CA2766728A1 - Method for replicating, amplifying and sequencing a template dna - Google Patents
Method for replicating, amplifying and sequencing a template dna Download PDFInfo
- Publication number
- CA2766728A1 CA2766728A1 CA2766728A CA2766728A CA2766728A1 CA 2766728 A1 CA2766728 A1 CA 2766728A1 CA 2766728 A CA2766728 A CA 2766728A CA 2766728 A CA2766728 A CA 2766728A CA 2766728 A1 CA2766728 A1 CA 2766728A1
- Authority
- CA
- Canada
- Prior art keywords
- dna
- dna polymerase
- concentration
- ammonium
- phi
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES200930412A ES2351294B8 (es) | 2009-07-02 | 2009-07-02 | Metodo para la replicación, amplificación, o secuenciación de un adn molde. |
| ESP200930412 | 2009-07-02 | ||
| PCT/ES2010/070456 WO2011000998A2 (es) | 2009-07-02 | 2010-07-02 | Método para la replicación, amplificación o secuenciación de un adn molde |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2766728A1 true CA2766728A1 (en) | 2011-01-06 |
Family
ID=43411512
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2766728A Abandoned CA2766728A1 (en) | 2009-07-02 | 2010-07-02 | Method for replicating, amplifying and sequencing a template dna |
Country Status (17)
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014520544A (ja) * | 2011-07-14 | 2014-08-25 | ディーエヌエー エレクトロニクス エルティーディー | 核酸増幅 |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105483115A (zh) * | 2014-09-19 | 2016-04-13 | 深圳华大基因科技有限公司 | 一种用于单细胞全基因组扩增的试剂盒及其应用 |
| CN104328111A (zh) * | 2014-10-29 | 2015-02-04 | 北京大学 | 一种用于测序的高通量扩增质粒的方法 |
| CN105695571A (zh) * | 2016-01-29 | 2016-06-22 | 苏州金唯智生物科技有限公司 | 一种基于滚环扩增的dna定量方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5001050A (en) | 1989-03-24 | 1991-03-19 | Consejo Superior Investigaciones Cientificas | PHφ29 DNA polymerase |
| US5198543A (en) | 1989-03-24 | 1993-03-30 | Consejo Superior Investigaciones Cientificas | PHI29 DNA polymerase |
| US20030049614A1 (en) * | 1999-10-06 | 2003-03-13 | Holly Hurlbut Hogrefe | Compositions for dna amplification, synthesis, and mutagenesis |
| US6323009B1 (en) | 2000-06-28 | 2001-11-27 | Molecular Staging, Inc. | Multiply-primed amplification of nucleic acid sequences |
| EP1590479B1 (en) * | 2003-02-05 | 2010-11-24 | GE Healthcare Bio-Sciences Corp. | Nucleic acid amplification |
| JP4918409B2 (ja) * | 2006-07-26 | 2012-04-18 | 西川ゴム工業株式会社 | 核酸配列の増幅方法 |
| EP2108048A1 (en) * | 2007-01-17 | 2009-10-14 | Meridian Bioscience, Inc. | Stable reagents and kits useful in loop-mediated isothermal amplification (lamp) |
| GB0901593D0 (en) * | 2009-01-30 | 2009-03-11 | Touchlight Genetics Ltd | Production of closed linear DNA |
-
2009
- 2009-07-02 ES ES200930412A patent/ES2351294B8/es not_active Expired - Fee Related
-
2010
- 2010-07-02 MX MX2012000152A patent/MX2012000152A/es not_active Application Discontinuation
- 2010-07-02 DK DK10793650.2T patent/DK2450453T3/en active
- 2010-07-02 EP EP10793650.2A patent/EP2450453B1/en active Active
- 2010-07-02 US US13/381,845 patent/US20120178092A1/en not_active Abandoned
- 2010-07-02 AU AU2010267955A patent/AU2010267955A1/en not_active Abandoned
- 2010-07-02 CN CN2010800397389A patent/CN102648289A/zh active Pending
- 2010-07-02 JP JP2012519026A patent/JP2012531221A/ja active Pending
- 2010-07-02 KR KR1020127002885A patent/KR20120059492A/ko not_active Withdrawn
- 2010-07-02 BR BRPI1014001-8A patent/BRPI1014001A2/pt not_active IP Right Cessation
- 2010-07-02 WO PCT/ES2010/070456 patent/WO2011000998A2/es not_active Ceased
- 2010-07-02 SG SG2011097078A patent/SG177411A1/en unknown
- 2010-07-02 CA CA2766728A patent/CA2766728A1/en not_active Abandoned
- 2010-07-02 IN IN172DEN2012 patent/IN2012DN00172A/en unknown
-
2011
- 2011-12-26 CL CL2011003313A patent/CL2011003313A1/es unknown
- 2011-12-29 CO CO11180680A patent/CO6480969A2/es not_active Application Discontinuation
-
2012
- 2012-01-02 IL IL217325A patent/IL217325A0/en unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2014520544A (ja) * | 2011-07-14 | 2014-08-25 | ディーエヌエー エレクトロニクス エルティーディー | 核酸増幅 |
Also Published As
| Publication number | Publication date |
|---|---|
| US20120178092A1 (en) | 2012-07-12 |
| CL2011003313A1 (es) | 2012-06-08 |
| EP2450453A4 (en) | 2012-12-26 |
| CN102648289A (zh) | 2012-08-22 |
| ES2351294A1 (es) | 2011-02-02 |
| SG177411A1 (en) | 2012-02-28 |
| KR20120059492A (ko) | 2012-06-08 |
| MX2012000152A (es) | 2012-06-08 |
| EP2450453A2 (en) | 2012-05-09 |
| ES2351294B8 (es) | 2012-06-06 |
| IL217325A0 (en) | 2012-02-29 |
| DK2450453T3 (en) | 2017-08-21 |
| EP2450453B1 (en) | 2017-05-31 |
| CO6480969A2 (es) | 2012-07-16 |
| IN2012DN00172A (cg-RX-API-DMAC10.html) | 2015-04-17 |
| AU2010267955A1 (en) | 2012-02-16 |
| BRPI1014001A2 (pt) | 2018-01-30 |
| ES2351294B1 (es) | 2011-11-24 |
| WO2011000998A2 (es) | 2011-01-06 |
| WO2011000998A3 (es) | 2011-07-21 |
| JP2012531221A (ja) | 2012-12-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1147217B1 (en) | A method of dna sequencing | |
| EP3272879B1 (en) | Transposon end compositions and methods for modifying nucleic acids | |
| AU2001268725B2 (en) | Multiply-primed amplification of nucleic acid sequences | |
| JP6382189B2 (ja) | Ｒｎａテンプレートから開始する等温ｄｎａ増幅用のキット | |
| HK1249765A1 (en) | Transposon end compositions and methods for modifying nucleic acids | |
| CN106414737A (zh) | 维持拷贝数的rna分析方法 | |
| EP2450453B1 (en) | Method for the replication, amplification or sequencing of a dna template | |
| HK1173471A (en) | Method for the replication, amplification or sequencing of a dna template | |
| HK1219806B (en) | Transposon end compositions and methods for modifying nucleic acids | |
| HK1169659B (en) | Transposon end compositions and methods for modifying nucleic acids | |
| HK1163107B (en) | Transposon end compositions and methods for modifying nucleic acids | |
| HK1197498B (en) | Transposon end compositions and methods for modifying nucleic acids | |
| WO2002101041A1 (en) | Method of amplifying nucleic acid and method of detecting nucleotide polymorphism with the use of nucleotide analog |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |
Effective date: 20140702 |