CA2878126C - Fluorescent fusion polypeptide, biosensor comprising said polypeptide and uses thereof - Google Patents

Fluorescent fusion polypeptide, biosensor comprising said polypeptide and uses thereof Download PDF

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Publication number
CA2878126C
CA2878126C CA2878126A CA2878126A CA2878126C CA 2878126 C CA2878126 C CA 2878126C CA 2878126 A CA2878126 A CA 2878126A CA 2878126 A CA2878126 A CA 2878126A CA 2878126 C CA2878126 C CA 2878126C
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CA
Canada
Prior art keywords
seq
peptide
fusion polypeptide
fluorescent
fluorescent fusion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2878126A
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English (en)
French (fr)
Other versions
CA2878126A1 (en
Inventor
Danel KORTAZAR ZABALA
Aida Clarisa SALADO POGONZA
Jorge Gamiz Mata
Meritxell ROURA FERRER
Rosa Mella Lopez
Patricia Villace Lozano
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS SL
Original Assignee
INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS SL
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS SL filed Critical INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS SL
Publication of CA2878126A1 publication Critical patent/CA2878126A1/en
Application granted granted Critical
Publication of CA2878126C publication Critical patent/CA2878126C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5035—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on sub-cellular localization
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/715—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons
    • C07K14/7155—Receptors; Cell surface antigens; Cell surface determinants for cytokines; for lymphokines; for interferons for interleukins [IL]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/03—Fusion polypeptide containing a localisation/targetting motif containing a transmembrane segment
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/60—Fusion polypeptide containing spectroscopic/fluorescent detection, e.g. green fluorescent protein [GFP]
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • Toxicology (AREA)
  • Urology & Nephrology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2878126A 2012-07-06 2013-07-08 Fluorescent fusion polypeptide, biosensor comprising said polypeptide and uses thereof Active CA2878126C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12382272.8 2012-07-06
EP12382272 2012-07-06
PCT/EP2013/064400 WO2014006225A1 (en) 2012-07-06 2013-07-08 Fluorescent fusion polypeptide, biosensor comprising said polypeptide and uses thereof

Publications (2)

Publication Number Publication Date
CA2878126A1 CA2878126A1 (en) 2014-01-09
CA2878126C true CA2878126C (en) 2022-05-10

Family

ID=48803514

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2878126A Active CA2878126C (en) 2012-07-06 2013-07-08 Fluorescent fusion polypeptide, biosensor comprising said polypeptide and uses thereof

Country Status (9)

Country Link
US (3) US9784728B2 (https=)
EP (1) EP2870475B1 (https=)
JP (1) JP6393260B2 (https=)
CN (1) CN104781667B (https=)
CA (1) CA2878126C (https=)
DK (1) DK2870475T3 (https=)
ES (1) ES2645431T3 (https=)
IN (1) IN2014MN02585A (https=)
WO (1) WO2014006225A1 (https=)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IN2014MN02585A (https=) 2012-07-06 2015-07-24 Innovative Technologies In Biolog Systems S L
US9784726B2 (en) 2013-01-08 2017-10-10 Atrogi Ab Screening method, a kit, a method of treatment and a compound for use in a method of treatment
EP3577456B9 (en) * 2017-02-06 2023-10-04 Innovative Technologies In Biological Systems SL Fluorescent fusion polypeptide capable of detecting phosphorylation of cellular membrane receptors by grks (g-protein receptor kinases) and/or receptor tyrosine kinases (rtks)

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH089640B2 (ja) * 1994-05-19 1996-01-31 佑 本庶 ヒト・インターロイキン−２受容体
US6197928B1 (en) 1997-03-14 2001-03-06 The Regents Of The University Of California Fluorescent protein sensors for detection of analytes
US7192932B1 (en) * 1997-04-29 2007-03-20 Lauras As Use of immunomodulating agents
CA2331917A1 (en) * 1998-05-20 1999-11-25 Chugai Seiyaku Kabushiki Kaisha Novel method for gene cloning
EP1131081A4 (en) * 1998-11-12 2006-09-06 Beth Israel Hospital STIMULATION OF ANGIOGENESIS BY THE SIGNALING PATHWAY OF THE SYNDECAN-4 CYTOPLASMIC DOMAIN
US7060793B2 (en) * 1999-05-21 2006-06-13 The Regents Of The University Of California Circularly permuted fluorescent protein indicators
US20070037165A1 (en) * 2000-09-08 2007-02-15 Applera Corporation Polymorphisms in known genes associated with human disease, methods of detection and uses thereof
US7125660B2 (en) * 2000-09-13 2006-10-24 Archemix Corp. Nucleic acid sensor molecules and methods of using same
CA2453528C (en) * 2001-08-01 2011-07-26 Cellomics, Inc. Novel fusion proteins and assays for molecular binding
CA2497254A1 (en) * 2002-09-06 2004-03-18 Forschungsverbund Berlin E. V. Akap18 delta, a novel splicing variant of a protein kinase a anchor protein and the use of the same
JP4214206B2 (ja) * 2002-12-10 2009-01-28 独立行政法人理化学研究所 Ｆｒｅｔを利用した蛍光指示薬
US7488583B2 (en) * 2003-09-25 2009-02-10 Odyssey Thera, Inc. Fragment complementation assays for G-protein-coupled receptors and their signaling pathways
GB0411586D0 (en) * 2004-05-24 2004-06-23 Univ The Glasgow Fluorescent protein
US20090131270A1 (en) * 2004-08-02 2009-05-21 Cellumen, Inc.A Corporation Methods for the detection of molecular interactions within cells
EP1997885A1 (en) * 2007-05-29 2008-12-03 AXXAM S.p.A. Fluorescent proteins and methods of use thereof
US20110154515A1 (en) * 2007-08-24 2011-06-23 Oliver Griesbeck Genetically encoded calcium sensors comprising the c-terminal lobe of troponin c and a fluorescence tag
JP2009153399A (ja) * 2007-12-25 2009-07-16 National Institute Of Advanced Industrial & Technology 一分子型リアルタイム生物発光イメージングプローブ
CN102348807B (zh) * 2009-01-12 2015-04-15 苏特罗生物制药公司 利用体外蛋白合成体系将非天然氨基酸引入蛋白的单装载体系
IN2012DN01331A (https=) * 2009-08-31 2015-06-05 Ibc Pharmaceuticals Inc
IN2014MN02585A (https=) 2012-07-06 2015-07-24 Innovative Technologies In Biolog Systems S L

Also Published As

Publication number Publication date
DK2870475T3 (en) 2017-09-18
CN104781667B (zh) 2017-06-13
EP2870475B1 (en) 2017-06-07
JP6393260B2 (ja) 2018-09-19
CA2878126A1 (en) 2014-01-09
US10883981B2 (en) 2021-01-05
JP2015522582A (ja) 2015-08-06
US10877025B2 (en) 2020-12-29
US20180003697A1 (en) 2018-01-04
US9784728B2 (en) 2017-10-10
US20180003698A1 (en) 2018-01-04
EP2870475A1 (en) 2015-05-13
WO2014006225A1 (en) 2014-01-09
IN2014MN02585A (https=) 2015-07-24
US20150185205A1 (en) 2015-07-02
ES2645431T3 (es) 2017-12-05
CN104781667A (zh) 2015-07-15

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