29 2024-11-04 Childrens Medical Ct Corp Métodos y composiciones para el tratamiento del cáncer
CN107249644A (zh) 2014-10-15 2017-10-13 希望之城 针对转铁蛋白受体(TfR)的RNA适体
EP3416690A4 (en) 2016-02-19 2020-02-19 City of Hope SPECIFIC APTAMER
US11332747B2 (en) * 2017-02-24 2022-05-17 Augusta University Research Institute, Inc. Bispecific aptamer for treating cancer
WO2019033051A1 (en) * 2017-08-11 2019-02-14 City Of Hope RNA APTAMERS AGAINST THE TRANSFERRIN RECEPTOR (TFR)
EP3827083A2 (en) * 2018-07-26 2021-06-02 aratinga. bio TNP Aptamer-based car t-cell switch
WO2020021338A2 (en) * 2018-07-26 2020-01-30 Aratinga.Bio Tnp Aptamer-based car t-cell switch
WO2020257401A1 (en) 2019-06-21 2020-12-24 The Children's Medical Center Corporation Methods and compositions for the treatment of cancer
KR102294694B1 (ko) * 2019-07-18 2021-08-30 국립암센터 신규한 dna 앱타머 및 이의 용도
CN110592093B (zh) * 2019-09-10 2023-08-25 上海交通大学医学院附属仁济医院 一种能够识别EpCAM蛋白的核酸适体及其制备方法与应用
CN112843261B (zh) * 2021-03-10 2022-11-01 中国人民解放军空军军医大学 靶向EpCAM的放射性配合物及其制备方法
JP7359457B2 (ja) * 2021-10-11 2023-10-11 タグシクス・バイオ株式会社 自己免疫性疾患治療薬

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070178451A1 (en) * 2001-08-02 2007-08-02 Molian Deng Nucleic acid sequences from Chlorella sarokiniana and uses thereof
KR20070101227A (ko) 2004-09-07 2007-10-16 아케믹스 코포레이션 폰 빌레브란트 인자에 대한 앱타머 및 이의 혈전증치료제로서의 용도
US20110052697A1 (en) 2006-05-17 2011-03-03 Gwangju Institute Of Science & Technology Aptamer-Directed Drug Delivery
EP2159286A1 (en) 2008-09-01 2010-03-03 Consiglio Nazionale Delle Ricerche Method for obtaining oligonucleotide aptamers and uses thereof
WO2013003898A1 (en) 2011-07-07 2013-01-10 Clinical Genomics Pty. Ltd Method for detection of cancer in a patient
WO2013163303A2 (en) * 2012-04-25 2013-10-31 Albert Einstein College Of Medicine Of Yeshiva University Transferrin receptor aptamers and aptamer-targeted delivery
EP2880185B1 (en) 2012-08-02 2019-07-03 Deakin University Cd133 aptamers for detection of cancer stem cells
JP6297034B2 (ja) * 2012-08-02 2018-03-20 ディーキン・ユニバーシティー 癌幹細胞を検出するためのＥｐＣＡＭアプタマー
CN103387989B (zh) * 2012-09-24 2016-05-18 厦门大学 上皮细胞粘附分子的核酸适体EpCAM D及其制备方法
AU2013340414B2 (en) * 2012-10-23 2019-05-02 Caris Science, Inc. Aptamers and uses thereof
US20160091489A1 (en) * 2013-06-03 2016-03-31 University Of Florida Research Foundation, Incorporated Devices and methods for isolating cells

Also Published As

Publication number Publication date
JP2018506284A (ja) 2018-03-08
JP6978317B2 (ja) 2021-12-08
EP3256593A1 (en) 2017-12-20
EP3256593B1 (en) 2020-03-25
EP3256593A4 (en) 2018-12-05
US10577610B2 (en) 2020-03-03
ES2796504T3 (es) 2020-11-27
CA2975190A1 (en) 2016-08-18
US20180037892A1 (en) 2018-02-08
AU2016218945A1 (en) 2017-08-03
WO2016127216A1 (en) 2016-08-18
AU2016218945B2 (en) 2021-08-26

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