CA2971268A1 - Detection of nucleic acid polymerase conformational changes using a nanotube - Google Patents
Detection of nucleic acid polymerase conformational changes using a nanotube Download PDFInfo
- Publication number
- CA2971268A1 CA2971268A1 CA2971268A CA2971268A CA2971268A1 CA 2971268 A1 CA2971268 A1 CA 2971268A1 CA 2971268 A CA2971268 A CA 2971268A CA 2971268 A CA2971268 A CA 2971268A CA 2971268 A1 CA2971268 A1 CA 2971268A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- thio
- nucleotide
- molecule
- nucleotide analog
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
- C12Y207/07—Nucleotidyltransferases (2.7.7)
- C12Y207/07007—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
- G01N27/403—Cells and electrode assemblies
- G01N27/414—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS
- G01N27/4146—Ion-sensitive or chemical field-effect transistors, i.e. ISFETS or CHEMFETS involving nanosized elements, e.g. nanotubes, nanowires
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
- G01N33/48721—Investigating individual macromolecules, e.g. by translocation through nanopores
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/90—Enzymes; Proenzymes
- G01N2333/91—Transferases (2.)
- G01N2333/912—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- G01N2333/91205—Phosphotransferases in general
- G01N2333/91245—Nucleotidyltransferases (2.7.7)
- G01N2333/9125—Nucleotidyltransferases (2.7.7) with a definite EC number (2.7.7.-)
- G01N2333/9126—DNA-directed DNA polymerase (2.7.7.7)
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Nanotechnology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microelectronics & Electronic Packaging (AREA)
- Electrochemistry (AREA)
- Biomedical Technology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201462093671P | 2014-12-18 | 2014-12-18 | |
| US62/093,671 | 2014-12-18 | ||
| PCT/US2015/066321 WO2016100635A1 (en) | 2014-12-18 | 2015-12-17 | Detection of nucleic acid polymerase conformational changes using a nanotube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2971268A1 true CA2971268A1 (en) | 2016-06-23 |
Family
ID=56127582
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2971268A Pending CA2971268A1 (en) | 2014-12-18 | 2015-12-17 | Detection of nucleic acid polymerase conformational changes using a nanotube |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20180051316A1 (https=) |
| EP (1) | EP3234178A4 (https=) |
| JP (1) | JP2018500905A (https=) |
| KR (1) | KR20170091158A (https=) |
| CN (2) | CN107109466A (https=) |
| AU (1) | AU2015364602B2 (https=) |
| CA (1) | CA2971268A1 (https=) |
| RU (1) | RU2721965C2 (https=) |
| SG (1) | SG11201704862UA (https=) |
| WO (2) | WO2016100637A1 (https=) |
Families Citing this family (52)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA3050062A1 (en) | 2016-01-14 | 2017-07-20 | Roswell Biotechnologies, Inc. | Molecular sensors and related methods |
| EP3408219B1 (en) | 2016-01-28 | 2022-08-17 | Roswell Biotechnologies, Inc | Massively parallel dna sequencing apparatus |
| JP7280590B2 (ja) | 2016-01-28 | 2023-05-24 | ロズウェル バイオテクノロジーズ，インコーポレイテッド | 大スケールの分子電子工学センサアレイを使用する被分析物を測定するための方法および装置 |
| US10737263B2 (en) | 2016-02-09 | 2020-08-11 | Roswell Biotechnologies, Inc. | Electronic label-free DNA and genome sequencing |
| US10597767B2 (en) | 2016-02-22 | 2020-03-24 | Roswell Biotechnologies, Inc. | Nanoparticle fabrication |
| US9829456B1 (en) | 2016-07-26 | 2017-11-28 | Roswell Biotechnologies, Inc. | Method of making a multi-electrode structure usable in molecular sensing devices |
| CN106244712B (zh) * | 2016-08-31 | 2019-11-19 | 北京大学 | Dna测序方法 |
| EP3545076A4 (en) * | 2016-11-22 | 2020-07-29 | Roswell Biotechnologies, Inc | NUCLEIC ACID SEQUENCING DEVICE CONTAINING GRAPHEN |
| WO2018132457A1 (en) | 2017-01-10 | 2018-07-19 | Roswell Biotechnologies, Inc. | Methods and systems for dna data storage |
| CN110520517A (zh) * | 2017-01-19 | 2019-11-29 | 罗斯威尔生命技术公司 | 包括二维层材料的固态测序装置 |
| US10508296B2 (en) | 2017-04-25 | 2019-12-17 | Roswell Biotechnologies, Inc. | Enzymatic circuits for molecular sensors |
| KR102879682B1 (ko) * | 2017-04-25 | 2025-10-30 | 로스웰 엠이 아이엔씨. | 분자 센서들을 위한 효소 회로들 |
| CA3057155A1 (en) | 2017-05-09 | 2018-11-15 | Roswell Biotechnologies, Inc. | Binding probe circuits for molecular sensors |
| EP3676389A4 (en) | 2017-08-30 | 2021-06-02 | Roswell Biotechnologies, Inc | PROCESSIVE ENZYMATIC MOLECULAR ELECTRONIC SENSORS FOR STORING DNA DATA |
| EP3682023B1 (en) | 2017-09-15 | 2026-01-28 | Illumina, Inc. | Tuning and calibration features of a sequence-detection system |
| WO2019055867A1 (en) * | 2017-09-15 | 2019-03-21 | Illumina, Inc. | SEQUENCE DETECTION SYSTEM |
| EP3694990A4 (en) | 2017-10-10 | 2022-06-15 | Roswell Biotechnologies, Inc. | METHODS, DEVICE AND SYSTEMS FOR AMPLIFICATION-FREE DNA DATA STORAGE |
| WO2019075419A1 (en) * | 2017-10-13 | 2019-04-18 | Dicerna Pharmaceuticals, Inc. | METHODS AND COMPOSITIONS FOR INHIBITING LDHA EXPRESSION |
| AU2019269615B2 (en) | 2018-05-17 | 2023-04-06 | Recognition AnalytiX, Inc. | Device, system and method for direct electrical measurement of enzyme activity |
| WO2020101795A1 (en) | 2018-11-15 | 2020-05-22 | Omniome, Inc. | Electronic detection of nucleic acid structure |
| EP3891304A1 (en) | 2018-12-04 | 2021-10-13 | Omniome, Inc. | Mixed-phase fluids for nucleic acid sequencing and other analytical assays |
| US12351855B2 (en) | 2019-01-30 | 2025-07-08 | Arizona Board Of Regents On Behalf Of Arizona State University | Bioelectronic circuits, systems and methods for preparing and using them |
| WO2020167574A1 (en) | 2019-02-14 | 2020-08-20 | Omniome, Inc. | Mitigating adverse impacts of detection systems on nucleic acids and other biological analytes |
| EP3931342A4 (en) * | 2019-03-01 | 2023-07-12 | Universal Sequencing Technology | BIOPOLYMER IDENTIFICATION DEVICE AND METHOD |
| CN114555832A (zh) * | 2019-05-27 | 2022-05-27 | 通用测序技术公司 | 鉴定核酸序列中组分的方法 |
| CN114728996B (zh) | 2019-09-10 | 2022-11-29 | 加利福尼亚太平洋生物科学股份有限公司 | 核苷酸的可逆修饰 |
| DE202019106694U1 (de) | 2019-12-02 | 2020-03-19 | Omniome, Inc. | System zur Sequenzierung von Nukleinsäuren in Fluidschaum |
| EP4100161B1 (en) | 2020-02-04 | 2024-07-10 | Pacific Biosciences of California, Inc. | Flow cells and methods for their use |
| US11913070B2 (en) * | 2020-02-28 | 2024-02-27 | Arizona Board Of Regents On Behalf Of Arizona State University | Methods for sequencing biopolymers |
| CN115552030A (zh) | 2020-04-17 | 2022-12-30 | 代表亚利桑那大学的亚利桑那校董事会 | 单分子电子序列检测器及其使用方法 |
| IL297732A (en) | 2020-04-30 | 2022-12-01 | Univ Arizona State | Methods for sequencing biopolymers |
| US12276653B2 (en) | 2020-05-29 | 2025-04-15 | Arizona Board Of Regents On Behalf Of Arizona State University | Bioelectronic devices with programmable adaptors |
| WO2021252800A1 (en) | 2020-06-11 | 2021-12-16 | Nautilus Biotechnology, Inc. | Methods and systems for computational decoding of biological, chemical, and physical entities |
| AU2021378290B2 (en) | 2020-11-11 | 2025-10-30 | Nautilus Subsidiary, Inc. | Affinity reagents having enhanced binding and detection characteristics |
| WO2022159663A1 (en) | 2021-01-21 | 2022-07-28 | Nautilus Biotechnology, Inc. | Systems and methods for biomolecule preparation |
| IL305336B2 (en) | 2021-03-11 | 2025-09-01 | Nautilus Subsidiary Inc | Systems and methods for preserving biomolecules |
| CA3227872A1 (en) | 2021-09-09 | 2023-03-16 | James Henry JOLY | Characterization and localization of protein modifications |
| AU2022352593B2 (en) | 2021-09-22 | 2026-03-26 | Nautilus Subsidiary, Inc. | Methods and systems for determining polypeptide interactions |
| WO2023081728A1 (en) | 2021-11-03 | 2023-05-11 | Nautilus Biotechnology, Inc. | Systems and methods for surface structuring |
| US12092578B2 (en) | 2022-03-29 | 2024-09-17 | Nautilus Subsidiary, Inc. | Integrated arrays for single-analyte processes |
| CN117174183B (zh) * | 2022-05-27 | 2025-11-25 | 中国科学院大连化学物理研究所 | 基于多尺度分子动力学模拟的rna结构预测方法 |
| US20250376716A1 (en) | 2022-06-21 | 2025-12-11 | Nautilus Subsidiary, Inc. | Method for detecting analytes at sites of optically non-resolvable distances |
| WO2024073599A1 (en) | 2022-09-29 | 2024-04-04 | Nautilus Subsidiary, Inc. | Preparation of array surfaces for single-analyte processes |
| US20240192202A1 (en) | 2022-12-09 | 2024-06-13 | Nautilus Subsidiary, Inc. | Fluidic media for single-analtye arrays |
| US20240201182A1 (en) | 2022-12-15 | 2024-06-20 | Nautilus Subsidiary, Inc. | Inhibition of photon phenomena on single molecule arrays |
| WO2024206122A1 (en) | 2023-03-24 | 2024-10-03 | Nautilus Subsidiary, Inc. | Improved transfer of nanoparticles to array surfaces |
| WO2024243237A1 (en) | 2023-05-24 | 2024-11-28 | Nautilus Subsidiary, Inc. | Systems and methods for machine vision |
| US20250066841A1 (en) | 2023-08-24 | 2025-02-27 | Nautilus Subsidiary, Inc. | Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions |
| US20250189519A1 (en) | 2023-12-11 | 2025-06-12 | Nautilus Subsidiary, Inc. | Methods and compositions of particle-based arrays |
| US20250205706A1 (en) | 2023-12-20 | 2025-06-26 | Nautilus Subsidiary, Inc. | Fluidic devices for single-analyte assays |
| US20250298016A1 (en) | 2024-03-22 | 2025-09-25 | Nautilus Subsidiary, Inc. | Plasmonic particle systems for single-analyte assays |
| US20250305034A1 (en) | 2024-04-01 | 2025-10-02 | Nautilus Subsidiary, Inc. | Nanostructures for modulation of analyte conformation |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8911972B2 (en) * | 2009-12-16 | 2014-12-16 | Pacific Biosciences Of California, Inc. | Sequencing methods using enzyme conformation |
| US20110287432A1 (en) * | 2010-05-21 | 2011-11-24 | 454 Life Science Corporation | System and method for tailoring nucleotide concentration to enzymatic efficiencies in dna sequencing technologies |
| WO2011151414A2 (en) * | 2010-06-03 | 2011-12-08 | Element Six Limited | Diamond tools |
| CN103620403B (zh) * | 2011-01-11 | 2016-08-10 | 纽约市哥伦比亚大学理事会 | 使用纳米管监测单分子的系统和方法 |
| US9164053B2 (en) * | 2011-09-26 | 2015-10-20 | The Regents Of The University Of California | Electronic device for monitoring single molecule dynamics |
| WO2013096692A1 (en) * | 2011-12-21 | 2013-06-27 | Illumina, Inc. | Apparatus and methods for kinetic analysis and determination of nucleic acid sequences |
| CN104781271B (zh) * | 2012-08-20 | 2018-07-06 | 加利福尼亚大学董事会 | 具有生物可逆的基团的多核苷酸 |
-
2015
- 2015-12-17 AU AU2015364602A patent/AU2015364602B2/en not_active Ceased
- 2015-12-17 KR KR1020177019443A patent/KR20170091158A/ko not_active Ceased
- 2015-12-17 EP EP15871053.3A patent/EP3234178A4/en not_active Withdrawn
- 2015-12-17 CN CN201580072789.4A patent/CN107109466A/zh active Pending
- 2015-12-17 WO PCT/US2015/066324 patent/WO2016100637A1/en not_active Ceased
- 2015-12-17 CA CA2971268A patent/CA2971268A1/en active Pending
- 2015-12-17 RU RU2017125417A patent/RU2721965C2/ru active
- 2015-12-17 WO PCT/US2015/066321 patent/WO2016100635A1/en not_active Ceased
- 2015-12-17 US US15/537,072 patent/US20180051316A1/en not_active Abandoned
- 2015-12-17 CN CN201811305798.5A patent/CN109517878A/zh active Pending
- 2015-12-17 JP JP2017532800A patent/JP2018500905A/ja active Pending
- 2015-12-17 SG SG11201704862UA patent/SG11201704862UA/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016100637A1 (en) | 2016-06-23 |
| WO2016100635A1 (en) | 2016-06-23 |
| RU2017125417A3 (https=) | 2019-05-27 |
| CN109517878A (zh) | 2019-03-26 |
| CN107109466A (zh) | 2017-08-29 |
| SG11201704862UA (en) | 2017-07-28 |
| RU2721965C2 (ru) | 2020-05-25 |
| AU2015364602B2 (en) | 2021-12-09 |
| RU2017125417A (ru) | 2019-01-23 |
| KR20170091158A (ko) | 2017-08-08 |
| EP3234178A4 (en) | 2018-07-11 |
| JP2018500905A (ja) | 2018-01-18 |
| AU2015364602A1 (en) | 2017-07-06 |
| EP3234178A1 (en) | 2017-10-25 |
| US20180051316A1 (en) | 2018-02-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20201204 |