CA2872056A1 - Spider silk fusion protein structures without repetitive fragment for binding to an organic target - Google Patents

Spider silk fusion protein structures without repetitive fragment for binding to an organic target Download PDF

Info

Publication number
CA2872056A1
CA2872056A1 CA2872056A CA2872056A CA2872056A1 CA 2872056 A1 CA2872056 A1 CA 2872056A1 CA 2872056 A CA2872056 A CA 2872056A CA 2872056 A CA2872056 A CA 2872056A CA 2872056 A1 CA2872056 A1 CA 2872056A1
Authority
CA
Canada
Prior art keywords
protein
fusion protein
moiety
organic target
recombinant fusion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2872056A
Other languages
English (en)
French (fr)
Inventor
My Hedhammar
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Spiber Technologies AB
Original Assignee
Spiber Technologies AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Spiber Technologies AB filed Critical Spiber Technologies AB
Publication of CA2872056A1 publication Critical patent/CA2872056A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/315—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Streptococcus (G), e.g. Enterococci
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/12—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from bacteria
    • C07K16/1267—Gram-positive bacteria
    • C07K16/1271—Micrococcaceae (F); Staphylococcaceae (F), e.g. Staphylococcus (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0081—Purging biological preparations of unwanted cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/30—Non-immunoglobulin-derived peptide or protein having an immunoglobulin constant or Fc region, or a fragment thereof, attached thereto
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/735—Fusion polypeptide containing domain for protein-protein interaction containing a domain for self-assembly, e.g. a viral coat protein (includes phage display)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T428/00—Stock material or miscellaneous articles
    • Y10T428/29—Coated or structually defined flake, particle, cell, strand, strand portion, rod, filament, macroscopic fiber or mass thereof
    • Y10T428/2982—Particulate matter [e.g., sphere, flake, etc.]
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T442/00—Fabric [woven, knitted, or nonwoven textile or cloth, etc.]
    • Y10T442/10—Scrim [e.g., open net or mesh, gauze, loose or open weave or knit, etc.]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Immunology (AREA)
  • Insects & Arthropods (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2872056A 2012-05-02 2013-05-02 Spider silk fusion protein structures without repetitive fragment for binding to an organic target Abandoned CA2872056A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12166393 2012-05-02
EP12166393.4 2012-05-02
PCT/EP2013/059146 WO2013164405A1 (en) 2012-05-02 2013-05-02 Spider silk fusion protein structures without repetitive fragment for binding to an organic target

Publications (1)

Publication Number Publication Date
CA2872056A1 true CA2872056A1 (en) 2013-11-07

Family

ID=48289167

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2872056A Abandoned CA2872056A1 (en) 2012-05-02 2013-05-02 Spider silk fusion protein structures without repetitive fragment for binding to an organic target

Country Status (7)

Country Link
US (1) US9708376B2 (enExample)
EP (1) EP2844360B1 (enExample)
JP (1) JP6412866B2 (enExample)
CN (1) CN104394953B (enExample)
AU (1) AU2013255815B2 (enExample)
CA (1) CA2872056A1 (enExample)
WO (1) WO2013164405A1 (enExample)

Families Citing this family (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101657298B1 (ko) * 2016-02-03 2016-09-13 (주)넥스젠바이오텍 세포 증식 효과가 증가한 인간 상피세포재생인자 융합단백질 및 이를 유효성분으로 함유하는 피부 주름 및 탄력 개선용 화장료 조성물
EP4375403A3 (en) 2016-02-11 2024-08-28 Seevix Material Sciences Ltd. Composite materials comprising synthetic dragline spider silk
EP3263593A1 (en) * 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
WO2018229341A1 (en) * 2017-06-13 2018-12-20 Aalto University Foundation Sr. Method for producing a condensed adhesive phase of silk fusion proteins
US20230201413A1 (en) * 2019-03-11 2023-06-29 Seevix Material Sciences Ltd. Compositions comprising dragline spider silk
JP7709383B2 (ja) * 2019-04-02 2025-07-16 ボンドウェル テクノロジーズ エルピー 抗体の精製を促進するための機能化ｕｂｘタンパク質物質
US12668828B2 (en) 2019-07-04 2026-06-30 Seevix Material Sciences Ltd. Prokaryotic expression system and methods of using the same
KR20230005173A (ko) 2020-04-23 2023-01-09 시비스 머테리얼 사이언스 리미티드 드래그라인 스파이더 실크를 포함하는 화장료 조성물

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994029450A2 (en) * 1993-06-15 1994-12-22 E.I. Du Pont De Nemours And Company Novel, recombinantly produced spider silk analogs
EP0704532B1 (en) 1994-06-10 2002-09-11 United States Surgical Corporation Recombinant chimeric proteins and methods of use thereof
JP2000125872A (ja) 1998-09-07 2000-05-09 Terumo Corp 三量体キメラタンパク質およびキメラタンパク質を含有するコラーゲンマトリックス
DE19932688B4 (de) 1999-07-13 2009-10-08 Scil Proteins Gmbh Design von Beta-Faltblatt-Proteinen des gamma-II-kristallins antikörperähnlichen
GB9917027D0 (en) 1999-07-20 1999-09-22 Affibody Technology Sweeden Ab In vitro selection and optional identification of polypeptides using solid support carriers
US20050261479A1 (en) 2004-04-29 2005-11-24 Christian Hoffmann Method for purifying and recovering silk proteins using magnetic affinity separation
WO2006076711A2 (en) 2005-01-14 2006-07-20 Trustees Of Tufts College Fibrous protein fusions and use thereof in the formation of advanced organic/inorganic composite materials
RU2444527C2 (ru) * 2005-12-30 2012-03-10 Спибер Текнолоджиз Аб Рекомбинантный спидроиновый белок главной ампулярной железы, способный к полимеризации, рекомбинантный слитый белок, способный к управляемой полимеризации, полимер спидроинового белка главной ампулярной железы, композиция для управляемой полимеризации, рекомбинантная молекула нуклеиновой кислоты (варианты), способ получения растворимого слитого белка, способ получения полимера спидроинового белка главной ампулярной железы и способ культивирования эукариотических клеток
EP1852470A1 (en) 2006-05-03 2007-11-07 Technische Universität München Multilayer Silk Protein Films
CA2753287A1 (en) 2009-02-24 2010-09-02 Glaxo Group Limited Antigen-binding constructs
CA2759465C (en) * 2009-04-22 2017-12-12 Spiber Technologies Ab Method of producing polymers of spider silk proteins
EP3168229B1 (en) * 2010-03-18 2019-06-19 Spiber Technologies AB Production of proteins and polypeptides
CN102946914B (zh) * 2010-04-12 2015-06-03 思百博技术股份公司 方法及组合物

Also Published As

Publication number Publication date
US9708376B2 (en) 2017-07-18
JP2015515969A (ja) 2015-06-04
AU2013255815B2 (en) 2018-03-29
WO2013164405A1 (en) 2013-11-07
CN104394953B (zh) 2018-01-23
JP6412866B2 (ja) 2018-10-24
AU2013255815A1 (en) 2014-11-13
CN104394953A (zh) 2015-03-04
US20150087046A1 (en) 2015-03-26
EP2844360B1 (en) 2018-12-19
EP2844360A1 (en) 2015-03-11

Similar Documents

Publication Publication Date Title
US9309299B2 (en) Spider silk fusion protein structures for binding to an organic target
US9708376B2 (en) Spider silk fusion protein structures without repetitive fragment for binding to an organic target
US9856308B2 (en) Spider silk fusion protein structures incorporating immunoglobulin fragments as affinity ligands
CA2990523C (en) Cyclic rgd cell-binding motif and uses thereof
CN102395601A (zh) 生产蜘蛛丝蛋白聚合物的方法
Ramezaniaghdam et al. Recombinant production of spider silk protein in Physcomitrella photobioreactors
CN117241866A (zh) Aav2亲和剂

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20180430

FZDE Dead

Effective date: 20210125