CA2775093A1 - Down-regulation of acc synthase for improved plant performance - Google Patents
Down-regulation of acc synthase for improved plant performance Download PDFInfo
- Publication number
- CA2775093A1 CA2775093A1 CA2775093A CA2775093A CA2775093A1 CA 2775093 A1 CA2775093 A1 CA 2775093A1 CA 2775093 A CA2775093 A CA 2775093A CA 2775093 A CA2775093 A CA 2775093A CA 2775093 A1 CA2775093 A1 CA 2775093A1
- Authority
- CA
- Canada
- Prior art keywords
- plant
- expression
- seq
- plants
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 230000003828 downregulation Effects 0.000 title claims abstract description 63
- 230000001976 improved effect Effects 0.000 title abstract description 30
- 101710194665 1-aminocyclopropane-1-carboxylate synthase Proteins 0.000 claims abstract description 175
- 238000000034 method Methods 0.000 claims abstract description 150
- 230000009261 transgenic effect Effects 0.000 claims abstract description 70
- VGGSQFUCUMXWEO-UHFFFAOYSA-N Ethene Chemical compound C=C VGGSQFUCUMXWEO-UHFFFAOYSA-N 0.000 claims abstract description 55
- 239000005977 Ethylene Substances 0.000 claims abstract description 55
- 238000004519 manufacturing process Methods 0.000 claims abstract description 41
- 230000001965 increasing effect Effects 0.000 claims abstract description 38
- 230000009467 reduction Effects 0.000 claims abstract description 29
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 21
- 239000002028 Biomass Substances 0.000 claims abstract description 17
- 230000014075 nitrogen utilization Effects 0.000 claims abstract description 14
- 241000196324 Embryophyta Species 0.000 claims description 547
- 230000014509 gene expression Effects 0.000 claims description 209
- 108090000623 proteins and genes Proteins 0.000 claims description 163
- 150000007523 nucleic acids Chemical class 0.000 claims description 128
- 102000040430 polynucleotide Human genes 0.000 claims description 116
- 108091033319 polynucleotide Proteins 0.000 claims description 116
- 239000002157 polynucleotide Substances 0.000 claims description 116
- 102000039446 nucleic acids Human genes 0.000 claims description 103
- 108020004707 nucleic acids Proteins 0.000 claims description 103
- 240000008042 Zea mays Species 0.000 claims description 92
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 73
- 230000000694 effects Effects 0.000 claims description 67
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 63
- 235000009973 maize Nutrition 0.000 claims description 63
- 239000002773 nucleotide Substances 0.000 claims description 41
- 125000003729 nucleotide group Chemical group 0.000 claims description 41
- 101100433501 Arabidopsis thaliana ACS6 gene Proteins 0.000 claims description 32
- 230000024346 drought recovery Effects 0.000 claims description 32
- 230000002829 reductive effect Effects 0.000 claims description 28
- 230000009368 gene silencing by RNA Effects 0.000 claims description 19
- 240000007594 Oryza sativa Species 0.000 claims description 18
- 235000007164 Oryza sativa Nutrition 0.000 claims description 18
- 235000009566 rice Nutrition 0.000 claims description 16
- 244000068988 Glycine max Species 0.000 claims description 15
- 235000010469 Glycine max Nutrition 0.000 claims description 14
- 108020004999 messenger RNA Proteins 0.000 claims description 14
- 241000209510 Liliopsida Species 0.000 claims description 10
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 9
- 241000209140 Triticum Species 0.000 claims description 9
- 235000021307 Triticum Nutrition 0.000 claims description 9
- 230000006870 function Effects 0.000 claims description 9
- 235000007340 Hordeum vulgare Nutrition 0.000 claims description 8
- 235000011684 Sorghum saccharatum Nutrition 0.000 claims description 8
- 230000001603 reducing effect Effects 0.000 claims description 8
- 241001233957 eudicotyledons Species 0.000 claims description 7
- 244000075850 Avena orientalis Species 0.000 claims description 6
- 244000020551 Helianthus annuus Species 0.000 claims description 5
- 235000003222 Helianthus annuus Nutrition 0.000 claims description 5
- 235000007319 Avena orientalis Nutrition 0.000 claims description 4
- 235000007238 Secale cereale Nutrition 0.000 claims description 4
- 101150047711 acs gene Proteins 0.000 claims description 4
- 235000011331 Brassica Nutrition 0.000 claims description 3
- 241000219198 Brassica Species 0.000 claims description 3
- 241001520823 Zoysia Species 0.000 claims description 3
- 230000002222 downregulating effect Effects 0.000 claims description 2
- 240000006394 Sorghum bicolor Species 0.000 claims 3
- 235000007558 Avena sp Nutrition 0.000 claims 2
- 240000005979 Hordeum vulgare Species 0.000 claims 2
- 244000082988 Secale cereale Species 0.000 claims 2
- 239000013598 vector Substances 0.000 abstract description 54
- 230000036579 abiotic stress Effects 0.000 abstract description 12
- 210000004027 cell Anatomy 0.000 description 169
- 108090000765 processed proteins & peptides Proteins 0.000 description 111
- 102000004196 processed proteins & peptides Human genes 0.000 description 99
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 98
- 229920001184 polypeptide Polymers 0.000 description 97
- 210000001519 tissue Anatomy 0.000 description 69
- 108020004414 DNA Proteins 0.000 description 56
- 102000004169 proteins and genes Human genes 0.000 description 56
- 229910052757 nitrogen Inorganic materials 0.000 description 53
- 235000018102 proteins Nutrition 0.000 description 53
- 230000009466 transformation Effects 0.000 description 51
- 210000002257 embryonic structure Anatomy 0.000 description 50
- 239000002609 medium Substances 0.000 description 42
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 40
- 239000002245 particle Substances 0.000 description 37
- 230000035882 stress Effects 0.000 description 34
- 239000013612 plasmid Substances 0.000 description 32
- 235000001014 amino acid Nutrition 0.000 description 30
- 229940024606 amino acid Drugs 0.000 description 29
- 150000001413 amino acids Chemical class 0.000 description 29
- 238000009396 hybridization Methods 0.000 description 26
- 238000013518 transcription Methods 0.000 description 26
- 230000035897 transcription Effects 0.000 description 26
- 108091027967 Small hairpin RNA Proteins 0.000 description 24
- 239000000047 product Substances 0.000 description 24
- 241000589158 Agrobacterium Species 0.000 description 23
- 230000000692 anti-sense effect Effects 0.000 description 23
- 230000012010 growth Effects 0.000 description 23
- 230000008929 regeneration Effects 0.000 description 23
- 238000011069 regeneration method Methods 0.000 description 23
- 230000000295 complement effect Effects 0.000 description 21
- PAJPWUMXBYXFCZ-UHFFFAOYSA-N 1-aminocyclopropanecarboxylic acid Chemical compound OC(=O)C1(N)CC1 PAJPWUMXBYXFCZ-UHFFFAOYSA-N 0.000 description 20
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 20
- 108091026890 Coding region Proteins 0.000 description 19
- 235000013339 cereals Nutrition 0.000 description 19
- 241000894007 species Species 0.000 description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 18
- 101000773184 Homo sapiens Twist-related protein 1 Proteins 0.000 description 17
- 230000015572 biosynthetic process Effects 0.000 description 17
- 230000001404 mediated effect Effects 0.000 description 17
- 239000000243 solution Substances 0.000 description 17
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 16
- 102100034334 Fatty acid CoA ligase Acsl3 Human genes 0.000 description 16
- 101000780194 Homo sapiens Fatty acid CoA ligase Acsl3 Proteins 0.000 description 16
- 108700019146 Transgenes Proteins 0.000 description 16
- 230000000408 embryogenic effect Effects 0.000 description 16
- 239000003550 marker Substances 0.000 description 16
- 239000000203 mixture Substances 0.000 description 15
- 108020004705 Codon Proteins 0.000 description 14
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 14
- 125000003275 alpha amino acid group Chemical group 0.000 description 14
- 238000011161 development Methods 0.000 description 14
- 239000013604 expression vector Substances 0.000 description 14
- 238000006467 substitution reaction Methods 0.000 description 14
- 206010020649 Hyperkeratosis Diseases 0.000 description 13
- 230000001105 regulatory effect Effects 0.000 description 13
- 239000000523 sample Substances 0.000 description 13
- 230000001629 suppression Effects 0.000 description 13
- 239000000725 suspension Substances 0.000 description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 12
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 12
- 238000007792 addition Methods 0.000 description 12
- 238000003556 assay Methods 0.000 description 12
- 230000018109 developmental process Effects 0.000 description 12
- 230000002068 genetic effect Effects 0.000 description 12
- 230000001939 inductive effect Effects 0.000 description 12
- 239000007788 liquid Substances 0.000 description 12
- 210000001938 protoplast Anatomy 0.000 description 12
- 238000012546 transfer Methods 0.000 description 12
- 229910001868 water Inorganic materials 0.000 description 12
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 11
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 11
- 244000062793 Sorghum vulgare Species 0.000 description 11
- 239000002689 soil Substances 0.000 description 11
- 238000003786 synthesis reaction Methods 0.000 description 11
- 238000013519 translation Methods 0.000 description 11
- 108700001094 Plant Genes Proteins 0.000 description 10
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 10
- 230000004075 alteration Effects 0.000 description 10
- 238000009395 breeding Methods 0.000 description 10
- 235000005822 corn Nutrition 0.000 description 10
- 230000035772 mutation Effects 0.000 description 10
- 210000000056 organ Anatomy 0.000 description 10
- 230000032361 posttranscriptional gene silencing Effects 0.000 description 10
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 10
- 238000003259 recombinant expression Methods 0.000 description 10
- 230000010076 replication Effects 0.000 description 10
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 10
- 102000004190 Enzymes Human genes 0.000 description 9
- 108090000790 Enzymes Proteins 0.000 description 9
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 9
- 238000004458 analytical method Methods 0.000 description 9
- 230000027455 binding Effects 0.000 description 9
- 230000001488 breeding effect Effects 0.000 description 9
- 238000004113 cell culture Methods 0.000 description 9
- 239000002299 complementary DNA Substances 0.000 description 9
- 238000001514 detection method Methods 0.000 description 9
- 229940088598 enzyme Drugs 0.000 description 9
- 239000012634 fragment Substances 0.000 description 9
- 235000019333 sodium laurylsulphate Nutrition 0.000 description 9
- 230000000392 somatic effect Effects 0.000 description 9
- 239000006228 supernatant Substances 0.000 description 9
- 230000001131 transforming effect Effects 0.000 description 9
- 241000894006 Bacteria Species 0.000 description 8
- 108700010070 Codon Usage Proteins 0.000 description 8
- 241000209219 Hordeum Species 0.000 description 8
- 238000012228 RNA interference-mediated gene silencing Methods 0.000 description 8
- MEFKEPWMEQBLKI-AIRLBKTGSA-N S-adenosyl-L-methioninate Chemical compound O[C@@H]1[C@H](O)[C@@H](C[S+](CC[C@H](N)C([O-])=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 MEFKEPWMEQBLKI-AIRLBKTGSA-N 0.000 description 8
- 229930006000 Sucrose Natural products 0.000 description 8
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 8
- 230000003321 amplification Effects 0.000 description 8
- 239000002585 base Substances 0.000 description 8
- 239000004009 herbicide Substances 0.000 description 8
- 230000006872 improvement Effects 0.000 description 8
- 239000000463 material Substances 0.000 description 8
- 238000012544 monitoring process Methods 0.000 description 8
- 239000000618 nitrogen fertilizer Substances 0.000 description 8
- 238000003199 nucleic acid amplification method Methods 0.000 description 8
- 230000010152 pollination Effects 0.000 description 8
- 150000003839 salts Chemical class 0.000 description 8
- 239000005720 sucrose Substances 0.000 description 8
- 238000011282 treatment Methods 0.000 description 8
- 108020003589 5' Untranslated Regions Proteins 0.000 description 7
- 108010000700 Acetolactate synthase Proteins 0.000 description 7
- 241000589156 Agrobacterium rhizogenes Species 0.000 description 7
- 125000000539 amino acid group Chemical group 0.000 description 7
- 238000006243 chemical reaction Methods 0.000 description 7
- 239000003795 chemical substances by application Substances 0.000 description 7
- 238000004520 electroporation Methods 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 7
- 230000007613 environmental effect Effects 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- 230000008488 polyadenylation Effects 0.000 description 7
- 239000000843 powder Substances 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- 238000000527 sonication Methods 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 6
- 241000219194 Arabidopsis Species 0.000 description 6
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 6
- 241000219823 Medicago Species 0.000 description 6
- 108700026244 Open Reading Frames Proteins 0.000 description 6
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 6
- 230000003115 biocidal effect Effects 0.000 description 6
- 238000010367 cloning Methods 0.000 description 6
- 230000007423 decrease Effects 0.000 description 6
- 230000008641 drought stress Effects 0.000 description 6
- 238000011156 evaluation Methods 0.000 description 6
- 230000001747 exhibiting effect Effects 0.000 description 6
- 230000002363 herbicidal effect Effects 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- 230000000670 limiting effect Effects 0.000 description 6
- 230000008121 plant development Effects 0.000 description 6
- 229920000642 polymer Polymers 0.000 description 6
- 238000003752 polymerase chain reaction Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 230000001568 sexual effect Effects 0.000 description 6
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 6
- 239000011780 sodium chloride Substances 0.000 description 6
- ATHGHQPFGPMSJY-UHFFFAOYSA-N spermidine Chemical compound NCCCCNCCCN ATHGHQPFGPMSJY-UHFFFAOYSA-N 0.000 description 6
- 108091093088 Amplicon Proteins 0.000 description 5
- 241000209200 Bromus Species 0.000 description 5
- 241000701489 Cauliflower mosaic virus Species 0.000 description 5
- 229920002148 Gellan gum Polymers 0.000 description 5
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 5
- 241000227653 Lycopersicon Species 0.000 description 5
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 5
- IAJOBQBIJHVGMQ-UHFFFAOYSA-N Phosphinothricin Natural products CP(O)(=O)CCC(N)C(O)=O IAJOBQBIJHVGMQ-UHFFFAOYSA-N 0.000 description 5
- 108010076504 Protein Sorting Signals Proteins 0.000 description 5
- 108090000848 Ubiquitin Proteins 0.000 description 5
- 102000044159 Ubiquitin Human genes 0.000 description 5
- 108091023045 Untranslated Region Proteins 0.000 description 5
- 235000007244 Zea mays Nutrition 0.000 description 5
- 238000004422 calculation algorithm Methods 0.000 description 5
- 229930002875 chlorophyll Natural products 0.000 description 5
- 235000019804 chlorophyll Nutrition 0.000 description 5
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 5
- 238000012217 deletion Methods 0.000 description 5
- 230000037430 deletion Effects 0.000 description 5
- 235000013399 edible fruits Nutrition 0.000 description 5
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 5
- 229910052737 gold Inorganic materials 0.000 description 5
- 239000010931 gold Substances 0.000 description 5
- 238000003306 harvesting Methods 0.000 description 5
- 238000002955 isolation Methods 0.000 description 5
- 239000012528 membrane Substances 0.000 description 5
- 229930182817 methionine Natural products 0.000 description 5
- 229960004452 methionine Drugs 0.000 description 5
- 108010058731 nopaline synthase Proteins 0.000 description 5
- 238000003976 plant breeding Methods 0.000 description 5
- 235000010333 potassium nitrate Nutrition 0.000 description 5
- 239000013615 primer Substances 0.000 description 5
- 230000000644 propagated effect Effects 0.000 description 5
- 238000000746 purification Methods 0.000 description 5
- 238000011160 research Methods 0.000 description 5
- 238000004114 suspension culture Methods 0.000 description 5
- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 4
- 108010010888 1-aminocyclopropane-1-carboxylic acid oxidase Proteins 0.000 description 4
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 4
- 229920000742 Cotton Polymers 0.000 description 4
- 241001303487 Digitaria <clam> Species 0.000 description 4
- 241000588724 Escherichia coli Species 0.000 description 4
- 244000299507 Gossypium hirsutum Species 0.000 description 4
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 4
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 4
- 241000209056 Secale Species 0.000 description 4
- 241000700605 Viruses Species 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 239000001110 calcium chloride Substances 0.000 description 4
- 229910001628 calcium chloride Inorganic materials 0.000 description 4
- 235000011148 calcium chloride Nutrition 0.000 description 4
- 230000008859 change Effects 0.000 description 4
- 210000000349 chromosome Anatomy 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- -1 e.g. Substances 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000011536 extraction buffer Substances 0.000 description 4
- 108020002326 glutamine synthetase Proteins 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000001307 helium Substances 0.000 description 4
- 229910052734 helium Inorganic materials 0.000 description 4
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 4
- 208000000509 infertility Diseases 0.000 description 4
- 230000036512 infertility Effects 0.000 description 4
- 208000021267 infertility disease Diseases 0.000 description 4
- 230000000977 initiatory effect Effects 0.000 description 4
- 238000007834 ligase chain reaction Methods 0.000 description 4
- 238000002844 melting Methods 0.000 description 4
- 230000008018 melting Effects 0.000 description 4
- 235000019713 millet Nutrition 0.000 description 4
- 239000003921 oil Substances 0.000 description 4
- 230000037361 pathway Effects 0.000 description 4
- 239000008188 pellet Substances 0.000 description 4
- 238000002360 preparation method Methods 0.000 description 4
- 230000006798 recombination Effects 0.000 description 4
- 238000005215 recombination Methods 0.000 description 4
- 238000012552 review Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- AVWQQPYHYQKEIZ-UHFFFAOYSA-K trisodium;2-dodecylbenzenesulfonate;3-dodecylbenzenesulfonate;4-dodecylbenzenesulfonate Chemical compound [Na+].[Na+].[Na+].CCCCCCCCCCCCC1=CC=C(S([O-])(=O)=O)C=C1.CCCCCCCCCCCCC1=CC=CC(S([O-])(=O)=O)=C1.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O AVWQQPYHYQKEIZ-UHFFFAOYSA-K 0.000 description 4
- WFKWXMTUELFFGS-UHFFFAOYSA-N tungsten Chemical compound [W] WFKWXMTUELFFGS-UHFFFAOYSA-N 0.000 description 4
- 229910052721 tungsten Inorganic materials 0.000 description 4
- 239000010937 tungsten Substances 0.000 description 4
- 229940088594 vitamin Drugs 0.000 description 4
- 239000011782 vitamin Substances 0.000 description 4
- 235000013343 vitamin Nutrition 0.000 description 4
- 229930003231 vitamin Natural products 0.000 description 4
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 description 3
- ZBMRKNMTMPPMMK-UHFFFAOYSA-N 2-amino-4-[hydroxy(methyl)phosphoryl]butanoic acid;azane Chemical compound [NH4+].CP(O)(=O)CCC(N)C([O-])=O ZBMRKNMTMPPMMK-UHFFFAOYSA-N 0.000 description 3
- 102100034035 Alcohol dehydrogenase 1A Human genes 0.000 description 3
- 241000219195 Arabidopsis thaliana Species 0.000 description 3
- 235000014698 Brassica juncea var multisecta Nutrition 0.000 description 3
- 235000006008 Brassica napus var napus Nutrition 0.000 description 3
- 235000006618 Brassica rapa subsp oleifera Nutrition 0.000 description 3
- 244000188595 Brassica sinapistrum Species 0.000 description 3
- 235000002566 Capsicum Nutrition 0.000 description 3
- 108090000994 Catalytic RNA Proteins 0.000 description 3
- 102000053642 Catalytic RNA Human genes 0.000 description 3
- 241000218631 Coniferophyta Species 0.000 description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 241000206602 Eukaryota Species 0.000 description 3
- 108700007698 Genetic Terminator Regions Proteins 0.000 description 3
- 101000892220 Geobacillus thermodenitrificans (strain NG80-2) Long-chain-alcohol dehydrogenase 1 Proteins 0.000 description 3
- 101000780443 Homo sapiens Alcohol dehydrogenase 1A Proteins 0.000 description 3
- 241000700189 Hystrix <Rodentia> Species 0.000 description 3
- 206010021929 Infertility male Diseases 0.000 description 3
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 3
- 108091026898 Leader sequence (mRNA) Proteins 0.000 description 3
- 208000007466 Male Infertility Diseases 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108091034117 Oligonucleotide Proteins 0.000 description 3
- 108091030071 RNAI Proteins 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 244000061456 Solanum tuberosum Species 0.000 description 3
- 235000002595 Solanum tuberosum Nutrition 0.000 description 3
- 241000219793 Trifolium Species 0.000 description 3
- 241000219977 Vigna Species 0.000 description 3
- 230000001133 acceleration Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 230000009418 agronomic effect Effects 0.000 description 3
- 235000004279 alanine Nutrition 0.000 description 3
- 101150103518 bar gene Proteins 0.000 description 3
- GINJFDRNADDBIN-FXQIFTODSA-N bilanafos Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CCP(C)(O)=O GINJFDRNADDBIN-FXQIFTODSA-N 0.000 description 3
- 230000004071 biological effect Effects 0.000 description 3
- 239000000872 buffer Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000011284 combination treatment Methods 0.000 description 3
- 239000003184 complementary RNA Substances 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 235000021186 dishes Nutrition 0.000 description 3
- 230000008030 elimination Effects 0.000 description 3
- 238000003379 elimination reaction Methods 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- IAJOBQBIJHVGMQ-BYPYZUCNSA-N glufosinate-P Chemical compound CP(O)(=O)CC[C@H](N)C(O)=O IAJOBQBIJHVGMQ-BYPYZUCNSA-N 0.000 description 3
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 3
- 102000005396 glutamine synthetase Human genes 0.000 description 3
- 108010002685 hygromycin-B kinase Proteins 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 208000015181 infectious disease Diseases 0.000 description 3
- 108010083942 mannopine synthase Proteins 0.000 description 3
- 238000005259 measurement Methods 0.000 description 3
- MYWUZJCMWCOHBA-VIFPVBQESA-N methamphetamine Chemical compound CN[C@@H](C)CC1=CC=CC=C1 MYWUZJCMWCOHBA-VIFPVBQESA-N 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 238000000520 microinjection Methods 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- 230000002018 overexpression Effects 0.000 description 3
- 230000036961 partial effect Effects 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 108010082527 phosphinothricin N-acetyltransferase Proteins 0.000 description 3
- 230000029553 photosynthesis Effects 0.000 description 3
- 238000010672 photosynthesis Methods 0.000 description 3
- 230000000243 photosynthetic effect Effects 0.000 description 3
- 239000013600 plasmid vector Substances 0.000 description 3
- 210000002706 plastid Anatomy 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 210000001236 prokaryotic cell Anatomy 0.000 description 3
- 230000001850 reproductive effect Effects 0.000 description 3
- 230000003938 response to stress Effects 0.000 description 3
- 230000000284 resting effect Effects 0.000 description 3
- 108091092562 ribozyme Proteins 0.000 description 3
- 230000021749 root development Effects 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 239000006152 selective media Substances 0.000 description 3
- 239000004460 silage Substances 0.000 description 3
- 229940063673 spermidine Drugs 0.000 description 3
- 238000007619 statistical method Methods 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 230000005026 transcription initiation Effects 0.000 description 3
- 230000002103 transcriptional effect Effects 0.000 description 3
- 238000011426 transformation method Methods 0.000 description 3
- OVSKIKFHRZPJSS-DOMIDYPGSA-N 2-(2,4-dichlorophenoxy)acetic acid Chemical compound OC(=O)[14CH2]OC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-DOMIDYPGSA-N 0.000 description 2
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241000234282 Allium Species 0.000 description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- 108020005544 Antisense RNA Proteins 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 241000208838 Asteraceae Species 0.000 description 2
- 235000005781 Avena Nutrition 0.000 description 2
- 241000848284 Avenella Species 0.000 description 2
- 241000560364 Avenula Species 0.000 description 2
- 241000743774 Brachypodium Species 0.000 description 2
- 240000007124 Brassica oleracea Species 0.000 description 2
- 235000003899 Brassica oleracea var acephala Nutrition 0.000 description 2
- 235000011301 Brassica oleracea var capitata Nutrition 0.000 description 2
- 235000001169 Brassica oleracea var oleracea Nutrition 0.000 description 2
- 241001551143 Catapodium Species 0.000 description 2
- 102000053602 DNA Human genes 0.000 description 2
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 2
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 2
- 241000142836 Danthonia Species 0.000 description 2
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 2
- 241000744312 Ehrharta Species 0.000 description 2
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 2
- 241000220485 Fabaceae Species 0.000 description 2
- 241000234642 Festuca Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 239000005561 Glufosinate Substances 0.000 description 2
- 241001523358 Glyceria Species 0.000 description 2
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 2
- 239000005562 Glyphosate Substances 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- 241001551113 Hainardia Species 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 2
- 108091092195 Intron Proteins 0.000 description 2
- 239000007836 KH2PO4 Substances 0.000 description 2
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 2
- QIVBCDIJIAJPQS-VIFPVBQESA-N L-tryptophane Chemical compound C1=CC=C2C(C[C@H](N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-VIFPVBQESA-N 0.000 description 2
- 241000209082 Lolium Species 0.000 description 2
- 239000004472 Lysine Substances 0.000 description 2
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 241000044446 Melinis Species 0.000 description 2
- 241001465754 Metazoa Species 0.000 description 2
- 108020005196 Mitochondrial DNA Proteins 0.000 description 2
- 241000320642 Monanthochloe Species 0.000 description 2
- 229910002651 NO3 Inorganic materials 0.000 description 2
- 240000002853 Nelumbo nucifera Species 0.000 description 2
- 235000006508 Nelumbo nucifera Nutrition 0.000 description 2
- 235000006510 Nelumbo pentapetala Nutrition 0.000 description 2
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 description 2
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 241000209094 Oryza Species 0.000 description 2
- 241000209046 Pennisetum Species 0.000 description 2
- 239000006002 Pepper Substances 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000016761 Piper aduncum Nutrition 0.000 description 2
- 240000003889 Piper guineense Species 0.000 description 2
- 235000017804 Piper guineense Nutrition 0.000 description 2
- 235000008184 Piper nigrum Nutrition 0.000 description 2
- 241000219843 Pisum Species 0.000 description 2
- 241001523344 Pseudoroegneria Species 0.000 description 2
- 108091028664 Ribonucleotide Proteins 0.000 description 2
- 108010003581 Ribulose-bisphosphate carboxylase Proteins 0.000 description 2
- 241001475959 Rostraria Species 0.000 description 2
- 240000000111 Saccharum officinarum Species 0.000 description 2
- 235000007201 Saccharum officinarum Nutrition 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 235000007230 Sorghum bicolor Nutrition 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 241000052184 Swallenia Species 0.000 description 2
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 2
- 108091036066 Three prime untranslated region Proteins 0.000 description 2
- 102000003929 Transaminases Human genes 0.000 description 2
- 108090000340 Transaminases Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- QIVBCDIJIAJPQS-UHFFFAOYSA-N Tryptophan Natural products C1=CC=C2C(CC(N)C(O)=O)=CNC2=C1 QIVBCDIJIAJPQS-UHFFFAOYSA-N 0.000 description 2
- 235000010726 Vigna sinensis Nutrition 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 229960001570 ademetionine Drugs 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000011543 agarose gel Substances 0.000 description 2
- 238000005054 agglomeration Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 238000000540 analysis of variance Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000006696 biosynthetic metabolic pathway Effects 0.000 description 2
- 230000004790 biotic stress Effects 0.000 description 2
- 230000001276 controlling effect Effects 0.000 description 2
- 238000005520 cutting process Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 210000005069 ears Anatomy 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 244000037666 field crops Species 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 2
- 230000004077 genetic alteration Effects 0.000 description 2
- 231100000118 genetic alteration Toxicity 0.000 description 2
- BRZYSWJRSDMWLG-CAXSIQPQSA-N geneticin Chemical compound O1C[C@@](O)(C)[C@H](NC)[C@@H](O)[C@H]1O[C@@H]1[C@@H](O)[C@H](O[C@@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](C(C)O)O2)N)[C@@H](N)C[C@H]1N BRZYSWJRSDMWLG-CAXSIQPQSA-N 0.000 description 2
- 239000012869 germination medium Substances 0.000 description 2
- XDDAORKBJWWYJS-UHFFFAOYSA-N glyphosate Chemical compound OC(=O)CNCP(O)(O)=O XDDAORKBJWWYJS-UHFFFAOYSA-N 0.000 description 2
- 229940097068 glyphosate Drugs 0.000 description 2
- 108010039239 glyphosate N-acetyltransferase Proteins 0.000 description 2
- WQYVRQLZKVEZGA-UHFFFAOYSA-N hypochlorite Chemical compound Cl[O-] WQYVRQLZKVEZGA-UHFFFAOYSA-N 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 238000010348 incorporation Methods 0.000 description 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 2
- 230000006698 induction Effects 0.000 description 2
- 239000003112 inhibitor Substances 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000011081 inoculation Methods 0.000 description 2
- 229960000367 inositol Drugs 0.000 description 2
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 description 2
- 210000004962 mammalian cell Anatomy 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 238000007826 nucleic acid assay Methods 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 101150113864 pat gene Proteins 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000008447 perception Effects 0.000 description 2
- YBYRMVIVWMBXKQ-UHFFFAOYSA-N phenylmethanesulfonyl fluoride Chemical compound FS(=O)(=O)CC1=CC=CC=C1 YBYRMVIVWMBXKQ-UHFFFAOYSA-N 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 230000008640 plant stress response Effects 0.000 description 2
- 238000004161 plant tissue culture Methods 0.000 description 2
- 239000005014 poly(hydroxyalkanoate) Substances 0.000 description 2
- 229920000903 polyhydroxyalkanoate Polymers 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 229960002429 proline Drugs 0.000 description 2
- 238000002731 protein assay Methods 0.000 description 2
- 238000000164 protein isolation Methods 0.000 description 2
- NGVDGCNFYWLIFO-UHFFFAOYSA-N pyridoxal 5'-phosphate Chemical compound CC1=NC=C(COP(O)(O)=O)C(C=O)=C1O NGVDGCNFYWLIFO-UHFFFAOYSA-N 0.000 description 2
- 238000010839 reverse transcription Methods 0.000 description 2
- 239000002336 ribonucleotide Substances 0.000 description 2
- 125000002652 ribonucleotide group Chemical group 0.000 description 2
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 description 2
- 230000009758 senescence Effects 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 230000030118 somatic embryogenesis Effects 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 239000007858 starting material Substances 0.000 description 2
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 2
- 108010008664 streptomycin 3''-kinase Proteins 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- FRGKKTITADJNOE-UHFFFAOYSA-N sulfanyloxyethane Chemical compound CCOS FRGKKTITADJNOE-UHFFFAOYSA-N 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 235000019157 thiamine Nutrition 0.000 description 2
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 2
- 229960003495 thiamine Drugs 0.000 description 2
- 239000011721 thiamine Substances 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 230000014621 translational initiation Effects 0.000 description 2
- 230000017105 transposition Effects 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 101150085703 vir gene Proteins 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- 241000228158 x Triticosecale Species 0.000 description 2
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 108020005345 3' Untranslated Regions Proteins 0.000 description 1
- 102100026105 3-ketoacyl-CoA thiolase, mitochondrial Human genes 0.000 description 1
- OSJPPGNTCRNQQC-UWTATZPHSA-N 3-phospho-D-glyceric acid Chemical compound OC(=O)[C@H](O)COP(O)(O)=O OSJPPGNTCRNQQC-UWTATZPHSA-N 0.000 description 1
- 108010020183 3-phosphoshikimate 1-carboxyvinyltransferase Proteins 0.000 description 1
- GNKZMNRKLCTJAY-UHFFFAOYSA-N 4'-Methylacetophenone Chemical compound CC(=O)C1=CC=C(C)C=C1 GNKZMNRKLCTJAY-UHFFFAOYSA-N 0.000 description 1
- WUUGFSXJNOTRMR-IOSLPCCCSA-N 5'-S-methyl-5'-thioadenosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CSC)O[C@H]1N1C2=NC=NC(N)=C2N=C1 WUUGFSXJNOTRMR-IOSLPCCCSA-N 0.000 description 1
- 101150011812 AADAC gene Proteins 0.000 description 1
- 101150031979 ACS6 gene Proteins 0.000 description 1
- WFPZSXYXPSUOPY-ROYWQJLOSA-N ADP alpha-D-glucoside Chemical compound C([C@H]1O[C@H]([C@@H]([C@@H]1O)O)N1C=2N=CN=C(C=2N=C1)N)OP(O)(=O)OP(O)(=O)O[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O WFPZSXYXPSUOPY-ROYWQJLOSA-N 0.000 description 1
- 101150001232 ALS gene Proteins 0.000 description 1
- 108010003902 Acetyl-CoA C-acyltransferase Proteins 0.000 description 1
- 241001523383 Achnatherum Species 0.000 description 1
- 241000047962 Acroceras Species 0.000 description 1
- 241000186361 Actinobacteria <class> Species 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 241000209758 Aegilops Species 0.000 description 1
- 241000209136 Agropyron Species 0.000 description 1
- 241000743339 Agrostis Species 0.000 description 1
- 241000772991 Aira Species 0.000 description 1
- 108010021809 Alcohol dehydrogenase Proteins 0.000 description 1
- 108010025188 Alcohol oxidase Proteins 0.000 description 1
- 241000756998 Alismatales Species 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 235000002732 Allium cepa var. cepa Nutrition 0.000 description 1
- 241000051981 Allolepis Species 0.000 description 1
- 241001018099 Alloteropsis Species 0.000 description 1
- 241000743985 Alopecurus Species 0.000 description 1
- 241000219317 Amaranthaceae Species 0.000 description 1
- 241000209130 Amblyopyrum Species 0.000 description 1
- 241000368670 Ammophila <wasp> Species 0.000 description 1
- 241000923583 Ampelodesmos Species 0.000 description 1
- 241000070750 Amphicarpum Species 0.000 description 1
- 244000144725 Amygdalus communis Species 0.000 description 1
- 235000011437 Amygdalus communis Nutrition 0.000 description 1
- 244000144730 Amygdalus persica Species 0.000 description 1
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 241000260674 Anthaenantia Species 0.000 description 1
- 241001400082 Anthephora Species 0.000 description 1
- 241000743857 Anthoxanthum Species 0.000 description 1
- 241000207875 Antirrhinum Species 0.000 description 1
- 241001666377 Apera Species 0.000 description 1
- 240000007087 Apium graveolens Species 0.000 description 1
- 235000015849 Apium graveolens Dulce Group Nutrition 0.000 description 1
- 241000306749 Apluda Species 0.000 description 1
- 235000010591 Appio Nutrition 0.000 description 1
- 101100433502 Arabidopsis thaliana ACS7 gene Proteins 0.000 description 1
- 101100382386 Arabidopsis thaliana PPC3 gene Proteins 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 241000316756 Arctophila Species 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 241001520919 Aristida Species 0.000 description 1
- 241000508787 Arrhenatherum Species 0.000 description 1
- 241000772998 Arthraxon Species 0.000 description 1
- 241000772989 Arthrostylidium Species 0.000 description 1
- 241000209134 Arundinaria Species 0.000 description 1
- 241000931363 Arundinella Species 0.000 description 1
- 241001494510 Arundo Species 0.000 description 1
- DCXYFEDJOCDNAF-UHFFFAOYSA-N Asparagine Natural products OC(=O)C(N)CC(N)=O DCXYFEDJOCDNAF-UHFFFAOYSA-N 0.000 description 1
- 235000005340 Asparagus officinalis Nutrition 0.000 description 1
- 241001106067 Atropa Species 0.000 description 1
- 241000047982 Axonopus Species 0.000 description 1
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 1
- 241000193388 Bacillus thuringiensis Species 0.000 description 1
- 241000209128 Bambusa Species 0.000 description 1
- 241001447692 Beckmannia Species 0.000 description 1
- 241000051984 Blepharidachne Species 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 241001018635 Borinda Species 0.000 description 1
- 241001327393 Bothriochloa Species 0.000 description 1
- 241000232312 Bouteloua Species 0.000 description 1
- 241001135655 Bouteloua erecta Species 0.000 description 1
- 241000611157 Brachiaria Species 0.000 description 1
- 241000339490 Brachyachne Species 0.000 description 1
- 241001520110 Brachyelytrum Species 0.000 description 1
- 241000589174 Bradyrhizobium japonicum Species 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000011299 Brassica oleracea var botrytis Nutrition 0.000 description 1
- 235000017647 Brassica oleracea var italica Nutrition 0.000 description 1
- 240000003259 Brassica oleracea var. botrytis Species 0.000 description 1
- 241000743778 Briza Species 0.000 description 1
- 241000234670 Bromeliaceae Species 0.000 description 1
- 241000320719 Buchloe Species 0.000 description 1
- 241000743799 Calamagrostis Species 0.000 description 1
- 241000743801 Calamovilfa Species 0.000 description 1
- 101100507655 Canis lupus familiaris HSPA1 gene Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 108090000489 Carboxy-Lyases Proteins 0.000 description 1
- 235000009025 Carya illinoensis Nutrition 0.000 description 1
- 244000068645 Carya illinoensis Species 0.000 description 1
- 241001490227 Catabrosa Species 0.000 description 1
- 241000209120 Cenchrus Species 0.000 description 1
- 241000931368 Centotheca Species 0.000 description 1
- 241001520886 Chasmanthium Species 0.000 description 1
- 241001520833 Chimonobambusa Species 0.000 description 1
- 241000593460 Chionochloa Species 0.000 description 1
- 241000861718 Chloris <Aves> Species 0.000 description 1
- 239000005496 Chlorsulfuron Substances 0.000 description 1
- 241001494513 Chusquea Species 0.000 description 1
- 241000223782 Ciliophora Species 0.000 description 1
- 241000744472 Cinna Species 0.000 description 1
- 108010061190 Cinnamyl-alcohol dehydrogenase Proteins 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 241000408178 Cladoraphis Species 0.000 description 1
- 241000306777 Coelorachis Species 0.000 description 1
- 240000007154 Coffea arabica Species 0.000 description 1
- 241000209205 Coix Species 0.000 description 1
- 241000507380 Coleanthus Species 0.000 description 1
- 241000019460 Colpodium Species 0.000 description 1
- 108091035707 Consensus sequence Proteins 0.000 description 1
- 241000217270 Cornucopiae Species 0.000 description 1
- 241000593305 Cortaderia Species 0.000 description 1
- 240000009226 Corylus americana Species 0.000 description 1
- 235000001543 Corylus americana Nutrition 0.000 description 1
- 235000007466 Corylus avellana Nutrition 0.000 description 1
- 241000560213 Corynephorus Species 0.000 description 1
- 241000320752 Cottea Species 0.000 description 1
- 241001523387 Critesion Species 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- 241001018651 Crypsis Species 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000052019 Ctenium Species 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 235000010071 Cucumis prophetarum Nutrition 0.000 description 1
- 244000024469 Cucumis prophetarum Species 0.000 description 1
- 241000219122 Cucurbita Species 0.000 description 1
- 241001144051 Cutandia Species 0.000 description 1
- 241000931332 Cymbopogon Species 0.000 description 1
- FEPOUSPSESUQPD-UHFFFAOYSA-N Cymbopogon Natural products C1CC2(C)C(C)C(=O)CCC2C2(C)C1C1(C)CCC3(C)CCC(C)C(C)C3C1(C)CC2 FEPOUSPSESUQPD-UHFFFAOYSA-N 0.000 description 1
- 244000052363 Cynodon dactylon Species 0.000 description 1
- 241001551134 Cynosurus Species 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 101710096830 DNA-3-methyladenine glycosylase Proteins 0.000 description 1
- 102100039128 DNA-3-methyladenine glycosylase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 241000209210 Dactylis Species 0.000 description 1
- 241000320605 Dactyloctenium Species 0.000 description 1
- 241000052043 Dasyochloa Species 0.000 description 1
- 241000208296 Datura Species 0.000 description 1
- 241000208175 Daucus Species 0.000 description 1
- 235000013262 Dendrocalamus Nutrition 0.000 description 1
- 241000744361 Dendrocalamus Species 0.000 description 1
- 102000016911 Deoxyribonucleases Human genes 0.000 description 1
- 108010053770 Deoxyribonucleases Proteins 0.000 description 1
- 241000848289 Desmazeria Species 0.000 description 1
- 241001163087 Deyeuxia Species 0.000 description 1
- 241001520003 Diarrhena Species 0.000 description 1
- 241000338062 Dichanthelium Species 0.000 description 1
- 241001327308 Dichanthium Species 0.000 description 1
- 241001163054 Dichelachne Species 0.000 description 1
- 240000001879 Digitalis lutea Species 0.000 description 1
- 235000017896 Digitaria Nutrition 0.000 description 1
- 241001433320 Dimeria Species 0.000 description 1
- 241000145742 Dinebra Species 0.000 description 1
- 108090000204 Dipeptidase 1 Proteins 0.000 description 1
- 241000320596 Diplachne Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 241001490199 Dissanthelium Species 0.000 description 1
- 241000070810 Dissochondrus Species 0.000 description 1
- 241001306223 Drepanostachyum Species 0.000 description 1
- 241000316785 Dupontia Species 0.000 description 1
- 241000192043 Echinochloa Species 0.000 description 1
- 241000209215 Eleusine Species 0.000 description 1
- 235000007351 Eleusine Nutrition 0.000 description 1
- 241000142841 Enneapogon Species 0.000 description 1
- 101710198510 Enoyl-[acyl-carrier-protein] reductase [NADH] Proteins 0.000 description 1
- 241000145748 Enteropogon <grass> Species 0.000 description 1
- 241001518935 Eragrostis Species 0.000 description 1
- 241001433310 Eremochloa Species 0.000 description 1
- 241001490176 Eremopoa Species 0.000 description 1
- 241001523362 Eremopyrum Species 0.000 description 1
- 241000606889 Eriochrysis Species 0.000 description 1
- 241000052079 Erioneuron Species 0.000 description 1
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 1
- 241001585085 Euchlaena Species 0.000 description 1
- 241001606303 Euclasta <moth> Species 0.000 description 1
- 241000945868 Eulaliopsis Species 0.000 description 1
- 241001520106 Eustachys Species 0.000 description 1
- 102000010834 Extracellular Matrix Proteins Human genes 0.000 description 1
- 108010037362 Extracellular Matrix Proteins Proteins 0.000 description 1
- 241000161991 Fargesia Species 0.000 description 1
- 108010087894 Fatty acid desaturases Proteins 0.000 description 1
- 241000032244 Festuca subg. Vulpia Species 0.000 description 1
- 241000145757 Fingerhuthia Species 0.000 description 1
- 241000220223 Fragaria Species 0.000 description 1
- 230000005526 G1 to G0 transition Effects 0.000 description 1
- 241001414529 Garnotia Species 0.000 description 1
- 241001476001 Gastridium <grass> Species 0.000 description 1
- 241000560209 Gaudinia Species 0.000 description 1
- 241000208152 Geranium Species 0.000 description 1
- 235000003508 Gigantochloa thoii Nutrition 0.000 description 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 1
- 235000000512 Glyceria Nutrition 0.000 description 1
- 239000004471 Glycine Substances 0.000 description 1
- 241001476003 Graphephorum Species 0.000 description 1
- 241000320721 Gymnopogon Species 0.000 description 1
- 241000142762 Gynerium Species 0.000 description 1
- 241000744834 Hakonechloa Species 0.000 description 1
- 241000122202 Haynaldia Species 0.000 description 1
- 241000208818 Helianthus Species 0.000 description 1
- 241000245622 Helictotrichon Species 0.000 description 1
- 241000931313 Hemarthria Species 0.000 description 1
- 241000270901 Hesperostipa Species 0.000 description 1
- 241001582739 Heteropogon <robber fly> Species 0.000 description 1
- 241000773457 Hibanobambusa Species 0.000 description 1
- 241000825107 Hierochloe Species 0.000 description 1
- 241000052086 Hilaria Species 0.000 description 1
- 108010033040 Histones Proteins 0.000 description 1
- 241000744855 Holcus Species 0.000 description 1
- 101000780205 Homo sapiens Long-chain-fatty-acid-CoA ligase 5 Proteins 0.000 description 1
- 101000780202 Homo sapiens Long-chain-fatty-acid-CoA ligase 6 Proteins 0.000 description 1
- 101000582320 Homo sapiens Neurogenic differentiation factor 6 Proteins 0.000 description 1
- 108010001336 Horseradish Peroxidase Proteins 0.000 description 1
- 241000044414 Hymenachne Species 0.000 description 1
- 241000208278 Hyoscyamus Species 0.000 description 1
- 244000265274 Hyparrhenia filipendula Species 0.000 description 1
- 241000044417 Ichnanthus Species 0.000 description 1
- 240000007171 Imperata cylindrica Species 0.000 description 1
- 241000755540 Indocalamus Species 0.000 description 1
- 229930010555 Inosine Natural products 0.000 description 1
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 240000006392 Isachne Species 0.000 description 1
- 241001327265 Ischaemum Species 0.000 description 1
- 241000496998 Ixophorus Species 0.000 description 1
- 241000758789 Juglans Species 0.000 description 1
- 235000013757 Juglans Nutrition 0.000 description 1
- 240000007049 Juglans regia Species 0.000 description 1
- 235000009496 Juglans regia Nutrition 0.000 description 1
- 108010025815 Kanamycin Kinase Proteins 0.000 description 1
- 241000191948 Kocuria rosea Species 0.000 description 1
- 241000245643 Koeleria Species 0.000 description 1
- 241000235058 Komagataella pastoris Species 0.000 description 1
- DCXYFEDJOCDNAF-REOHCLBHSA-N L-asparagine Chemical compound OC(=O)[C@@H](N)CC(N)=O DCXYFEDJOCDNAF-REOHCLBHSA-N 0.000 description 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 description 1
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 description 1
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 description 1
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 1
- 229930182821 L-proline Natural products 0.000 description 1
- OUYCCCASQSFEME-QMMMGPOBSA-N L-tyrosine Chemical compound OC(=O)[C@@H](N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-QMMMGPOBSA-N 0.000 description 1
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 241000208822 Lactuca Species 0.000 description 1
- 241001444143 Lagurus <angiosperm> Species 0.000 description 1
- 241000744787 Lamarckia Species 0.000 description 1
- 241000044443 Lasiacis Species 0.000 description 1
- 240000006568 Lathyrus odoratus Species 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 241001494496 Leersia Species 0.000 description 1
- 235000014647 Lens culinaris subsp culinaris Nutrition 0.000 description 1
- 244000043158 Lens esculenta Species 0.000 description 1
- 241000320639 Leptochloa Species 0.000 description 1
- 241000145637 Lepturus Species 0.000 description 1
- ROHFNLRQFUQHCH-UHFFFAOYSA-N Leucine Natural products CC(C)CC(N)C(O)=O ROHFNLRQFUQHCH-UHFFFAOYSA-N 0.000 description 1
- 241000511731 Leymus Species 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 241000234269 Liliales Species 0.000 description 1
- 241001497103 Limnodea Species 0.000 description 1
- 241000208204 Linum Species 0.000 description 1
- 241001494498 Lithachne Species 0.000 description 1
- 102100034318 Long-chain-fatty-acid-CoA ligase 5 Human genes 0.000 description 1
- 241001421529 Lophopyrum Species 0.000 description 1
- 241000219745 Lupinus Species 0.000 description 1
- 241001484124 Luziola Species 0.000 description 1
- 235000002262 Lycopersicon Nutrition 0.000 description 1
- 241000745397 Lygeum Species 0.000 description 1
- 241000121629 Majorana Species 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 241001507457 Manisuris Species 0.000 description 1
- 241001329086 Megastachya Species 0.000 description 1
- 241000213996 Melilotus Species 0.000 description 1
- 235000000839 Melilotus officinalis subsp suaveolens Nutrition 0.000 description 1
- 101100409013 Mesembryanthemum crystallinum PPD gene Proteins 0.000 description 1
- 241000560223 Mibora Species 0.000 description 1
- 241000526657 Microchloa Species 0.000 description 1
- 241000306779 Microstegium Species 0.000 description 1
- 241001490213 Milium Species 0.000 description 1
- 240000003433 Miscanthus floridulus Species 0.000 description 1
- 241000878006 Miscanthus sinensis Species 0.000 description 1
- 241001401380 Mnesithea Species 0.000 description 1
- 229920000881 Modified starch Polymers 0.000 description 1
- 241001520029 Molinia Species 0.000 description 1
- 244000111261 Mucuna pruriens Species 0.000 description 1
- 235000008540 Mucuna pruriens var utilis Nutrition 0.000 description 1
- 241000592161 Muhlenbergia Species 0.000 description 1
- 241000052145 Munroa Species 0.000 description 1
- 240000005561 Musa balbisiana Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 241000204025 Mycoplasma capricolum Species 0.000 description 1
- 229910018890 NaMoO4 Inorganic materials 0.000 description 1
- 241000745334 Nardus Species 0.000 description 1
- 241001230286 Narenga Species 0.000 description 1
- 241001523369 Nassella Species 0.000 description 1
- 241001282315 Nemesis Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241000495186 Neoschischkinia Species 0.000 description 1
- 241000320703 Neostapfia Species 0.000 description 1
- 102100030589 Neurogenic differentiation factor 6 Human genes 0.000 description 1
- 241001018646 Neyraudia Species 0.000 description 1
- 241000208125 Nicotiana Species 0.000 description 1
- 241000208133 Nicotiana plumbaginifolia Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- GRYLNZFGIOXLOG-UHFFFAOYSA-N Nitric acid Chemical compound O[N+]([O-])=O GRYLNZFGIOXLOG-UHFFFAOYSA-N 0.000 description 1
- 239000000020 Nitrocellulose Substances 0.000 description 1
- 108091092724 Noncoding DNA Proteins 0.000 description 1
- 108091005461 Nucleic proteins Proteins 0.000 description 1
- 241001520828 Olyra <angiosperm> Species 0.000 description 1
- 241000219830 Onobrychis Species 0.000 description 1
- 241000931244 Oplismenus Species 0.000 description 1
- 241000145654 Orcuttia Species 0.000 description 1
- 241001518636 Oryzopsis Species 0.000 description 1
- 241000216616 Otatea Species 0.000 description 1
- 241001645266 Oxytenanthera Species 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 235000001591 Pachyrhizus erosus Nutrition 0.000 description 1
- 244000258470 Pachyrhizus tuberosus Species 0.000 description 1
- 235000018669 Pachyrhizus tuberosus Nutrition 0.000 description 1
- 241000209117 Panicum Species 0.000 description 1
- 235000006443 Panicum miliaceum subsp. miliaceum Nutrition 0.000 description 1
- 235000009037 Panicum miliaceum subsp. ruderale Nutrition 0.000 description 1
- 241000145659 Pappophorum Species 0.000 description 1
- 241001550949 Parapholis Species 0.000 description 1
- 206010034016 Paronychia Diseases 0.000 description 1
- 241001668545 Pascopyrum Species 0.000 description 1
- 241000044524 Paspalidium Species 0.000 description 1
- 241001330453 Paspalum Species 0.000 description 1
- 241000208181 Pelargonium Species 0.000 description 1
- 108010087702 Penicillinase Proteins 0.000 description 1
- 108091093037 Peptide nucleic acid Proteins 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 241000745991 Phalaris Species 0.000 description 1
- 241000226265 Phanopyrum Species 0.000 description 1
- 241000829202 Pharus <Mollusca> Species 0.000 description 1
- 241000219833 Phaseolus Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 101000870887 Phaseolus vulgaris Glycine-rich cell wall structural protein 1.8 Proteins 0.000 description 1
- 241001490218 Phippsia Species 0.000 description 1
- 241000746981 Phleum Species 0.000 description 1
- 241001401260 Pholiurus Species 0.000 description 1
- 102000011755 Phosphoglycerate Kinase Human genes 0.000 description 1
- 108091000080 Phosphotransferase Proteins 0.000 description 1
- 235000014676 Phragmites communis Nutrition 0.000 description 1
- 244000273256 Phragmites communis Species 0.000 description 1
- 241000745988 Phyllostachys Species 0.000 description 1
- 241000235648 Pichia Species 0.000 description 1
- 235000005205 Pinus Nutrition 0.000 description 1
- 241000218602 Pinus <genus> Species 0.000 description 1
- 241000508729 Piptatherum Species 0.000 description 1
- 241001523341 Piptochaetium Species 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 241000400666 Pleioblastus Species 0.000 description 1
- 241000145176 Pleuraphis Species 0.000 description 1
- 241000635397 Pleuropogon Species 0.000 description 1
- 241000209048 Poa Species 0.000 description 1
- 241000209504 Poaceae Species 0.000 description 1
- 241000848268 Podagrostis Species 0.000 description 1
- 239000002202 Polyethylene glycol Substances 0.000 description 1
- 229920000331 Polyhydroxybutyrate Polymers 0.000 description 1
- 241000724416 Polypogon <angiosperm> Species 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 241001433241 Polytrias Species 0.000 description 1
- 239000004372 Polyvinyl alcohol Substances 0.000 description 1
- 241000219000 Populus Species 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 235000009827 Prunus armeniaca Nutrition 0.000 description 1
- 244000018633 Prunus armeniaca Species 0.000 description 1
- 235000006040 Prunus persica var persica Nutrition 0.000 description 1
- 241000209122 Psathyrostachys Species 0.000 description 1
- 241000216609 Pseudosasa Species 0.000 description 1
- 241000270678 Ptilagrostis Species 0.000 description 1
- 241000736229 Puccinellia Species 0.000 description 1
- 108010066717 Q beta Replicase Proteins 0.000 description 1
- 241000219492 Quercus Species 0.000 description 1
- 108020005161 RNA Caps Proteins 0.000 description 1
- 108020005067 RNA Splice Sites Proteins 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 101150075111 ROLB gene Proteins 0.000 description 1
- 241000218206 Ranunculus Species 0.000 description 1
- 241000220259 Raphanus Species 0.000 description 1
- 108020004511 Recombinant DNA Proteins 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 108700005075 Regulator Genes Proteins 0.000 description 1
- 241000073276 Reimarochloa Species 0.000 description 1
- 241000589180 Rhizobium Species 0.000 description 1
- 235000011449 Rosa Nutrition 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241000857233 Rottboellia Species 0.000 description 1
- 240000007651 Rubus glaucus Species 0.000 description 1
- 235000011034 Rubus glaucus Nutrition 0.000 description 1
- 235000009122 Rubus idaeus Nutrition 0.000 description 1
- 108091006629 SLC13A2 Proteins 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 241000209051 Saccharum Species 0.000 description 1
- 241000044558 Sacciolepis Species 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241001106018 Salpiglossis Species 0.000 description 1
- 240000005499 Sasa Species 0.000 description 1
- 241000267039 Sasaella Species 0.000 description 1
- 241000611425 Schismus Species 0.000 description 1
- 241000147395 Schizachne Species 0.000 description 1
- 241001327272 Schizachyrium Species 0.000 description 1
- 241000216509 Schizostachyum Species 0.000 description 1
- 241001550934 Sclerochloa Species 0.000 description 1
- 241000052179 Scleropogon <grass> Species 0.000 description 1
- 241000158927 Scolochloa Species 0.000 description 1
- 241000319026 Scribneria Species 0.000 description 1
- 241000543808 Semiarundinaria Species 0.000 description 1
- 241000780602 Senecio Species 0.000 description 1
- 241001116459 Sequoia Species 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 241000508743 Sesleria Species 0.000 description 1
- 235000005775 Setaria Nutrition 0.000 description 1
- 241000232088 Setaria <nematode> Species 0.000 description 1
- 241000498786 Shibataea Species 0.000 description 1
- 241000220261 Sinapis Species 0.000 description 1
- 241000543826 Sinobambusa Species 0.000 description 1
- 108010052160 Site-specific recombinase Proteins 0.000 description 1
- 235000002634 Solanum Nutrition 0.000 description 1
- 241000207763 Solanum Species 0.000 description 1
- 101000915835 Solanum lycopersicum 1-aminocyclopropane-1-carboxylate synthase 3 Proteins 0.000 description 1
- 241001327268 Sorghastrum Species 0.000 description 1
- 101000815956 Sorghum bicolor DNA-directed RNA polymerase subunit alpha Proteins 0.000 description 1
- 101000727343 Sorghum bicolor DNA-directed RNA polymerase subunit beta Proteins 0.000 description 1
- 101001088501 Sorghum bicolor DNA-directed RNA polymerase subunit beta' Proteins 0.000 description 1
- 101000649260 Sorghum bicolor DNA-directed RNA polymerase subunit beta'' Proteins 0.000 description 1
- 241000746413 Spartina Species 0.000 description 1
- 241000560237 Sphenopholis Species 0.000 description 1
- 241001140642 Spodiopogon Species 0.000 description 1
- 241001520881 Sporobolus Species 0.000 description 1
- 101100289792 Squirrel monkey polyomavirus large T gene Proteins 0.000 description 1
- 108010039811 Starch synthase Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 241000044574 Steinchisma Species 0.000 description 1
- 244000087212 Stenotaphrum Species 0.000 description 1
- 241000746422 Stipa Species 0.000 description 1
- 241000142724 Stipagrostis Species 0.000 description 1
- 238000000692 Student's t-test Methods 0.000 description 1
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000005864 Sulphur Substances 0.000 description 1
- 241001523334 Taeniatherum Species 0.000 description 1
- 239000004098 Tetracycline Substances 0.000 description 1
- 241001306213 Thamnocalamus Species 0.000 description 1
- 241000931197 Themeda Species 0.000 description 1
- 101001099217 Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) Triosephosphate isomerase Proteins 0.000 description 1
- 101710194092 Thiamine-phosphate synthase 1 Proteins 0.000 description 1
- 241000209124 Thinopyrum Species 0.000 description 1
- 102000002933 Thioredoxin Human genes 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 241001208278 Thuarea Species 0.000 description 1
- 241001520811 Thysanolaena Species 0.000 description 1
- 241000848292 Torreyochloa Species 0.000 description 1
- 241000592342 Tracheophyta Species 0.000 description 1
- 241001528284 Trachypogon Species 0.000 description 1
- 241000746998 Tragus Species 0.000 description 1
- 241000145178 Trichloris Species 0.000 description 1
- 241000746999 Tricholaena Species 0.000 description 1
- 241000145171 Trichoneura Species 0.000 description 1
- 241000145122 Tridens <angiosperm> Species 0.000 description 1
- 241001312519 Trigonella Species 0.000 description 1
- 241000931213 Triodia <angiosperm> Species 0.000 description 1
- 241000052209 Triplasis Species 0.000 description 1
- 241000052213 Tripogon Species 0.000 description 1
- 241000209138 Tripsacum Species 0.000 description 1
- 239000007984 Tris EDTA buffer Substances 0.000 description 1
- 241000005601 Trisetum Species 0.000 description 1
- 235000019714 Triticale Nutrition 0.000 description 1
- 241000320679 Tuctoria Species 0.000 description 1
- 241000145124 Uniola Species 0.000 description 1
- 241001521331 Urochloa Species 0.000 description 1
- 241000372906 Vahlodea Species 0.000 description 1
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 1
- 241000320760 Vaseyochloa Species 0.000 description 1
- 241000172367 Ventenata Species 0.000 description 1
- 241000414043 Vetiveria Species 0.000 description 1
- 241000219873 Vicia Species 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 235000009392 Vitis Nutrition 0.000 description 1
- 241000219095 Vitis Species 0.000 description 1
- 241000052229 Willkommia Species 0.000 description 1
- 241000219995 Wisteria Species 0.000 description 1
- 241001523432 Zale Species 0.000 description 1
- 241000209149 Zea Species 0.000 description 1
- 241000746966 Zizania Species 0.000 description 1
- 241001478413 Zizaniopsis Species 0.000 description 1
- 240000001102 Zoysia matrella Species 0.000 description 1
- FHHZHGZBHYYWTG-INFSMZHSSA-N [(2r,3s,4r,5r)-5-(2-amino-7-methyl-6-oxo-3h-purin-9-ium-9-yl)-3,4-dihydroxyoxolan-2-yl]methyl [[[(2r,3s,4r,5r)-5-(2-amino-6-oxo-3h-purin-9-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-hydroxyphosphoryl] phosphate Chemical compound N1C(N)=NC(=O)C2=C1[N+]([C@H]1[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=C(C(N=C(N)N4)=O)N=C3)O)O1)O)=CN2C FHHZHGZBHYYWTG-INFSMZHSSA-N 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 230000006578 abscission Effects 0.000 description 1
- 108091000039 acetoacetyl-CoA reductase Proteins 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 244000193174 agave Species 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- 102000004139 alpha-Amylases Human genes 0.000 description 1
- 108090000637 alpha-Amylases Proteins 0.000 description 1
- 229940024171 alpha-amylase Drugs 0.000 description 1
- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 210000004102 animal cell Anatomy 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 239000003242 anti bacterial agent Substances 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 229940072107 ascorbate Drugs 0.000 description 1
- 235000010323 ascorbic acid Nutrition 0.000 description 1
- 239000011668 ascorbic acid Substances 0.000 description 1
- 229960001230 asparagine Drugs 0.000 description 1
- 235000009582 asparagine Nutrition 0.000 description 1
- 235000003704 aspartic acid Nutrition 0.000 description 1
- 230000000680 avirulence Effects 0.000 description 1
- CSGLCWIAEFNDIL-UHFFFAOYSA-O azanium;urea;nitrate Chemical compound [NH4+].NC(N)=O.[O-][N+]([O-])=O CSGLCWIAEFNDIL-UHFFFAOYSA-O 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 230000010310 bacterial transformation Effects 0.000 description 1
- 108010021384 barley lectin Proteins 0.000 description 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 description 1
- 102000006635 beta-lactamase Human genes 0.000 description 1
- 229910002056 binary alloy Inorganic materials 0.000 description 1
- 239000003139 biocide Substances 0.000 description 1
- 229920000704 biodegradable plastic Polymers 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- HOZOZZFCZRXYEK-GSWUYBTGSA-M butylscopolamine bromide Chemical compound [Br-].C1([C@@H](CO)C(=O)O[C@H]2C[C@@H]3[N+]([C@H](C2)[C@@H]2[C@H]3O2)(C)CCCC)=CC=CC=C1 HOZOZZFCZRXYEK-GSWUYBTGSA-M 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 101150039352 can gene Proteins 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 239000006143 cell culture medium Substances 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 108010040093 cellulose synthase Proteins 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- VJYIFXVZLXQVHO-UHFFFAOYSA-N chlorsulfuron Chemical compound COC1=NC(C)=NC(NC(=O)NS(=O)(=O)C=2C(=CC=CC=2)Cl)=N1 VJYIFXVZLXQVHO-UHFFFAOYSA-N 0.000 description 1
- 230000002759 chromosomal effect Effects 0.000 description 1
- 108010069282 cis-prenyl transferase Proteins 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 229910001429 cobalt ion Inorganic materials 0.000 description 1
- XLJKHNWPARRRJB-UHFFFAOYSA-N cobalt(2+) Chemical compound [Co+2] XLJKHNWPARRRJB-UHFFFAOYSA-N 0.000 description 1
- 235000016213 coffee Nutrition 0.000 description 1
- 235000013353 coffee beverage Nutrition 0.000 description 1
- 230000008645 cold stress Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000004883 computer application Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 125000004122 cyclic group Chemical group 0.000 description 1
- 239000004062 cytokinin Substances 0.000 description 1
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 description 1
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical class NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 1
- 210000000172 cytosol Anatomy 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000005547 deoxyribonucleotide Substances 0.000 description 1
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000005059 dormancy Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000005489 dwarf bean Nutrition 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 108010065744 ethylene forming enzyme Proteins 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 230000007717 exclusion Effects 0.000 description 1
- 238000013401 experimental design Methods 0.000 description 1
- 210000002744 extracellular matrix Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 239000012458 free base Substances 0.000 description 1
- 230000004345 fruit ripening Effects 0.000 description 1
- 239000003008 fumonisin Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000012239 gene modification Methods 0.000 description 1
- 238000010363 gene targeting Methods 0.000 description 1
- 235000013922 glutamic acid Nutrition 0.000 description 1
- 239000004220 glutamic acid Substances 0.000 description 1
- 208000037824 growth disorder Diseases 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 230000008642 heat stress Effects 0.000 description 1
- 230000006801 homologous recombination Effects 0.000 description 1
- 238000002744 homologous recombination Methods 0.000 description 1
- 229920001519 homopolymer Polymers 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 238000003365 immunocytochemistry Methods 0.000 description 1
- 230000003116 impacting effect Effects 0.000 description 1
- 238000007901 in situ hybridization Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000009399 inbreeding Methods 0.000 description 1
- 239000003617 indole-3-acetic acid Substances 0.000 description 1
- 229960003786 inosine Drugs 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 230000016507 interphase Effects 0.000 description 1
- 229960000310 isoleucine Drugs 0.000 description 1
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 description 1
- 229960000318 kanamycin Drugs 0.000 description 1
- 229930027917 kanamycin Natural products 0.000 description 1
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 1
- 229930182823 kanamycin A Natural products 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 230000011890 leaf development Effects 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000002502 liposome Substances 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 108010026228 mRNA guanylyltransferase Proteins 0.000 description 1
- 210000002231 macronucleus Anatomy 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 210000001161 mammalian embryo Anatomy 0.000 description 1
- 235000005739 manihot Nutrition 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 230000020429 meristem development Effects 0.000 description 1
- 230000000442 meristematic effect Effects 0.000 description 1
- 210000000473 mesophyll cell Anatomy 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 235000019426 modified starch Nutrition 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 230000000869 mutational effect Effects 0.000 description 1
- 230000001338 necrotic effect Effects 0.000 description 1
- 229910017604 nitric acid Inorganic materials 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 230000031787 nutrient reservoir activity Effects 0.000 description 1
- 235000014571 nuts Nutrition 0.000 description 1
- 230000005305 organ development Effects 0.000 description 1
- 238000009401 outcrossing Methods 0.000 description 1
- 239000005022 packaging material Substances 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 229950009506 penicillinase Drugs 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 1
- 150000008300 phosphoramidites Chemical class 0.000 description 1
- 102000020233 phosphotransferase Human genes 0.000 description 1
- 238000000596 photon cross correlation spectroscopy Methods 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229930195732 phytohormone Natural products 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000003375 plant hormone Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 230000008119 pollen development Effects 0.000 description 1
- 239000005015 poly(hydroxybutyrate) Substances 0.000 description 1
- 229920003223 poly(pyromellitimide-1,4-diphenyl ether) Polymers 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 229920001223 polyethylene glycol Polymers 0.000 description 1
- 238000006116 polymerization reaction Methods 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 108010055896 polyornithine Proteins 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920002451 polyvinyl alcohol Polymers 0.000 description 1
- 230000001124 posttranscriptional effect Effects 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 101150063097 ppdK gene Proteins 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 235000007682 pyridoxal 5'-phosphate Nutrition 0.000 description 1
- 239000011589 pyridoxal 5'-phosphate Substances 0.000 description 1
- 229960001327 pyridoxal phosphate Drugs 0.000 description 1
- NHDHVHZZCFYRSB-UHFFFAOYSA-N pyriproxyfen Chemical compound C=1C=CC=NC=1OC(C)COC(C=C1)=CC=C1OC1=CC=CC=C1 NHDHVHZZCFYRSB-UHFFFAOYSA-N 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 238000009790 rate-determining step (RDS) Methods 0.000 description 1
- 238000012755 real-time RT-PCR analysis Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000000306 recurrent effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 230000022983 regulation of cell cycle Effects 0.000 description 1
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000007894 restriction fragment length polymorphism technique Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000005070 ripening Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 230000011869 shoot development Effects 0.000 description 1
- HBMJWWWQQXIZIP-UHFFFAOYSA-N silicon carbide Chemical compound [Si+]#[C-] HBMJWWWQQXIZIP-UHFFFAOYSA-N 0.000 description 1
- 229910010271 silicon carbide Inorganic materials 0.000 description 1
- 235000017550 sodium carbonate Nutrition 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 244000000000 soil microbiome Species 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 229960000268 spectinomycin Drugs 0.000 description 1
- UNFWWIHTNXNPBV-WXKVUWSESA-N spectinomycin Chemical compound O([C@@H]1[C@@H](NC)[C@@H](O)[C@H]([C@@H]([C@H]1O1)O)NC)[C@]2(O)[C@H]1O[C@H](C)CC2=O UNFWWIHTNXNPBV-WXKVUWSESA-N 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 230000000707 stereoselective effect Effects 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000011550 stock solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 238000013517 stratification Methods 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 235000011149 sulphuric acid Nutrition 0.000 description 1
- 230000000153 supplemental effect Effects 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 238000010189 synthetic method Methods 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 229960002180 tetracycline Drugs 0.000 description 1
- 229930101283 tetracycline Natural products 0.000 description 1
- 235000019364 tetracycline Nutrition 0.000 description 1
- 150000003522 tetracyclines Chemical class 0.000 description 1
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 description 1
- 229940033663 thimerosal Drugs 0.000 description 1
- 108060008226 thioredoxin Proteins 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- 230000025366 tissue development Effects 0.000 description 1
- 230000006032 tissue transformation Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 238000003151 transfection method Methods 0.000 description 1
- 238000000844 transformation Methods 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- 230000005068 transpiration Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 239000013638 trimer Substances 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- OUYCCCASQSFEME-UHFFFAOYSA-N tyrosine Natural products OC(=O)C(N)CC1=CC=C(O)C=C1 OUYCCCASQSFEME-UHFFFAOYSA-N 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
- 239000004474 valine Substances 0.000 description 1
- 210000005167 vascular cell Anatomy 0.000 description 1
- 210000005166 vasculature Anatomy 0.000 description 1
- 108700026220 vif Genes Proteins 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 238000011179 visual inspection Methods 0.000 description 1
- 235000020234 walnut Nutrition 0.000 description 1
- 244000034968 watho Species 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 241000110300 x Agropogon Species 0.000 description 1
- 241000441614 x Festulolium Species 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
- 235000009529 zinc sulphate Nutrition 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
- C12N15/8249—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving ethylene biosynthesis, senescence or fruit development, e.g. modified tomato ripening, cut flower shelf-life
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8273—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for drought, cold, salt resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Applications Claiming Priority (7)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US24806009P | 2009-10-02 | 2009-10-02 | |
| US61/248,060 | 2009-10-02 | ||
| US29090209P | 2009-12-30 | 2009-12-30 | |
| US61/290,902 | 2009-12-30 | ||
| US33206910P | 2010-05-06 | 2010-05-06 | |
| US61/332,069 | 2010-05-06 | ||
| PCT/US2010/051358 WO2011041796A1 (en) | 2009-10-02 | 2010-10-04 | Down-regulation of acc synthase for improved plant performance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2775093A1 true CA2775093A1 (en) | 2011-04-07 |
Family
ID=43566754
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2775093A Abandoned CA2775093A1 (en) | 2009-10-02 | 2010-10-04 | Down-regulation of acc synthase for improved plant performance |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US20110252505A1 (enExample) |
| EP (1) | EP2483404A1 (enExample) |
| CN (1) | CN102597229A (enExample) |
| AR (1) | AR078502A1 (enExample) |
| BR (1) | BR112012007516A2 (enExample) |
| CA (1) | CA2775093A1 (enExample) |
| IN (1) | IN2012DN02408A (enExample) |
| MX (1) | MX2012003972A (enExample) |
| WO (1) | WO2011041796A1 (enExample) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| UA108071C2 (uk) * | 2009-04-14 | 2015-03-25 | Піонер Хай-Бред Інтернешнл, Інк. | Спосіб поліпшення витривалості до нестачі азоту у рослини |
| CN102732528A (zh) * | 2011-04-15 | 2012-10-17 | 华中农业大学 | Oxhs4基因在控制水稻抗旱性中的应用 |
| CN106591354B (zh) * | 2016-12-02 | 2019-08-23 | 武汉生物工程学院 | 氨基酸转运基因OsAAP5在水稻选育中的应用 |
| US10378459B2 (en) * | 2017-03-23 | 2019-08-13 | Ford Global Technologies, Llc | Method and system for engine control |
| CN110199877A (zh) * | 2019-07-04 | 2019-09-06 | 安徽省农业科学院棉花研究所 | 一种通过小孢子培养获得表型稳定的纯合薏苡资源的方法 |
Family Cites Families (89)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US542901A (en) | 1895-07-16 | Cabinet | ||
| US4658082A (en) | 1984-07-25 | 1987-04-14 | Atlantic Richfield Company | Method for producing intact plants containing foreign DNA |
| US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| ES2018274T5 (es) | 1986-03-11 | 1996-12-16 | Plant Genetic Systems Nv | Celulas vegetales resistentes a los inhibidores de glutamina sintetasa, preparadas por ingenieria genetica. |
| US5453566A (en) | 1986-03-28 | 1995-09-26 | Calgene, Inc. | Antisense regulation of gene expression in plant/cells |
| US4987071A (en) | 1986-12-03 | 1991-01-22 | University Patents, Inc. | RNA ribozyme polymerases, dephosphorylases, restriction endoribonucleases and methods |
| JPH0293088A (ja) | 1988-09-29 | 1990-04-03 | Permelec Electrode Ltd | 水電解方法及び装置 |
| US5023179A (en) | 1988-11-14 | 1991-06-11 | Eric Lam | Promoter enhancer element for gene expression in plant roots |
| US5110732A (en) | 1989-03-14 | 1992-05-05 | The Rockefeller University | Selective gene expression in plants |
| US5034323A (en) | 1989-03-30 | 1991-07-23 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
| US5231020A (en) | 1989-03-30 | 1993-07-27 | Dna Plant Technology Corporation | Genetic engineering of novel plant phenotypes |
| US5262306A (en) | 1989-09-26 | 1993-11-16 | Robeson David J | Methods for identifying cercosporin-degrading microorganisms |
| HU220773B1 (hu) | 1990-01-22 | 2002-05-28 | Dekalb Genetics Corporation | Eljárás termő transzgenikus kukoricanövények előállítására |
| AU639788B2 (en) | 1990-04-26 | 1993-08-05 | Plant Genetic Systems N.V. | New bacillus thuringiensis strains and their genes encoding insecticidal toxins |
| US5704160A (en) | 1990-11-16 | 1998-01-06 | E. I. Du Pont De Nemours And Company | Production method for high-oil corn grain |
| US5706603A (en) | 1990-11-16 | 1998-01-13 | E. I. Du Pont De Nemours And Company | Production method for corn with enhanced quality grain traits |
| US5277905A (en) | 1991-01-16 | 1994-01-11 | Mycogen Corporation | Coleopteran-active bacillus thuringiensis isolate |
| KR100241117B1 (ko) | 1991-08-02 | 2000-02-01 | 코헤이 미쯔이 | 신규한 미생물 및 살충제 |
| AU682659B2 (en) | 1991-10-04 | 1997-10-16 | North Carolina State University | Pathogen-resistant transgenic plants |
| CA2124673C (en) | 1991-12-04 | 2008-08-05 | John Browse | Fatty acid desaturase genes from plants |
| US5341001A (en) | 1992-02-13 | 1994-08-23 | Matsushita Electric Industrial Co., Ltd. | Sulfide-selenide manganese-zinc mixed crystal photo semiconductor and laser diode |
| GB9210273D0 (en) | 1992-05-13 | 1992-07-01 | Ici Plc | Dna |
| DE69334225D1 (de) | 1992-07-07 | 2008-07-31 | Japan Tobacco Inc | Verfahren zur transformation einer monokotyledon pflanze |
| WO1994011516A1 (en) | 1992-11-17 | 1994-05-26 | E.I. Du Pont De Nemours And Company | Genes for microsomal delta-12 fatty acid desaturases and related enzymes from plants |
| WO1994012014A1 (en) | 1992-11-20 | 1994-06-09 | Agracetus, Inc. | Transgenic cotton plants producing heterologous bioplastic |
| DE69428290T2 (de) | 1993-01-13 | 2002-04-18 | Pioneer Hi-Bred International, Inc. | Derivate von alpha-hordothionin mit höherem behalt an lysin |
| US5583210A (en) | 1993-03-18 | 1996-12-10 | Pioneer Hi-Bred International, Inc. | Methods and compositions for controlling plant development |
| CA2148499C (en) | 1993-09-03 | 2006-07-11 | Hideaki Saito | Method for transforming monocotyledons using scutella of immature embryos |
| US5426039A (en) | 1993-09-08 | 1995-06-20 | Bio-Rad Laboratories, Inc. | Direct molecular cloning of primer extended DNA containing an alkane diol |
| US5470353A (en) | 1993-10-20 | 1995-11-28 | Hollister Incorporated | Post-operative thermal blanket |
| KR100386337B1 (ko) | 1993-12-09 | 2004-03-24 | 토마스 제퍼슨 대학교 | 진핵세포에서부위-특이적돌연변이를위한화합물과그방법 |
| JPH07177130A (ja) | 1993-12-21 | 1995-07-14 | Fujitsu Ltd | エラーカウント回路 |
| US5559252A (en) | 1994-02-16 | 1996-09-24 | The Dupont Merck Pharmaceutical Company | Process for the preparation of diamine intermediates useful in the synthesis of HIV protease inhibitors |
| US5593881A (en) | 1994-05-06 | 1997-01-14 | Mycogen Corporation | Bacillus thuringiensis delta-endotoxin |
| US5633363A (en) | 1994-06-03 | 1997-05-27 | Iowa State University, Research Foundation In | Root preferential promoter |
| US5501836A (en) | 1994-07-11 | 1996-03-26 | Hewlett Packard Company | Entrapped non-enzymatic macromolecules for chemical sensing |
| US5736369A (en) | 1994-07-29 | 1998-04-07 | Pioneer Hi-Bred International, Inc. | Method for producing transgenic cereal plants |
| US5792931A (en) | 1994-08-12 | 1998-08-11 | Pioneer Hi-Bred International, Inc. | Fumonisin detoxification compositions and methods |
| US5659026A (en) | 1995-03-24 | 1997-08-19 | Pioneer Hi-Bred International | ALS3 promoter |
| CA2222615A1 (en) | 1995-06-02 | 1996-12-05 | Gururaj A. Rao | High threonine derivatives of .alpha.-hordothionin |
| BR9609299A (pt) | 1995-06-02 | 1999-05-11 | Pioneer Hi Bred Int | Derivados de &-hordotionina com elevado teor de metionina |
| US5837876A (en) | 1995-07-28 | 1998-11-17 | North Carolina State University | Root cortex specific gene promoter |
| US5737514A (en) | 1995-11-29 | 1998-04-07 | Texas Micro, Inc. | Remote checkpoint memory system and protocol for fault-tolerant computer system |
| US5693512A (en) | 1996-03-01 | 1997-12-02 | The Ohio State Research Foundation | Method for transforming plant tissue by sonication |
| US5731181A (en) | 1996-06-17 | 1998-03-24 | Thomas Jefferson University | Chimeric mutational vectors having non-natural nucleotides |
| US5760012A (en) | 1996-05-01 | 1998-06-02 | Thomas Jefferson University | Methods and compounds for curing diseases caused by mutations |
| AU3495297A (en) | 1996-07-08 | 1998-02-02 | Pioneer Hi-Bred International, Inc. | Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs |
| US5824875A (en) * | 1996-10-01 | 1998-10-20 | Colorado State University Through Its Agent Colorado State University Research Foundation | 1-aminocyclopropane-1-carboxylate synthase genes from pelargonium |
| JP3441899B2 (ja) | 1996-11-01 | 2003-09-02 | 理化学研究所 | 完全長ｃＤＮＡライブラリーの作成方法 |
| US6232529B1 (en) | 1996-11-20 | 2001-05-15 | Pioneer Hi-Bred International, Inc. | Methods of producing high-oil seed by modification of starch levels |
| US5981840A (en) | 1997-01-24 | 1999-11-09 | Pioneer Hi-Bred International, Inc. | Methods for agrobacterium-mediated transformation |
| GB9703146D0 (en) | 1997-02-14 | 1997-04-02 | Innes John Centre Innov Ltd | Methods and means for gene silencing in transgenic plants |
| EP0979311A1 (en) | 1997-04-30 | 2000-02-16 | Of The University Of Minnesota Regents | $i(IN VIVO) USE OF RECOMBINAGENIC OLIGONUCLEOBASES TO CORRECT GENETIC LESIONS IN HEPATOCYTES |
| GB9710475D0 (en) | 1997-05-21 | 1997-07-16 | Zeneca Ltd | Gene silencing |
| EP1007712A4 (en) | 1997-08-05 | 2004-06-30 | Kimeragen Inc | USE OF DUPLEX OLIGONUCLEOTIDES TO AFFECT LOCAL GENETIC CHANGES IN PLANTS |
| ATE401410T1 (de) | 1997-11-18 | 2008-08-15 | Pioneer Hi Bred Int | Zusammensetzungen und verfahren für die genetische modifizierung von pflanzen |
| FR2772047B1 (fr) | 1997-12-05 | 2004-04-09 | Ct Nat D Etudes Veterinaires E | Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection |
| ES2374290T3 (es) | 1998-03-20 | 2012-02-15 | Commonwealth Scientific And Industrial Research Organisation | Genes sintéticos y constructos genéticos que comprenden los mismos. |
| EP1068311B2 (en) | 1998-04-08 | 2020-12-09 | Commonwealth Scientific and Industrial Research Organisation | Methods and means for obtaining modified phenotypes |
| AU4095999A (en) | 1998-05-22 | 1999-12-13 | Arizona Board Of Regents On Behalf Of The University Of Arizona, The | Cell cycle genes, proteins and uses thereof |
| AR020078A1 (es) | 1998-05-26 | 2002-04-10 | Syngenta Participations Ag | Metodo para alterar la expresion de un gen objetivo en una celula de planta |
| US7307149B2 (en) | 1998-08-17 | 2007-12-11 | Pioneer Hi-Bred International, Inc. | Maize cellulose synthases and uses thereof |
| DE69937352T2 (de) | 1998-08-17 | 2008-07-17 | Pioneer Hi-Bred International, Inc. | Maiszellulosesynthasen und deren verwendungen |
| US6518487B1 (en) | 1998-09-23 | 2003-02-11 | Pioneer Hi-Bred International, Inc. | Cyclin D polynucleotides, polypeptides and uses thereof |
| US6504083B1 (en) | 1998-10-06 | 2003-01-07 | Pioneer Hi-Bred International, Inc. | Maize Gos-2 promoters |
| AU3369900A (en) | 1999-02-19 | 2000-09-04 | General Hospital Corporation, The | Gene silencing |
| US6992237B1 (en) | 1999-04-16 | 2006-01-31 | Pioneer Hi-Bred International Inc. | Regulated expression of genes in plant seeds |
| US7531723B2 (en) | 1999-04-16 | 2009-05-12 | Pioneer Hi-Bred International, Inc. | Modulation of cytokinin activity in plants |
| US6645747B1 (en) | 1999-09-21 | 2003-11-11 | E. I. Du Pont De Nemours And Company | Cis-prenyltransferases from plants |
| ATE287964T1 (de) | 1999-11-17 | 2005-02-15 | Pioneer Hi Bred Int | Modulation der antwort einer pflanze auf abscisin säure |
| CN1261577C (zh) | 2000-06-16 | 2006-06-28 | 托马斯·施穆林 | 改变植物形态学、生物化学和生理学的方法 |
| ATE507300T1 (de) | 2000-06-23 | 2011-05-15 | Du Pont | Rekombinante konstrukte und ihre verwendung bei der reduzierung der genexpression |
| US20020048814A1 (en) | 2000-08-15 | 2002-04-25 | Dna Plant Technology Corporation | Methods of gene silencing using poly-dT sequences |
| US7462481B2 (en) | 2000-10-30 | 2008-12-09 | Verdia, Inc. | Glyphosate N-acetyltransferase (GAT) genes |
| AU2002344893A1 (en) * | 2001-07-10 | 2003-01-29 | Universite De Montreal | Plant transcriptional repressor, proteic nuclear factors binding thereto, and uses thereof |
| JP2005519607A (ja) | 2002-03-14 | 2005-07-07 | コモンウェルス サイエンティフィック アンド インダストリアル リサーチ オーガニゼーション | 改変された遺伝子サイレンシングｒｎａおよびその使用 |
| US20040123347A1 (en) | 2002-12-20 | 2004-06-24 | Hinchey Brendan S. | Water-deficit-inducible plant promoters |
| US7279615B2 (en) | 2003-06-16 | 2007-10-09 | Pioneer Hi-Bred International, Inc. | Floral transition genes in maize and uses thereof |
| AU2004265250C1 (en) * | 2003-06-23 | 2008-05-01 | Pioneer Hi-Bred International, Inc. | Engineering single-gene-controlled staygreen potential into plants |
| US7411112B2 (en) | 2003-10-09 | 2008-08-12 | Pioneer Hi-Bred International, Inc. | Maize promoter named CRWAQ81 |
| US7405074B2 (en) | 2004-04-29 | 2008-07-29 | Pioneer Hi-Bred International, Inc. | Glyphosate-N-acetyltransferase (GAT) genes |
| US20060064786A1 (en) | 2004-09-17 | 2006-03-23 | Pioneer Hi-Bred International, Inc. | Isopentenyl transferase sequences and methods of use |
| AU2006283504B2 (en) | 2005-08-24 | 2011-08-25 | E. I. Du Pont De Nemours And Company | Compositions providing tolerance to multiple herbicides and methods of use thereof |
| US20090229014A1 (en) | 2006-04-07 | 2009-09-10 | Xing Wang Deng | Drought and High Salinity Stress Induced Rice Promoters |
| WO2007131109A2 (en) | 2006-05-03 | 2007-11-15 | Plexus Biomedical, Inc. | Apparatus and method of inhibiting perianal tissue damage |
| US7902428B2 (en) | 2007-08-07 | 2011-03-08 | Pioneer Hi-Bred International, Inc. | Plant regulatory region that directs transgene expression in the maternal and supporting tissue of maize ovules and pollinated kernels |
| CA2727598A1 (en) | 2008-06-10 | 2009-12-17 | Pioneer Hi-Bred International, Inc. | Compositions and methods of use of mitogen-activated protein kinase kinase kinase |
| UA108071C2 (uk) * | 2009-04-14 | 2015-03-25 | Піонер Хай-Бред Інтернешнл, Інк. | Спосіб поліпшення витривалості до нестачі азоту у рослини |
-
2010
- 2010-10-04 WO PCT/US2010/051358 patent/WO2011041796A1/en not_active Ceased
- 2010-10-04 CN CN2010800434674A patent/CN102597229A/zh active Pending
- 2010-10-04 EP EP10787210A patent/EP2483404A1/en not_active Withdrawn
- 2010-10-04 MX MX2012003972A patent/MX2012003972A/es not_active Application Discontinuation
- 2010-10-04 IN IN2408DEN2012 patent/IN2012DN02408A/en unknown
- 2010-10-04 CA CA2775093A patent/CA2775093A1/en not_active Abandoned
- 2010-10-04 US US12/897,489 patent/US20110252505A1/en not_active Abandoned
- 2010-10-04 AR ARP100103605A patent/AR078502A1/es unknown
- 2010-10-04 BR BR112012007516A patent/BR112012007516A2/pt not_active IP Right Cessation
-
2013
- 2013-04-22 US US13/867,594 patent/US20130326730A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20110252505A1 (en) | 2011-10-13 |
| AR078502A1 (es) | 2011-11-09 |
| EP2483404A1 (en) | 2012-08-08 |
| IN2012DN02408A (enExample) | 2015-08-21 |
| CN102597229A (zh) | 2012-07-18 |
| WO2011041796A1 (en) | 2011-04-07 |
| BR112012007516A2 (pt) | 2015-09-15 |
| MX2012003972A (es) | 2012-05-08 |
| US20130326730A1 (en) | 2013-12-05 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9677084B2 (en) | Down-regulation of a homeodomain-leucine zipper I-class homeobox gene for improved plant performance | |
| US8987553B2 (en) | Modulation of ACC synthase improves plant yield under low nitrogen conditions | |
| US20100115662A1 (en) | Manipulation of glutamine synthetases (gs) to improve nitrogen use efficiency and grain yield in higher plants | |
| CN101421295A (zh) | 用于提高作物植物中的氮利用效率的基因 | |
| US20080222753A1 (en) | Manipulation of Ammonium Transporters (AMTS) to Improve Nitrogen Use Efficiency in Higher Plants | |
| US20160010101A1 (en) | Enhanced nitrate uptake and nitrate translocation by over- expressing maize functional low-affinity nitrate transporters in transgenic maize | |
| US20130326730A1 (en) | Down-regulation of acc synthase for improved plant performance | |
| US8269066B2 (en) | Nitrate reductases from red algae, compositions and methods of use thereof | |
| WO2014031675A2 (en) | Down-regulation of bzip transcription factor genes for improved plant performance | |
| US9701975B2 (en) | Down-regulation of auxin responsive genes for improved plant performance | |
| US20090158459A1 (en) | Novel transcription factor for increasing kernel mass and yield in plants (1403) | |
| US20160017360A1 (en) | Functional expression of bacterial major facilitator superfamily mfs gene in maize to improve agronomic traits and grain yield |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20161005 |