CA2865948A1 - System and method for time-resolved fluorescence imaging and pulse shaping - Google Patents

System and method for time-resolved fluorescence imaging and pulse shaping Download PDF

Info

Publication number
CA2865948A1
CA2865948A1 CA2865948A CA2865948A CA2865948A1 CA 2865948 A1 CA2865948 A1 CA 2865948A1 CA 2865948 A CA2865948 A CA 2865948A CA 2865948 A CA2865948 A CA 2865948A CA 2865948 A1 CA2865948 A1 CA 2865948A1
Authority
CA
Canada
Prior art keywords
image
fluorophore
recited
medium
pulse
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2865948A
Other languages
English (en)
French (fr)
Inventor
Warren S. Grundfest
Oscar M. Stafsudd
Pei-Chi JIANG
Asael PAPOUR
Zachary Deis TAYLOR
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of California, San Diego
Original Assignee
University of California, San Diego
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of California, San Diego filed Critical University of California, San Diego
Publication of CA2865948A1 publication Critical patent/CA2865948A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6447—Fluorescence; Phosphorescence by visual observation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G01N21/6458—Fluorescence microscopy
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6486—Measuring fluorescence of biological material, e.g. DNA, RNA, cells
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/16—Microscopes adapted for ultraviolet illumination ; Fluorescence microscopes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G01N2021/6413—Distinction short and delayed fluorescence or phosphorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/062—LED's
    • G01N2201/0621—Supply

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
CA2865948A 2012-03-02 2013-03-01 System and method for time-resolved fluorescence imaging and pulse shaping Abandoned CA2865948A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261605844P 2012-03-02 2012-03-02
US61/605,844 2012-03-02
PCT/US2013/028758 WO2013131062A1 (en) 2012-03-02 2013-03-01 System and method for time-resolved fluorescence imaging and pulse shaping

Publications (1)

Publication Number Publication Date
CA2865948A1 true CA2865948A1 (en) 2013-09-06

Family

ID=49083367

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2865948A Abandoned CA2865948A1 (en) 2012-03-02 2013-03-01 System and method for time-resolved fluorescence imaging and pulse shaping

Country Status (6)

Country Link
US (1) US10041883B2 (enExample)
EP (1) EP2820398B1 (enExample)
JP (1) JP6389763B2 (enExample)
AU (1) AU2013225655A1 (enExample)
CA (1) CA2865948A1 (enExample)
WO (1) WO2013131062A1 (enExample)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6389763B2 (ja) 2012-03-02 2018-09-12 ザ リージェンツ オブ ザ ユニヴァーシティー オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ 時間分解蛍光画像化およびパルス成形のためのシステムおよび方法
WO2014168734A1 (en) 2013-03-15 2014-10-16 Cedars-Sinai Medical Center Time-resolved laser-induced fluorescence spectroscopy systems and uses thereof
FR3013452B1 (fr) * 2013-11-21 2016-02-05 Centre Nat Rech Scient Procede de detection d'une espece fluorescente reversiblement photoconvertible
JP6542044B2 (ja) 2015-06-30 2019-07-10 オリンパス株式会社 顕微鏡システム
CN115561211A (zh) 2016-04-01 2023-01-03 黑光外科公司 用于时间分辨荧光光谱法的系统、装置和方法
EP3494384A4 (en) * 2016-08-03 2020-03-11 Royal Melbourne Institute Of Technology METHOD FOR DISTINATING BETWEEN SEVERAL FLUORESCENT SPECIES IN A SAMPLE
JP7079244B2 (ja) 2016-10-04 2022-06-01 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア ターゲット識別と境界検出のための多周波数高調波アコーストグラフィ
KR101859172B1 (ko) * 2016-12-22 2018-05-16 (주) 인텍플러스 아날로그 평균지연 방법에서 수집한 신호로부터 가상의 형광분포 모델을 통해 최소자승오차를 계산하여 2개 이상의 형광수명 성분을 구하는 형광수명 측정장치 및 그 측정방법
WO2019089998A1 (en) * 2017-11-01 2019-05-09 The Regents Of The University Of California Imaging method and system for intraoperative surgical margin assessment
KR102126694B1 (ko) 2018-06-01 2020-06-25 주식회사 수젠텍 시간분해 형광신호 분석장치
JP2020071152A (ja) * 2018-10-31 2020-05-07 ソニー株式会社 免疫染色方法、免疫染色システム、および免疫染色キット
CN113508327B (zh) 2019-02-01 2024-08-30 统雷有限公司 高动态范围成像
KR20220098147A (ko) 2019-10-17 2022-07-11 씨2센스, 인크. 감지 및/또는 인증을 위한 발광 이미징
US20210116377A1 (en) 2019-10-17 2021-04-22 C2Sense, Inc. White light emissive species and related methods
WO2021211668A1 (en) 2020-04-14 2021-10-21 The Regents Of The University Of California Method and system for selective spectral illumination for optical image guided surgery
US12660997B2 (en) 2020-04-29 2026-06-23 The Regents Of The University Of California System and method for dynamic optical contrast imaging
US12209964B2 (en) * 2020-10-15 2025-01-28 Visera Technologies Company Limited Bio-chip, bio-detection system and bio-detection method
US20240338792A1 (en) * 2023-04-06 2024-10-10 Thermo Electron Scientific Instruments Llc Adaptive subtraction for c-sim microscopy
US20260063551A1 (en) * 2024-09-03 2026-03-05 James Jiang Optical Imaging System

Family Cites Families (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4791310A (en) * 1986-10-02 1988-12-13 Syracuse University Fluorescence microscopy
US4785495A (en) 1987-08-17 1988-11-22 Dellis Edward A Moldable hand grips
JP2977746B2 (ja) * 1995-08-23 1999-11-15 旭テクノグラス株式会社 蛍光ガラス線量計測定方法及びその測定装置
US6272376B1 (en) * 1999-01-22 2001-08-07 Cedars-Sinai Medical Center Time-resolved, laser-induced fluorescence for the characterization of organic material
US6690247B2 (en) * 1999-02-05 2004-02-10 Northrop Grumman Corporation Nonlinear transmission line waveform generator having an input voltage matched to the C/V characteristic of the transmission line
US7890157B2 (en) 2003-08-19 2011-02-15 Cedars-Sinai Medical Center Method for fluorescence lifetime imaging microscopy and spectroscopy
JP2005269051A (ja) * 2004-03-17 2005-09-29 Sysmex Corp 発光ダイオードの駆動回路
US7812324B2 (en) * 2004-10-18 2010-10-12 Macquarie University Fluorescence detection
US7193486B2 (en) * 2005-01-19 2007-03-20 Northrop Grumman Corporation Tunable, maximum power output, frequency harmonic comb generator
JP2006226848A (ja) * 2005-02-17 2006-08-31 Olympus Corp 蛍光撮像装置
EP2703871A3 (en) * 2005-05-25 2014-09-03 Massachusetts Institute Of Technology Multifocal scanning microscopy systems and methods
US7648239B2 (en) 2007-05-01 2010-01-19 Richard Spaide Autofluorescence photography using a fundus camera
JP4538033B2 (ja) * 2007-09-10 2010-09-08 株式会社沖データ 駆動回路、ｌｅｄヘッドおよび画像形成装置
JP2010071982A (ja) * 2008-08-20 2010-04-02 Japan Atomic Energy Agency レーザー誘起蛍光強度の減衰率比較によるアスベスト識別法
GB0905690D0 (en) 2009-04-01 2009-05-20 Univ Cardiff Fluorescence detection schemes
JP2011185842A (ja) * 2010-03-10 2011-09-22 Fujifilm Corp 光誘起自家蛍光の時間分解測定による生物試料の低酸素領域分析方法とその装置
JP5437864B2 (ja) * 2010-03-10 2014-03-12 富士フイルム株式会社 ｐＨ測定装置の作動方法、検出装置の作動方法、及び生体物質分析装置の作動方法、並びに各装置
JP5626760B2 (ja) * 2010-03-31 2014-11-19 ソニー株式会社 蛍光像取得方法、蛍光像取得プログラム及び蛍光像取得装置
GB201007055D0 (en) * 2010-04-28 2010-06-09 Vib Vzw Method and apparatus for the imaging of a labelled sample
BR112013009910A2 (pt) 2010-10-25 2020-09-01 Steven Verdooner aparelho e método para detectar amilóide em uma retina num diagnóstico, avanço e prognóstico da doença de alzheimer, traumatismo craniano, degeneração macular e uma pluraridade de disfunções neurodegenarativas, e doenças oculares
US10222335B2 (en) 2010-10-27 2019-03-05 The Regents Of The University Of California Phasor method to fluorescence lifetime microscopy to discriminate metabolic state of cells in living tissue
JP6389763B2 (ja) 2012-03-02 2018-09-12 ザ リージェンツ オブ ザ ユニヴァーシティー オブ カリフォルニアＴｈｅ Ｒｅｇｅｎｔｓ Ｏｆ Ｔｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃａｌｉｆｏｒｎｉａ 時間分解蛍光画像化およびパルス成形のためのシステムおよび方法

Also Published As

Publication number Publication date
EP2820398A4 (en) 2016-01-20
JP6389763B2 (ja) 2018-09-12
JP2015508905A (ja) 2015-03-23
WO2013131062A1 (en) 2013-09-06
US20150053871A1 (en) 2015-02-26
US10041883B2 (en) 2018-08-07
EP2820398A1 (en) 2015-01-07
EP2820398B1 (en) 2018-10-24
AU2013225655A1 (en) 2014-09-18

Similar Documents

Publication Publication Date Title
US10041883B2 (en) System and method for time-resolved fluorescence imaging and pulse shaping
JP5265408B2 (ja) 相関分光分析方法及び相関分光分析装置
CN108463714B (zh) 用于测量电子激发态的平均寿命的发射寿命测量方法和设备
JP5623278B2 (ja) 顕微鏡および顕微鏡の操作方法
JP5935147B2 (ja) 顕微鏡システム、顕微鏡法、及び記憶媒体
EP2150805B1 (en) Methods of fluorescence imaging, computer program, computerprogram product and fluorescence imaging system
US9784678B2 (en) Method for improving fluorescence image contrast
CN109477784A (zh) 具有增强图像分辨率的荧光成像流式细胞术
JPS6332356A (ja) 蛍光信号の解析と画像表示のための装置
WO2017088656A1 (zh) 一种荧光寿命测量方法及装置
KR101847334B1 (ko) 형광 이미지 획득 장치 및 방법
JP2015508905A5 (enExample)
WO2006135769A1 (en) Fluorescence-lifetime-based tomography
US9885705B2 (en) Cell analysis using dynamic biophysical methods
JP4474582B2 (ja) 蛍光の検出方法
KR20170062812A (ko) 에너지 전이에 따른 형광 수명을 측정하는 방법
WO2015037055A1 (ja) 蛍光画像取得装置
JP2012522980A (ja) 蛍光寿命イメージング
JP2009058405A (ja) 光分析装置
Valeur Pulse and phase fluorometries: an objective comparison
Wohlschläger et al. An approach of identifying polymers with fluorescence lifetime imaging
WO2025029181A1 (en) A method of performing a fluorescence measurement, a controller and an apparatus
Zhao et al. mb-FLIM: Model-based fluorescence lifetime imaging
JP2008180637A (ja) 光信号解析方法および光信号解析装置
Liu et al. Lifetime computing algorithms based on exponential pattern retrieve and polynomial fitting in fluorescence lifetime imaging microscopy

Legal Events

Date Code Title Description
FZDE Dead

Effective date: 20180301