BR112012013034A2 - métodos e aparelhos para segregação de partículas, incluindo a segregação e a proliferação de células fetais e células-tronco - Google Patents

métodos e aparelhos para segregação de partículas, incluindo a segregação e a proliferação de células fetais e células-tronco

Info

Publication number
BR112012013034A2
BR112012013034A2 BR112012013034A BR112012013034A BR112012013034A2 BR 112012013034 A2 BR112012013034 A2 BR 112012013034A2 BR 112012013034 A BR112012013034 A BR 112012013034A BR 112012013034 A BR112012013034 A BR 112012013034A BR 112012013034 A2 BR112012013034 A2 BR 112012013034A2
Authority
BR
Brazil
Prior art keywords
segregation
particles
methods
proliferation
stem cells
Prior art date
Application number
BR112012013034A
Other languages
English (en)
Inventor
David Counts
Gary Evans
George Hvichia
Original Assignee
Parsortix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Parsortix Inc filed Critical Parsortix Inc
Publication of BR112012013034A2 publication Critical patent/BR112012013034A2/pt

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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/40—Concentrating samples
    • G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/02—Investigating particle size or size distribution
    • G01N15/0272—Investigating particle size or size distribution with screening; with classification by filtering
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/491—Blood by separating the blood components
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/08—Regulating or influencing the flow resistance
    • B01L2400/084—Passive control of flow resistance
    • B01L2400/086—Passive control of flow resistance using baffles or other fixed flow obstructions

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Zoology (AREA)
  • Dispersion Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Clinical Laboratory Science (AREA)
  • Ecology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

métodos e aparelhos para segregação de partículas, incluindo a segregação e a proliferação de células fetais e células-tronco. a divulgação diz respeito a um aparelho para segregar partículas com base na sua capacidade de fluir através de uma passagem intensificada. pelo menos algumas das partículas são acomodadas numa passagem limitada por uma primeira etapa, mas pelo menos algumas das partículas são incapazes de passar através de uma passagem mais estreita delimitada por uma segunda etapa, o que resulta na segregação das partículas. os aparelhos e métodos aqui descritos podem ser usados para segregar partículas de uma grande variedade de tipos. a título de exemplo, eles podem ser usados para segregar células fetais semelhantes a partir de uma amostra de sangue materno, tais como sangue arterial materno.
BR112012013034A 2009-11-30 2010-11-29 métodos e aparelhos para segregação de partículas, incluindo a segregação e a proliferação de células fetais e células-tronco BR112012013034A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US26491809P 2009-11-30 2009-11-30
PCT/US2010/058172 WO2011066497A2 (en) 2009-11-30 2010-11-29 Methods and apparatus for segregation of particles, including segregation and proliferation of fetal and stem cells

Publications (1)

Publication Number Publication Date
BR112012013034A2 true BR112012013034A2 (pt) 2016-10-04

Family

ID=43332885

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112012013034A BR112012013034A2 (pt) 2009-11-30 2010-11-29 métodos e aparelhos para segregação de partículas, incluindo a segregação e a proliferação de células fetais e células-tronco

Country Status (8)

Country Link
EP (1) EP2507382A4 (pt)
JP (2) JP2013511992A (pt)
CN (1) CN102695804B (pt)
AU (1) AU2010246381B2 (pt)
BR (1) BR112012013034A2 (pt)
CA (1) CA2782176C (pt)
MX (1) MX2012006244A (pt)
WO (1) WO2011066497A2 (pt)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2587248A1 (en) * 2011-10-25 2013-05-01 Koninklijke Philips Electronics N.V. Filtering particles from blood or other media
US9631179B2 (en) 2013-03-15 2017-04-25 Angle North America, Inc. Methods for segregating particles using an apparatus with a size-discriminating separation element having an elongate leading edge
WO2016069624A1 (en) 2014-10-27 2016-05-06 Board Of Regents, The University Of Texas System High-throughput imaging platform
CN105176812B (zh) * 2015-07-28 2017-10-03 浙江奥瑞健生物技术有限公司 一种干细胞球切割收集装置及干细胞球的切割分离传代方法
CA2995474C (en) * 2015-08-18 2022-04-26 National Health Research Institutes A microfluidic hydrodynamic shuttling chip device for highthroughput multiple single cells capture
GB201704766D0 (en) * 2017-01-05 2017-05-10 Illumia Inc System and methods for selective effluent collection
EP3561038A1 (en) * 2018-04-24 2019-10-30 Bayer AG Cell separator
US11389799B2 (en) * 2019-01-17 2022-07-19 The Regents Of The University Of Michigan Microfluidic device for size and deformability measurements and applications thereof
CN113454437B (zh) * 2019-02-27 2024-03-15 京瓷株式会社 粒子分离器件以及粒子分离计测装置
CN109810894B (zh) * 2019-03-01 2022-06-10 长春理工大学 一种基于光诱导介电泳的群细胞三维结构操纵与构建系统及方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6008040A (en) * 1995-07-07 1999-12-28 Synosys, Inc. Procedures for efficient separation of cells, cellular materials and proteins
AU2002320576A1 (en) * 2001-07-17 2003-03-03 Paolo Gasparini Microstructure for particle and cell separation, identification, sorting, and manipulation
US6783928B2 (en) * 2001-07-17 2004-08-31 Georgi Hvichia Microstructures for cell proliferation assays and semen analysis
EP2359689B1 (en) * 2002-09-27 2015-08-26 The General Hospital Corporation Microfluidic device for cell separation and use thereof
US20070039463A1 (en) * 2003-10-02 2007-02-22 Gert Desmet Method and device for size-separating particles present in a fluid
CN102083997B (zh) * 2008-04-23 2016-05-25 角度北美公司 用于分离颗粒的方法和设备

Also Published As

Publication number Publication date
CN102695804B (zh) 2014-09-03
JP2016208978A (ja) 2016-12-15
EP2507382A2 (en) 2012-10-10
CN102695804A (zh) 2012-09-26
EP2507382A4 (en) 2016-06-08
WO2011066497A2 (en) 2011-06-03
CA2782176A1 (en) 2011-06-03
AU2010246381A1 (en) 2010-12-16
MX2012006244A (es) 2012-10-15
AU2010246381B2 (en) 2013-08-15
WO2011066497A3 (en) 2011-11-17
CA2782176C (en) 2015-10-06
JP2013511992A (ja) 2013-04-11

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Legal Events

Date Code Title Description
B08F Application fees: application dismissed [chapter 8.6 patent gazette]
B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]