CN107533049B - 用于监测细胞中生物分子的定位和运输的生物传感器 - Google Patents

用于监测细胞中生物分子的定位和运输的生物传感器 Download PDF

Info

Publication number
CN107533049B
CN107533049B CN201580062566.XA CN201580062566A CN107533049B CN 107533049 B CN107533049 B CN 107533049B CN 201580062566 A CN201580062566 A CN 201580062566A CN 107533049 B CN107533049 B CN 107533049B
Authority
CN
China
Prior art keywords
protein
renilla
biosensor
receptor
bret
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201580062566.XA
Other languages
English (en)
Chinese (zh)
Other versions
CN107533049A (zh
Inventor
斯特凡娜·阿兰·拉波特
允·南孔
米歇尔·布维耶
克里斯蒂安·勒古耶
米雷耶·霍格
维多利亚·卢卡舍瓦
小林弘幸
丹尼斯·德布卢瓦
艾蒂安·迪雷特
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite de Montreal
Royal Institution for the Advancement of Learning
Original Assignee
Universite de Montreal
Royal Institution for the Advancement of Learning
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universite de Montreal, Royal Institution for the Advancement of Learning filed Critical Universite de Montreal
Publication of CN107533049A publication Critical patent/CN107533049A/zh
Application granted granted Critical
Publication of CN107533049B publication Critical patent/CN107533049B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/72Receptors; Cell surface antigens; Cell surface determinants for hormones
    • C07K14/723G protein coupled receptor, e.g. TSHR-thyrotropin-receptor, LH/hCG receptor, FSH receptor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/60Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Zoology (AREA)
  • Endocrinology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201580062566.XA 2014-09-19 2015-09-21 用于监测细胞中生物分子的定位和运输的生物传感器 Active CN107533049B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462052738P 2014-09-19 2014-09-19
US62/052,738 2014-09-19
PCT/CA2015/050924 WO2016041093A1 (en) 2014-09-19 2015-09-21 Biosensors for monitoring biomolecule localization and trafficking in cells

Publications (2)

Publication Number Publication Date
CN107533049A CN107533049A (zh) 2018-01-02
CN107533049B true CN107533049B (zh) 2020-11-20

Family

ID=55532401

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201580062566.XA Active CN107533049B (zh) 2014-09-19 2015-09-21 用于监测细胞中生物分子的定位和运输的生物传感器

Country Status (13)

Country Link
US (2) US10584159B2 (enExample)
EP (1) EP3194960B1 (enExample)
JP (1) JP6708633B2 (enExample)
CN (1) CN107533049B (enExample)
AU (1) AU2015318567B2 (enExample)
CA (1) CA2960902C (enExample)
DK (1) DK3194960T3 (enExample)
ES (1) ES2774416T3 (enExample)
IL (1) IL251158A0 (enExample)
NZ (1) NZ730131A (enExample)
PL (1) PL3194960T3 (enExample)
SG (1) SG11201702133TA (enExample)
WO (1) WO2016041093A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11202001149TA (en) * 2017-10-18 2020-03-30 Univ Montreal Systems and methods for the assessment of g-protein activation
JP7631187B2 (ja) 2018-08-17 2025-02-18 ジェネンテック, インコーポレイテッド In vitroトランスサイトーシスアッセイ
JP7216216B2 (ja) * 2019-01-22 2023-01-31 インターアクス バイオテック アー・ゲー ベータ-アレスチン突然変異体
CA3144527A1 (en) 2019-08-06 2021-02-11 Domain Therapeutics 5-heteroaryl-pyridin-2-amine confounds as neuropeptide ff receptor antagonists
CN110981943B (zh) * 2019-12-02 2021-08-03 清华大学 多肽及其在制备药物中的用途和药物
WO2022108741A1 (en) * 2020-11-19 2022-05-27 University Of Houston System Monitoring membrane protein trafficking for drug discovery and drug development
CN114200128B (zh) * 2021-11-24 2024-09-27 中国科学院生物物理研究所 二维蛋白纳米阵列作为免疫传感元件载体的制备和应用
CN114767832B (zh) * 2022-04-21 2025-05-16 深圳辰扬生物科技有限公司 多肽及其用途

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7109315B2 (en) * 2000-03-15 2006-09-19 Bruce J. Bryan Renilla reniformis fluorescent proteins, nucleic acids encoding the fluorescent proteins and the use thereof in diagnostics, high throughput screening and novelty items
AU2002335790A1 (en) * 2001-10-15 2003-04-28 Stratagene Recombinant renilla reniformis system for bioluminescence resonance energy transfer
US7932080B2 (en) 2004-05-04 2011-04-26 Valorisation-Recherche, Limited Partnership Double brilliance beta-arrestin: a biosensor for monitoring the activity of receptors and signalling molecules, and method of using same
KR101834574B1 (ko) 2009-01-29 2018-03-05 커먼웰쓰 사이언티픽 앤드 인더스트리얼 리서치 오가니제이션 G 단백질 커플링 수용체 활성화 측정
US8795977B2 (en) * 2009-12-02 2014-08-05 Conopco, Inc. Method for screening a potential modulator compound of a taste receptor
WO2011130540A1 (en) * 2010-04-14 2011-10-20 Duke University Light stimulated protein interaction molecules and methods of use

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
Functional complementation of high-efficiency resonance energy transfer: a new tool for the study of protein binding interactions in living cells;Paola MOLINAR et al;《Biochemical Journal,》;20080101;第409卷(第1期);1450-1456 *
Sensitive and High Resolution Localization and Tracking of Membrane Proteins in Live Cells with BRET;Tien‐Hung Lan et al;《Traffic》;20121130;第13卷(第11期);251-261 *

Also Published As

Publication number Publication date
CA2960902C (en) 2023-10-24
JP2017534851A (ja) 2017-11-24
US20170313762A1 (en) 2017-11-02
DK3194960T3 (da) 2020-02-17
AU2015318567B2 (en) 2020-11-05
CN107533049A (zh) 2018-01-02
CA2960902A1 (en) 2016-03-24
EP3194960A4 (en) 2018-03-07
US20200157184A1 (en) 2020-05-21
US10584159B2 (en) 2020-03-10
SG11201702133TA (en) 2017-04-27
ES2774416T3 (es) 2020-07-21
WO2016041093A1 (en) 2016-03-24
JP6708633B2 (ja) 2020-06-10
NZ730131A (en) 2023-05-26
EP3194960B1 (en) 2019-12-04
EP3194960A1 (en) 2017-07-26
AU2015318567A1 (en) 2017-04-06
IL251158A0 (en) 2017-04-30
PL3194960T3 (pl) 2020-06-01

Similar Documents

Publication Publication Date Title
CN107533049B (zh) 用于监测细胞中生物分子的定位和运输的生物传感器
Drube et al. GPCR kinase knockout cells reveal the impact of individual GRKs on arrestin binding and GPCR regulation
Sauvageau et al. CNIH4 interacts with newly synthesized GPCR and controls their export from the endoplasmic reticulum
US8097422B2 (en) Kir channel modulators
Kobayashi et al. Functional rescue of β1‐adrenoceptor dimerization and trafficking by pharmacological chaperones
US9029097B2 (en) Biosensors for monitoring receptor-mediated G-protein activation
KR20140099322A (ko) 단백질 간의 상호작용의 판정 방법
Xu et al. In vivo analysis of human nucleoporin repeat domain interactions
Marsango et al. Selective phosphorylation of threonine residues defines GPR84–arrestin interactions of biased ligands
Daly et al. β-Arrestin-dependent and-independent endosomal G protein activation by the vasopressin type 2 receptor
Jang et al. Human atlastins are sufficient to drive the fusion of liposomes with a physiological lipid composition
Barki et al. Phosphorylation bar-coding of free fatty acid receptor 2 is generated in a tissue-specific manner
Mystek et al. New insights into the model of dopamine D1 receptor and G-proteins interactions
JP6192638B2 (ja) β−ガラクトシダーゼレポーターフラグメント相補性を用いるタンパク質輸送のモニタリング
US20110244487A1 (en) Methods for testing binding of a ligand to a g protein-coupled receptor
CN104781667B (zh) 荧光融合多肽、包含所述多肽的生物感应器及其用途
Frisby et al. Endosomal actin branching, fission, and receptor recycling require FCHSD2 recruitment by MICAL-L1
Drube et al. GRK2/3/5/6 knockout: The impact of individual GRKs on arrestin-binding and GPCR regulation
HK1241023B (en) Biosensors for monitoring biomolecule localization and trafficking in cells
HK1241023A1 (en) Biosensors for monitoring biomolecule localization and trafficking in cells
WO2024044308A2 (en) Materials and methods for detecting biased signaling of g-protein coupled receptors
Crilly Membrane Trafficking and Signaling of the Delta Opioid Receptor Within the Biosynthetic Pathway
US20120021440A1 (en) Methods for testing ligand binding to g protein-coupled receptors
Heydenreich The inner workings of a GPCR: molecular basis of biased G protein activation and beta-arrestin recruitment
Banushi Molecular characterisation of a novel vipar interacting protein in health and disease

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant