CA2882276C - Recombinant microorganisms expressing a stereospecific diol dehydratase enzyme - Google Patents

Recombinant microorganisms expressing a stereospecific diol dehydratase enzyme Download PDF

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Publication number
CA2882276C
CA2882276C CA2882276A CA2882276A CA2882276C CA 2882276 C CA2882276 C CA 2882276C CA 2882276 A CA2882276 A CA 2882276A CA 2882276 A CA2882276 A CA 2882276A CA 2882276 C CA2882276 C CA 2882276C
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Prior art keywords
propan
microorganism
diol
clostridium
propane
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CA2882276A
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English (en)
French (fr)
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CA2882276E (en
CA2882276A1 (en
Inventor
Alexander Paul MUELLER
Michael Koepke
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Lanzatech NZ Inc
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Lanzatech New Zealand Ltd
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P41/00Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture
    • C12P41/002Processes using enzymes or microorganisms to separate optical isomers from a racemic mixture by oxidation/reduction reactions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/24Preparation of oxygen-containing organic compounds containing a carbonyl group
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/24Preparation of oxygen-containing organic compounds containing a carbonyl group
    • C12P7/26Ketones
    • C12P7/28Acetone-containing products
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y402/00Carbon-oxygen lyases (4.2)
    • C12Y402/01Hydro-lyases (4.2.1)
    • C12Y402/01028Propanediol dehydratase (4.2.1.28)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2882276A 2012-08-28 2013-08-28 Recombinant microorganisms expressing a stereospecific diol dehydratase enzyme Active CA2882276C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201261694104P 2012-08-28 2012-08-28
US61/694,104 2012-08-28
US201261720224P 2012-10-30 2012-10-30
US61/720,224 2012-10-30
PCT/US2013/057103 WO2014036152A1 (en) 2012-08-28 2013-08-28 Recombinant microorganisms and uses therefor

Publications (3)

Publication Number Publication Date
CA2882276E CA2882276E (en) 2014-03-06
CA2882276A1 CA2882276A1 (en) 2014-03-06
CA2882276C true CA2882276C (en) 2016-11-01

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Family Applications (1)

Application Number Title Priority Date Filing Date
CA2882276A Active CA2882276C (en) 2012-08-28 2013-08-28 Recombinant microorganisms expressing a stereospecific diol dehydratase enzyme

Country Status (9)

Country Link
US (1) US9284564B2 (forum.php)
EP (1) EP2890778B1 (forum.php)
JP (1) JP6302471B2 (forum.php)
KR (1) KR102121888B1 (forum.php)
CN (1) CN105051179B (forum.php)
CA (1) CA2882276C (forum.php)
IN (1) IN2015DN01365A (forum.php)
SG (1) SG11201501254RA (forum.php)
WO (1) WO2014036152A1 (forum.php)

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US10358661B2 (en) 2015-12-28 2019-07-23 Lanzatech New Zealand Limited Microorganism with modified hydrogenase activity
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JPWO2018070141A1 (ja) * 2016-10-13 2019-08-08 積水化学工業株式会社 偏性嫌気性酢酸生成微生物、並びに、組換え微生物
SG11202001716XA (en) 2017-09-08 2020-03-30 Lanzatech Inc Processes and systems for metabolite production using hydrogen rich c1-containing substrates
WO2019126400A1 (en) 2017-12-19 2019-06-27 Lanzatech, Inc. Microorganisms and methods for the biological production of ethylene glycol
BR112020014630A2 (pt) 2018-02-12 2020-12-08 Lanzatech, Inc. Processo para aprimorar a eficiência da conversão de carbono
KR102669528B1 (ko) 2018-04-20 2024-05-29 란자테크, 인크. 간헐적 전기분해 스트림
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BR112021015449A2 (pt) 2019-02-08 2021-10-05 Lanzatech, Inc. Métodos para recuperar produto a partir de um caldo de fermentação e para recuperar produto a partir de uma corrente enriquecida com produto
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KR102346757B1 (ko) * 2019-09-30 2021-12-31 서강대학교산학협력단 1,2-프로필렌글라이콜 생산성이 증강된 메틸영양세균 및 이를 이용한 1,2-프로필렌글라이콜 생산방법
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WO2021188190A1 (en) 2020-03-18 2021-09-23 Lanzatech, Inc. Fermentative production of 2-phenylethanol from gaseous substrates
CN114144513A (zh) 2020-04-29 2022-03-04 朗泽科技有限公司 从气态底物发酵生产β-酮己二酸盐
US11760989B2 (en) 2020-06-06 2023-09-19 Lanzatech, Inc. Microorganism with knock-in at acetolactate decarboxylase gene locus
CA3195088C (en) 2021-02-08 2024-04-23 Fungmin Liew Recombinant microorganisms and their use in the production of 3-hydroxypropionate [3-hp]
JP2024513395A (ja) 2021-04-09 2024-03-25 ランザテク,インコーポレイテッド 供給原料を提供するためのプロセス及び装置
US12241105B2 (en) 2021-07-20 2025-03-04 Lanzatech, Inc. Recombinant microorganisms and uses therefor
TW202307202A (zh) * 2021-08-06 2023-02-16 美商朗澤科技有限公司 用於改良乙二醇之生物產生的微生物及方法
CN117693588A (zh) * 2021-08-06 2024-03-12 朗泽科技有限公司 用于改进乙二醇的生物产生的微生物和方法
US12091648B2 (en) 2021-11-03 2024-09-17 Lanzatech, Inc. System and method for generating bubbles in a vessel
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Also Published As

Publication number Publication date
US9284564B2 (en) 2016-03-15
CN105051179B (zh) 2018-06-15
CN105051179A (zh) 2015-11-11
EP2890778A1 (en) 2015-07-08
SG11201501254RA (en) 2015-03-30
CA2882276E (en) 2014-03-06
WO2014036152A1 (en) 2014-03-06
KR20150045447A (ko) 2015-04-28
US20140065698A1 (en) 2014-03-06
KR102121888B1 (ko) 2020-06-12
CA2882276A1 (en) 2014-03-06
JP6302471B2 (ja) 2018-03-28
EP2890778A4 (en) 2016-03-30
JP2015526105A (ja) 2015-09-10
IN2015DN01365A (forum.php) 2015-07-03
EP2890778B1 (en) 2019-05-01

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