CA2724076C - Expression heterologue de cellobiohydrolases fongiques dans la levure - Google Patents
Expression heterologue de cellobiohydrolases fongiques dans la levure Download PDFInfo
- Publication number
- CA2724076C CA2724076C CA2724076A CA2724076A CA2724076C CA 2724076 C CA2724076 C CA 2724076C CA 2724076 A CA2724076 A CA 2724076A CA 2724076 A CA2724076 A CA 2724076A CA 2724076 C CA2724076 C CA 2724076C
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- Prior art keywords
- codons
- nucleic acid
- codon
- acid sequence
- polynucleotide
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/06—Ethanol, i.e. non-beverage
- C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
- C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/02—Monosaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/52—Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
L'invention concerne l'expression hétérologue de polypeptides codés par des variants de type sauvage et optimisés par codon de cbhl et/ou cbh2 provenant d'organismes fongiques Talaromyces emersonii (T. emersonii), Humicola grisea (H. grisea), Thermoascus aurantiacus (T. aurantiacus), et Trichoderma reesei (T. reesei), dans des cellules hôtes telles que la levure Saccharomyces cerevisiae. L'expression des gènes correspondants, des variants et de leurs combinaisons, dans de telles cellules hôtes, se traduit par une activité spécifique améliorée des cellobiohydrolases exprimées. Ainsi, de tel gènes et systèmes d'expression sont utiles pour des systèmes de bio-traitement consolidés, efficaces et économiques.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CA3021166A CA3021166C (fr) | 2008-05-11 | 2009-05-11 | Expression heterologue de cellobiohydrolases fongiques dans la levure |
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US5221308P | 2008-05-11 | 2008-05-11 | |
US61/052,213 | 2008-05-11 | ||
PCT/IB2009/005881 WO2009138877A2 (fr) | 2008-05-11 | 2009-05-11 | Expression hétérologue de cellobiohydrolases fongiques dans la levure |
Related Child Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA3021166A Division CA3021166C (fr) | 2008-05-11 | 2009-05-11 | Expression heterologue de cellobiohydrolases fongiques dans la levure |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2724076A1 CA2724076A1 (fr) | 2009-11-19 |
CA2724076C true CA2724076C (fr) | 2018-11-27 |
Family
ID=41151793
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA3021166A Active CA3021166C (fr) | 2008-05-11 | 2009-05-11 | Expression heterologue de cellobiohydrolases fongiques dans la levure |
CA2724076A Active CA2724076C (fr) | 2008-05-11 | 2009-05-11 | Expression heterologue de cellobiohydrolases fongiques dans la levure |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA3021166A Active CA3021166C (fr) | 2008-05-11 | 2009-05-11 | Expression heterologue de cellobiohydrolases fongiques dans la levure |
Country Status (4)
Country | Link |
---|---|
US (2) | US9365842B2 (fr) |
BR (1) | BRPI0911966B1 (fr) |
CA (2) | CA3021166C (fr) |
WO (1) | WO2009138877A2 (fr) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11530428B2 (en) | 2008-05-11 | 2022-12-20 | Stellenbosch University | Nucleic acids encoding fungal cellobiohydrolases for expression in yeast |
Families Citing this family (24)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2009146464A2 (fr) * | 2008-05-30 | 2009-12-03 | Edenspace Systems Corporation | Systèmes de réduction de la récalcitrance de la biomasse |
CA3092340A1 (fr) | 2008-11-21 | 2010-05-27 | Universiteit Stellenbosch | Levure exprimant des cellulases pour saccharification et fermentation simultanees utilisant la cellulose |
BR112012011355A2 (pt) | 2009-10-26 | 2015-09-15 | Universiteit Stellenbosch Also Konown As Stellenbosch University | expressao heterologa de genes de celobiohidrolase 2 fungicos em levedura |
PL2357227T3 (pl) * | 2010-02-11 | 2015-12-31 | Sued Chemie Ip Gmbh & Co Kg | Zoptymalizowane enzymy celulazy |
AU2011214327B2 (en) * | 2010-02-11 | 2014-09-18 | Dsm Ip Assets B.V. | Polypeptide having cellobiohydrolase activity and uses thereof |
US9605269B2 (en) | 2010-05-05 | 2017-03-28 | Lallemand Hungary Liquidity Management Llc | Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate |
US8962295B2 (en) | 2010-06-01 | 2015-02-24 | California Institute Of Technology | Stable, functional chimeric cellobiohydrolase class I enzymes |
JP2014502144A (ja) * | 2010-10-06 | 2014-01-30 | ビーピー・コーポレーション・ノース・アメリカ・インコーポレーテッド | バリアントcbhiポリペプチド |
WO2012103350A1 (fr) * | 2011-01-26 | 2012-08-02 | Novozymes A/S | Polypeptides ayant une activité cellobiohydrolase et polynucléotides codant pour ceux-ci |
MX337919B (es) * | 2011-01-26 | 2016-03-28 | Novozymes As | Polipeptidos que tienen actividad de celobiohidrolasa y polinucleotidos que codifican los mismos. |
BR112013019039A2 (pt) | 2011-01-26 | 2020-07-21 | Novozymes A/S | Célula hospedeira microbiana transgênica, métodos para produzir um polipeptídeo, para produzir um mutante de uma célula originária, para produzir uma proteína, para degradar um material celulósico, para produzir um produto de fermentação, 5 e parafermentar um material celulósico, construção de ácido nucleico ou vetor de expressão, polipeptídeo isolado, e polinucleotídeo isolado |
CN103620028B (zh) | 2011-01-26 | 2017-05-03 | 诺维信公司 | 具有纤维二糖水解酶活性的多肽和编码该多肽的多核苷酸 |
WO2012138942A1 (fr) | 2011-04-05 | 2012-10-11 | Mascoma Corporation | Procédés pour l'amélioration du rendement et de la production de produit dans un microorganisme par l'addition d'accepteurs d'électrons alternatifs |
CA2878019A1 (fr) | 2011-12-20 | 2013-06-27 | Novozymes, Inc. | Variants de cellobiohydrolase et polynucleotides codant pour ceux-ci |
BR112014031526A2 (pt) * | 2012-06-19 | 2017-08-01 | Dsm Ip Assets Bv | promotores para expressar um gene em uma célula |
EP3290515A1 (fr) | 2012-12-12 | 2018-03-07 | Danisco US Inc. | Variants de cellobiohydrolases |
BR112015023451A2 (pt) | 2013-03-15 | 2017-12-05 | Lallemand Hungary Liquidity Man Llc | expressão de beta-glucosidases para hidrólise de lignocelulose e oligômeros associados |
US9850512B2 (en) | 2013-03-15 | 2017-12-26 | The Research Foundation For The State University Of New York | Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield |
WO2015017256A1 (fr) | 2013-07-29 | 2015-02-05 | Danisco Us Inc. | Enzymes variantes |
MX2016001881A (es) | 2013-08-15 | 2016-08-03 | Lallemand Hungary Liquidity Man Llc | Metodos para la mejora de rendimiento y produccion de producto en un microorganismo a traves del reciclaje de glicerol. |
US9951363B2 (en) | 2014-03-14 | 2018-04-24 | The Research Foundation for the State University of New York College of Environmental Science and Forestry | Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects |
BR122022015725B1 (pt) | 2017-05-23 | 2023-03-14 | Lallemand Hungary Liquidity Management Llc | Processo para a produção de um produto de fermentação |
WO2023028521A1 (fr) * | 2021-08-24 | 2023-03-02 | Inscripta, Inc. | Mutations de conception rationnelle à l'échelle du génome conduisant à une production améliorée de cellobiohydrolase i dans s. cerevisiae |
GB202115470D0 (en) * | 2021-10-27 | 2021-12-08 | Univ Stellenbosch | Recombinant saccharomyces cerevisiae strains for enzymatic hydrolysis of bioplastic polymers |
Family Cites Families (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
ES2076544T3 (es) | 1990-08-28 | 1995-11-01 | Du Pont | Procedimiento para seleccion rapida de vectores de secrecion eficaces. |
CA2136178A1 (fr) | 1992-05-29 | 1993-12-09 | Vasantha Nagarajan | Production de straptavidine a partir bacillus subtilis |
WO2003000941A2 (fr) * | 2001-06-26 | 2003-01-03 | Novozymes A/S | Polypeptides presentant une activite de cellobiohydrolase i et polynucleotides codant pour ceux-ci |
US7459299B2 (en) * | 2003-04-01 | 2008-12-02 | Danisco A/S, Genencor Division | Variant Humicola grisea CBH1.1 |
US8097445B2 (en) | 2004-03-25 | 2012-01-17 | Danisco Us Inc. | Exo-endo cellulase fusion protein |
EP2420570B1 (fr) * | 2006-02-10 | 2013-12-04 | Verenium Corporation | Enzyme Arabinofuranosidases, acides nucléiques les codant et leurs procédés de fabrication et d'utilisation |
EP2099923A2 (fr) | 2006-11-22 | 2009-09-16 | The Trustees of Dartmouth College | Souches de levures recombinées exprimant des enzymes de type cellulase << attachée >> |
BRPI0911966B1 (pt) | 2008-05-11 | 2018-11-13 | Univ Stellenbosch | polinucleotídeo codificando uma celobioidrolase |
WO2010005553A1 (fr) * | 2008-07-07 | 2010-01-14 | Mascoma Corporation | Isolement et caractérisation de cellobiohydrolase i (cbh 1) de schizochytrium aggregatum |
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2009
- 2009-05-11 BR BRPI0911966A patent/BRPI0911966B1/pt active IP Right Grant
- 2009-05-11 CA CA3021166A patent/CA3021166C/fr active Active
- 2009-05-11 WO PCT/IB2009/005881 patent/WO2009138877A2/fr active Application Filing
- 2009-05-11 US US12/992,003 patent/US9365842B2/en active Active
- 2009-05-11 CA CA2724076A patent/CA2724076C/fr active Active
-
2016
- 2016-05-25 US US15/164,258 patent/US11530428B2/en active Active
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US11530428B2 (en) | 2008-05-11 | 2022-12-20 | Stellenbosch University | Nucleic acids encoding fungal cellobiohydrolases for expression in yeast |
Also Published As
Publication number | Publication date |
---|---|
US20160326560A1 (en) | 2016-11-10 |
BRPI0911966A2 (pt) | 2015-07-28 |
US11530428B2 (en) | 2022-12-20 |
CA3021166C (fr) | 2024-01-09 |
CA3021166A1 (fr) | 2009-11-19 |
CA2724076A1 (fr) | 2009-11-19 |
US9365842B2 (en) | 2016-06-14 |
BRPI0911966B1 (pt) | 2018-11-13 |
WO2009138877A2 (fr) | 2009-11-19 |
WO2009138877A3 (fr) | 2010-01-07 |
US20110124074A1 (en) | 2011-05-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request |
Effective date: 20140507 |