CA2832367A1 - Methods of purifying viruses using gel permeation chromatography - Google Patents
Methods of purifying viruses using gel permeation chromatography Download PDFInfo
- Publication number
- CA2832367A1 CA2832367A1 CA2832367A CA2832367A CA2832367A1 CA 2832367 A1 CA2832367 A1 CA 2832367A1 CA 2832367 A CA2832367 A CA 2832367A CA 2832367 A CA2832367 A CA 2832367A CA 2832367 A1 CA2832367 A1 CA 2832367A1
- Authority
- CA
- Canada
- Prior art keywords
- virus
- elution buffer
- gel permeation
- permeation chromatography
- buffered saline
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/34—Size-selective separation, e.g. size-exclusion chromatography; Gel filtration; Permeation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/42—Selective adsorption, e.g. chromatography characterised by the development mode, e.g. by displacement or by elution
- B01D15/424—Elution mode
- B01D15/426—Specific type of solvent
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/12011—Reoviridae
- C12N2720/12051—Methods of production or purification of viral material
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Virology (AREA)
- Immunology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Medicinal Preparation (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161480561P | 2011-04-29 | 2011-04-29 | |
| US61/480,561 | 2011-04-29 | ||
| PCT/CA2012/000406 WO2012145837A1 (en) | 2011-04-29 | 2012-04-27 | Methods of purifying viruses using gel permeation chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2832367A1 true CA2832367A1 (en) | 2012-11-01 |
Family
ID=47067091
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2832367A Abandoned CA2832367A1 (en) | 2011-04-29 | 2012-04-27 | Methods of purifying viruses using gel permeation chromatography |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US20120273424A1 (https=) |
| EP (1) | EP2702147B1 (https=) |
| JP (1) | JP6029651B2 (https=) |
| KR (2) | KR20140024406A (https=) |
| CN (1) | CN103492557B (https=) |
| AU (1) | AU2012248082B2 (https=) |
| CA (1) | CA2832367A1 (https=) |
| DK (1) | DK2702147T3 (https=) |
| EA (1) | EA201391605A1 (https=) |
| ES (1) | ES2822199T3 (https=) |
| IL (1) | IL228491A (https=) |
| MX (1) | MX337348B (https=) |
| SG (1) | SG194449A1 (https=) |
| TW (1) | TW201307561A (https=) |
| WO (1) | WO2012145837A1 (https=) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025181504A1 (en) * | 2024-02-29 | 2025-09-04 | Pécsi Tudományegyetem | Purification of infective virus |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU2011336413B2 (en) * | 2010-12-02 | 2015-01-22 | Oncolytics Biotech Inc. | Liquid viral formulations |
| MX349294B (es) | 2010-12-02 | 2017-07-21 | Oncolytics Biotech Inc | Formulaciones virales liofilizadas. |
| BR112015031541A2 (pt) | 2013-06-17 | 2017-07-25 | De Staat Der Nederlanden Vert Door De Mini Van Vws Miniie Van Volksgezondheid Welzijn En Sport | métodos para o impedimento da agregação de componentes virais |
| US11999975B2 (en) | 2018-07-04 | 2024-06-04 | Probiogen Ag | Method for purifying an enveloped virus |
| CN111812313B (zh) * | 2020-06-22 | 2021-10-08 | 北京生物制品研究所有限责任公司 | 铝佐剂吸附型新型冠状病毒灭活疫苗中抗原的解离方法 |
| CN114544815B (zh) * | 2022-03-01 | 2023-10-27 | 中牧实业股份有限公司 | 一种山羊痘病毒的定量检测方法 |
| CN116718524B (zh) * | 2023-06-26 | 2025-11-07 | 西安交通大学 | 一种基于细胞膜色谱技术的病毒颗粒检测方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5096885A (en) * | 1988-04-15 | 1992-03-17 | Genentech, Inc. | Human growth hormone formulation |
| DE69029765T2 (de) * | 1989-07-24 | 1997-05-15 | Bayer Ag | Stabilisierung von hochgereinigten Proteinen |
| JP3863559B2 (ja) * | 1994-05-16 | 2006-12-27 | メルク エンド カンパニー インコーポレーテッド | 乳頭腫ウィルスワクチン |
| KR100912362B1 (ko) * | 1998-02-17 | 2009-08-19 | 쉐링 코포레이션 | 바이러스 제제의 정제방법 |
| DK2253701T3 (da) | 2001-03-16 | 2014-10-20 | Oncolytics Biotech Inc | Fremgangsmåde til ekstraktion af en virus fra en cellekultur |
| CA2482512C (en) * | 2002-04-30 | 2011-09-20 | Oncolytics Biotech Inc. | Improved viral purification methods |
| US7901921B2 (en) * | 2004-10-22 | 2011-03-08 | Oncolytics Biotech Inc. | Viral purification methods |
| EP1878791A1 (en) * | 2006-07-11 | 2008-01-16 | Bia Separations D.O.O. | Method for influenza virus purification |
| EP2055311B1 (en) * | 2006-08-07 | 2017-06-21 | The Chemo-Sero-Therapeutic Research Institute | Process for preparing live smallpox vaccine |
| JP5577103B2 (ja) * | 2007-03-12 | 2014-08-20 | オンコリティクス バイオテク，インコーポレーテッド | 改変配列を有するレオウイルス |
| NZ585500A (en) * | 2007-11-12 | 2012-06-29 | Theraclone Sciences Inc | Antibodies which bind to an epitope in the extracellular domain of the matrix 2 ectodomain polypeptide of an influenza virus |
| US8227577B2 (en) * | 2007-12-21 | 2012-07-24 | Hoffman-La Roche Inc. | Bivalent, bispecific antibodies |
| MX349294B (es) * | 2010-12-02 | 2017-07-21 | Oncolytics Biotech Inc | Formulaciones virales liofilizadas. |
| AU2011336413B2 (en) * | 2010-12-02 | 2015-01-22 | Oncolytics Biotech Inc. | Liquid viral formulations |
-
2012
- 2012-04-27 JP JP2014506699A patent/JP6029651B2/ja active Active
- 2012-04-27 EP EP12776970.1A patent/EP2702147B1/en active Active
- 2012-04-27 KR KR1020137031292A patent/KR20140024406A/ko not_active Ceased
- 2012-04-27 WO PCT/CA2012/000406 patent/WO2012145837A1/en not_active Ceased
- 2012-04-27 EA EA201391605A patent/EA201391605A1/ru unknown
- 2012-04-27 AU AU2012248082A patent/AU2012248082B2/en active Active
- 2012-04-27 SG SG2013075569A patent/SG194449A1/en unknown
- 2012-04-27 TW TW101115116A patent/TW201307561A/zh unknown
- 2012-04-27 CA CA2832367A patent/CA2832367A1/en not_active Abandoned
- 2012-04-27 ES ES12776970T patent/ES2822199T3/es active Active
- 2012-04-27 MX MX2013012627A patent/MX337348B/es active IP Right Grant
- 2012-04-27 KR KR1020197006419A patent/KR102151890B1/ko active Active
- 2012-04-27 US US13/457,642 patent/US20120273424A1/en not_active Abandoned
- 2012-04-27 DK DK12776970.1T patent/DK2702147T3/da active
- 2012-04-27 CN CN201280020376.8A patent/CN103492557B/zh active Active
-
2013
- 2013-09-17 IL IL228491A patent/IL228491A/en active IP Right Grant
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2025181504A1 (en) * | 2024-02-29 | 2025-09-04 | Pécsi Tudományegyetem | Purification of infective virus |
Also Published As
| Publication number | Publication date |
|---|---|
| KR20190026059A (ko) | 2019-03-12 |
| MX2013012627A (es) | 2014-05-13 |
| JP2014516515A (ja) | 2014-07-17 |
| KR102151890B1 (ko) | 2020-09-03 |
| IL228491A (en) | 2017-05-29 |
| EP2702147B1 (en) | 2020-07-15 |
| SG194449A1 (en) | 2013-12-30 |
| AU2012248082A1 (en) | 2013-05-09 |
| IL228491A0 (en) | 2013-12-31 |
| AU2012248082B2 (en) | 2015-08-20 |
| WO2012145837A1 (en) | 2012-11-01 |
| CN103492557A (zh) | 2014-01-01 |
| ES2822199T3 (es) | 2021-04-29 |
| MX337348B (es) | 2016-02-29 |
| KR20140024406A (ko) | 2014-02-28 |
| US20120273424A1 (en) | 2012-11-01 |
| EA201391605A1 (ru) | 2014-02-28 |
| EP2702147A1 (en) | 2014-03-05 |
| EP2702147A4 (en) | 2014-11-05 |
| CN103492557B (zh) | 2015-07-29 |
| TW201307561A (zh) | 2013-02-16 |
| DK2702147T3 (da) | 2020-09-28 |
| JP6029651B2 (ja) | 2016-11-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20170424 |
|
| FZDE | Discontinued |
Effective date: 20201007 |