CA2906231C - Microfluidic devices and methods for use thereof in multicellular assays of secretion - Google Patents

Microfluidic devices and methods for use thereof in multicellular assays of secretion Download PDF

Info

Publication number
CA2906231C
CA2906231C CA2906231A CA2906231A CA2906231C CA 2906231 C CA2906231 C CA 2906231C CA 2906231 A CA2906231 A CA 2906231A CA 2906231 A CA2906231 A CA 2906231A CA 2906231 C CA2906231 C CA 2906231C
Authority
CA
Canada
Prior art keywords
cell
readout
cells
population
effector
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2906231A
Other languages
English (en)
French (fr)
Other versions
CA2906231A1 (en
Inventor
Marketa RICICOVA
Kevin Albert HEYRIES
Hans Zahn
Oleh Petriv
Veronique Lecault
Anupam Singhal
Carl L. G. Hansen
Brad Nelson
Julie NIELSEN
Kathleen LISAINGO
Daniel J. Da Costa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of British Columbia
Original Assignee
University of British Columbia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of British Columbia filed Critical University of British Columbia
Priority to CA3234004A priority Critical patent/CA3234004A1/en
Publication of CA2906231A1 publication Critical patent/CA2906231A1/en
Application granted granted Critical
Publication of CA2906231C publication Critical patent/CA2906231C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/5052—Cells of the immune system involving B-cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502738—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by integrated valves
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
    • C07K16/1018—Orthomyxoviridae, e.g. influenza virus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/40—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0634—Cells from the blood or the immune system
    • C12N5/0635—B lymphocytes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5047—Cells of the immune system
    • G01N33/505—Cells of the immune system involving T-cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6803—General methods of protein analysis not limited to specific proteins or families of proteins
    • G01N33/6845—Methods of identifying protein-protein interactions in protein mixtures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6854—Immunoglobulins
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0099—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor comprising robots or similar manipulators
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/12—Specific details about manufacturing devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/04—Closures and closing means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • B01L2300/0838—Capillaries
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0877—Flow chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0887—Laminated structure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/14—Means for pressure control
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/70—Immunoglobulins specific features characterized by effect upon binding to a cell or to an antigen

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Dispersion Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Virology (AREA)
  • Fluid Mechanics (AREA)
  • Pulmonology (AREA)
  • Communicable Diseases (AREA)
  • Robotics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2906231A 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion Active CA2906231C (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CA3234004A CA3234004A1 (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361806329P 2013-03-28 2013-03-28
US61/806,329 2013-03-28
PCT/CA2014/000304 WO2014153651A1 (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion

Related Child Applications (1)

Application Number Title Priority Date Filing Date
CA3234004A Division CA3234004A1 (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion

Publications (2)

Publication Number Publication Date
CA2906231A1 CA2906231A1 (en) 2014-10-02
CA2906231C true CA2906231C (en) 2024-05-14

Family

ID=51622310

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2906231A Active CA2906231C (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion
CA3234004A Pending CA3234004A1 (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA3234004A Pending CA3234004A1 (en) 2013-03-28 2014-03-28 Microfluidic devices and methods for use thereof in multicellular assays of secretion

Country Status (9)

Country Link
US (3) US10725024B2 (enExample)
EP (2) EP3865212B1 (enExample)
JP (4) JP6608803B2 (enExample)
KR (3) KR102402451B1 (enExample)
CN (3) CN109342710A (enExample)
AU (3) AU2014245806B2 (enExample)
CA (2) CA2906231C (enExample)
NZ (1) NZ711723A (enExample)
WO (1) WO2014153651A1 (enExample)

Families Citing this family (126)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8691145B2 (en) 2009-11-16 2014-04-08 Flodesign Sonics, Inc. Ultrasound and acoustophoresis for water purification
US10967298B2 (en) 2012-03-15 2021-04-06 Flodesign Sonics, Inc. Driver and control for variable impedence load
US9752113B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc. Acoustic perfusion devices
US10704021B2 (en) 2012-03-15 2020-07-07 Flodesign Sonics, Inc. Acoustic perfusion devices
US9458450B2 (en) 2012-03-15 2016-10-04 Flodesign Sonics, Inc. Acoustophoretic separation technology using multi-dimensional standing waves
US9752114B2 (en) 2012-03-15 2017-09-05 Flodesign Sonics, Inc Bioreactor using acoustic standing waves
US10953436B2 (en) 2012-03-15 2021-03-23 Flodesign Sonics, Inc. Acoustophoretic device with piezoelectric transducer array
US9783775B2 (en) 2012-03-15 2017-10-10 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US10689609B2 (en) 2012-03-15 2020-06-23 Flodesign Sonics, Inc. Acoustic bioreactor processes
US10322949B2 (en) 2012-03-15 2019-06-18 Flodesign Sonics, Inc. Transducer and reflector configurations for an acoustophoretic device
US9745548B2 (en) 2012-03-15 2017-08-29 Flodesign Sonics, Inc. Acoustic perfusion devices
US9950282B2 (en) 2012-03-15 2018-04-24 Flodesign Sonics, Inc. Electronic configuration and control for acoustic standing wave generation
US9796956B2 (en) 2013-11-06 2017-10-24 Flodesign Sonics, Inc. Multi-stage acoustophoresis device
US9272234B2 (en) 2012-03-15 2016-03-01 Flodesign Sonics, Inc. Separation of multi-component fluid through ultrasonic acoustophoresis
US10370635B2 (en) 2012-03-15 2019-08-06 Flodesign Sonics, Inc. Acoustic separation of T cells
US9567559B2 (en) 2012-03-15 2017-02-14 Flodesign Sonics, Inc. Bioreactor using acoustic standing waves
US10737953B2 (en) 2012-04-20 2020-08-11 Flodesign Sonics, Inc. Acoustophoretic method for use in bioreactors
US9857333B2 (en) 2012-10-31 2018-01-02 Berkeley Lights, Inc. Pens for biological micro-objects
US9745569B2 (en) 2013-09-13 2017-08-29 Flodesign Sonics, Inc. System for generating high concentration factors for low cell density suspensions
AU2014340098B2 (en) 2013-10-22 2020-05-28 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
SG11201602629RA (en) 2013-10-22 2016-05-30 Berkeley Lights Inc Microfluidic devices having isolation pens and methods of testing biological micro-objects with same
US9889445B2 (en) 2013-10-22 2018-02-13 Berkeley Lights, Inc. Micro-fluidic devices for assaying biological activity
US11318479B2 (en) 2013-12-18 2022-05-03 Berkeley Lights, Inc. Capturing specific nucleic acid materials from individual biological cells in a micro-fluidic device
US9610579B2 (en) 2014-01-07 2017-04-04 Daktari Diagnostics, Inc. Fluid delivery devices, systems, and methods
US9725710B2 (en) 2014-01-08 2017-08-08 Flodesign Sonics, Inc. Acoustophoresis device with dual acoustophoretic chamber
US11192107B2 (en) 2014-04-25 2021-12-07 Berkeley Lights, Inc. DEP force control and electrowetting control in different sections of the same microfluidic apparatus
EP3872191B1 (en) 2014-05-22 2024-11-06 The University of British Columbia Methods for determining lymphocyte receptor chain pairs
US20150352547A1 (en) 2014-06-06 2015-12-10 Berkeley Lights, Inc. Isolating Microfluidic Structures and Trapping Bubbles
US9744483B2 (en) 2014-07-02 2017-08-29 Flodesign Sonics, Inc. Large scale acoustic separation device
WO2016073336A1 (en) * 2014-11-03 2016-05-12 Robert Etheredge Mesh microfluidic mixing chamber
EP3227684B1 (en) 2014-12-03 2019-10-02 Isoplexis Corporation Analysis and screening of cell secretion profiles
EP3610946A1 (en) 2014-12-08 2020-02-19 Berkeley Lights, Inc. Actuated microfluidic structures for directed flow in a microfluidic device and methods of use thereof cross reference to related application(s)
CN107206377B (zh) 2014-12-09 2020-02-11 伯克利之光生命科技公司 微流体装置中测定阳性的区域的自动检测
SG11201704659PA (en) 2014-12-10 2017-07-28 Berkeley Lights Inc Systems for operating electrokinetic devices
US9744533B2 (en) 2014-12-10 2017-08-29 Berkeley Lights, Inc. Movement and selection of micro-objects in a microfluidic apparatus
US11353448B2 (en) * 2015-02-13 2022-06-07 California Institute Of Technology Methods and compositions for quantifying metabolites and proteins from single cells
CN107614096A (zh) * 2015-03-13 2018-01-19 哈佛学院院长及董事 使用扩增测定细胞
US10106770B2 (en) 2015-03-24 2018-10-23 Flodesign Sonics, Inc. Methods and apparatus for particle aggregation using acoustic standing waves
US10590461B2 (en) 2015-04-21 2020-03-17 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods using magnetic beads for high throughput microbiology applications
SG11201708429WA (en) 2015-04-22 2017-11-29 Berkeley Lights Inc Microfluidic cell culture
JP2018512875A (ja) 2015-04-22 2018-05-24 バークレー ライツ，インコーポレイテッド マイクロ流体デバイスにおける細胞の凍結及び保管
WO2016172623A1 (en) 2015-04-22 2016-10-27 Berkeley Lights, Inc. Manipulation of cell nuclei in a micro-fluidic device
US10751715B1 (en) 2015-04-22 2020-08-25 Berkeley Lights, Inc. Microfluidic reporter cell assay methods and kits thereof
US11377651B2 (en) 2016-10-19 2022-07-05 Flodesign Sonics, Inc. Cell therapy processes utilizing acoustophoresis
US10640760B2 (en) 2016-05-03 2020-05-05 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
US11021699B2 (en) 2015-04-29 2021-06-01 FioDesign Sonics, Inc. Separation using angled acoustic waves
US11708572B2 (en) 2015-04-29 2023-07-25 Flodesign Sonics, Inc. Acoustic cell separation techniques and processes
US20170029802A1 (en) * 2015-07-28 2017-02-02 Flodesign Sonics, Inc. Acoustic affinity separation
US11474085B2 (en) 2015-07-28 2022-10-18 Flodesign Sonics, Inc. Expanded bed affinity selection
US11459540B2 (en) 2015-07-28 2022-10-04 Flodesign Sonics, Inc. Expanded bed affinity selection
US10799865B2 (en) 2015-10-27 2020-10-13 Berkeley Lights, Inc. Microfluidic apparatus having an optimized electrowetting surface and related systems and methods
US11666913B2 (en) 2015-11-23 2023-06-06 Berkeley Lights, Inc In situ-generated microfluidic isolation structures, kits and methods of use thereof
TWI847100B (zh) 2015-12-08 2024-07-01 美商布魯克細胞分析技術公司 在微流體器件中實施原位捕獲分析之套組
US20200149031A1 (en) 2015-12-21 2020-05-14 The University Of British Columbia Highly parallel assays for simultaneous identification of antibody sequences and binding partners
SG11201805473SA (en) 2015-12-30 2018-07-30 Berkeley Lights Inc Microfluidic devices for optically-driven convection and displacement, kits and methods thereof
WO2017117521A1 (en) 2015-12-31 2017-07-06 Berkeley Lights, Inc. Tumor infilitrating cells engineered to express a pro-inflammatory polypeptide
CN108778508B (zh) * 2016-01-14 2022-09-13 欧洲分子生物学实验室 配体诱导的细胞表达的微流体分析
WO2017123978A1 (en) 2016-01-15 2017-07-20 Berkeley Lights, Inc. Methods of producing patient-specific anti-cancer therapeutics and methods of treatment therefor
EP3411710A1 (en) 2016-02-05 2018-12-12 The Broad Institute Inc. Multi-stage, multiplexed target isolation and processing from heterogeneous populations
EP3429753A4 (en) 2016-03-16 2019-11-06 Berkeley Lights, Inc. METHODS, SYSTEMS AND DEVICES FOR SELECTION AND PRODUCTION OF GENERICALLY WORKED CLONES
WO2017156634A1 (en) * 2016-03-17 2017-09-21 The University Of British Columbia Devices and methods for cellular secretion analysis
CA3020976A1 (en) 2016-04-15 2017-10-19 Berkeley Lights, Inc. Methods, systems and kits for in-pen assays
US10675625B2 (en) 2016-04-15 2020-06-09 Berkeley Lights, Inc Light sequencing and patterns for dielectrophoretic transport
US10940476B2 (en) 2016-04-22 2021-03-09 Arizona Board Of Regents On Behalf Of Arizona State University Device for high-throughput multi-parameter functional profiling of the same cells in multicellular settings and in isolation
US10710006B2 (en) 2016-04-25 2020-07-14 Flodesign Sonics, Inc. Piezoelectric transducer for generation of an acoustic standing wave
US11214789B2 (en) 2016-05-03 2022-01-04 Flodesign Sonics, Inc. Concentration and washing of particles with acoustics
US11085035B2 (en) 2016-05-03 2021-08-10 Flodesign Sonics, Inc. Therapeutic cell washing, concentration, and separation utilizing acoustophoresis
SG10202008265XA (en) 2016-05-26 2020-09-29 Berkeley Lights Inc Covalently modified surfaces, kits, and methods of preparation and use
EP3478416A4 (en) * 2016-06-30 2019-12-18 General Automation Lab Technologies, Inc. HIGH-RESOLUTION SYSTEMS, KITS, DEVICE AND METHOD USING MAGNETIC BALLS FOR MICROBIOLOGICAL APPLICATIONS WITH HIGH THROUGHPUT
SG11201900442PA (en) 2016-07-21 2019-02-27 Berkeley Lights Inc Sorting of t lymphocytes in a microfluidic device
EP3510398A1 (en) 2016-09-12 2019-07-17 Isoplexis Corporation System and methods for multiplexed analysis of cellular and other immunotherapeutics
EP3512945A4 (en) 2016-09-15 2020-08-26 Augmenta Bioworks, Inc. METHODS AND APPLICATIONS OF AMPLIFICATION OF IMMUNOLOGICAL DIRECTORY SEQUENCES
JP2020513248A (ja) 2016-10-19 2020-05-14 フロデザイン ソニックス， インク．Ｆｌｏｄｅｓｉｇｎ Ｓｏｎｉｃｓ， Ｉｎｃ． 音響による親和性細胞抽出
CN106520537B (zh) * 2016-10-21 2018-10-23 南通大学 一种t细胞免疫应答的微流控光学分析系统及分析方法
US11493508B2 (en) 2016-11-11 2022-11-08 IsoPlexis Corporation Compositions and methods for the simultaneous genomic, transcriptomic and proteomic analysis of single cells
EP3552223A4 (en) * 2016-12-06 2020-11-11 Brandeis University FREEZING FLUID CELL FOR CRYO-ELECTRONIC MICROSCOPY
WO2018126205A1 (en) * 2016-12-30 2018-07-05 The Regents Of The University Of California Methods for selection and generation of genome edited t cells
CN118846017A (zh) 2017-07-21 2024-10-29 伯克利之光生命科技公司 抗原呈递合成表面、共价官能化表面、活化t细胞及其用途
US11478798B2 (en) * 2017-10-02 2022-10-25 Khalifa University of Science and Technology Microfluidic device for generating an in vitro lymph node
CN111182971B (zh) * 2017-10-03 2022-08-30 阿威尔斯医疗公司 用于基于氧化还原反应测定微生物的浓度和微生物对抗感染剂的敏感性的装置、系统和方法
WO2019075476A2 (en) 2017-10-15 2019-04-18 Berkeley Lights, Inc. METHODS, SYSTEMS AND KITS FOR ENCLOSED TESTS
US11760965B2 (en) 2017-10-19 2023-09-19 Georgia Tech Research Corporation Mesofluidic device for culture of cell aggregates
US10785574B2 (en) 2017-12-14 2020-09-22 Flodesign Sonics, Inc. Acoustic transducer driver and controller
US12122988B2 (en) * 2018-03-12 2024-10-22 Sony Corporation Cell evaluation device and cell evaluation system
EP3765069A1 (en) 2018-03-16 2021-01-20 Zoetis Services LLC Peptide vaccines against interleukin-31
CA3093539A1 (en) 2018-03-16 2019-09-19 Zoetis Services Llc Interleukin-31 monoclonal antibodies for veterinary use
WO2019191321A1 (en) * 2018-03-28 2019-10-03 10X Genomics, Inc. Nucleic acid enrichment within partitions
WO2019185874A1 (en) * 2018-03-30 2019-10-03 Exosomics S.p.A. Use of hollow fibers to obtain blood or a blood derivative impoverished from blood cells and platelets derived extracellular vesicles
US20210054324A1 (en) * 2018-04-05 2021-02-25 University Of Virginia Patent Foundation 3d spatially patterned lymph node on a microfluidic device
US11666914B2 (en) * 2018-05-09 2023-06-06 Tecan Trading Ag Cartridge, electrowetting sample processing system and bead manipulation method
CN110499251A (zh) * 2018-05-17 2019-11-26 中国科学院大连化学物理研究所 高通量多参数的单细胞源性细胞外囊泡分析芯片及应用
CA3112333A1 (en) * 2018-09-14 2020-03-19 Berkeley Lights, Inc. Methods for assaying binding affinity
AU2019343994A1 (en) 2018-09-21 2021-05-13 Berkeley Lights, Inc. Functionalized well plate, methods of preparation and use thereof
WO2020077017A2 (en) 2018-10-10 2020-04-16 Augmenta Bioworks, Inc. Methods for isolating immune binding proteins
EP3863757A4 (en) 2018-10-11 2022-07-20 Berkeley Lights, Inc. SYSTEMS AND METHODS FOR IDENTIFICATION OF OPTIMIZED PROTEIN PRODUCTION AND KITS THEREFORE
CN109576345A (zh) * 2018-10-17 2019-04-05 西人马（厦门）科技有限公司 一种用于dna提取的微流控芯片及其检测方法
US20200139362A1 (en) 2018-10-18 2020-05-07 Berkeley Lights, Inc. Proto-Antigen-Presenting Synthetic Surfaces, Activated T Cells, and Uses Thereof
CA3118957A1 (en) * 2018-11-01 2020-05-07 Berkeley Lights, Inc. Methods for assaying biological cells in a microfluidic environment
GB201820617D0 (en) * 2018-12-18 2019-01-30 Greindx As System for capturing cells
EP3670667A1 (en) * 2018-12-19 2020-06-24 Paris Sciences et Lettres - Quartier Latin Identification of cognate pairs of ligands and receptors
WO2020168258A1 (en) 2019-02-15 2020-08-20 Berkeley Lights, Inc. Laser-assisted repositioning of a micro-object and culturing of an attachment-dependent cell in a microfluidic environment
US20200300861A1 (en) * 2019-03-22 2020-09-24 Augmenta Bioworks, Inc. Isolation of Single Cells and Uses Thereof
CN110006866B (zh) * 2019-04-16 2022-04-22 杭州迈尔德生物科技有限公司 一种阿片类活性物质的通用检测方法及其检测试剂盒
EP3962652A4 (en) 2019-04-30 2023-01-18 Berkeley Lights, Inc. CELL ENCAPSULATION AND ANALYSIS PROCESSES
CN116157134A (zh) 2019-05-28 2023-05-23 希里斯公司 用于患者来源的微器官球的方法和装置
US20200392578A1 (en) * 2019-06-14 2020-12-17 The Regents Of The University Of California Methods of sequencing antibody chains from hybridomas and kits for practicing same
TWI884171B (zh) * 2019-09-30 2025-05-21 日商東京應化工業股份有限公司 分泌物產生細胞的篩選方法、及分泌物產生細胞的篩選套組
WO2021097456A1 (en) * 2019-11-17 2021-05-20 The Regents Of The University Of California Guided Microfluidic Flow for Cell Capture, Indexing, and Directed Release
AU2020388676B2 (en) * 2019-11-21 2024-07-18 Emulate Inc. Antibody producing microfluidic devices
CN112557475B (zh) * 2020-02-19 2021-07-06 南京启医科技有限公司 一种基于微流控和elisa分析的外泌体分离检测系统
CN113373104B (zh) * 2020-03-09 2022-11-18 浙江大学 一种用于稀有细胞高纯度分选的装置及方法
CN114410466A (zh) * 2020-04-01 2022-04-29 沈西凌 用于个性化癌症治疗的精确药物筛选
CN113529075B (zh) * 2020-04-20 2022-05-03 厦门大学 一种液态金属复合多孔膜及其制备方法和应用
US20230182135A1 (en) * 2020-05-14 2023-06-15 Bifrost Biosystems A microfluidic device having specifically designed detection chambers
TWI729914B (zh) * 2020-08-18 2021-06-01 國立臺灣海洋大學 鮮度指示裝置
EP4454659A3 (en) 2020-08-18 2025-01-22 Cephalon LLC Anti-par-2 antibodies and methods of use thereof
NL2026383B1 (en) * 2020-08-31 2022-04-29 Lumicks Ca Holding B V Method and system for studying objects, in particular biological cells
EP4337260A4 (en) * 2021-05-14 2025-01-29 The Texas A&M University System PLATFORM FOR FAST, LABEL-FREE, AUTOMATED ASSESSMENT OF STERILITY AND BIOCONTAMINATION
WO2023225668A1 (en) * 2022-05-19 2023-11-23 Triplebar Bio, Inc. Multiparametric discovery and optimization platform
CN115735846B (zh) * 2022-11-29 2025-09-16 复旦大学附属华山医院 磁场联合光化学诱导无创性缺血性脑卒中模型的建立方法
WO2024130036A1 (en) * 2022-12-14 2024-06-20 President And Fellows Of Harvard College Systems and methods for identifying gpcr modulators and other agents
KR102543112B1 (ko) * 2023-04-12 2023-06-13 주식회사 브레디스헬스케어 유체 시료 내에 극미량으로 존재하는 분석 대상 물질의 입자를 검출하기 위한 방법
CN116626293A (zh) * 2023-06-08 2023-08-22 复旦大学 一种用于检测溶组织内阿米巴感染的微流控芯片
WO2025111584A1 (en) * 2023-11-22 2025-05-30 Georgia Tech Research Corporation An electronic microfluidic platform for on-chip apoptosis quantification using annexin v-based ps externalization detection
WO2025182763A1 (ja) * 2024-02-29 2025-09-04 富士レビオ株式会社 検出方法、検出装置、並びに、これらに用いる処理液及びキット
CN118731352B (zh) * 2024-09-02 2024-12-06 天津锐尔康生物科技有限公司 用于细胞表面标志物的自动化分析检测方法及系统

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008010A (en) * 1996-11-01 1999-12-28 University Of Pittsburgh Method and apparatus for holding cells
DE19725190A1 (de) * 1997-06-14 1998-12-17 Innova Gmbh Vorrichtungen mit integrierten Elektroden aus elektrisch leitfähigen Kunststoffen
US6740497B2 (en) * 1998-03-06 2004-05-25 The Regents Of The University Of California Method and apparatus for detecting cancerous cells using molecules that change electrophoretic mobility
WO2004040000A2 (en) 2002-09-09 2004-05-13 Nura, Inc G protein coupled receptors and uses thereof
TWI382843B (zh) * 2004-10-07 2013-01-21 Argos Therapeutics Inc 成熟樹突細胞組合物及培養彼等之方法
WO2006060748A2 (en) 2004-12-03 2006-06-08 California Institute Of Technology Microfluidic sieve valves
US8796020B2 (en) * 2005-06-17 2014-08-05 Inha-Industry Partnership Institute Manufacturing process for fresh and frozen stem cells
US20100249022A1 (en) * 2007-05-18 2010-09-30 University Of Massachusetts Strategy for cloning and expressing the extracellular domains of receptors as soluble proteins
EP2271657B1 (en) * 2008-03-04 2017-03-01 Crystal Bioscience Inc. Gel microdrop composition and method of using the same
WO2010005593A1 (en) * 2008-07-11 2010-01-14 President And Fellows Of Harvard College Systems and methods of droplet-based selection
GB2464300A (en) 2008-10-10 2010-04-14 Univ Dublin City Microfluidic multiplexed cellular and molecular analysis device and method
US10087408B2 (en) 2010-07-07 2018-10-02 The University Of British Columbia System and method for microfluidic cell culture
US9188593B2 (en) * 2010-07-16 2015-11-17 The University Of British Columbia Methods for assaying cellular binding interactions
EP2646798B1 (en) 2010-12-03 2017-06-28 Cellply S.R.L. Microanalysis of cellular function
CA2836608C (en) 2011-05-27 2016-10-11 The University Of British Columbia Microfluidic cell trap and assay apparatus for high-throughput analysis
US9250229B2 (en) * 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis

Also Published As

Publication number Publication date
KR102257305B1 (ko) 2021-05-28
KR102668221B1 (ko) 2024-05-23
JP2024020238A (ja) 2024-02-14
EP2978855A1 (en) 2016-02-03
WO2014153651A8 (en) 2014-11-06
AU2022202333B2 (en) 2024-12-19
NZ711723A (en) 2021-12-24
CA3234004A1 (en) 2014-10-02
KR20220070579A (ko) 2022-05-31
AU2014245806A1 (en) 2015-09-24
JP2021078503A (ja) 2021-05-27
JP6608803B2 (ja) 2019-11-20
AU2020200414A1 (en) 2020-02-13
CN115074413A (zh) 2022-09-20
US20200363401A1 (en) 2020-11-19
EP2978855B1 (en) 2021-01-13
JP7374936B2 (ja) 2023-11-07
KR20150134429A (ko) 2015-12-01
AU2014245806B2 (en) 2019-10-31
JP7699639B2 (ja) 2025-06-27
KR20210063450A (ko) 2021-06-01
WO2014153651A9 (en) 2016-03-10
AU2022202333A1 (en) 2022-04-28
AU2020200414B2 (en) 2022-04-14
CA2906231A1 (en) 2014-10-02
US10725024B2 (en) 2020-07-28
WO2014153651A1 (en) 2014-10-02
CN105492621A (zh) 2016-04-13
AU2014245806A9 (en) 2016-06-16
US20160252495A1 (en) 2016-09-01
EP2978855A4 (en) 2016-10-26
EP3865212A1 (en) 2021-08-18
JP2020043856A (ja) 2020-03-26
JP2016515823A (ja) 2016-06-02
US20220365072A1 (en) 2022-11-17
KR102402451B1 (ko) 2022-05-26
JP6825065B2 (ja) 2021-02-03
CN109342710A (zh) 2019-02-15
EP3865212B1 (en) 2024-03-13

Similar Documents

Publication Publication Date Title
US20220365072A1 (en) Microfluidic Devices and Methods for Use Thereof in Multicellular Assays of Secretion
AU2023208186A1 (en) Devices and methods for cellular secretion analysis
HK40058018A (en) Microfluidic devices and methods for use thereof in multicellular assays of secretion

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20190107

EEER Examination request

Effective date: 20190107

EEER Examination request

Effective date: 20190107

EEER Examination request

Effective date: 20190107

EEER Examination request

Effective date: 20190107

EEER Examination request

Effective date: 20190107

EEER Examination request

Effective date: 20190107

EEER Examination request

Effective date: 20190107

EEER Examination request

Effective date: 20190107

EEER Examination request

Effective date: 20190107

EEER Examination request

Effective date: 20190107