CA2960902C - Biosensors for monitoring biomolecule localization and trafficking in cells - Google Patents

Biosensors for monitoring biomolecule localization and trafficking in cells Download PDF

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Publication number
CA2960902C
CA2960902C CA2960902A CA2960902A CA2960902C CA 2960902 C CA2960902 C CA 2960902C CA 2960902 A CA2960902 A CA 2960902A CA 2960902 A CA2960902 A CA 2960902A CA 2960902 C CA2960902 C CA 2960902C
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CA
Canada
Prior art keywords
biosensor
protein
targeting moiety
receptor
renilla
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CA2960902A
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English (en)
French (fr)
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CA2960902A1 (en
Inventor
Stephane Alain Laporte
Yoon NAMKUNG
Michel Bouvier
Christian Le Gouill
Mireille HOGUE
Viktoriya LUKASHEVA
Hiroyuki Kobayashi
Denis DEBLOIS
Etienne DURETTE
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite de Montreal
Royal Institution for the Advancement of Learning
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Universite de Montreal
Royal Institution for the Advancement of Learning
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Publication of CA2960902A1 publication Critical patent/CA2960902A1/en
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    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Endocrinology (AREA)
  • Zoology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Biotechnology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2960902A 2014-09-19 2015-09-21 Biosensors for monitoring biomolecule localization and trafficking in cells Active CA2960902C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462052738P 2014-09-19 2014-09-19
US62/052,738 2014-09-19
PCT/CA2015/050924 WO2016041093A1 (en) 2014-09-19 2015-09-21 Biosensors for monitoring biomolecule localization and trafficking in cells

Publications (2)

Publication Number Publication Date
CA2960902A1 CA2960902A1 (en) 2016-03-24
CA2960902C true CA2960902C (en) 2023-10-24

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2960902A Active CA2960902C (en) 2014-09-19 2015-09-21 Biosensors for monitoring biomolecule localization and trafficking in cells

Country Status (13)

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US (2) US10584159B2 (enExample)
EP (1) EP3194960B1 (enExample)
JP (1) JP6708633B2 (enExample)
CN (1) CN107533049B (enExample)
AU (1) AU2015318567B2 (enExample)
CA (1) CA2960902C (enExample)
DK (1) DK3194960T3 (enExample)
ES (1) ES2774416T3 (enExample)
IL (1) IL251158A0 (enExample)
NZ (1) NZ730131A (enExample)
PL (1) PL3194960T3 (enExample)
SG (1) SG11201702133TA (enExample)
WO (1) WO2016041093A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202001149TA (en) * 2017-10-18 2020-03-30 Univ Montreal Systems and methods for the assessment of g-protein activation
JP7631187B2 (ja) 2018-08-17 2025-02-18 ジェネンテック, インコーポレイテッド In vitroトランスサイトーシスアッセイ
JP7216216B2 (ja) * 2019-01-22 2023-01-31 インターアクス バイオテック アー・ゲー ベータ-アレスチン突然変異体
CA3144527A1 (en) 2019-08-06 2021-02-11 Domain Therapeutics 5-heteroaryl-pyridin-2-amine confounds as neuropeptide ff receptor antagonists
CN110981943B (zh) * 2019-12-02 2021-08-03 清华大学 多肽及其在制备药物中的用途和药物
WO2022108741A1 (en) * 2020-11-19 2022-05-27 University Of Houston System Monitoring membrane protein trafficking for drug discovery and drug development
CN114200128B (zh) * 2021-11-24 2024-09-27 中国科学院生物物理研究所 二维蛋白纳米阵列作为免疫传感元件载体的制备和应用
CN114767832B (zh) * 2022-04-21 2025-05-16 深圳辰扬生物科技有限公司 多肽及其用途

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7109315B2 (en) * 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
AU2002335790A1 (en) * 2001-10-15 2003-04-28 Stratagene Recombinant renilla reniformis system for bioluminescence resonance energy transfer
US7932080B2 (en) 2004-05-04 2011-04-26 Valorisation-Recherche, Limited Partnership Double brilliance beta-arrestin: a biosensor for monitoring the activity of receptors and signalling molecules, and method of using same
KR101834574B1 (ko) 2009-01-29 2018-03-05 커먼웰쓰 사이언티픽 앤드 인더스트리얼 리서치 오가니제이션 G 단백질 커플링 수용체 활성화 측정
US8795977B2 (en) * 2009-12-02 2014-08-05 Conopco, Inc. Method for screening a potential modulator compound of a taste receptor
WO2011130540A1 (en) * 2010-04-14 2011-10-20 Duke University Light stimulated protein interaction molecules and methods of use

Also Published As

Publication number Publication date
CN107533049B (zh) 2020-11-20
JP2017534851A (ja) 2017-11-24
US20170313762A1 (en) 2017-11-02
DK3194960T3 (da) 2020-02-17
AU2015318567B2 (en) 2020-11-05
CN107533049A (zh) 2018-01-02
CA2960902A1 (en) 2016-03-24
EP3194960A4 (en) 2018-03-07
US20200157184A1 (en) 2020-05-21
US10584159B2 (en) 2020-03-10
SG11201702133TA (en) 2017-04-27
ES2774416T3 (es) 2020-07-21
WO2016041093A1 (en) 2016-03-24
JP6708633B2 (ja) 2020-06-10
NZ730131A (en) 2023-05-26
EP3194960B1 (en) 2019-12-04
EP3194960A1 (en) 2017-07-26
AU2015318567A1 (en) 2017-04-06
IL251158A0 (en) 2017-04-30
PL3194960T3 (pl) 2020-06-01

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