CA2726260C - Procede de synthese d'arn par voie chimique - Google Patents

Procede de synthese d'arn par voie chimique Download PDF

Info

Publication number
CA2726260C
CA2726260C CA2726260A CA2726260A CA2726260C CA 2726260 C CA2726260 C CA 2726260C CA 2726260 A CA2726260 A CA 2726260A CA 2726260 A CA2726260 A CA 2726260A CA 2726260 C CA2726260 C CA 2726260C
Authority
CA
Canada
Prior art keywords
group
formula
natural
modified
protected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2726260A
Other languages
English (en)
French (fr)
Other versions
CA2726260A1 (fr
Inventor
Francoise Debart
Jean-Jacques Vasseur
Thomas Lavergne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite de Montpellier
Original Assignee
Centre National de la Recherche Scientifique CNRS
Universite de Montpellier
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centre National de la Recherche Scientifique CNRS, Universite de Montpellier filed Critical Centre National de la Recherche Scientifique CNRS
Publication of CA2726260A1 publication Critical patent/CA2726260A1/fr
Application granted granted Critical
Publication of CA2726260C publication Critical patent/CA2726260C/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06—Pyrimidine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16—Purine radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CA2726260A 2008-05-29 2009-05-28 Procede de synthese d'arn par voie chimique Active CA2726260C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0802928A FR2931824B1 (fr) 2008-05-29 2008-05-29 Procede de synthese d'arn par voie chimique.
FR0802928 2008-05-29
PCT/FR2009/000624 WO2009144418A1 (fr) 2008-05-29 2009-05-28 Procede de synthese d'arn par voie chimique

Publications (2)

Publication Number Publication Date
CA2726260A1 CA2726260A1 (fr) 2009-12-03
CA2726260C true CA2726260C (fr) 2017-04-04

Family

ID=39712442

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2726260A Active CA2726260C (fr) 2008-05-29 2009-05-28 Procede de synthese d'arn par voie chimique

Country Status (7)

Country Link
US (1) US8536318B2 (https=)
EP (1) EP2303905B1 (https=)
JP (1) JP5689054B2 (https=)
BR (1) BRPI0910689A2 (https=)
CA (1) CA2726260C (https=)
FR (1) FR2931824B1 (https=)
WO (1) WO2009144418A1 (https=)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011028218A1 (en) 2009-09-02 2011-03-10 Alnylam Pharmaceuticals, Inc. Process for triphosphate oligonucleotide synthesis
WO2012019168A2 (en) 2010-08-06 2012-02-09 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
RU2013120302A (ru) 2010-10-01 2014-11-20 Модерна Терапьютикс, Инк. Сконструированные нуклеиновые кислоты и способы их применения
EP2691101A2 (en) 2011-03-31 2014-02-05 Moderna Therapeutics, Inc. Delivery and formulation of engineered nucleic acids
US9464124B2 (en) 2011-09-12 2016-10-11 Moderna Therapeutics, Inc. Engineered nucleic acids and methods of use thereof
RU2648950C2 (ru) 2011-10-03 2018-04-02 Модерна Терапьютикс, Инк. Модифицированные нуклеозиды, нуклеотиды и нуклеиновые кислоты и их применение
CN104114572A (zh) 2011-12-16 2014-10-22 现代治疗公司 经修饰的核苷、核苷酸和核酸组合物
US9283287B2 (en) 2012-04-02 2016-03-15 Moderna Therapeutics, Inc. Modified polynucleotides for the production of nuclear proteins
US9572897B2 (en) 2012-04-02 2017-02-21 Modernatx, Inc. Modified polynucleotides for the production of cytoplasmic and cytoskeletal proteins
AU2013243948A1 (en) 2012-04-02 2014-10-30 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins associated with human disease
US9254311B2 (en) 2012-04-02 2016-02-09 Moderna Therapeutics, Inc. Modified polynucleotides for the production of proteins
US9303056B2 (en) 2012-07-25 2016-04-05 Kochi University Monomer for synthesis of RNA, method for producing same, and method for producing RNA
WO2014081507A1 (en) 2012-11-26 2014-05-30 Moderna Therapeutics, Inc. Terminally modified rna
US8980864B2 (en) 2013-03-15 2015-03-17 Moderna Therapeutics, Inc. Compositions and methods of altering cholesterol levels
PL221806B1 (pl) * 2013-03-21 2016-05-31 Inst Chemii Bioorg Polskiej Akademii Nauk Sposób wprowadzania acetalowych i acetaloestrowych grup ochronnych oraz związki do realizacji tego sposobu
US20160194625A1 (en) 2013-09-03 2016-07-07 Moderna Therapeutics, Inc. Chimeric polynucleotides
US10023626B2 (en) 2013-09-30 2018-07-17 Modernatx, Inc. Polynucleotides encoding immune modulating polypeptides
JP2016538829A (ja) 2013-10-03 2016-12-15 モデルナ セラピューティクス インコーポレイテッドＭｏｄｅｒｎａ Ｔｈｅｒａｐｅｕｔｉｃｓ，Ｉｎｃ． 低密度リポタンパク質受容体をコードするポリヌクレオチド
EP3062798B1 (en) 2013-11-01 2020-05-06 CureVac AG Modified rna with decreased immunostimulatory properties
ES2806575T3 (es) 2013-11-01 2021-02-18 Curevac Ag ARN modificado con propiedades inmunoestimuladoras disminuidas
EP3169693B1 (en) 2014-07-16 2022-03-09 ModernaTX, Inc. Chimeric polynucleotides
WO2023097308A1 (en) * 2021-11-29 2023-06-01 Hongene Biotech Corporation Synthesis of 2' acetyl-ester protected nucleosides
CN114685572B (zh) * 2022-06-02 2022-08-30 上海百力格生物技术有限公司 用于mgb核酸探针合成的氧化剂组合物及探针的合成方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
EP1386925A1 (en) * 2002-07-31 2004-02-04 Girindus AG Method for preparing oligonucleotides
CN100484949C (zh) * 2005-07-18 2009-05-06 张必良 用于rna寡核苷酸合成中的核苷亚磷酰胺及其合成方法

Also Published As

Publication number Publication date
JP2011521930A (ja) 2011-07-28
EP2303905A1 (fr) 2011-04-06
WO2009144418A1 (fr) 2009-12-03
JP5689054B2 (ja) 2015-03-25
BRPI0910689A2 (pt) 2016-11-01
FR2931824B1 (fr) 2014-11-28
US20110275793A1 (en) 2011-11-10
US8536318B2 (en) 2013-09-17
EP2303905B1 (fr) 2012-11-28
CA2726260A1 (fr) 2009-12-03
FR2931824A1 (fr) 2009-12-04

Similar Documents

Publication Publication Date Title
CA2726260C (fr) Procede de synthese d'arn par voie chimique
US5705629A (en) Methods for H-phosphonate synthesis of mono- and oligonucleotides
CA2561741C (en) Processes and reagents for oligonucleotide synthesis and purification
KR100782896B1 (ko) Ｌ-리보-ｌｎａ 유사체
CA2878945A1 (en) Chiral control
US8901289B2 (en) Preparation of nucleotide oligomer
Beaucage Solid-phase synthesis of siRNA oligonucleotides
WO1998008858A1 (en) Improved reagents and process for synthesis of oligonucleotides containing phosphorodithioate internucleoside linkages
NO319860B1 (no) Metylfosfonsyre-ester, fremgangsmate for fremstilling, legemiddel samt anvendelse
JPH11510790A (ja) オリゴヌクレオチド合成のための改良された方法
EP0290583B1 (fr) OLIGONUCLEOTIDES $g(a)
JPH0787982A (ja) 修飾オリゴデオキシリボヌクレオチド
NZ520778A (en) Process for the preparation of phosphorothioate triesters
US5807837A (en) Composition and method for the treatment or prophylaxis of viral infections using modified oligodeoxyribonucleotides
KR102097053B1 (ko) 동위체를 갖는 인산 화합물의 제조 방법
US6509459B1 (en) Base protecting groups and rapid process for oligonucleotide synthesis
US5639875A (en) Methods for H-phosphonate syntheis of oligonucleotides using triphosgene
Arnold Synthetic Nucleosides for the Elucidation of Biochemical Pathways from Alarmones to RNA Maintenance
JP4805143B2 (ja) ２‘水酸基を修飾された新規人工ｒｎａ
Thorpe Reviving H-phosphonate Chemistry: Novel Methods for Sustainable Oligonucleotide Synthesis
AU2011203074B2 (en) Processes and reagents for oligonucleotide synthesis and purification
Damha Developments in the chemical synthesis of nucleic acids and their analogues
WO1998033806A1 (en) Base protecting groups and process for oligonucleotide synthesis
YrxrN AND PHOSPHONATE DERIVATIVES
FR2612930A1 (fr) Sondes oligonucleotidiques a

Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20140408

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-4-4-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20251023

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 16TH ANNIV.) - STANDARD

Year of fee payment: 16

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20251122

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20251123

W00 Other event occurred

Free format text: ST27 STATUS EVENT CODE: A-4-4-W10-W00-W100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: LETTER SENT

Effective date: 20260224