CA2782176C - Methods and apparatus for segregation of particles, including segregation and proliferation of fetal and stem cells - Google Patents

Methods and apparatus for segregation of particles, including segregation and proliferation of fetal and stem cells Download PDF

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Publication number
CA2782176C
CA2782176C CA2782176A CA2782176A CA2782176C CA 2782176 C CA2782176 C CA 2782176C CA 2782176 A CA2782176 A CA 2782176A CA 2782176 A CA2782176 A CA 2782176A CA 2782176 C CA2782176 C CA 2782176C
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CA
Canada
Prior art keywords
cells
passage
particles
fluid
cover
Prior art date
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Active
Application number
CA2782176A
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English (en)
French (fr)
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CA2782176A1 (en
Inventor
George Hvichia
David Counts
Gary Evans
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Angle North America Inc
Original Assignee
Parsortix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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Publication of CA2782176A1 publication Critical patent/CA2782176A1/en
Application granted granted Critical
Publication of CA2782176C publication Critical patent/CA2782176C/en
Active legal-status Critical Current
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Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00Sampling; Preparing specimens for investigation
    • G01N1/28Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40Concentrating samples
    • G01N1/4077Concentrating samples by other techniques involving separation of suspended solids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02Investigating particle size or size distribution
    • G01N15/0272Investigating particle size or size distribution with screening; with classification by filtering
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/49Blood
    • G01N33/491Blood by separating the blood components
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366Apparatus specially adapted for solid-phase testing
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0809Geometry, shape and general structure rectangular shaped
    • B01L2300/0816Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00Additional constructional details
    • B01L2300/08Geometry, shape and general structure
    • B01L2300/0861Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087Multiple sequential chambers
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00Moving or stopping fluids
    • B01L2400/08Regulating or influencing the flow resistance
    • B01L2400/084Passive control of flow resistance
    • B01L2400/086Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Dispersion Chemistry (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Ecology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CA2782176A 2009-11-30 2010-11-29 Methods and apparatus for segregation of particles, including segregation and proliferation of fetal and stem cells Active CA2782176C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US26491809P 2009-11-30 2009-11-30
US61/264,918 2009-11-30
PCT/US2010/058172 WO2011066497A2 (en) 2009-11-30 2010-11-29 Methods and apparatus for segregation of particles, including segregation and proliferation of fetal and stem cells

Publications (2)

Publication Number Publication Date
CA2782176A1 CA2782176A1 (en) 2011-06-03
CA2782176C true CA2782176C (en) 2015-10-06

Family

ID=43332885

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2782176A Active CA2782176C (en) 2009-11-30 2010-11-29 Methods and apparatus for segregation of particles, including segregation and proliferation of fetal and stem cells

Country Status (8)

Country Link
EP (1) EP2507382A4 (ja)
JP (2) JP2013511992A (ja)
CN (1) CN102695804B (ja)
AU (1) AU2010246381B2 (ja)
BR (1) BR112012013034A2 (ja)
CA (1) CA2782176C (ja)
MX (1) MX2012006244A (ja)
WO (1) WO2011066497A2 (ja)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2587248A1 (en) * 2011-10-25 2013-05-01 Koninklijke Philips Electronics N.V. Filtering particles from blood or other media
US9631179B2 (en) 2013-03-15 2017-04-25 Angle North America, Inc. Methods for segregating particles using an apparatus with a size-discriminating separation element having an elongate leading edge
WO2016069624A1 (en) 2014-10-27 2016-05-06 Board Of Regents, The University Of Texas System High-throughput imaging platform
CN105176812B (zh) * 2015-07-28 2017-10-03 浙江奥瑞健生物技术有限公司 一种干细胞球切割收集装置及干细胞球的切割分离传代方法
CA2995474C (en) * 2015-08-18 2022-04-26 National Health Research Institutes A microfluidic hydrodynamic shuttling chip device for highthroughput multiple single cells capture
GB201704766D0 (en) * 2017-01-05 2017-05-10 Illumia Inc System and methods for selective effluent collection
EP3561038A1 (en) * 2018-04-24 2019-10-30 Bayer AG Cell separator
US11389799B2 (en) * 2019-01-17 2022-07-19 The Regents Of The University Of Michigan Microfluidic device for size and deformability measurements and applications thereof
US20220146400A1 (en) * 2019-02-27 2022-05-12 Kyocera Corporation Particle separating and measuring device and particle separating and measuring apparatus
CN109810894B (zh) * 2019-03-01 2022-06-10 长春理工大学 一种基于光诱导介电泳的群细胞三维结构操纵与构建系统及方法
WO2021015089A1 (ja) * 2019-07-23 2021-01-28 国立大学法人佐賀大学 剪断処理による微細藻類の外被破壊法及び外被破壊システム

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008040A (en) * 1995-07-07 1999-12-28 Synosys, Inc. Procedures for efficient separation of cells, cellular materials and proteins
WO2003008931A2 (en) * 2001-07-17 2003-01-30 Georgi Hvichia Microstructure for particle and cell separation, identification, sorting, and manipulation
US6783928B2 (en) * 2001-07-17 2004-08-31 Georgi Hvichia Microstructures for cell proliferation assays and semen analysis
JP2006501449A (ja) * 2002-09-27 2006-01-12 ザ ジェネラル ホスピタル コーポレーション 細胞分離のためのマイクロ流体デバイスおよびその使用
EP1671101A1 (en) * 2003-10-03 2006-06-21 Vrije Universiteit Brussel Method and device for size-separating particles present in a fluid
WO2009131645A2 (en) * 2008-04-23 2009-10-29 Parsortix, Inc. Methods and apparatus for segregation of particles

Also Published As

Publication number Publication date
JP2013511992A (ja) 2013-04-11
AU2010246381B2 (en) 2013-08-15
AU2010246381A1 (en) 2010-12-16
WO2011066497A3 (en) 2011-11-17
CA2782176A1 (en) 2011-06-03
CN102695804B (zh) 2014-09-03
JP2016208978A (ja) 2016-12-15
CN102695804A (zh) 2012-09-26
EP2507382A2 (en) 2012-10-10
BR112012013034A2 (pt) 2016-10-04
WO2011066497A2 (en) 2011-06-03
EP2507382A4 (en) 2016-06-08
MX2012006244A (es) 2012-10-15

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