CA2829918A1 - Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars - Google Patents
Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars Download PDFInfo
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- CA2829918A1 CA2829918A1 CA2829918A CA2829918A CA2829918A1 CA 2829918 A1 CA2829918 A1 CA 2829918A1 CA 2829918 A CA2829918 A CA 2829918A CA 2829918 A CA2829918 A CA 2829918A CA 2829918 A1 CA2829918 A1 CA 2829918A1
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- glucosidase
- amino acid
- polypeptide
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- 235000017550 sodium carbonate Nutrition 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 239000001488 sodium phosphate Substances 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 238000010361 transduction Methods 0.000 description 1
- 230000026683 transduction Effects 0.000 description 1
- 238000011426 transformation method Methods 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 1
- 238000000108 ultra-filtration Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 125000000969 xylosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)CO1)* 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
-
- D—TEXTILES; PAPER
- D06—TREATMENT OF TEXTILES OR THE LIKE; LAUNDERING; FLEXIBLE MATERIALS NOT OTHERWISE PROVIDED FOR
- D06M—TREATMENT, NOT PROVIDED FOR ELSEWHERE IN CLASS D06, OF FIBRES, THREADS, YARNS, FABRICS, FEATHERS OR FIBROUS GOODS MADE FROM SUCH MATERIALS
- D06M16/00—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic
- D06M16/003—Biochemical treatment of fibres, threads, yarns, fabrics, or fibrous goods made from such materials, e.g. enzymatic with enzymes or microorganisms
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Textile Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161453918P | 2011-03-17 | 2011-03-17 | |
| US61/453,918 | 2011-03-17 | ||
| PCT/US2012/029498 WO2012125951A1 (en) | 2011-03-17 | 2012-03-16 | Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2829918A1 true CA2829918A1 (en) | 2012-09-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2829918A Abandoned CA2829918A1 (en) | 2011-03-17 | 2012-03-16 | Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars |
Country Status (13)
| Country | Link |
|---|---|
| US (2) | US20140073017A1 (enExample) |
| EP (1) | EP2686427A1 (enExample) |
| JP (1) | JP6148183B2 (enExample) |
| KR (1) | KR20140023313A (enExample) |
| CN (2) | CN109371002A (enExample) |
| AU (1) | AU2012228968B2 (enExample) |
| BR (1) | BR112013023715A2 (enExample) |
| CA (1) | CA2829918A1 (enExample) |
| MX (1) | MX2013010509A (enExample) |
| RU (1) | RU2013146341A (enExample) |
| SG (1) | SG192097A1 (enExample) |
| WO (1) | WO2012125951A1 (enExample) |
| ZA (1) | ZA201305532B (enExample) |
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| EP2480660B1 (en) | 2009-09-23 | 2020-08-05 | Danisco US Inc. | Novel glycosyl hydrolase enzymes and uses thereof |
| CN107287250A (zh) | 2009-12-23 | 2017-10-24 | 丹尼斯科美国公司 | 提高同步糖化发酵反应效率的方法 |
| US8980050B2 (en) | 2012-08-20 | 2015-03-17 | Celanese International Corporation | Methods for removing hemicellulose |
| EP2686434B1 (en) | 2011-03-17 | 2021-07-14 | Danisco US Inc. | Method for reducing viscosity in saccharification process |
| US20150252343A1 (en) * | 2012-10-31 | 2015-09-10 | Danisco Us Inc. | Beta-glucosidase from magnaporthe grisea |
| EP2929021A1 (en) | 2012-12-07 | 2015-10-14 | Danisco US Inc. | Compositions and methods of use |
| BR112015023537B1 (pt) * | 2013-03-15 | 2022-11-29 | Inpex Corporation | Mutante de um microrganismo produtor de celulase,e, método para produção de uma celulase |
| EP3027742B1 (en) | 2013-07-29 | 2019-11-20 | Danisco US Inc. | Variant enzymes |
| FR3014903B1 (fr) * | 2013-12-17 | 2017-12-01 | Ifp Energies Now | Procede d'hydrolyse enzymatique avec production in situ de glycosides hydrolases par des microorganismes genetiquement modifies (mgm) et non mgm |
| TW201527756A (zh) * | 2014-01-10 | 2015-07-16 | Nat Univ Tsing Hua | 提供食品安全地圖之方法、電腦程式產品、和系統 |
| JP6398116B2 (ja) * | 2014-01-22 | 2018-10-03 | 本田技研工業株式会社 | 糖化酵素の生産方法、及び草木類バイオマスの糖化処理方法 |
| JP6398115B2 (ja) * | 2014-01-22 | 2018-10-03 | 本田技研工業株式会社 | 糖化酵素の生産方法、及び草木類バイオマスの糖化処理方法 |
| JP6465470B2 (ja) * | 2014-01-22 | 2019-02-06 | 本田技研工業株式会社 | 糖化酵素の生産方法、及び草木類バイオマスの糖化処理方法 |
| KR20170040246A (ko) | 2014-08-08 | 2017-04-12 | 질레코 인코포레이티드 | 아글리코실화된 효소 및 이의 용도 |
| US20180148683A1 (en) | 2015-07-07 | 2018-05-31 | Danisco Us Inc. | Induction of gene expression using a high concentration sugar mixture |
| DK3419992T3 (da) | 2016-02-22 | 2021-02-15 | Danisco Us Inc | Svampesystem til proteinproduktion med højt udbytte |
| MX391044B (es) * | 2016-04-29 | 2025-03-21 | Novozymes As | Composiciones detergentes y sus usos. |
| WO2018053058A1 (en) | 2016-09-14 | 2018-03-22 | Danisco Us Inc. | Lignocellulosic biomass fermentation-based processes |
| KR102593668B1 (ko) | 2016-10-04 | 2023-10-24 | 다니스코 유에스 인크. | 유도 기질 부재 하의 사상 진균 세포에서의 단백질 생산 |
| RU2771261C2 (ru) | 2016-12-21 | 2022-04-29 | ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС | Способы применения термостабильных сериновых протеаз |
| DK3375884T3 (da) * | 2017-03-15 | 2020-07-27 | Clariant Int Ltd | Fremgangsmåde til fremstilling af proteiner under inducerende betingelser |
| WO2019074828A1 (en) | 2017-10-09 | 2019-04-18 | Danisco Us Inc | CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE |
| MX2022002310A (es) | 2019-08-29 | 2022-06-02 | Danisco Us Inc | Expresión de beta-glucosidasa en levadura para producción mejorada de etanol. |
| CN115125152A (zh) * | 2022-03-28 | 2022-09-30 | 湖南科技学院 | 一种降解木质纤维素的混合菌、混合酶和降解方法 |
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| US5366558A (en) | 1979-03-23 | 1994-11-22 | Brink David L | Method of treating biomass material |
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| US5405769A (en) | 1993-04-08 | 1995-04-11 | National Research Council Of Canada | Construction of thermostable mutants of a low molecular mass xylanase |
| US5705369A (en) | 1994-12-27 | 1998-01-06 | Midwest Research Institute | Prehydrolysis of lignocellulose |
| US5811381A (en) | 1996-10-10 | 1998-09-22 | Mark A. Emalfarb | Cellulase compositions and methods of use |
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| US6409841B1 (en) | 1999-11-02 | 2002-06-25 | Waste Energy Integrated Systems, Llc. | Process for the production of organic products from diverse biomass sources |
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| CN1509330A (zh) | 2001-05-18 | 2004-06-30 | ŵ��ø�ɷ�����˾ | 具有纤维二糖酶活性的多肽和编码其的多核苷酸 |
| US6982159B2 (en) | 2001-09-21 | 2006-01-03 | Genencor International, Inc. | Trichoderma β-glucosidase |
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| AU2003287028B2 (en) | 2002-10-04 | 2008-09-04 | E.I. Du Pont De Nemours And Company | Process for the biological production of 1,3-propanediol with high yield |
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| US7781191B2 (en) | 2005-04-12 | 2010-08-24 | E. I. Du Pont De Nemours And Company | Treatment of biomass to obtain a target chemical |
| JP5149785B2 (ja) | 2005-04-12 | 2013-02-20 | イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー | エタノールを得るためのバイオマスの処理 |
| EP2444487B1 (en) * | 2006-02-10 | 2018-04-18 | BP Corporation North America Inc. | Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them |
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| WO2008153712A2 (en) | 2007-05-21 | 2008-12-18 | Danisco Us, Inc., Genencor Division | Method for introducing nucleic acids into fungal cells |
| BRPI0812267A2 (pt) * | 2007-05-31 | 2014-10-14 | Novozymes Inc | Célula hospedeira fúngica filamentosa, método para produzir uma composição de proteína celulolítica, composição de proteína celulolítica, e, métodos para degradar ou converter um material, contendo celulose e para produzir um produto de fermentação |
| JP5594895B2 (ja) * | 2007-06-06 | 2014-09-24 | ダニスコ・ユーエス・インク、ジェネンコー・ディビジョン | タンパク質の能力の改良方法 |
| CA2698765A1 (en) * | 2007-09-07 | 2009-03-19 | Danisco Us Inc. | Beta-glucosidase enhanced filamentous fungal whole cellulase compositions and methods of use |
| CN101952421A (zh) * | 2007-12-05 | 2011-01-19 | 诺维信公司 | 具有内切葡聚糖酶活性的多肽和编码所述多肽的多核苷酸 |
| BRPI0823506A8 (pt) | 2007-12-13 | 2018-05-15 | Danisco Us Inc Genencor Div | composições e métodos para a produção de isoprene |
| US10676751B2 (en) * | 2008-02-29 | 2020-06-09 | The Trustees Of The University Of Pennsylvania | Production and use of plant degrading materials |
| BRPI1010696A2 (pt) * | 2009-06-16 | 2016-10-11 | Codexis Inc | método de produção de um polipeptídeo de b-glicosidade com termoatividade aperfeiçoada, polipeptídeo, proteína e seus usos |
| JP5641478B2 (ja) * | 2010-07-18 | 2014-12-17 | 独立行政法人国際農林水産業研究センター | 酵素の再利用方法 |
| BR112014004171A2 (pt) * | 2011-08-22 | 2018-11-06 | Codexis Inc | variantes da proteína glicósideo hidrolase gh61 e cofatores que aumentam a atividade de gh61 |
| DK2760885T3 (en) * | 2011-09-30 | 2017-10-30 | Novozymes Inc | CHEMICAL POLYPEPTIDES WITH BETA-GLUCOSIDASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM |
| BR112015009201A2 (pt) * | 2012-10-31 | 2017-11-14 | Danisco Us Inc | composição compreendeendo um polipeptídeo recombinante, ácido nucleico codificando o mesmo, célula hospedeira, bem como métodos para produzir uma beta-glucosidase e para hidrolisar um substrato de biomassa lignocelulósica |
-
2012
- 2012-03-16 EP EP12710854.6A patent/EP2686427A1/en not_active Withdrawn
- 2012-03-16 KR KR1020137027127A patent/KR20140023313A/ko not_active Withdrawn
- 2012-03-16 MX MX2013010509A patent/MX2013010509A/es unknown
- 2012-03-16 SG SG2013056114A patent/SG192097A1/en unknown
- 2012-03-16 CN CN201811241955.0A patent/CN109371002A/zh active Pending
- 2012-03-16 JP JP2013558217A patent/JP6148183B2/ja not_active Expired - Fee Related
- 2012-03-16 US US14/004,872 patent/US20140073017A1/en not_active Abandoned
- 2012-03-16 RU RU2013146341/10A patent/RU2013146341A/ru not_active Application Discontinuation
- 2012-03-16 WO PCT/US2012/029498 patent/WO2012125951A1/en not_active Ceased
- 2012-03-16 BR BR112013023715A patent/BR112013023715A2/pt not_active Application Discontinuation
- 2012-03-16 CN CN201280013801.0A patent/CN103492561A/zh active Pending
- 2012-03-16 AU AU2012228968A patent/AU2012228968B2/en not_active Ceased
- 2012-03-16 CA CA2829918A patent/CA2829918A1/en not_active Abandoned
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2013
- 2013-07-22 ZA ZA2013/05532A patent/ZA201305532B/en unknown
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2017
- 2017-02-23 US US15/440,341 patent/US20180119125A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| WO2012125951A1 (en) | 2012-09-20 |
| KR20140023313A (ko) | 2014-02-26 |
| CN103492561A (zh) | 2014-01-01 |
| BR112013023715A2 (pt) | 2016-09-13 |
| ZA201305532B (en) | 2014-10-29 |
| US20140073017A1 (en) | 2014-03-13 |
| AU2012228968B2 (en) | 2017-05-18 |
| JP6148183B2 (ja) | 2017-06-21 |
| RU2013146341A (ru) | 2015-04-27 |
| CN109371002A (zh) | 2019-02-22 |
| JP2014509858A (ja) | 2014-04-24 |
| EP2686427A1 (en) | 2014-01-22 |
| US20180119125A1 (en) | 2018-05-03 |
| MX2013010509A (es) | 2013-10-17 |
| SG192097A1 (en) | 2013-08-30 |
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