CA2941929C - Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue - Google Patents

Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue Download PDF

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Publication number
CA2941929C
CA2941929C CA2941929A CA2941929A CA2941929C CA 2941929 C CA2941929 C CA 2941929C CA 2941929 A CA2941929 A CA 2941929A CA 2941929 A CA2941929 A CA 2941929A CA 2941929 C CA2941929 C CA 2941929C
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composition
reagent
cells
chelator
tag
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CA2941929A
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English (en)
French (fr)
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CA2941929A1 (en
Inventor
Tony K. Baker
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TRUCKEE APPLIED GENOMICS LLC
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TRUCKEE APPLIED GENOMICS LLC
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10Preservation of living parts
    • A01N1/12Chemical aspects of preservation
    • A01N1/122Preservation or perfusion media
    • A01N1/126Physiologically active agents, e.g. antioxidants or nutrients
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10Preservation of living parts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Dentistry (AREA)
  • Environmental Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Plural Heterocyclic Compounds (AREA)
CA2941929A 2013-03-15 2014-03-14 Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue Active CA2941929C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361798627P 2013-03-15 2013-03-15
US61/798,627 2013-03-15
PCT/US2014/029198 WO2014144682A1 (en) 2013-03-15 2014-03-14 Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue

Publications (2)

Publication Number Publication Date
CA2941929A1 CA2941929A1 (en) 2014-09-18
CA2941929C true CA2941929C (en) 2021-02-23

Family

ID=51528685

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2941929A Active CA2941929C (en) 2013-03-15 2014-03-14 Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue

Country Status (17)

Country Link
US (5) US9113623B2 (enExample)
EP (2) EP2970878B1 (enExample)
JP (1) JP6465857B2 (enExample)
CN (2) CN112806352A (enExample)
CA (1) CA2941929C (enExample)
CY (1) CY1120801T1 (enExample)
DK (1) DK2970878T3 (enExample)
ES (1) ES2684582T3 (enExample)
HR (1) HRP20181352T1 (enExample)
HU (1) HUE039933T2 (enExample)
LT (1) LT2970878T (enExample)
PL (1) PL2970878T3 (enExample)
PT (1) PT2970878T (enExample)
RS (1) RS57900B1 (enExample)
SI (1) SI2970878T1 (enExample)
SM (1) SMT201800457T1 (enExample)
WO (1) WO2014144682A1 (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2970878B1 (en) 2013-03-15 2018-05-23 Truckee Applied Genomics, LLC Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue
EP3224359B1 (en) * 2014-11-25 2023-10-25 Bio-Rad Laboratories, Inc. Arginine improves polymerase storage stability
CN107333750A (zh) * 2017-06-11 2017-11-10 成都吱吖科技有限公司 一种长时血液细胞稳定剂
EP4089180B1 (en) 2019-09-17 2025-06-25 Longhorn Vaccines and Diagnostics, LLC Multipurpose compositions for collecting and transporting biological material
CA3185284A1 (en) * 2020-07-07 2022-01-13 Tony K. Baker Cell preservation reagents and methods of use
CN117063915B (zh) * 2023-08-17 2026-02-24 镜像绮点(上海)细胞技术有限公司 原代肿瘤细胞冻存液

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5409826A (en) * 1993-06-08 1995-04-25 Coulter Corporation Preserved, non-infectious control cells prepared by the modulation or modification of normal cells
JP2000509018A (ja) * 1996-03-26 2000-07-18 イーライ・リリー・アンド・カンパニー 肥満タンパク質製剤
US20010007673A1 (en) 1999-11-12 2001-07-12 Merrill Seymour Goldenberg Sustained-release delayed gels
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
US20080064108A1 (en) 1997-12-10 2008-03-13 Tony Baker Urine Preservation System
CA2313654A1 (en) 1997-12-10 1999-06-17 Sierra Diagnostics, Inc. Methods and reagents for preservation of dna in bodily fluids
AU1848602A (en) * 2000-12-04 2002-06-18 Lymphotec Inc. Cell-preservation liquid and method of preserving cells by using the liquid
KR20020066778A (ko) 2001-02-13 2002-08-21 한국과학기술연구원 체내 난흡수 물질의 흡수촉진용 조성물과 제형 및 그의제조방법
WO2008111981A1 (en) 2007-03-14 2008-09-18 Sierra Molecular Corporation Compositions, systems, and methods for preservation of macromolecules
WO2004052184A2 (en) * 2002-12-12 2004-06-24 Oncotech, Inc. Genes related to sensitivity and resistance to chemotherapeutic drug treatment
US8793895B2 (en) * 2006-02-10 2014-08-05 Praxair Technology, Inc. Lyophilization system and method
WO2008033936A2 (en) 2006-09-12 2008-03-20 Sierra Molecular Corporation Removal of molecular assay interferences for nucleic acids employing buffered solutions of chaotropes
US8080645B2 (en) * 2007-10-01 2011-12-20 Longhorn Vaccines & Diagnostics Llc Biological specimen collection/transport compositions and methods
US8652782B2 (en) * 2006-09-12 2014-02-18 Longhorn Vaccines & Diagnostics, Llc Compositions and methods for detecting, identifying and quantitating mycobacterial-specific nucleic acids
KR20090103867A (ko) 2006-10-27 2009-10-01 시에라 몰레큘러 코포레이션 침투가능한 격막 캡
EP2094846B8 (en) * 2006-12-13 2013-10-16 Roche Diagnostics GmbH Use of tde for the isolation of nucleic acids
EP2129780A2 (en) 2007-03-14 2009-12-09 Sierra Molecular Corporation Compositions, systems, and methods for preservation and/or stabilization of a cell and/or macromolecule
CA2705417A1 (en) * 2007-11-12 2009-05-22 Bipar Sciences, Inc. Treatment of uterine cancer and ovarian cancer with a parp inhibitor alone or in combination with anti-tumor agents
WO2010003037A2 (en) * 2008-07-03 2010-01-07 Sierra Molecular Corporation Compositions, systems, and methods for stabilization of a cell and/or macromolecule
US20110165611A1 (en) 2008-09-04 2011-07-07 Chun Li Dual modality detection of apoptosis
US9133343B2 (en) * 2009-11-30 2015-09-15 Enzo Biochem, Inc. Dyes and compositions, and processes for using same in analysis of protein aggregation and other applications
CN102318597B (zh) * 2011-08-19 2013-04-03 张雪云 一种液基细胞保存液组合物及其制备方法
EP2970878B1 (en) 2013-03-15 2018-05-23 Truckee Applied Genomics, LLC Methods and reagents for maintaining the viability of cancer cells in surgically removed tissue

Also Published As

Publication number Publication date
CN105121629B (zh) 2020-12-18
HRP20181352T1 (hr) 2018-12-14
US20150230454A1 (en) 2015-08-20
US9565852B2 (en) 2017-02-14
EP2970878B1 (en) 2018-05-23
CN105121629A (zh) 2015-12-02
US20160235055A1 (en) 2016-08-18
ES2684582T3 (es) 2018-10-03
EP3581643A1 (en) 2019-12-18
CY1120801T1 (el) 2019-12-11
US10772318B2 (en) 2020-09-15
US9949474B2 (en) 2018-04-24
PT2970878T (pt) 2018-10-09
SMT201800457T1 (it) 2018-11-09
US20140272921A1 (en) 2014-09-18
WO2014144682A1 (en) 2014-09-18
CA2941929A1 (en) 2014-09-18
LT2970878T (lt) 2018-11-12
WO2014144682A8 (en) 2015-08-13
US9113623B2 (en) 2015-08-25
PL2970878T3 (pl) 2019-01-31
RS57900B1 (sr) 2019-01-31
HUE039933T2 (hu) 2019-02-28
JP2016512693A (ja) 2016-05-09
EP2970878A1 (en) 2016-01-20
US20150327539A1 (en) 2015-11-19
JP6465857B2 (ja) 2019-02-06
HK1220481A1 (en) 2017-05-05
US20170215410A1 (en) 2017-08-03
DK2970878T3 (en) 2018-09-03
US9445586B2 (en) 2016-09-20
WO2014144682A4 (en) 2014-12-24
EP2970878A4 (en) 2016-08-03
CN112806352A (zh) 2021-05-18
SI2970878T1 (sl) 2019-02-28

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