CA2714353A1 - Process for the preparation of phosphorothioate oligonucleotides - Google Patents

Process for the preparation of phosphorothioate oligonucleotides Download PDF

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Publication number
CA2714353A1
CA2714353A1 CA2714353A CA2714353A CA2714353A1 CA 2714353 A1 CA2714353 A1 CA 2714353A1 CA 2714353 A CA2714353 A CA 2714353A CA 2714353 A CA2714353 A CA 2714353A CA 2714353 A1 CA2714353 A1 CA 2714353A1
Authority
CA
Canada
Prior art keywords
oligonucleotide
solid support
process according
acetonitrile
cleaving
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2714353A
Other languages
English (en)
French (fr)
Inventor
Mark Edward Douglas
Ben James Mellor
Donald Alfred Wellings
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nitto Denko Avecia Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0029610A external-priority patent/GB0029610D0/en
Priority claimed from US09/740,031 external-priority patent/US6768005B2/en
Application filed by Individual filed Critical Individual
Publication of CA2714353A1 publication Critical patent/CA2714353A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
CA2714353A 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides Abandoned CA2714353A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0029610.3 2000-12-05
GB0029610A GB0029610D0 (en) 2000-12-05 2000-12-05 Process
US09/740,031 US6768005B2 (en) 2000-12-20 2000-12-20 Process
US09/740,031 2000-12-20
CA2437040A CA2437040C (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2437040A Division CA2437040C (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides

Publications (1)

Publication Number Publication Date
CA2714353A1 true CA2714353A1 (en) 2002-06-13

Family

ID=26245377

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2714353A Abandoned CA2714353A1 (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides
CA2437040A Expired - Lifetime CA2437040C (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2437040A Expired - Lifetime CA2437040C (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides

Country Status (8)

Country Link
US (1) US7227017B2 (https=)
EP (2) EP2332954A3 (https=)
JP (1) JP2004517089A (https=)
KR (1) KR20040016826A (https=)
CN (1) CN1302005C (https=)
AU (1) AU2002220870A1 (https=)
CA (2) CA2714353A1 (https=)
WO (1) WO2002046205A2 (https=)

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US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3350314A4 (en) 2015-09-18 2019-02-06 Twist Bioscience Corporation OLIGONUCLEIC ACID VARIANT LIBRARIES VARIANT AND SYNTHESIS THEREOF
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
EP3500672A4 (en) 2016-08-22 2020-05-20 Twist Bioscience Corporation DE NOVO SYNTHETIZED NUCLEIC ACID LIBRARIES
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
EP4556433A3 (en) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
CN109956987B (zh) * 2017-12-14 2022-05-17 南京金斯瑞生物科技有限公司 氨解方法及氨解组合物
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
WO2020139871A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
JP2022548783A (ja) 2019-09-23 2022-11-21 ツイスト バイオサイエンス コーポレーション 単一ドメイン抗体のバリアント核酸ライブラリー
EP4034566A4 (en) 2019-09-23 2024-01-24 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CRTH2
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
DK4137500T3 (da) 2020-04-14 2026-04-20 Sumitomo Chemical Co Fremgangsmåde til fremstilling af nukleinsyreoligomer
KR20230119197A (ko) * 2020-12-18 2023-08-16 일라이 릴리 앤드 캄파니 진동 유동층 올리고뉴클레오티드 합성기
CN114573652B (zh) * 2022-03-15 2025-02-07 安徽瑞拜药业有限公司 药用核酸合成工艺
CN116854754B (zh) 2023-09-01 2023-12-12 北京悦康科创医药科技股份有限公司 一种含有核糖环或其衍生结构的GalNAc化合物及其寡核苷酸缀合物

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US4959463A (en) * 1985-10-15 1990-09-25 Genentech, Inc. Intermediates
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US4965349A (en) * 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
US5216141A (en) * 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
JPH0291088A (ja) * 1988-09-29 1990-03-30 Central Glass Co Ltd ホスファイトおよびヌクレオシド‐３’‐ホスファイト誘導体およびこれを用いるオリゴヌクレオチドの合成法
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US5164491A (en) 1989-06-15 1992-11-17 Gilead Sciences Large scale synthesis of oligonucleotides and their associated analogs
SE9003743D0 (sv) * 1990-11-26 1990-11-26 Pharmacia Ab Method and means for oligonucleotide synthesis
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WO1994001446A2 (en) * 1992-07-09 1994-01-20 Beckman Instruments, Inc. Derivatized organic solid support for nucleic acid synthesis
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US6465628B1 (en) * 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
US6887990B1 (en) 1999-02-05 2005-05-03 Amersham Biosciences Corp Method for deprotecting oligonucleotides
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MXPA02012216A (es) * 2000-06-12 2004-08-19 Avecia Biotechnology Inc Desproteccion de oligonucleotidos sinteticos usando un secuestrante de acrilonitrilo.
US6768005B2 (en) * 2000-12-20 2004-07-27 Avecia Limited Process
JP4348044B2 (ja) * 2002-02-12 2009-10-21 株式会社キラルジェン 立体規則性の高いジヌクレオシドホスホロチオエートの製造法
GB0209539D0 (en) * 2002-04-26 2002-06-05 Avecia Ltd Monomer Polymer and process

Also Published As

Publication number Publication date
CA2437040C (en) 2011-01-25
KR20040016826A (ko) 2004-02-25
EP1343802B1 (en) 2012-05-30
JP2004517089A (ja) 2004-06-10
EP2332954A2 (en) 2011-06-15
EP2332954A3 (en) 2011-12-21
CN1302005C (zh) 2007-02-28
US20030229221A1 (en) 2003-12-11
CA2437040A1 (en) 2002-06-13
EP1343802A2 (en) 2003-09-17
US7227017B2 (en) 2007-06-05
CN1479745A (zh) 2004-03-03
WO2002046205A3 (en) 2002-08-15
AU2002220870A1 (en) 2002-06-18
WO2002046205A2 (en) 2002-06-13

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued

Effective date: 20141107