CA2900005A1 - Fungal resistant plants expressing mybtf - Google Patents
Fungal resistant plants expressing mybtf Download PDFInfo
- Publication number
- CA2900005A1 CA2900005A1 CA2900005A CA2900005A CA2900005A1 CA 2900005 A1 CA2900005 A1 CA 2900005A1 CA 2900005 A CA2900005 A CA 2900005A CA 2900005 A CA2900005 A CA 2900005A CA 2900005 A1 CA2900005 A1 CA 2900005A1
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- plant
- protein
- mybtf
- seq
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000002538 fungal effect Effects 0.000 title claims description 119
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 229
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 178
- 238000000034 method Methods 0.000 claims abstract description 144
- 230000009261 transgenic effect Effects 0.000 claims abstract description 141
- 239000012634 fragment Substances 0.000 claims abstract description 120
- 230000001965 increasing effect Effects 0.000 claims abstract description 85
- 230000014509 gene expression Effects 0.000 claims abstract description 81
- 241000196324 Embryophyta Species 0.000 claims description 612
- 150000007523 nucleic acids Chemical class 0.000 claims description 585
- 102000039446 nucleic acids Human genes 0.000 claims description 548
- 108020004707 nucleic acids Proteins 0.000 claims description 548
- 235000010469 Glycine max Nutrition 0.000 claims description 99
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 86
- 244000068988 Glycine max Species 0.000 claims description 85
- 239000013598 vector Substances 0.000 claims description 65
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 59
- 230000002068 genetic effect Effects 0.000 claims description 59
- 230000000295 complement effect Effects 0.000 claims description 48
- 230000009466 transformation Effects 0.000 claims description 41
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 39
- 241000682645 Phakopsora pachyrhizi Species 0.000 claims description 31
- 240000008042 Zea mays Species 0.000 claims description 29
- 241000233866 Fungi Species 0.000 claims description 25
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 25
- 238000004519 manufacturing process Methods 0.000 claims description 24
- 241000440445 Phakopsora meibomiae Species 0.000 claims description 19
- 235000021307 Triticum Nutrition 0.000 claims description 15
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 14
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 14
- 240000004713 Pisum sativum Species 0.000 claims description 14
- 240000005979 Hordeum vulgare Species 0.000 claims description 13
- 235000010582 Pisum sativum Nutrition 0.000 claims description 13
- 240000004658 Medicago sativa Species 0.000 claims description 12
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 12
- 239000002253 acid Substances 0.000 claims description 11
- 230000000694 effects Effects 0.000 claims description 10
- 230000001131 transforming effect Effects 0.000 claims description 10
- 240000007594 Oryza sativa Species 0.000 claims description 9
- 235000007164 Oryza sativa Nutrition 0.000 claims description 9
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 9
- 235000005822 corn Nutrition 0.000 claims description 9
- 235000009566 rice Nutrition 0.000 claims description 8
- 241000219194 Arabidopsis Species 0.000 claims description 7
- 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 claims description 7
- 244000043158 Lens esculenta Species 0.000 claims description 7
- 241000233679 Peronosporaceae Species 0.000 claims description 7
- 241000219793 Trifolium Species 0.000 claims description 7
- 210000002615 epidermis Anatomy 0.000 claims description 7
- 244000105624 Arachis hypogaea Species 0.000 claims description 6
- 235000010777 Arachis hypogaea Nutrition 0.000 claims description 6
- 241000221785 Erysiphales Species 0.000 claims description 6
- 241000219745 Lupinus Species 0.000 claims description 6
- 235000010624 Medicago sativa Nutrition 0.000 claims description 6
- 244000046146 Pueraria lobata Species 0.000 claims description 6
- 235000010575 Pueraria lobata Nutrition 0.000 claims description 6
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 6
- 244000061456 Solanum tuberosum Species 0.000 claims description 6
- 241000219873 Vicia Species 0.000 claims description 6
- 230000005030 transcription termination Effects 0.000 claims description 6
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 claims description 5
- 150000007513 acids Chemical class 0.000 claims description 5
- 238000009395 breeding Methods 0.000 claims description 5
- 230000001488 breeding effect Effects 0.000 claims description 5
- -1 preferably Substances 0.000 claims description 5
- 230000001172 regenerating effect Effects 0.000 claims description 5
- 235000003276 Apios tuberosa Nutrition 0.000 claims description 4
- 235000010744 Arachis villosulicarpa Nutrition 0.000 claims description 4
- 235000006008 Brassica napus var napus Nutrition 0.000 claims description 4
- 235000019764 Soybean Meal Nutrition 0.000 claims description 4
- 239000003921 oil Substances 0.000 claims description 4
- 235000019198 oils Nutrition 0.000 claims description 4
- 239000004455 soybean meal Substances 0.000 claims description 4
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 240000000111 Saccharum officinarum Species 0.000 claims description 3
- 235000007201 Saccharum officinarum Nutrition 0.000 claims description 3
- 241001533598 Septoria Species 0.000 claims description 3
- 238000003306 harvesting Methods 0.000 claims description 3
- 235000012424 soybean oil Nutrition 0.000 claims description 3
- 239000003549 soybean oil Substances 0.000 claims description 3
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 claims description 2
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 claims description 2
- 240000000385 Brassica napus var. napus Species 0.000 claims description 2
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 claims description 2
- 241000219146 Gossypium Species 0.000 claims description 2
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 2
- 235000021536 Sugar beet Nutrition 0.000 claims description 2
- 235000012054 meals Nutrition 0.000 claims description 2
- 235000012015 potatoes Nutrition 0.000 claims description 2
- 240000004322 Lens culinaris Species 0.000 claims 1
- 240000008790 Musa x paradisiaca Species 0.000 claims 1
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 244000052769 pathogen Species 0.000 abstract description 59
- 244000053095 fungal pathogen Species 0.000 abstract description 47
- 241000221535 Pucciniales Species 0.000 abstract description 23
- 238000003259 recombinant expression Methods 0.000 abstract description 11
- 239000013604 expression vector Substances 0.000 abstract description 7
- 101710197341 Myb-like transcription factor Proteins 0.000 description 330
- 235000018102 proteins Nutrition 0.000 description 153
- 210000004027 cell Anatomy 0.000 description 131
- 108090000765 processed proteins & peptides Proteins 0.000 description 89
- 102000004196 processed proteins & peptides Human genes 0.000 description 88
- 229920001184 polypeptide Polymers 0.000 description 80
- 239000002609 medium Substances 0.000 description 38
- 241000589158 Agrobacterium Species 0.000 description 36
- 210000001519 tissue Anatomy 0.000 description 34
- 230000001717 pathogenic effect Effects 0.000 description 32
- 108091028043 Nucleic acid sequence Proteins 0.000 description 31
- 108020004414 DNA Proteins 0.000 description 30
- 125000003729 nucleotide group Chemical group 0.000 description 30
- 239000002773 nucleotide Substances 0.000 description 29
- 239000000047 product Substances 0.000 description 28
- 238000006467 substitution reaction Methods 0.000 description 22
- 150000001413 amino acids Chemical class 0.000 description 21
- 238000009396 hybridization Methods 0.000 description 21
- 201000010099 disease Diseases 0.000 description 20
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 20
- 125000000539 amino acid group Chemical group 0.000 description 18
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 16
- 235000009973 maize Nutrition 0.000 description 16
- 208000015181 infectious disease Diseases 0.000 description 15
- 241000209140 Triticum Species 0.000 description 14
- 230000004071 biological effect Effects 0.000 description 13
- 238000011081 inoculation Methods 0.000 description 13
- 239000013612 plasmid Substances 0.000 description 13
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 12
- 210000002257 embryonic structure Anatomy 0.000 description 12
- 235000007238 Secale cereale Nutrition 0.000 description 11
- 230000003993 interaction Effects 0.000 description 11
- 241000223218 Fusarium Species 0.000 description 10
- 241000209056 Secale Species 0.000 description 10
- 230000006870 function Effects 0.000 description 10
- 238000002360 preparation method Methods 0.000 description 10
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 9
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 9
- 230000012010 growth Effects 0.000 description 9
- 210000000056 organ Anatomy 0.000 description 9
- 230000002018 overexpression Effects 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 238000013518 transcription Methods 0.000 description 9
- 230000035897 transcription Effects 0.000 description 9
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 8
- 238000012217 deletion Methods 0.000 description 8
- 230000037430 deletion Effects 0.000 description 8
- 239000003550 marker Substances 0.000 description 8
- 230000001404 mediated effect Effects 0.000 description 8
- 230000000644 propagated effect Effects 0.000 description 8
- 230000008929 regeneration Effects 0.000 description 8
- 238000011069 regeneration method Methods 0.000 description 8
- 238000012216 screening Methods 0.000 description 8
- 239000007787 solid Substances 0.000 description 8
- 238000012546 transfer Methods 0.000 description 8
- 238000004422 calculation algorithm Methods 0.000 description 7
- 239000002299 complementary DNA Substances 0.000 description 7
- 238000003780 insertion Methods 0.000 description 7
- 230000037431 insertion Effects 0.000 description 7
- 239000007788 liquid Substances 0.000 description 7
- 239000000463 material Substances 0.000 description 7
- 238000011426 transformation method Methods 0.000 description 7
- 229920001817 Agar Polymers 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 6
- 108091005461 Nucleic proteins Proteins 0.000 description 6
- 241001123583 Puccinia striiformis Species 0.000 description 6
- 229920002494 Zein Polymers 0.000 description 6
- 239000008272 agar Substances 0.000 description 6
- 238000004458 analytical method Methods 0.000 description 6
- 230000007123 defense Effects 0.000 description 6
- 238000011161 development Methods 0.000 description 6
- 230000018109 developmental process Effects 0.000 description 6
- 230000006698 induction Effects 0.000 description 6
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 6
- 230000014616 translation Effects 0.000 description 6
- 108700026215 vpr Genes Proteins 0.000 description 6
- 239000005019 zein Substances 0.000 description 6
- 229940093612 zein Drugs 0.000 description 6
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 5
- 241000190150 Bipolaris sorokiniana Species 0.000 description 5
- 241000228439 Bipolaris zeicola Species 0.000 description 5
- 241001429695 Colletotrichum graminicola Species 0.000 description 5
- 102000053602 DNA Human genes 0.000 description 5
- 241000282414 Homo sapiens Species 0.000 description 5
- 206010020649 Hyperkeratosis Diseases 0.000 description 5
- 108091092195 Intron Proteins 0.000 description 5
- NWBJYWHLCVSVIJ-UHFFFAOYSA-N N-benzyladenine Chemical compound N=1C=NC=2NC=NC=2C=1NCC1=CC=CC=C1 NWBJYWHLCVSVIJ-UHFFFAOYSA-N 0.000 description 5
- 241000208317 Petroselinum Species 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 235000011197 perejil Nutrition 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 5
- 230000010076 replication Effects 0.000 description 5
- 241000894007 species Species 0.000 description 5
- 239000000725 suspension Substances 0.000 description 5
- 238000003786 synthesis reaction Methods 0.000 description 5
- 238000013519 translation Methods 0.000 description 5
- 238000005406 washing Methods 0.000 description 5
- XOSXWYQMOYSSKB-LDKJGXKFSA-L water blue Chemical compound CC1=CC(/C(\C(C=C2)=CC=C2NC(C=C2)=CC=C2S([O-])(=O)=O)=C(\C=C2)/C=C/C\2=N\C(C=C2)=CC=C2S([O-])(=O)=O)=CC(S(O)(=O)=O)=C1N.[Na+].[Na+] XOSXWYQMOYSSKB-LDKJGXKFSA-L 0.000 description 5
- 241001530056 Athelia rolfsii Species 0.000 description 4
- 241001161772 Bipolaris rostrata Species 0.000 description 4
- 244000045232 Canavalia ensiformis Species 0.000 description 4
- 108020004635 Complementary DNA Proteins 0.000 description 4
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 4
- 241000223195 Fusarium graminearum Species 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 4
- 241000209504 Poaceae Species 0.000 description 4
- 241000332749 Setosphaeria turcica Species 0.000 description 4
- 240000003768 Solanum lycopersicum Species 0.000 description 4
- 240000006677 Vicia faba Species 0.000 description 4
- 235000014787 Vitis vinifera Nutrition 0.000 description 4
- 240000006365 Vitis vinifera Species 0.000 description 4
- OJOBTAOGJIWAGB-UHFFFAOYSA-N acetosyringone Chemical compound COC1=CC(C(C)=O)=CC(OC)=C1O OJOBTAOGJIWAGB-UHFFFAOYSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000003795 chemical substances by application Substances 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 238000005520 cutting process Methods 0.000 description 4
- 230000001419 dependent effect Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 4
- 239000003623 enhancer Substances 0.000 description 4
- 230000005714 functional activity Effects 0.000 description 4
- 230000001939 inductive effect Effects 0.000 description 4
- 230000000442 meristematic effect Effects 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 4
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000035764 nutrition Effects 0.000 description 4
- 230000003032 phytopathogenic effect Effects 0.000 description 4
- 230000008488 polyadenylation Effects 0.000 description 4
- 102000040430 polynucleotide Human genes 0.000 description 4
- 108091033319 polynucleotide Proteins 0.000 description 4
- 239000002157 polynucleotide Substances 0.000 description 4
- 229940070376 protein Drugs 0.000 description 4
- 238000010186 staining Methods 0.000 description 4
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 3
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000221198 Basidiomycota Species 0.000 description 3
- 241000335053 Beta vulgaris Species 0.000 description 3
- 241000121260 Bipolaris victoriae Species 0.000 description 3
- 241001480061 Blumeria graminis Species 0.000 description 3
- 240000002791 Brassica napus Species 0.000 description 3
- 235000010520 Canavalia ensiformis Nutrition 0.000 description 3
- 108091026890 Coding region Proteins 0.000 description 3
- 241001040638 Curvularia eragrostidis Species 0.000 description 3
- 241000935926 Diplodia Species 0.000 description 3
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 3
- 108090000790 Enzymes Proteins 0.000 description 3
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 description 3
- 239000005977 Ethylene Substances 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- XVOKUMIPKHGGTN-UHFFFAOYSA-N Imazethapyr Chemical compound OC(=O)C1=CC(CC)=CN=C1C1=NC(C)(C(C)C)C(=O)N1 XVOKUMIPKHGGTN-UHFFFAOYSA-N 0.000 description 3
- 235000010671 Lathyrus sativus Nutrition 0.000 description 3
- 240000005783 Lathyrus sativus Species 0.000 description 3
- 244000061176 Nicotiana tabacum Species 0.000 description 3
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 241000233654 Oomycetes Species 0.000 description 3
- 108700001094 Plant Genes Proteins 0.000 description 3
- 235000010580 Psophocarpus tetragonolobus Nutrition 0.000 description 3
- 244000046095 Psophocarpus tetragonolobus Species 0.000 description 3
- 241001123561 Puccinia coronata Species 0.000 description 3
- 241000221301 Puccinia graminis Species 0.000 description 3
- 241001123569 Puccinia recondita Species 0.000 description 3
- 241001123567 Puccinia sorghi Species 0.000 description 3
- 241001183191 Sclerophthora macrospora Species 0.000 description 3
- 101000959886 Solanum tuberosum Aspartic protease inhibitor 2 Proteins 0.000 description 3
- 101000959868 Solanum tuberosum Aspartic protease inhibitor 8 Proteins 0.000 description 3
- 108700019146 Transgenes Proteins 0.000 description 3
- 241000221577 Uromyces appendiculatus Species 0.000 description 3
- 235000010749 Vicia faba Nutrition 0.000 description 3
- 244000042295 Vigna mungo Species 0.000 description 3
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 238000003556 assay Methods 0.000 description 3
- 230000001580 bacterial effect Effects 0.000 description 3
- 230000031018 biological processes and functions Effects 0.000 description 3
- 210000000349 chromosome Anatomy 0.000 description 3
- 239000004062 cytokinin Substances 0.000 description 3
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 3
- 235000005489 dwarf bean Nutrition 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000005755 formation reaction Methods 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 238000000338 in vitro Methods 0.000 description 3
- ZNJFBWYDHIGLCU-HWKXXFMVSA-N jasmonic acid Chemical compound CC\C=C/C[C@@H]1[C@@H](CC(O)=O)CCC1=O ZNJFBWYDHIGLCU-HWKXXFMVSA-N 0.000 description 3
- 238000000386 microscopy Methods 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 238000002887 multiple sequence alignment Methods 0.000 description 3
- 108010058731 nopaline synthase Proteins 0.000 description 3
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 230000035515 penetration Effects 0.000 description 3
- 239000012071 phase Substances 0.000 description 3
- 239000013600 plasmid vector Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 108091008146 restriction endonucleases Proteins 0.000 description 3
- 238000003757 reverse transcription PCR Methods 0.000 description 3
- 229960004889 salicylic acid Drugs 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 238000002741 site-directed mutagenesis Methods 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000013603 viral vector Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 238000004383 yellowing Methods 0.000 description 3
- CAAMSDWKXXPUJR-UHFFFAOYSA-N 3,5-dihydro-4H-imidazol-4-one Chemical compound O=C1CNC=N1 CAAMSDWKXXPUJR-UHFFFAOYSA-N 0.000 description 2
- 102000007469 Actins Human genes 0.000 description 2
- 108010085238 Actins Proteins 0.000 description 2
- 241000213004 Alternaria solani Species 0.000 description 2
- 241000235349 Ascomycota Species 0.000 description 2
- 235000007319 Avena orientalis Nutrition 0.000 description 2
- 244000075850 Avena orientalis Species 0.000 description 2
- 244000105627 Cajanus indicus Species 0.000 description 2
- 235000010773 Cajanus indicus Nutrition 0.000 description 2
- 229920000018 Callose Polymers 0.000 description 2
- 241001330709 Cochliobolus pallescens Species 0.000 description 2
- 241000222199 Colletotrichum Species 0.000 description 2
- 241000371648 Curvularia intermedia Species 0.000 description 2
- 241000072225 Curvularia tuberculata Species 0.000 description 2
- 244000303965 Cyamopsis psoralioides Species 0.000 description 2
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 2
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 2
- 241000555695 Didymella Species 0.000 description 2
- 241001422851 Didymella maydis Species 0.000 description 2
- 241000306559 Exserohilum Species 0.000 description 2
- 241000223194 Fusarium culmorum Species 0.000 description 2
- 108010068370 Glutens Proteins 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 235000008694 Humulus lupulus Nutrition 0.000 description 2
- 244000025221 Humulus lupulus Species 0.000 description 2
- 206010020751 Hypersensitivity Diseases 0.000 description 2
- 206010061217 Infestation Diseases 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 description 2
- 244000112675 Lablab purpureus Species 0.000 description 2
- 241000228457 Leptosphaeria maculans Species 0.000 description 2
- 241001495426 Macrophomina phaseolina Species 0.000 description 2
- 241000221574 Melampsora lini Species 0.000 description 2
- 208000031888 Mycoses Diseases 0.000 description 2
- 208000012868 Overgrowth Diseases 0.000 description 2
- 241000760719 Peronosclerospora maydis Species 0.000 description 2
- 241001183114 Peronosclerospora sacchari Species 0.000 description 2
- 241000596141 Peronosclerospora sorghi Species 0.000 description 2
- 241001223281 Peronospora Species 0.000 description 2
- 244000309475 Phaeosphaeria maydis Species 0.000 description 2
- 241001307099 Phakopsoraceae Species 0.000 description 2
- 240000001956 Phaseolus acutifolius Species 0.000 description 2
- 235000010632 Phaseolus coccineus Nutrition 0.000 description 2
- 235000010617 Phaseolus lunatus Nutrition 0.000 description 2
- 244000042209 Phaseolus multiflorus Species 0.000 description 2
- 241000233622 Phytophthora infestans Species 0.000 description 2
- 241000219843 Pisum Species 0.000 description 2
- 235000015622 Pisum sativum var macrocarpon Nutrition 0.000 description 2
- 241001403501 Pisum sativum var. macrocarpum Species 0.000 description 2
- 102400001018 Proadrenomedullin N-20 terminal peptide Human genes 0.000 description 2
- 101800000795 Proadrenomedullin N-20 terminal peptide Proteins 0.000 description 2
- 241000520648 Pyrenophora teres Species 0.000 description 2
- 241000233639 Pythium Species 0.000 description 2
- 108020004511 Recombinant DNA Proteins 0.000 description 2
- 241001361634 Rhizoctonia Species 0.000 description 2
- 241000813090 Rhizoctonia solani Species 0.000 description 2
- 241000342317 Sclerospora Species 0.000 description 2
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 2
- 241000986481 Selenophoma Species 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 244000062793 Sorghum vulgare Species 0.000 description 2
- 235000011989 Sphenostylis stenocarpa Nutrition 0.000 description 2
- 244000213793 Sphenostylis stenocarpa Species 0.000 description 2
- 241000533281 Stagonospora Species 0.000 description 2
- 241000692746 Stenocarpella maydis Species 0.000 description 2
- 241000724803 Sugarcane bacilliform virus Species 0.000 description 2
- 108020005038 Terminator Codon Proteins 0.000 description 2
- 108090000848 Ubiquitin Proteins 0.000 description 2
- 102000044159 Ubiquitin Human genes 0.000 description 2
- 241000221576 Uromyces Species 0.000 description 2
- 235000002096 Vicia faba var. equina Nutrition 0.000 description 2
- 241000219977 Vigna Species 0.000 description 2
- 235000010725 Vigna aconitifolia Nutrition 0.000 description 2
- 244000042325 Vigna aconitifolia Species 0.000 description 2
- 235000010711 Vigna angularis Nutrition 0.000 description 2
- 240000007098 Vigna angularis Species 0.000 description 2
- 235000010716 Vigna mungo Nutrition 0.000 description 2
- 240000004922 Vigna radiata Species 0.000 description 2
- 235000011453 Vigna umbellata Nutrition 0.000 description 2
- 244000042314 Vigna unguiculata Species 0.000 description 2
- 206010052428 Wound Diseases 0.000 description 2
- 208000027418 Wounds and injury Diseases 0.000 description 2
- 241001360088 Zymoseptoria tritici Species 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 244000000060 biotrophic pathogen Species 0.000 description 2
- 239000003610 charcoal Substances 0.000 description 2
- 229940106265 charcoal Drugs 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 244000038559 crop plants Species 0.000 description 2
- 230000003412 degenerative effect Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 235000021186 dishes Nutrition 0.000 description 2
- 101150059880 dsdA gene Proteins 0.000 description 2
- 239000012877 elongation medium Substances 0.000 description 2
- 229940108890 emend Drugs 0.000 description 2
- 230000002708 enhancing effect Effects 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000006052 feed supplement Substances 0.000 description 2
- 238000007429 general method Methods 0.000 description 2
- 235000007924 ground bean Nutrition 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 230000007246 mechanism Effects 0.000 description 2
- 238000002844 melting Methods 0.000 description 2
- 230000008018 melting Effects 0.000 description 2
- 210000000473 mesophyll cell Anatomy 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000002703 mutagenesis Methods 0.000 description 2
- 231100000350 mutagenesis Toxicity 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000007899 nucleic acid hybridization Methods 0.000 description 2
- 230000005305 organ development Effects 0.000 description 2
- 229930195732 phytohormone Natural products 0.000 description 2
- 230000037039 plant physiology Effects 0.000 description 2
- 230000010152 pollination Effects 0.000 description 2
- 230000004224 protection Effects 0.000 description 2
- 210000001938 protoplast Anatomy 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 108020003175 receptors Proteins 0.000 description 2
- 102000005962 receptors Human genes 0.000 description 2
- 230000006798 recombination Effects 0.000 description 2
- 238000005215 recombination Methods 0.000 description 2
- 230000003362 replicative effect Effects 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 238000001228 spectrum Methods 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 230000002103 transcriptional effect Effects 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- CLQMBPJKHLGMQK-UHFFFAOYSA-N 2-(4-isopropyl-4-methyl-5-oxo-4,5-dihydro-1H-imidazol-2-yl)nicotinic acid Chemical compound N1C(=O)C(C(C)C)(C)N=C1C1=NC=CC=C1C(O)=O CLQMBPJKHLGMQK-UHFFFAOYSA-N 0.000 description 1
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid Chemical compound CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 1
- 108020005345 3' Untranslated Regions Proteins 0.000 description 1
- 101150073246 AGL1 gene Proteins 0.000 description 1
- 108010000700 Acetolactate synthase Proteins 0.000 description 1
- 101710197633 Actin-1 Proteins 0.000 description 1
- 241000919511 Albugo Species 0.000 description 1
- 244000291564 Allium cepa Species 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 241000223602 Alternaria alternata Species 0.000 description 1
- 241001149961 Alternaria brassicae Species 0.000 description 1
- 235000010585 Ammi visnaga Nutrition 0.000 description 1
- 244000153158 Ammi visnaga Species 0.000 description 1
- 101710117679 Anthocyanidin 3-O-glucosyltransferase Proteins 0.000 description 1
- 244000105975 Antidesma platyphyllum Species 0.000 description 1
- 240000001436 Antirrhinum majus Species 0.000 description 1
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 101100175474 Arabidopsis thaliana GLP4 gene Proteins 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000222195 Ascochyta Species 0.000 description 1
- 244000309474 Ascochyta maydis Species 0.000 description 1
- 244000309473 Ascochyta tritici Species 0.000 description 1
- 244000309488 Ascochyta zeicola Species 0.000 description 1
- 244000003416 Asparagus officinalis Species 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 108010023063 Bacto-peptone Proteins 0.000 description 1
- 101000972350 Bombyx mori Lebocin-4 Proteins 0.000 description 1
- 241000123650 Botrytis cinerea Species 0.000 description 1
- 235000011331 Brassica Nutrition 0.000 description 1
- 241000219198 Brassica Species 0.000 description 1
- 240000007124 Brassica oleracea Species 0.000 description 1
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 1
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 1
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 1
- 241000233685 Bremia lactucae Species 0.000 description 1
- 229910000906 Bronze Inorganic materials 0.000 description 1
- 241000220451 Canavalia Species 0.000 description 1
- 235000010518 Canavalia gladiata Nutrition 0.000 description 1
- 241001107116 Castanospermum australe Species 0.000 description 1
- 241000701489 Cauliflower mosaic virus Species 0.000 description 1
- 241000530549 Cercospora beticola Species 0.000 description 1
- 241001157784 Cercospora nicotianae Species 0.000 description 1
- 206010068051 Chimerism Diseases 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 108010022172 Chitinases Proteins 0.000 description 1
- 102000012286 Chitinases Human genes 0.000 description 1
- KZBUYRJDOAKODT-UHFFFAOYSA-N Chlorine Chemical compound ClCl KZBUYRJDOAKODT-UHFFFAOYSA-N 0.000 description 1
- 241000760356 Chytridiomycetes Species 0.000 description 1
- 235000010521 Cicer Nutrition 0.000 description 1
- 241000220455 Cicer Species 0.000 description 1
- 235000010523 Cicer arietinum Nutrition 0.000 description 1
- 244000045195 Cicer arietinum Species 0.000 description 1
- 108010061190 Cinnamyl-alcohol dehydrogenase Proteins 0.000 description 1
- 241000221751 Claviceps purpurea Species 0.000 description 1
- 108020004705 Codon Proteins 0.000 description 1
- 241000626656 Colletotrichum falcatum Species 0.000 description 1
- 241001120669 Colletotrichum lindemuthianum Species 0.000 description 1
- 241001123530 Cronartium Species 0.000 description 1
- 241001123528 Cronartium ribicola Species 0.000 description 1
- 241000223208 Curvularia Species 0.000 description 1
- 241000371652 Curvularia clavata Species 0.000 description 1
- 241001537312 Curvularia inaequalis Species 0.000 description 1
- 241000223211 Curvularia lunata Species 0.000 description 1
- 241001586664 Curvularia senegalensis Species 0.000 description 1
- 229920000832 Cutin Polymers 0.000 description 1
- 235000008222 Cyamopsis psoralioides Nutrition 0.000 description 1
- MTCFGRXMJLQNBG-UWTATZPHSA-N D-Serine Chemical compound OC[C@@H](N)C(O)=O MTCFGRXMJLQNBG-UWTATZPHSA-N 0.000 description 1
- 229930195711 D-Serine Natural products 0.000 description 1
- 102000012410 DNA Ligases Human genes 0.000 description 1
- 108010061982 DNA Ligases Proteins 0.000 description 1
- 102000004594 DNA Polymerase I Human genes 0.000 description 1
- 108010017826 DNA Polymerase I Proteins 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 206010011878 Deafness Diseases 0.000 description 1
- 235000007007 Dolichos lablab Nutrition 0.000 description 1
- 241001057636 Dracaena deremensis Species 0.000 description 1
- 241001506775 Epicoccum nigrum Species 0.000 description 1
- 241000510928 Erysiphe necator Species 0.000 description 1
- 241001489205 Erysiphe pisi Species 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- 241000701484 Figwort mosaic virus Species 0.000 description 1
- 108010040721 Flagellin Proteins 0.000 description 1
- 235000016623 Fragaria vesca Nutrition 0.000 description 1
- 240000009088 Fragaria x ananassa Species 0.000 description 1
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 1
- 206010017533 Fungal infection Diseases 0.000 description 1
- 208000004770 Fusariosis Diseases 0.000 description 1
- 241000122692 Fusarium avenaceum Species 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241000690372 Fusarium proliferatum Species 0.000 description 1
- 241000526118 Fusarium solani f. radicicola Species 0.000 description 1
- 241000145502 Fusarium subglutinans Species 0.000 description 1
- 241000233732 Fusarium verticillioides Species 0.000 description 1
- 241001149475 Gaeumannomyces graminis Species 0.000 description 1
- 241000702463 Geminiviridae Species 0.000 description 1
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 1
- 241000204888 Geobacter sp. Species 0.000 description 1
- 241001315191 Gladiata Species 0.000 description 1
- 108010061711 Gliadin Proteins 0.000 description 1
- 101710186901 Globulin 1 Proteins 0.000 description 1
- 241001620302 Glomerella <beetle> Species 0.000 description 1
- 239000005561 Glufosinate Substances 0.000 description 1
- 102100037473 Glutathione S-transferase A1 Human genes 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241000947775 Graphium penicillioides Species 0.000 description 1
- 241000221557 Gymnosporangium Species 0.000 description 1
- 241001516047 Gymnosporangium juniperi-virginianae Species 0.000 description 1
- 244000020551 Helianthus annuus Species 0.000 description 1
- 235000003222 Helianthus annuus Nutrition 0.000 description 1
- 241001181537 Hemileia Species 0.000 description 1
- 241001181532 Hemileia vastatrix Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101001026125 Homo sapiens Glutathione S-transferase A1 Proteins 0.000 description 1
- 241000721662 Juniperus Species 0.000 description 1
- FAIXYKHYOGVFKA-UHFFFAOYSA-N Kinetin Natural products N=1C=NC=2N=CNC=2C=1N(C)C1=CC=CO1 FAIXYKHYOGVFKA-UHFFFAOYSA-N 0.000 description 1
- 239000004201 L-cysteine Substances 0.000 description 1
- 235000013878 L-cysteine Nutrition 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 241000219729 Lathyrus Species 0.000 description 1
- 235000007252 Lathyrus tuberosus Nutrition 0.000 description 1
- 244000237786 Lathyrus tuberosus Species 0.000 description 1
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 1
- 101710094902 Legumin Proteins 0.000 description 1
- 241000219739 Lens Species 0.000 description 1
- 235000010666 Lens esculenta Nutrition 0.000 description 1
- 244000309489 Leptosphaeria maydis Species 0.000 description 1
- 244000309491 Leptothyrium zeae Species 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 239000006142 Luria-Bertani Agar Substances 0.000 description 1
- 241000584607 Macrospora Species 0.000 description 1
- 235000012549 Macrotyloma uniflorum Nutrition 0.000 description 1
- 244000131099 Macrotyloma uniflorum Species 0.000 description 1
- 241001344131 Magnaporthe grisea Species 0.000 description 1
- 241000219823 Medicago Species 0.000 description 1
- 206010027146 Melanoderma Diseases 0.000 description 1
- 241001022799 Microdochium sorghi Species 0.000 description 1
- 241001459558 Monographella nivalis Species 0.000 description 1
- 101100170937 Mus musculus Dnmt1 gene Proteins 0.000 description 1
- 240000005561 Musa balbisiana Species 0.000 description 1
- 101710202365 Napin Proteins 0.000 description 1
- 241001226034 Nectria <echinoderm> Species 0.000 description 1
- 241000556984 Neonectria galligena Species 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 239000004677 Nylon Substances 0.000 description 1
- 241001668536 Oculimacula yallundae Species 0.000 description 1
- 102000015636 Oligopeptides Human genes 0.000 description 1
- 108010038807 Oligopeptides Proteins 0.000 description 1
- 241001465313 Ophiosphaerella herpotricha Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241001560086 Pachyrhizus Species 0.000 description 1
- 235000001591 Pachyrhizus erosus Nutrition 0.000 description 1
- 244000258470 Pachyrhizus tuberosus Species 0.000 description 1
- 235000018669 Pachyrhizus tuberosus Nutrition 0.000 description 1
- 241000572009 Paraphaeosphaeria michotii Species 0.000 description 1
- 241000228143 Penicillium Species 0.000 description 1
- 235000002233 Penicillium roqueforti Nutrition 0.000 description 1
- 102000002508 Peptide Elongation Factors Human genes 0.000 description 1
- 108010068204 Peptide Elongation Factors Proteins 0.000 description 1
- 241000596140 Peronosclerospora Species 0.000 description 1
- 241000760727 Peronosclerospora philippinensis Species 0.000 description 1
- 241001627691 Peronospora antirrhini Species 0.000 description 1
- 241001670201 Peronospora destructor Species 0.000 description 1
- 241000118319 Peronospora effusa Species 0.000 description 1
- 241000582441 Peronospora tabacina Species 0.000 description 1
- 240000009164 Petroselinum crispum Species 0.000 description 1
- 235000002770 Petroselinum crispum Nutrition 0.000 description 1
- 241000555275 Phaeosphaeria Species 0.000 description 1
- 241000440444 Phakopsora Species 0.000 description 1
- 241000219833 Phaseolus Species 0.000 description 1
- 235000007848 Phaseolus acutifolius Nutrition 0.000 description 1
- 235000008527 Phaseolus acutifolius var tenuifolius Nutrition 0.000 description 1
- 235000006089 Phaseolus angularis Nutrition 0.000 description 1
- 244000100170 Phaseolus lunatus Species 0.000 description 1
- 241000975369 Phoma betae Species 0.000 description 1
- 241001207509 Phoma sp. Species 0.000 description 1
- 241001557902 Phomopsis sp. Species 0.000 description 1
- 102100033118 Phosphatidate cytidylyltransferase 1 Human genes 0.000 description 1
- 101710178747 Phosphatidate cytidylyltransferase 1 Proteins 0.000 description 1
- 102100033126 Phosphatidate cytidylyltransferase 2 Human genes 0.000 description 1
- 101710178746 Phosphatidate cytidylyltransferase 2 Proteins 0.000 description 1
- 108091000041 Phosphoenolpyruvate Carboxylase Proteins 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 244000309487 Physopella pallescens Species 0.000 description 1
- 241000233614 Phytophthora Species 0.000 description 1
- 241000233620 Phytophthora cryptogea Species 0.000 description 1
- 241001503436 Plasmodiophora brassicae Species 0.000 description 1
- 241001503460 Plasmodiophorida Species 0.000 description 1
- 241000233626 Plasmopara Species 0.000 description 1
- 241000233610 Plasmopara halstedii Species 0.000 description 1
- 241001281803 Plasmopara viticola Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 108010059820 Polygalacturonase Proteins 0.000 description 1
- 241001219485 Polymyxa graminis Species 0.000 description 1
- 241000709992 Potato virus X Species 0.000 description 1
- 244000028344 Primula vulgaris Species 0.000 description 1
- 235000016311 Primula vulgaris Nutrition 0.000 description 1
- 241000342307 Pseudoperonospora humuli Species 0.000 description 1
- 241001480433 Pseudopeziza Species 0.000 description 1
- 241001480435 Pseudopeziza medicaginis Species 0.000 description 1
- 241000221300 Puccinia Species 0.000 description 1
- 241000567197 Puccinia graminis f. sp. tritici Species 0.000 description 1
- 241001141507 Puccinia hemerocallidis Species 0.000 description 1
- 241001620046 Puccinia persistens Species 0.000 description 1
- 241001304534 Puccinia polysora Species 0.000 description 1
- 241000352029 Pucciniomycetes Species 0.000 description 1
- 241000228453 Pyrenophora Species 0.000 description 1
- 241000228454 Pyrenophora graminea Species 0.000 description 1
- 241000190117 Pyrenophora tritici-repentis Species 0.000 description 1
- 241000599030 Pythium debaryanum Species 0.000 description 1
- 101100437728 Rattus norvegicus Bloc1s2 gene Proteins 0.000 description 1
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 1
- 241000701507 Rice tungro bacilliform virus Species 0.000 description 1
- 244000004774 Sabina virginiana Species 0.000 description 1
- 235000008691 Sabina virginiana Nutrition 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 244000309484 Sclerophthora rayssiae Species 0.000 description 1
- 241000342322 Sclerospora graminicola Species 0.000 description 1
- 241001558929 Sclerotium <basidiomycota> Species 0.000 description 1
- 244000309478 Septoria zeae Species 0.000 description 1
- 244000309476 Septoria zeicola Species 0.000 description 1
- 244000309477 Septoria zeina Species 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 235000002634 Solanum Nutrition 0.000 description 1
- 241000207763 Solanum Species 0.000 description 1
- 241001250060 Sphacelotheca Species 0.000 description 1
- 235000009337 Spinacia oleracea Nutrition 0.000 description 1
- 244000300264 Spinacia oleracea Species 0.000 description 1
- 241001219481 Spongospora subterranea Species 0.000 description 1
- 241001250070 Sporisorium reilianum Species 0.000 description 1
- 241000116011 Stenocarpella macrospora Species 0.000 description 1
- 241000723873 Tobacco mosaic virus Species 0.000 description 1
- 241000723573 Tobacco rattle virus Species 0.000 description 1
- 108091023040 Transcription factor Proteins 0.000 description 1
- 102000040945 Transcription factor Human genes 0.000 description 1
- 235000015724 Trifolium pratense Nutrition 0.000 description 1
- 235000013540 Trifolium repens var repens Nutrition 0.000 description 1
- 235000004240 Triticum spelta Nutrition 0.000 description 1
- 241000959260 Typhula Species 0.000 description 1
- 241000333201 Typhula incarnata Species 0.000 description 1
- 108091023045 Untranslated Region Proteins 0.000 description 1
- 241000514371 Ustilago avenae Species 0.000 description 1
- 235000015919 Ustilago maydis Nutrition 0.000 description 1
- 244000301083 Ustilago maydis Species 0.000 description 1
- 241000007070 Ustilago nuda Species 0.000 description 1
- 241000233791 Ustilago tritici Species 0.000 description 1
- 241000228452 Venturia inaequalis Species 0.000 description 1
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 1
- 241001123668 Verticillium dahliae Species 0.000 description 1
- 235000002098 Vicia faba var. major Nutrition 0.000 description 1
- 244000007839 Vigna catjang Species 0.000 description 1
- 235000010500 Vigna catjang Nutrition 0.000 description 1
- 235000006085 Vigna mungo var mungo Nutrition 0.000 description 1
- 235000006582 Vigna radiata Nutrition 0.000 description 1
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 1
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 1
- 235000010726 Vigna sinensis Nutrition 0.000 description 1
- 240000001417 Vigna umbellata Species 0.000 description 1
- 235000010722 Vigna unguiculata Nutrition 0.000 description 1
- 235000005745 Vigna unguiculata ssp. cylindrica Nutrition 0.000 description 1
- 235000011408 Vigna vexillata Nutrition 0.000 description 1
- 244000136476 Vigna vexillata Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 235000013030 Voandzeia subterranea Nutrition 0.000 description 1
- 244000170226 Voandzeia subterranea Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 235000007244 Zea mays Nutrition 0.000 description 1
- 101001036768 Zea mays Glucose-1-phosphate adenylyltransferase large subunit 1, chloroplastic/amyloplastic Proteins 0.000 description 1
- 101000662549 Zea mays Sucrose synthase 1 Proteins 0.000 description 1
- HEYSFDAMRDTCJM-UUOKFMHZSA-N [(2r,3s,4r,5r)-5-(2-amino-6-oxo-3h-purin-9-yl)-4-hydroxy-3-phosphonooxyoxolan-2-yl]methyl phosphono hydrogen phosphate Chemical compound C1=2NC(N)=NC(=O)C=2N=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H]1O HEYSFDAMRDTCJM-UUOKFMHZSA-N 0.000 description 1
- 241000845448 [Rhizoctonia] zeae Species 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 244000000025 aggressive pathogen Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 238000003782 apoptosis assay Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000000680 avirulence Effects 0.000 description 1
- 210000003578 bacterial chromosome Anatomy 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 101150103518 bar gene Proteins 0.000 description 1
- 102000023732 binding proteins Human genes 0.000 description 1
- 108091008324 binding proteins Proteins 0.000 description 1
- 235000021279 black bean Nutrition 0.000 description 1
- 239000007844 bleaching agent Substances 0.000 description 1
- 239000010974 bronze Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 229940077731 carbohydrate nutrients Drugs 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 108010040093 cellulose synthase Proteins 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 229930002868 chlorophyll a Natural products 0.000 description 1
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 1
- 229930002869 chlorophyll b Natural products 0.000 description 1
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 238000003776 cleavage reaction Methods 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 239000012881 co-culture medium Substances 0.000 description 1
- 229940000425 combination drug Drugs 0.000 description 1
- 238000009833 condensation Methods 0.000 description 1
- 230000005494 condensation Effects 0.000 description 1
- KUNSUQLRTQLHQQ-UHFFFAOYSA-N copper tin Chemical compound [Cu].[Sn] KUNSUQLRTQLHQQ-UHFFFAOYSA-N 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 210000000172 cytosol Anatomy 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- NEKNNCABDXGBEN-UHFFFAOYSA-L disodium;4-(4-chloro-2-methylphenoxy)butanoate;4-(2,4-dichlorophenoxy)butanoate Chemical compound [Na+].[Na+].CC1=CC(Cl)=CC=C1OCCCC([O-])=O.[O-]C(=O)CCCOC1=CC=C(Cl)C=C1Cl NEKNNCABDXGBEN-UHFFFAOYSA-L 0.000 description 1
- MOTZDAYCYVMXPC-UHFFFAOYSA-N dodecyl hydrogen sulfate Chemical compound CCCCCCCCCCCCOS(O)(=O)=O MOTZDAYCYVMXPC-UHFFFAOYSA-N 0.000 description 1
- 229940043264 dodecyl sulfate Drugs 0.000 description 1
- 244000013123 dwarf bean Species 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000012636 effector Substances 0.000 description 1
- 238000004520 electroporation Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 210000001339 epidermal cell Anatomy 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 210000001723 extracellular space Anatomy 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
- 235000019197 fats Nutrition 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 108010050792 glutenin Proteins 0.000 description 1
- 235000009424 haa Nutrition 0.000 description 1
- 244000000061 hemibiotrophic pathogen Species 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000012405 in silico analysis Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 239000012499 inoculation medium Substances 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 230000002147 killing effect Effects 0.000 description 1
- QANMHLXAZMSUEX-UHFFFAOYSA-N kinetin Chemical compound N=1C=NC=2N=CNC=2C=1NCC1=CC=CO1 QANMHLXAZMSUEX-UHFFFAOYSA-N 0.000 description 1
- 229960001669 kinetin Drugs 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 108010083942 mannopine synthase Proteins 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- MCPLVIGCWWTHFH-UHFFFAOYSA-L methyl blue Chemical group [Na+].[Na+].C1=CC(S(=O)(=O)[O-])=CC=C1NC1=CC=C(C(=C2C=CC(C=C2)=[NH+]C=2C=CC(=CC=2)S([O-])(=O)=O)C=2C=CC(NC=3C=CC(=CC=3)S([O-])(=O)=O)=CC=2)C=C1 MCPLVIGCWWTHFH-UHFFFAOYSA-L 0.000 description 1
- 238000000520 microinjection Methods 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000009343 monoculture Methods 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229920001778 nylon Polymers 0.000 description 1
- 244000000177 oomycete pathogen Species 0.000 description 1
- 108090000021 oryzin Proteins 0.000 description 1
- 230000007170 pathology Effects 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000010647 peptide synthesis reaction Methods 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- NONJJLVGHLVQQM-JHXYUMNGSA-N phenethicillin Chemical compound N([C@@H]1C(N2[C@H](C(C)(C)S[C@@H]21)C(O)=O)=O)C(=O)C(C)OC1=CC=CC=C1 NONJJLVGHLVQQM-JHXYUMNGSA-N 0.000 description 1
- 150000002989 phenols Chemical class 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 230000008659 phytopathology Effects 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 210000000745 plant chromosome Anatomy 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 229940036310 program Drugs 0.000 description 1
- 230000005522 programmed cell death Effects 0.000 description 1
- 108060006613 prolamin Proteins 0.000 description 1
- 230000004952 protein activity Effects 0.000 description 1
- 230000004853 protein function Effects 0.000 description 1
- 238000001243 protein synthesis Methods 0.000 description 1
- 230000036647 reaction Effects 0.000 description 1
- 235000013526 red clover Nutrition 0.000 description 1
- 230000002829 reductive effect Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000008261 resistance mechanism Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 239000012882 rooting medium Substances 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 230000007017 scission Effects 0.000 description 1
- 238000013077 scoring method Methods 0.000 description 1
- 230000008117 seed development Effects 0.000 description 1
- 230000007226 seed germination Effects 0.000 description 1
- 239000006152 selective media Substances 0.000 description 1
- 238000002864 sequence alignment Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 230000011869 shoot development Effects 0.000 description 1
- 229960005076 sodium hypochlorite Drugs 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 108010048090 soybean lectin Proteins 0.000 description 1
- 229960000268 spectinomycin Drugs 0.000 description 1
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000007480 spreading Effects 0.000 description 1
- 238000003892 spreading Methods 0.000 description 1
- 239000012192 staining solution Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 235000008070 tepary bean Nutrition 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 102000027257 transmembrane receptors Human genes 0.000 description 1
- 108091008578 transmembrane receptors Proteins 0.000 description 1
- 108040005733 triose-phosphate:phosphate antiporter activity proteins Proteins 0.000 description 1
- 238000013022 venting Methods 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000007923 virulence factor Effects 0.000 description 1
- 239000000304 virulence factor Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
- 238000001262 western blot Methods 0.000 description 1
- 230000003245 working effect Effects 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8282—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for fungal resistance
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP13158321.3 | 2013-03-08 | ||
| EP13158321 | 2013-03-08 | ||
| PCT/EP2014/054461 WO2014135682A1 (en) | 2013-03-08 | 2014-03-07 | Fungal resistant plants expressing mybtf |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2900005A1 true CA2900005A1 (en) | 2014-09-12 |
Family
ID=47827076
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2900005A Abandoned CA2900005A1 (en) | 2013-03-08 | 2014-03-07 | Fungal resistant plants expressing mybtf |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US10465204B2 (https=) |
| CN (1) | CN105008542B (https=) |
| AR (1) | AR096014A1 (https=) |
| BR (1) | BR112015021857B1 (https=) |
| CA (1) | CA2900005A1 (https=) |
| WO (1) | WO2014135682A1 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014117988A1 (en) | 2013-01-29 | 2014-08-07 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing hcp7 |
| US10435705B2 (en) | 2013-01-29 | 2019-10-08 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing HCP6 |
| WO2014118018A1 (en) | 2013-01-29 | 2014-08-07 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing ein2 |
| WO2014135682A1 (en) | 2013-03-08 | 2014-09-12 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing mybtf |
| BR112017016688B1 (pt) | 2015-02-04 | 2024-01-23 | Basf Plant Science Company Gmbh | Método para aumentar a resistência fúngica, método para a produção de um produto e método para criar uma planta resistente a fungos |
| CN106754960B (zh) | 2016-12-20 | 2019-07-23 | 南京农业大学 | 一个nlr类基因nlr1-v及其表达载体和应用 |
| CN106939328A (zh) * | 2017-03-01 | 2017-07-11 | 浙江省萧山棉麻研究所 | 一种快速筛选培育抗锈病病菌的萱草种质的方法 |
| CN107236746B (zh) * | 2017-07-28 | 2020-03-24 | 福建农林大学 | 小麦白粉病抗性基因PmR2及其克隆与应用 |
| CN112239763B (zh) * | 2020-10-18 | 2022-03-11 | 华中农业大学 | OsMYB63基因在改良水稻抗病性中的应用 |
| CN117757803B (zh) * | 2023-11-22 | 2025-10-31 | 福建省农业科学院果树研究所 | 一种刺葡萄VdMYB4基因及其编码蛋白和应用 |
Family Cites Families (66)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4407956A (en) | 1981-03-13 | 1983-10-04 | The Regents Of The University Of California | Cloned cauliflower mosaic virus DNA as a plant vehicle |
| CA1192510A (en) | 1981-05-27 | 1985-08-27 | Lawrence E. Pelcher | Rna plant virus vector or portion thereof, a method of construction thereof, and a method of producing a gene derived product therefrom |
| NL8200523A (nl) | 1982-02-11 | 1983-09-01 | Univ Leiden | Werkwijze voor het in vitro transformeren van planteprotoplasten met plasmide-dna. |
| US4536475A (en) | 1982-10-05 | 1985-08-20 | Phytogen | Plant vector |
| EP0320500B1 (en) | 1983-01-13 | 2004-11-17 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Non-oncogenic ti plasmid vector system and recombinant DNA molecules for the introduction of expressible genes into plant cell genomes |
| NL8300698A (nl) | 1983-02-24 | 1984-09-17 | Univ Leiden | Werkwijze voor het inbouwen van vreemd dna in het genoom van tweezaadlobbige planten; agrobacterium tumefaciens bacterien en werkwijze voor het produceren daarvan; planten en plantecellen met gewijzigde genetische eigenschappen; werkwijze voor het bereiden van chemische en/of farmaceutische produkten. |
| US5504200A (en) | 1983-04-15 | 1996-04-02 | Mycogen Plant Science, Inc. | Plant gene expression |
| EP0160692A1 (en) | 1983-11-03 | 1985-11-13 | DE WET, Johannes Martenis Jacob | Method for the transfer of exogenous genes in plants using pollen as a vector |
| US4992375A (en) | 1983-11-25 | 1991-02-12 | Monsanto Company | Method of regenerating soybeans from cultured soybean cotyledonary nodes |
| US5420034A (en) | 1986-07-31 | 1995-05-30 | Calgene, Inc. | Seed-specific transcriptional regulation |
| US5004863B2 (en) | 1986-12-03 | 2000-10-17 | Agracetus | Genetic engineering of cotton plants and lines |
| US5015580A (en) | 1987-07-29 | 1991-05-14 | Agracetus | Particle-mediated transformation of soybean plants and lines |
| IL84459A (en) | 1986-12-05 | 1993-07-08 | Agracetus | Apparatus and method for the injection of carrier particles carrying genetic material into living cells |
| US5922602A (en) | 1988-02-26 | 1999-07-13 | Biosource Technologies, Inc. | Cytoplasmic inhibition of gene expression |
| US5416011A (en) | 1988-07-22 | 1995-05-16 | Monsanto Company | Method for soybean transformation and regeneration |
| US5086169A (en) | 1989-04-20 | 1992-02-04 | The Research Foundation Of State University Of New York | Isolated pollen-specific promoter of corn |
| JPH04506155A (ja) | 1990-03-16 | 1992-10-29 | カルジーン，インコーポレイティド | 初期種子形成において優先的に発現される新規配列およびそれに関連する方法 |
| JPH07503361A (ja) | 1991-08-01 | 1995-04-13 | バイオソース テクノロジーズ インコーポレイティド | 組み換え植物ウィルス核酸 |
| AU4115693A (en) | 1992-04-24 | 1993-11-29 | Sri International | In vivo homologous sequence targeting in eukaryotic cells |
| ATE398679T1 (de) | 1992-07-07 | 2008-07-15 | Japan Tobacco Inc | Verfahren zur transformation einer monokotyledon pflanze |
| NZ255026A (en) | 1992-07-09 | 1996-04-26 | Pioneer Hi Bred Int | Promoters for pollen-specific genes |
| EP0672752B1 (en) | 1993-09-03 | 2004-05-26 | Japan Tobacco Inc. | Method of transforming monocotyledon by using scutellum of immature embryo |
| US5470353A (en) | 1993-10-20 | 1995-11-28 | Hollister Incorporated | Post-operative thermal blanket |
| GB9324707D0 (en) | 1993-12-02 | 1994-01-19 | Olsen Odd Arne | Promoter |
| US5731179A (en) | 1993-12-08 | 1998-03-24 | Japan Tobacco Inc. | Method for introducing two T-DNAS into plants and vectors therefor |
| DE733059T1 (de) | 1993-12-09 | 1997-08-28 | Univ Jefferson | Verbindungen und verfahren zur ortsspezifischen mutation in eukaryotischen zellen |
| GB9403512D0 (en) | 1994-02-24 | 1994-04-13 | Olsen Odd Arne | Promoter |
| US5470359A (en) | 1994-04-21 | 1995-11-28 | Pioneer Hi-Bred Internation, Inc. | Regulatory element conferring tapetum specificity |
| US5750866A (en) | 1994-09-08 | 1998-05-12 | American Cyanamid Company | AHAS promoter useful for expression of introduced genes in plants |
| AUPN283495A0 (en) | 1995-05-05 | 1995-06-01 | Australian National University, The | Plant promoter activated by fungal infection |
| US5846797A (en) | 1995-10-04 | 1998-12-08 | Calgene, Inc. | Cotton transformation |
| US5977436A (en) | 1997-04-09 | 1999-11-02 | Rhone Poulenc Agrochimie | Oleosin 5' regulatory region for the modification of plant seed lipid composition |
| JP2001518305A (ja) | 1997-09-30 | 2001-10-16 | ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア | 植物種子におけるタンパク質製造 |
| US6555732B1 (en) | 1998-09-14 | 2003-04-29 | Pioneer Hi-Bred International, Inc. | Rac-like genes and methods of use |
| US20120137382A1 (en) * | 1998-09-22 | 2012-05-31 | Mendel Biotechnology, Inc. | Stress tolerance in plants |
| US8030546B2 (en) * | 1998-09-22 | 2011-10-04 | Mendel Biotechnology, Inc. | Biotic and abiotic stress tolerance in plants |
| US6420630B1 (en) | 1998-12-01 | 2002-07-16 | Stine Biotechnology | Methods for tissue culturing and transforming elite inbreds of Zea mays L. |
| WO2000042207A2 (en) | 1999-01-14 | 2000-07-20 | Monsanto Technology Llc | Soybean transformation method |
| BR0110410A (pt) | 2000-04-28 | 2003-07-01 | Basf Ag | Uso do gene ahas 2 mutante x112 de milho e herbicidas de imidazolinona para seleção de mudas transgênicas de monocotiledÈneas de milho, arroz e trigo resistentes aos herbicidas de imidazolinona |
| IL152714A (en) | 2000-05-09 | 2014-03-31 | Xy Llc | High-purity spermatozoa populations carrying chromosome-x and chromosome-y |
| US20090144849A1 (en) * | 2002-02-11 | 2009-06-04 | Lutfiyya Linda L | Nucleic acid molecules and other molecules associated with transcription in plants |
| DE10224889A1 (de) | 2002-06-04 | 2003-12-18 | Metanomics Gmbh & Co Kgaa | Verfahren zur stabilen Expression von Nukleinsäuren in transgenen Pflanzen |
| AU2005252338B2 (en) | 2004-06-07 | 2008-12-18 | Basf Plant Science Gmbh | Improved transformation of soybean |
| BRPI0513291A (pt) | 2004-07-14 | 2008-05-06 | Mendel Biotechnology Inc | polinucleotìdeos de planta para rendimento e qualidade melhorados |
| AU2006260924B2 (en) | 2005-06-23 | 2011-03-03 | Basf Plant Science Gmbh | Improved methods for the production of stably transformed, fertile zea mays plants |
| US8697857B2 (en) | 2007-11-20 | 2014-04-15 | E. I. Du Pont De Nemours And Company | Soybean EF1A2 promoter and its use in constitutive expression of transgenic genes in plants |
| CN102010466B (zh) | 2010-11-10 | 2013-08-21 | 中国农业科学院作物科学研究所 | 植物抗性相关蛋白myb及其编码基因与应用 |
| CN102161697B (zh) * | 2011-02-17 | 2013-03-13 | 南京农业大学 | 菊花抗逆转录因子CmMYB2及其植物表达载体和构建方法及应用 |
| EP2723867A1 (en) | 2011-06-27 | 2014-04-30 | BASF Plant Science Company GmbH | Phacosporacea resistant soybean plants |
| CN102864169A (zh) * | 2011-07-08 | 2013-01-09 | 河北农业大学 | 拟南芥基因myb73在植物抗核盘病方面的应用 |
| WO2013093738A1 (en) | 2011-12-23 | 2013-06-27 | Basf Plant Science Company Gmbh | Genes to enhance disease resistance in crops |
| WO2013092275A2 (en) | 2011-12-23 | 2013-06-27 | Basf Plant Science Company Gmbh | Genes to enhance the defense against pathogens in plants |
| AR092813A1 (es) | 2012-04-05 | 2015-05-06 | Basf Plant Science Co Gmbh | Plantas resistentes a hongos que expresan acd |
| AR094432A1 (es) | 2012-04-05 | 2015-08-05 | Basf Plant Science Co Gmbh | Plantas resistentes a hongos que expresan hidrofobina |
| BR112014025131B1 (pt) | 2012-04-11 | 2022-05-10 | Basf Plant Science Company Gmbh | Métodos de aumento da resistência à ferrugem de soja, construção de vetor recombinante, método de produção de plantas e método de cultivo de plantas |
| CN102676544B (zh) | 2012-05-25 | 2014-02-19 | 复旦大学 | 水稻MYB家族转录因子OsMYB84基因编码序列及其应用 |
| CA2875174A1 (en) | 2012-08-09 | 2014-03-20 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing hcp4 |
| US10329580B2 (en) | 2012-08-09 | 2019-06-25 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing RLK1 |
| BR112015002684B1 (pt) | 2012-08-09 | 2022-05-03 | Basf Plant Science Company Gmbh | Método para aumentar a resistência fúngica, construção de vetor recombinante, método de produção de uma planta transgênica, produto, método de produção de um produto e método de reprodução de uma planta resistente a fungos |
| CA2874985A1 (en) | 2012-08-09 | 2014-02-13 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing hcp5 |
| CN104781273A (zh) | 2012-11-13 | 2015-07-15 | 巴斯夫植物科学有限公司 | 表达casar的真菌抗性植物 |
| WO2014076659A1 (en) | 2012-11-15 | 2014-05-22 | Basf Plant Science Company Gmbh | Method of producing plants having increased resistance to pathogens |
| US10435705B2 (en) | 2013-01-29 | 2019-10-08 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing HCP6 |
| WO2014118018A1 (en) | 2013-01-29 | 2014-08-07 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing ein2 |
| WO2014117988A1 (en) | 2013-01-29 | 2014-08-07 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing hcp7 |
| WO2014135682A1 (en) | 2013-03-08 | 2014-09-12 | Basf Plant Science Company Gmbh | Fungal resistant plants expressing mybtf |
-
2014
- 2014-03-07 WO PCT/EP2014/054461 patent/WO2014135682A1/en not_active Ceased
- 2014-03-07 BR BR112015021857-1A patent/BR112015021857B1/pt active IP Right Grant
- 2014-03-07 CA CA2900005A patent/CA2900005A1/en not_active Abandoned
- 2014-03-07 US US14/772,140 patent/US10465204B2/en active Active
- 2014-03-07 AR ARP140100753A patent/AR096014A1/es unknown
- 2014-03-07 CN CN201480012715.7A patent/CN105008542B/zh active Active
Also Published As
| Publication number | Publication date |
|---|---|
| US20160068856A1 (en) | 2016-03-10 |
| CN105008542A (zh) | 2015-10-28 |
| WO2014135682A1 (en) | 2014-09-12 |
| CN105008542B (zh) | 2020-02-07 |
| AR096014A1 (es) | 2015-12-02 |
| BR112015021857B1 (pt) | 2022-05-10 |
| BR112015021857A2 (https=) | 2017-08-29 |
| US10465204B2 (en) | 2019-11-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US11142774B2 (en) | Fungal resistant plants expressing RLK1 | |
| US10925223B2 (en) | Fungal resistant plants expressing EIN2 | |
| US10941413B2 (en) | Fungal resistant plants expressing CASAR | |
| US11180772B2 (en) | Fungal resistant plants expressing RLK2 | |
| US10462994B2 (en) | Fungal resistant plants expressing HCP7 | |
| US10435705B2 (en) | Fungal resistant plants expressing HCP6 | |
| US10023875B2 (en) | Fungal resistant plants expressing HCP5 | |
| US10465204B2 (en) | Fungal resistant plants expressing MybTF | |
| US9944946B2 (en) | Fungal resistant plants expressing HCP4 | |
| CA2868185A1 (en) | Fungal resistant plants expressing acd | |
| CA2868216A1 (en) | Fungal resistant plants expressing ocp3 | |
| US9957522B2 (en) | Fungal resistant plants expressing CASAR | |
| US20140047577A1 (en) | Fungal Resistant Plants Expressing RLK2 | |
| US20140047576A1 (en) | Fungal Resistant Plants Expressing HCP4 | |
| US20140047578A1 (en) | Fungal Resistant Plants Expressing HCP5 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20180307 |