CA2926693C - Methods for the enrichment of mutated nucleic acid from a mixture - Google Patents
Methods for the enrichment of mutated nucleic acid from a mixture Download PDFInfo
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- CA2926693C CA2926693C CA2926693A CA2926693A CA2926693C CA 2926693 C CA2926693 C CA 2926693C CA 2926693 A CA2926693 A CA 2926693A CA 2926693 A CA2926693 A CA 2926693A CA 2926693 C CA2926693 C CA 2926693C
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- Prior art keywords
- nucleic acid
- strand
- mismatch
- target nucleic
- oligonucleotide
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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| US201361909587P | 2013-11-27 | 2013-11-27 | |
| US61/909,587 | 2013-11-27 | ||
| PCT/EP2014/075460 WO2015078831A1 (en) | 2013-11-27 | 2014-11-25 | Methods for the enrichment of mutated nucleic acid from a mixture |
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| JP5795341B2 (ja) | 2010-03-08 | 2015-10-14 | デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド | 参照ブロック配列によるｆｕｌｌＣＯＬＤ−ＰＣＲ濃縮 |
| EP2691541B1 (en) | 2011-03-31 | 2017-10-18 | Dana-Farber Cancer Institute, Inc. | Method for enriching in single-stranded mutant sequences from mixture of wild-type and mutant sequences |
| AU2012242847B2 (en) | 2011-04-15 | 2017-01-19 | The Johns Hopkins University | Safe sequencing system |
| EP2912468B1 (en) | 2012-10-29 | 2018-09-12 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
| AU2016281718B2 (en) | 2015-06-24 | 2022-03-31 | Dana-Farber Cancer Institute, Inc. | Selective degradation of wild-type DNA and enrichment of mutant alleles using nuclease |
| WO2017027653A1 (en) | 2015-08-11 | 2017-02-16 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| CA3046953A1 (en) | 2016-12-12 | 2018-06-21 | Dana Farber Cancer Institute, Inc. | Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection |
| IL319255A (en) | 2017-08-07 | 2025-04-01 | Univ Johns Hopkins | Methods and materials for cancer assessment and treatment |
| US20200399683A1 (en) * | 2018-02-26 | 2020-12-24 | Cy Molecular Diagnostics, Inc. | Composition and Methods For Affinity Directed Enrichment of Rare Species |
| WO2020016590A1 (en) | 2018-07-19 | 2020-01-23 | Biofidelity Ltd | Improved polynucleotide sequence detection method |
| IL295297A (en) | 2020-02-14 | 2022-10-01 | Univ Johns Hopkins | Methods and materials for assessing nucleic acids |
| US11634754B2 (en) | 2021-04-15 | 2023-04-25 | Biofidelity Ltd. | Nucleic acid enrichment and detection |
| CN113774113B (zh) * | 2021-09-17 | 2023-07-04 | 广州医科大学附属第一医院（广州呼吸中心） | 一种富集突变基因序列的方法和应用 |
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| US6120992A (en) * | 1993-11-04 | 2000-09-19 | Valigene Corporation | Use of immobilized mismatch binding protein for detection of mutations and polymorphisms, and allele identification in a diseased human |
| US6630301B1 (en) | 1997-03-14 | 2003-10-07 | The Penn State Research Foundation | Detection of extracellular tumor-associated nucleic acid in blood plasma or serum |
| AU2324997A (en) | 1996-03-15 | 1997-10-01 | Penn State Research Foundation, The | Detection of extracellular tumor-associated nucleic acid in blood plasma or ser um using nucleic acid amplification assays |
| US6031098A (en) | 1997-08-11 | 2000-02-29 | California Institute Of Technology | Detection and treatment of duplex polynucleotide damage |
| SE9902741D0 (sv) | 1999-07-19 | 1999-07-19 | Boerries Kemper | Method for the enrichment of heteroduplexes and its use in mutation detection |
| US20030082537A1 (en) * | 2000-05-23 | 2003-05-01 | Stanton Vincent P. | Methods for genetic analysis of DNA to detect sequence variances |
| US20020132244A1 (en) | 2000-09-27 | 2002-09-19 | Xiao-Cheng Li-Sucholeiki | Methods for detecting rare polymorphic variants in genomic DNA sequences |
| WO2002057492A1 (en) * | 2000-12-08 | 2002-07-25 | California Institute Of Technology | Methods and compositions for detecting polynucleotide duplex damage |
| WO2002086196A1 (en) * | 2001-04-19 | 2002-10-31 | Rd Chemical Company | Copper acid baths, system and method for electroplating high aspect ratio substrates |
| WO2002086169A1 (en) | 2001-04-23 | 2002-10-31 | Dana-Farber Cancer Institute, Inc. | Methods for rapid screening of polymorphisms, mutations and methylation |
| GB2382137A (en) | 2001-11-20 | 2003-05-21 | Mats Gullberg | Nucleic acid enrichment |
| EP1730306A2 (en) * | 2004-03-01 | 2006-12-13 | University of Chicago | Polymorphisms in the epidermal growth factor receptor gene promoter |
| CN101370945B (zh) | 2005-10-14 | 2011-12-28 | 克利夫兰州立大学 | 用于鉴别大量野生型dna背景中多个dna突变标记的方法 |
| US20070154892A1 (en) | 2005-12-30 | 2007-07-05 | Simon Wain-Hobson | Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids |
| WO2007084380A2 (en) | 2006-01-13 | 2007-07-26 | California Institute Of Technology | Compounds and methods for nucleic acid mismatch detection |
| JP5795341B2 (ja) | 2010-03-08 | 2015-10-14 | デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド | 参照ブロック配列によるｆｕｌｌＣＯＬＤ−ＰＣＲ濃縮 |
| US20120164641A1 (en) | 2010-12-22 | 2012-06-28 | Roche Molecular Systems, Inc. | Methods and Compositions for Detecting Mutation in the Human Epidermal Growth Factor Receptor Gene |
| CN103215361A (zh) * | 2013-04-18 | 2013-07-24 | 深圳联合医学科技有限公司 | 等位基因变体检测方法、试剂盒及组合物 |
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| US20150147760A1 (en) | 2015-05-28 |
| EP3074529B1 (en) | 2018-05-02 |
| CN105745336A (zh) | 2016-07-06 |
| ES2676062T3 (es) | 2018-07-16 |
| WO2015078831A1 (en) | 2015-06-04 |
| JP2016539624A (ja) | 2016-12-22 |
| CN105745336B (zh) | 2019-03-01 |
| CA2926693A1 (en) | 2015-06-04 |
| US9873908B2 (en) | 2018-01-23 |
| EP3074529A1 (en) | 2016-10-05 |
| JP6553598B2 (ja) | 2019-07-31 |
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