CA2975190C - Epcam aptamers and conjugates thereof - Google Patents

Epcam aptamers and conjugates thereof Download PDF

Info

Publication number
CA2975190C
CA2975190C CA2975190A CA2975190A CA2975190C CA 2975190 C CA2975190 C CA 2975190C CA 2975190 A CA2975190 A CA 2975190A CA 2975190 A CA2975190 A CA 2975190A CA 2975190 C CA2975190 C CA 2975190C
Authority
CA
Canada
Prior art keywords
aptamer
epcam
cells
aptamer conjugate
brain
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2975190A
Other languages
English (en)
French (fr)
Other versions
CA2975190A1 (en
Inventor
Sarah SHIGDAR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Deakin University
Original Assignee
Deakin University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2015900437A external-priority patent/AU2015900437A0/en
Application filed by Deakin University filed Critical Deakin University
Publication of CA2975190A1 publication Critical patent/CA2975190A1/en
Application granted granted Critical
Publication of CA2975190C publication Critical patent/CA2975190C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/51—Physical structure in polymeric form, e.g. multimers, concatemers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Epidemiology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CA2975190A 2015-02-11 2016-02-11 Epcam aptamers and conjugates thereof Active CA2975190C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2015900437 2015-02-11
AU2015900437A AU2015900437A0 (en) 2015-02-11 Aptamer conjugate
PCT/AU2016/050085 WO2016127216A1 (en) 2015-02-11 2016-02-11 Epcam aptamers and conjugates thereof

Publications (2)

Publication Number Publication Date
CA2975190A1 CA2975190A1 (en) 2016-08-18
CA2975190C true CA2975190C (en) 2023-06-06

Family

ID=56613973

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2975190A Active CA2975190C (en) 2015-02-11 2016-02-11 Epcam aptamers and conjugates thereof

Country Status (7)

Country Link
US (1) US10577610B2 (https=)
EP (1) EP3256593B1 (https=)
JP (1) JP6978317B2 (https=)
AU (1) AU2016218945B2 (https=)
CA (1) CA2975190C (https=)
ES (1) ES2796504T3 (https=)
WO (1) WO2016127216A1 (https=)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107106704A (zh) * 2014-08-29 2017-08-29 儿童医疗中心有限公司 用于治疗癌症的方法和组合物
WO2016061386A1 (en) 2014-10-15 2016-04-21 City Of Hope Rna aptamers against transferrin receptor (tfr)
US11219635B2 (en) * 2016-02-19 2022-01-11 City Of Hope Bi-specific aptamer
US11332747B2 (en) * 2017-02-24 2022-05-17 Augusta University Research Institute, Inc. Bispecific aptamer for treating cancer
KR20200068647A (ko) * 2017-08-11 2020-06-15 시티 오브 호프 트랜스페린 수용체 (TfR)에 대한 RNA 앱타머
CN112955554A (zh) * 2018-07-26 2021-06-11 奥拉廷加Tnp生物 基于适体的car t细胞开关
KR102905569B1 (ko) 2018-07-26 2025-12-31 아라팅가 바이오 티엔피 압타머-기반 car t-세포 스위치
WO2020257401A1 (en) 2019-06-21 2020-12-24 The Children's Medical Center Corporation Methods and compositions for the treatment of cancer
KR102294694B1 (ko) * 2019-07-18 2021-08-30 국립암센터 신규한 dna 앱타머 및 이의 용도
CN110592093B (zh) * 2019-09-10 2023-08-25 上海交通大学医学院附属仁济医院 一种能够识别EpCAM蛋白的核酸适体及其制备方法与应用
CN112843261B (zh) * 2021-03-10 2022-11-01 中国人民解放军空军军医大学 靶向EpCAM的放射性配合物及其制备方法
JP7359457B2 (ja) * 2021-10-11 2023-10-11 タグシクス・バイオ株式会社 自己免疫性疾患治療薬

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070178451A1 (en) * 2001-08-02 2007-08-02 Molian Deng Nucleic acid sequences from Chlorella sarokiniana and uses thereof
EP1789096A4 (en) 2004-09-07 2009-07-08 Archemix Corp APTAMERS FOR THE VON WILLEBRAND FACTOR AND ITS USE AS THERAPEUTICS FOR THROMBOTIC DISEASES
WO2007137117A2 (en) 2006-05-17 2007-11-29 Massachusetts Institute Of Technology Aptamer-directed drug delivery
EP2159286A1 (en) 2008-09-01 2010-03-03 Consiglio Nazionale Delle Ricerche Method for obtaining oligonucleotide aptamers and uses thereof
WO2013003898A1 (en) 2011-07-07 2013-01-10 Clinical Genomics Pty. Ltd Method for detection of cancer in a patient
US9439973B2 (en) * 2012-04-25 2016-09-13 Albert Einstein College Of Medicine, Inc. Transferrin receptor aptamers and aptamer-targeted delivery
NZ704719A (en) 2012-08-02 2018-02-23 Univ Deakin Cd133 aptamers for detection of cancer stem cells
AU2013299332B2 (en) * 2012-08-02 2018-08-09 Deakin University EpCAM aptamer for detection of cancer stem cells
CN103387990B (zh) * 2012-09-24 2016-01-06 厦门大学 上皮细胞粘附分子的核酸适体EpCAM B及其制备方法
EP4170031A1 (en) 2012-10-23 2023-04-26 Caris Science, Inc. Aptamers and uses thereof
US20160091489A1 (en) * 2013-06-03 2016-03-31 University Of Florida Research Foundation, Incorporated Devices and methods for isolating cells

Also Published As

Publication number Publication date
JP6978317B2 (ja) 2021-12-08
ES2796504T3 (es) 2020-11-27
JP2018506284A (ja) 2018-03-08
EP3256593B1 (en) 2020-03-25
CA2975190A1 (en) 2016-08-18
AU2016218945A1 (en) 2017-08-03
AU2016218945B2 (en) 2021-08-26
US20180037892A1 (en) 2018-02-08
US10577610B2 (en) 2020-03-03
EP3256593A1 (en) 2017-12-20
WO2016127216A1 (en) 2016-08-18
EP3256593A4 (en) 2018-12-05

Similar Documents

Publication Publication Date Title
CA2975190C (en) Epcam aptamers and conjugates thereof
CA2880590C (en) Epcam aptamer for detection of cancer stem cells
EP2880185B1 (en) Cd133 aptamers for detection of cancer stem cells
EP3102708B1 (en) Aptamer construct
HK1231933B (en) Aptamer construct

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20201118

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 9TH ANNIV.) - STANDARD

Year of fee payment: 9

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20241203

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20241203

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20241203

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 10TH ANNIV.) - STANDARD

Year of fee payment: 10

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20260204

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20260205