CA2753922C - Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses - Google Patents

Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses Download PDF

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Publication number
CA2753922C
CA2753922C CA2753922A CA2753922A CA2753922C CA 2753922 C CA2753922 C CA 2753922C CA 2753922 A CA2753922 A CA 2753922A CA 2753922 A CA2753922 A CA 2753922A CA 2753922 C CA2753922 C CA 2753922C
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CA
Canada
Prior art keywords
nucleic acid
target
group
labeled
present
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2753922A
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English (en)
French (fr)
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CA2753922A1 (en
Inventor
Jannis G. Stavrianopoulos
Elazar Rabbani
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Enzo Life Sciences Inc
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Enzo Life Sciences Inc
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Filing date
Publication date
Application filed by Enzo Life Sciences Inc filed Critical Enzo Life Sciences Inc
Publication of CA2753922A1 publication Critical patent/CA2753922A1/en
Application granted granted Critical
Publication of CA2753922C publication Critical patent/CA2753922C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B23/00—Methine or polymethine dyes, e.g. cyanine dyes
    • C09B23/02—Methine or polymethine dyes, e.g. cyanine dyes the polymethine chain containing an odd number of >CH- or >C[alkyl]- groups
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B47/00—Porphines; Azaporphines
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B57/00—Other synthetic dyes of known constitution
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
CA2753922A 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses Expired - Lifetime CA2753922C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/096,075 2002-03-12
US10/096,075 US7166478B2 (en) 2002-03-12 2002-03-12 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
CA2421552A CA2421552C (en) 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2421552A Division CA2421552C (en) 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses

Publications (2)

Publication Number Publication Date
CA2753922A1 CA2753922A1 (en) 2003-09-12
CA2753922C true CA2753922C (en) 2016-06-28

Family

ID=27804274

Family Applications (7)

Application Number Title Priority Date Filing Date
CA2754185A Expired - Lifetime CA2754185C (en) 2002-03-12 2003-03-11 Chemiluminescent dioxetanes
CA2754186A Expired - Lifetime CA2754186C (en) 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
CA2753922A Expired - Lifetime CA2753922C (en) 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
CA2421552A Expired - Lifetime CA2421552C (en) 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
CA2754415A Expired - Lifetime CA2754415C (en) 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
CA2754041A Expired - Lifetime CA2754041C (en) 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
CA2753910A Expired - Lifetime CA2753910C (en) 2002-03-12 2003-03-11 Fluorescent dye composition and target labeled therewith

Family Applications Before (2)

Application Number Title Priority Date Filing Date
CA2754185A Expired - Lifetime CA2754185C (en) 2002-03-12 2003-03-11 Chemiluminescent dioxetanes
CA2754186A Expired - Lifetime CA2754186C (en) 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses

Family Applications After (4)

Application Number Title Priority Date Filing Date
CA2421552A Expired - Lifetime CA2421552C (en) 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
CA2754415A Expired - Lifetime CA2754415C (en) 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
CA2754041A Expired - Lifetime CA2754041C (en) 2002-03-12 2003-03-11 Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
CA2753910A Expired - Lifetime CA2753910C (en) 2002-03-12 2003-03-11 Fluorescent dye composition and target labeled therewith

Country Status (5)

Country Link
US (11) US7166478B2 (US07553959-20090630-C00018.png)
EP (1) EP1348713B1 (US07553959-20090630-C00018.png)
JP (3) JP4551063B2 (US07553959-20090630-C00018.png)
CA (7) CA2754185C (US07553959-20090630-C00018.png)
IL (13) IL195437A (US07553959-20090630-C00018.png)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9261460B2 (en) * 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US20060073149A1 (en) * 2001-08-17 2006-04-06 Bales Kelly R Rapid improvement of cognition in condition related to abeta
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses
US9353405B2 (en) 2002-03-12 2016-05-31 Enzo Life Sciences, Inc. Optimized real time nucleic acid detection processes
DE60325717D1 (de) * 2002-04-25 2009-02-26 Lilly Co Eli Verfahren zur behandlung von angststörungen bei älteren personen
US7575747B2 (en) * 2003-02-10 2009-08-18 Applied Molecular Evolution Aβ binding molecules
US8399196B2 (en) * 2003-02-21 2013-03-19 Geneform Technologies Limited Nucleic acid sequencing methods, kits and reagents
US7514551B2 (en) * 2003-04-03 2009-04-07 Enzo Life Sciences, Inc. Multisignal labeling reagents, and processes and uses therefor
US9156986B2 (en) 2003-04-03 2015-10-13 Enzo Life Sciences, Inc. Multisignal labeling reagents and processes and uses therefor
US9696298B2 (en) 2003-04-03 2017-07-04 Enzo Life Sciences, Inc. Multisignal reagents for labeling analytes
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
ITPZ20030002A1 (it) * 2003-08-12 2005-02-13 Giuseppe Caputo Composti del tipo cianina aventi un braccio di legame alchinilico
GB0324456D0 (en) * 2003-10-20 2003-11-19 Isis Innovation Parallel DNA sequencing methods
US8124334B2 (en) * 2004-07-06 2012-02-28 Enzo Biochem, Inc. Selective detection of oncogenic HPV
US7601498B2 (en) * 2005-03-17 2009-10-13 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection and associated technology
US7776567B2 (en) * 2005-03-17 2010-08-17 Biotium, Inc. Dimeric and trimeric nucleic acid dyes, and associated systems and methods
US7569695B2 (en) * 2005-05-24 2009-08-04 Enzo Life Sciences, Inc. Dyes for the detection or quantification of desirable target molecules
US8357801B2 (en) * 2005-05-24 2013-01-22 Enzo Life Sciences, Inc. Labeling of target molecules, identification of organelles and other applications, novel compositions, methods and kits
US7737281B2 (en) * 2005-05-24 2010-06-15 Enzo Life Sciences, Inc. C/O Enzo Biochem, Inc. Purine based fluorescent dyes
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
US20070141597A1 (en) * 2005-10-25 2007-06-21 Harmon H J Biomimetic Biodetector of Toxins, Viruses, Bacteria, and Biological Factors
US7781187B2 (en) * 2005-12-30 2010-08-24 Corning Incorporated Fluorescent dyes
JP2007215496A (ja) * 2006-02-17 2007-08-30 Filgen Inc 核酸解析法におけるプローブ核酸の蛍光標識方法
US20080091005A1 (en) * 2006-07-20 2008-04-17 Visigen Biotechnologies, Inc. Modified nucleotides, methods for making and using same
US7629125B2 (en) * 2006-11-16 2009-12-08 General Electric Company Sequential analysis of biological samples
EP2121754B1 (en) * 2007-01-18 2015-02-11 Eli Lilly and Company Pegylated amyloid beta fab
US7485737B2 (en) * 2007-02-06 2009-02-03 Xerox Corporation Colorant compounds
US7910754B2 (en) 2007-02-06 2011-03-22 Xerox Corporation Colorant compounds
US20090162845A1 (en) * 2007-12-20 2009-06-25 Elazar Rabbani Affinity tag nucleic acid and protein compositions, and processes for using same
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
KR102508594B1 (ko) * 2008-12-10 2023-03-09 위스타 레보레이토리스 리미티드 의약으로서 3,6-이중치환된 크산틸륨 염
US9133343B2 (en) 2009-11-30 2015-09-15 Enzo Biochem, Inc. Dyes and compositions, and processes for using same in analysis of protein aggregation and other applications
US8877437B1 (en) 2009-12-23 2014-11-04 Biotium, Inc. Methods of using dyes in association with nucleic acid staining or detection
US9493824B2 (en) 2010-12-16 2016-11-15 Bio-Rad Laboratories, Inc. Universal reference dye for quantitative amplification
CN103597039B (zh) 2011-03-15 2017-05-03 雷蒙特亚特特拉维夫大学有限公司 可活化的发荧光的化合物及其作为近红外探针的用途
US20120316319A1 (en) * 2011-06-10 2012-12-13 Alexey Ustinov Arylethynylxanthene dyes
AU2012309824A1 (en) 2011-09-12 2013-04-18 Abbvie Biotherapeutics Inc. Artificial NK cells and uses thereof
US9416153B2 (en) 2011-10-11 2016-08-16 Enzo Life Sciences, Inc. Fluorescent dyes
US10060910B2 (en) 2012-07-24 2018-08-28 Enzo Life Sciences, Inc. Multisignal reagents for labeling analytes
WO2014027652A1 (ja) 2012-08-17 2014-02-20 独立行政法人科学技術振興機構 ラマン分光法を用いた生体分子の解析方法及び装置
EP2976622B1 (en) * 2013-03-14 2019-11-27 Bio-Rad Laboratories, Inc. Protein standard
US9957393B2 (en) 2015-03-30 2018-05-01 Enzo Biochem, Inc. Monoazo dyes with cyclic amine as fluorescence quenchers
US9969887B2 (en) 2016-04-20 2018-05-15 Sfc Co., Ltd. Merocyanine-based compounds, and dyes, kits and contrast medium compositions for labelling biomolecules comprising the same
US11377563B2 (en) 2016-06-06 2022-07-05 Sony Corporation Ionic polymers comprising fluorescent or colored reporter groups
CN110426442B (zh) * 2016-06-13 2022-02-11 苏州优逸兰迪生物科技有限公司 一种核酸凝胶电泳测试的方法
CN108047135B (zh) * 2017-12-29 2019-12-17 苏州宇恒生物科技有限公司 一种荧光染料及其制备方法与在细菌检测中的应用
US11384376B2 (en) 2018-05-31 2022-07-12 Roche Molecular Systems, Inc. Reagents and methods for post-synthetic modification of nucleic acids
WO2021123074A1 (en) 2019-12-18 2021-06-24 F. Hoffmann-La Roche Ag Methods of sequencing by synthesis using a consecutive labeling scheme
CN116926178A (zh) * 2022-04-02 2023-10-24 深圳市真迈生物科技有限公司 荧光检测试剂及其制备方法及应用

Family Cites Families (93)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4375972A (en) * 1979-10-17 1983-03-08 Allied Corporation Heterogeneous chemiluminescent immunoassays utilizing metallo porphyrin tag
US4711955A (en) * 1981-04-17 1987-12-08 Yale University Modified nucleotides and methods of preparing and using same
CA1190838A (en) 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
CA1186621A (en) * 1981-08-10 1985-05-07 John L. Hendrix Fluoro immuno assay system
US4707454A (en) * 1981-08-10 1987-11-17 Bio-Diagnostics, Inc. Fluorescent chlorophyll labeled assay reagents
US5241060A (en) * 1982-06-23 1993-08-31 Enzo Diagnostics, Inc. Base moiety-labeled detectable nucleatide
US4994373A (en) * 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
IL70765A (en) * 1983-01-27 1988-07-31 Enzo Biochem Inc Substrates containing non-radioactive chemically-labeled polynucleotides and methods using them
US4952685A (en) 1984-01-30 1990-08-28 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US5013831A (en) * 1984-01-30 1991-05-07 Enzo Biochem, Inc. Detectable molecules, method of preparation and use
US6221581B1 (en) * 1984-04-27 2001-04-24 Enzo Diagnostics, Inc. Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions
US5288609A (en) * 1984-04-27 1994-02-22 Enzo Diagnostics, Inc. Capture sandwich hybridization method and composition
CA1260372A (en) * 1984-04-27 1989-09-26 Elazar Rabbani Hybridization method for the detection of genetic materials
EP0187332B1 (en) * 1985-01-10 1989-02-01 Molecular Diagnostics, Inc. Photochemical method of labelling nucleic acids for detection in hybridization assays
US5104637A (en) * 1985-02-06 1992-04-14 University Of Cincinnati Radio labeled dihematophorphyrin ether and its use in detecting and treating neoplastic tissue
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4824659A (en) * 1985-06-07 1989-04-25 Immunomedics, Inc. Antibody conjugates
US4868103A (en) * 1986-02-19 1989-09-19 Enzo Biochem, Inc. Analyte detection by means of energy transfer
US5627027A (en) * 1986-04-18 1997-05-06 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
US5268486A (en) * 1986-04-18 1993-12-07 Carnegie-Mellon Unversity Method for labeling and detecting materials employing arylsulfonate cyanine dyes
US6225050B1 (en) * 1986-04-18 2001-05-01 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
US5047519A (en) * 1986-07-02 1991-09-10 E. I. Du Pont De Nemours And Company Alkynylamino-nucleotides
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
US5707559A (en) * 1986-07-17 1998-01-13 Tropix, Inc. Chemiluminescent 1,2-dioxetane compounds
US4978614A (en) * 1988-10-26 1990-12-18 Tropix, Inc. Method of detecting a substance using enzymatically-induced decomposition of dioxetanes
US4952686A (en) * 1987-12-01 1990-08-28 Fmc Corporation Soluble dried cassia alloy gum composition and process for making same
US5130238A (en) * 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
JPH0257978A (ja) * 1988-08-24 1990-02-27 Takara Shuzo Co Ltd Ｄｎａ塩基配列決定方法
US5118801A (en) * 1988-09-30 1992-06-02 The Public Health Research Institute Nucleic acid process containing improved molecular switch
US5132204A (en) * 1989-05-31 1992-07-21 Chiron Corporation Chemiluminescent double-triggered 1, 2-dioxetanes
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
FR2656866B1 (fr) * 1990-01-10 1992-05-15 Cis Bio Int Derives de porphyrine et metalloporphyrines eventuellement couples a une molecule biologiquement active, et composition pharmaceutique les contenant.
US5401847A (en) * 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
US5494810A (en) * 1990-05-03 1996-02-27 Cornell Research Foundation, Inc. Thermostable ligase-mediated DNA amplifications system for the detection of genetic disease
US5455175A (en) * 1990-06-04 1995-10-03 University Of Utah Research Foundation Rapid thermal cycling device
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5994056A (en) * 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5213831A (en) * 1991-05-06 1993-05-25 Brown's Best Food Processing system and method for quick-cooking legumes
US5248618A (en) * 1991-06-05 1993-09-28 Life Technologies, Inc. Methods for generating light with chemiluminescent dioxetanes activated by anchimeric assisted cleavage
IL102523A0 (en) * 1991-07-31 1993-01-14 Du Pont Enhancing the chemiluminescence of enzyme-triggered 1,2-dioxetanes
JP2914405B2 (ja) * 1991-08-07 1999-06-28 日石三菱株式会社 標識されたポルフィリン化合物の製造方法
ATE189831T1 (de) * 1991-11-14 2000-03-15 Dgi Inc Nicht-radioaktives hybridisations testverfahren und satz
US5270184A (en) * 1991-11-19 1993-12-14 Becton, Dickinson And Company Nucleic acid target generation
US5565552A (en) * 1992-01-21 1996-10-15 Pharmacyclics, Inc. Method of expanded porphyrin-oligonucleotide conjugate synthesis
US5393469A (en) * 1992-03-20 1995-02-28 Lumigen, Inc. Polymeric phosphonium salts providing enhanced chemiluminescence from 1,2-dioxetanes
CA2135073C (en) * 1992-05-06 2002-11-19 Daniel L. Kacian Nucleic acid sequence amplification method, composition and kit
US5340714A (en) * 1992-05-08 1994-08-23 Monitor Diagnostics, Inc. Use of nonmetallic tetrapyrrole molecules and novel signal solutions in chemiluminescent reactions and assays
US5462854A (en) * 1993-04-19 1995-10-31 Beckman Instruments, Inc. Inverse linkage oligonucleotides for chemical and enzymatic processes
FR2706618B1 (fr) * 1993-06-11 1995-09-01 Bio Merieux Dispositif pour le dosage d'haptènes et son utilisation.
US5428149A (en) * 1993-06-14 1995-06-27 Washington State University Research Foundation Method for palladium catalyzed carbon-carbon coulping and products
ATE264307T1 (de) * 1993-06-30 2004-04-15 Abbott Lab Interkalatoren mit affinität für dna und ihre anwendung
US5808077A (en) * 1993-06-30 1998-09-15 Abbott Laboratories Intercalators having affinity for DNA and methods of use
JPH0794807A (ja) * 1993-07-27 1995-04-07 Toshiba Corp アモルファス有機薄膜素子、アモルファス有機ポリマー組成物及びアモルファス無機組成物
US5464741A (en) * 1993-10-08 1995-11-07 Henwell, Inc. Palladium (II) octaethylporphine alpha-isothiocyanate as a phosphorescent label for immunoassays
CA2140081C (en) 1994-01-13 2008-04-01 Dean L. Engelhardt Process, construct and conjugate for producing multiple nucleic acid copies
US5578832A (en) * 1994-09-02 1996-11-26 Affymetrix, Inc. Method and apparatus for imaging a sample on a device
DE4407423A1 (de) * 1994-03-05 1995-09-07 Boehringer Mannheim Gmbh Entstörmittel zum Einsatz bei Immunoassays
US6589736B1 (en) * 1994-11-22 2003-07-08 The Trustees Of Boston University Photocleavable agents and conjugates for the detection and isolation of biomolecules
US6008373A (en) * 1995-06-07 1999-12-28 Carnegie Mellon University Fluorescent labeling complexes with large stokes shift formed by coupling together cyanine and other fluorochromes capable of resonance energy transfer
US5827073A (en) * 1995-07-05 1998-10-27 Ludwig Institute For Cancer Research Photoreactive peptide derivatives
US5929227A (en) * 1995-07-12 1999-07-27 The Regents Of The University Of California Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
US5631167A (en) * 1995-07-31 1997-05-20 Bayer Corporation Capsule chemistry analytical methods employing dioxetane chemiluminescence
US5945283A (en) * 1995-12-18 1999-08-31 Washington University Methods and kits for nucleic acid analysis using fluorescence resonance energy transfer
GB9605690D0 (en) * 1996-03-19 1996-05-22 Oxford Biosciences Ltd Particle delivery
US5945526A (en) * 1996-05-03 1999-08-31 Perkin-Elmer Corporation Energy transfer dyes with enhanced fluorescence
US5800996A (en) * 1996-05-03 1998-09-01 The Perkin Elmer Corporation Energy transfer dyes with enchanced fluorescence
ATE318327T1 (de) * 1996-06-04 2006-03-15 Univ Utah Res Found Fluoreszenz-donor-akzeptor paar
US6001573A (en) * 1996-06-14 1999-12-14 Packard Bioscience B.V. Use of porphyrins as a universal label
US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
US5730849A (en) * 1996-09-30 1998-03-24 Bio-Rad Laboratories, Inc. High sensitivity dyes as stains for RNA bands in denaturing gels
USRE40557E1 (en) * 1996-10-08 2008-10-28 Kreatech Biotechnology B.V. Methods for labeling nucleotides, labeled nucleotides and useful intermediates
US5800999A (en) * 1996-12-16 1998-09-01 Tropix, Inc. Dioxetane-precursor-labeled probes and detection assays employing the same
US6506895B2 (en) * 1997-08-15 2003-01-14 Surmodics, Inc. Photoactivatable nucleic acids
US6008379A (en) * 1997-10-01 1999-12-28 The Perkin-Elmer Corporation Aromatic-substituted xanthene dyes
JP3595137B2 (ja) 1997-11-14 2004-12-02 シャープ株式会社 液晶タブレット装置
GB9725197D0 (en) 1997-11-29 1998-01-28 Secr Defence Detection system
US6080868A (en) * 1998-01-23 2000-06-27 The Perkin-Elmer Corporation Nitro-substituted non-fluorescent asymmetric cyanine dye compounds
EP1170381A3 (en) * 1998-02-27 2004-01-28 PamGene B.V. Method for the non-specific amplification of nucleic acid
US6068979A (en) * 1998-04-22 2000-05-30 Lumigen, Inc. Simplified sequential chemiluminescent detection
US5948648A (en) * 1998-05-29 1999-09-07 Khan; Shaheer H. Nucleotide compounds including a rigid linker
US6110630A (en) * 1998-06-18 2000-08-29 Beckman Coulter, Inc. Efficient activated cyanine dyes
WO2000006164A1 (en) * 1998-07-28 2000-02-10 Tropix, Inc. Benzothiazole dioxetanes
US6288221B1 (en) * 1998-08-20 2001-09-11 Duke University Methods of synthesis of halogen base-modified oligonucleotides and subsequent labeling with a metal-catalyzed reaction
US6114350A (en) * 1999-04-19 2000-09-05 Nen Life Science Products, Inc. Cyanine dyes and synthesis methods thereof
US6403807B1 (en) * 1999-07-06 2002-06-11 Surromed, Inc. Bridged fluorescent dyes, their preparation and their use in assays
US6716994B1 (en) * 2000-01-04 2004-04-06 Applera Corporation Mobility-Modifying Cyanine Dyes
US6323337B1 (en) * 2000-05-12 2001-11-27 Molecular Probes, Inc. Quenching oligonucleotides
EP1790736A3 (en) * 2000-10-06 2007-08-15 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding DNA and RNA
US6887690B2 (en) * 2001-06-22 2005-05-03 Pe Corporation Dye-labeled ribonucleotide triphosphates
US20040161741A1 (en) 2001-06-30 2004-08-19 Elazar Rabani Novel compositions and processes for analyte detection, quantification and amplification
US9261460B2 (en) 2002-03-12 2016-02-16 Enzo Life Sciences, Inc. Real-time nucleic acid detection processes and compositions
US7166478B2 (en) * 2002-03-12 2007-01-23 Enzo Life Sciences, Inc., C/O Enzo Biochem, Inc. Labeling reagents and labeled targets, target labeling processes and other processes for using same in nucleic acid determinations and analyses

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