CA2950273C - Variants of gh family 11 xylanase and polynucleotides encoding same - Google Patents

Variants of gh family 11 xylanase and polynucleotides encoding same Download PDF

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Publication number
CA2950273C
CA2950273C CA2950273A CA2950273A CA2950273C CA 2950273 C CA2950273 C CA 2950273C CA 2950273 A CA2950273 A CA 2950273A CA 2950273 A CA2950273 A CA 2950273A CA 2950273 C CA2950273 C CA 2950273C
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CA
Canada
Prior art keywords
seq
variant
xylanase
amino acid
activity
Prior art date
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Active
Application number
CA2950273A
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English (en)
French (fr)
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CA2950273A1 (en
Inventor
James Lavigne
Brian R. Scott
Daniel Sebastian KOLCZYNSKI
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Novozymes AS
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Novozymes AS
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Publication of CA2950273A1 publication Critical patent/CA2950273A1/en
Application granted granted Critical
Publication of CA2950273C publication Critical patent/CA2950273C/en
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2482—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2950273A 2014-05-30 2015-05-22 Variants of gh family 11 xylanase and polynucleotides encoding same Active CA2950273C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462005200P 2014-05-30 2014-05-30
US62/005,200 2014-05-30
PCT/US2015/032092 WO2015183710A1 (en) 2014-05-30 2015-05-22 Variants of gh family 11 xylanase and polynucleotides encoding same

Publications (2)

Publication Number Publication Date
CA2950273A1 CA2950273A1 (en) 2015-12-03
CA2950273C true CA2950273C (en) 2022-06-21

Family

ID=53298622

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2950273A Active CA2950273C (en) 2014-05-30 2015-05-22 Variants of gh family 11 xylanase and polynucleotides encoding same

Country Status (6)

Country Link
US (1) US10385326B2 (pt)
EP (1) EP3149028B1 (pt)
BR (1) BR112016027884B1 (pt)
CA (1) CA2950273C (pt)
WO (1) WO2015183710A1 (pt)
ZA (1) ZA201608550B (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6580570B2 (ja) * 2013-09-06 2019-09-25 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 抗体安定性を向上させるための方法
JP7135334B2 (ja) * 2018-02-22 2022-09-13 株式会社豊田中央研究所 キシラナーゼ及びその利用
CN111690629B (zh) * 2020-05-29 2022-04-19 浙江工业大学 一种内切葡聚糖苷酶突变体、基因、工程菌及其应用
CN116064479B (zh) * 2022-11-30 2024-04-09 山东龙昌动物保健品股份有限公司 一种含木聚糖酶突变体和胆汁酸的复合制剂及其应用

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US5223409A (en) 1988-09-02 1993-06-29 Protein Engineering Corp. Directed evolution of novel binding proteins
DK0477280T3 (pt) 1989-06-13 1997-02-10 Genencor Int
IL97645A (en) 1990-03-23 1997-03-18 Gist Brocades Nv Production of enzymes in seeds and their use
US6395966B1 (en) 1990-08-09 2002-05-28 Dekalb Genetics Corp. Fertile transgenic maize plants containing a gene encoding the pat protein
IL99552A0 (en) 1990-09-28 1992-08-18 Ixsys Inc Compositions containing procaryotic cells,a kit for the preparation of vectors useful for the coexpression of two or more dna sequences and methods for the use thereof
DK0695349T3 (da) 1993-03-10 2004-06-01 Novozymes As Enzymer med xylanaseaktivitet stammende fra Aspergillus Aculeatus
JP2807612B2 (ja) 1993-03-12 1998-10-08 ノボ ノルディスク アクティーゼルスカブ 新規キシラナーゼ、その製造法、該キシラナーゼによるパルプ処理方法及びキシロオリゴ糖の製造法
FR2704860B1 (fr) 1993-05-05 1995-07-13 Pasteur Institut Sequences de nucleotides du locus cryiiia pour le controle de l'expression de sequences d'adn dans un hote cellulaire.
DE4343591A1 (de) 1993-12-21 1995-06-22 Evotec Biosystems Gmbh Verfahren zum evolutiven Design und Synthese funktionaler Polymere auf der Basis von Formenelementen und Formencodes
US5605793A (en) 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
KR970703426A (ko) 1994-06-03 1997-07-03 제임스 쉐한 정제된 Myceliophthora 락카제 및 그것을 암호화 하는 핵산(PURIFIED MYCELIOPHTHORA LACCASES AND NUCLEIC ACIDS ENCODING SAME)
AU2705895A (en) 1994-06-30 1996-01-25 Novo Nordisk Biotech, Inc. Non-toxic, non-toxigenic, non-pathogenic fusarium expression system and promoters and terminators for use therein
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US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
ATE332968T1 (de) 1998-10-26 2006-08-15 Novozymes As Erstellung und durchmusterung von interessierenden dna-banken in zellen von filamentösen pilzen
US7060482B1 (en) 1998-11-16 2006-06-13 National Research Council Of Canada Thermostable xylanases
EP2278016B1 (en) 1999-03-22 2012-09-26 Novozymes Inc. Promoter sequences derived from Fusarium Venenatum and uses thereof
CA2410917C (en) 2000-05-31 2012-11-27 Wing L. Sung Modified xylanases exhibiting increased thermophilicity and alkalophilicity
EP1184460A1 (en) 2000-08-29 2002-03-06 Dsm N.V. Modified fungal xylanases
US7514110B1 (en) 2000-09-21 2009-04-07 Basf Aktiengesellschaft Talaromyces xylanases
US7151204B2 (en) 2001-01-09 2006-12-19 Monsanto Technology Llc Maize chloroplast aldolase promoter compositions and methods for use thereof
JP2005500841A (ja) 2001-07-27 2005-01-13 アメリカ合衆国 オリゴヌクレオチドを用いるインビボ部位指定変異誘発のためのシステム
US7510860B1 (en) 2001-11-21 2009-03-31 National Research Council Of Canada Xylanases with enhanced thermophilicity and alkalophilicity
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CN101023173B (zh) * 2004-03-25 2012-08-08 埃欧金生物制品公司 呈现出增加表达的修饰型木聚糖酶
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WO2007115391A1 (en) 2006-04-12 2007-10-18 National Research Council Of Cananda Modification of xylanases to increase thermophilicity, thermostability and alkalophilicity
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Also Published As

Publication number Publication date
WO2015183710A1 (en) 2015-12-03
US10385326B2 (en) 2019-08-20
ZA201608550B (en) 2020-12-23
US20170101635A1 (en) 2017-04-13
BR112016027884A2 (pt) 2017-10-24
EP3149028A1 (en) 2017-04-05
CA2950273A1 (en) 2015-12-03
BR112016027884B1 (pt) 2023-05-02
EP3149028B1 (en) 2021-09-15

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