CA2714353A1 - Process for the preparation of phosphorothioate oligonucleotides - Google Patents

Process for the preparation of phosphorothioate oligonucleotides Download PDF

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Publication number
CA2714353A1
CA2714353A1 CA2714353A CA2714353A CA2714353A1 CA 2714353 A1 CA2714353 A1 CA 2714353A1 CA 2714353 A CA2714353 A CA 2714353A CA 2714353 A CA2714353 A CA 2714353A CA 2714353 A1 CA2714353 A1 CA 2714353A1
Authority
CA
Canada
Prior art keywords
oligonucleotide
solid support
process according
acetonitrile
cleaving
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2714353A
Other languages
English (en)
French (fr)
Inventor
Mark Edward Douglas
Ben James Mellor
Donald Alfred Wellings
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nitto Denko Avecia Inc
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB0029610A external-priority patent/GB0029610D0/en
Priority claimed from US09/740,031 external-priority patent/US6768005B2/en
Application filed by Individual filed Critical Individual
Publication of CA2714353A1 publication Critical patent/CA2714353A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Saccharide Compounds (AREA)
CA2714353A 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides Abandoned CA2714353A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
GB0029610.3 2000-05-12
GB0029610A GB0029610D0 (en) 2000-12-05 2000-12-05 Process
US09/740,031 2000-12-20
US09/740,031 US6768005B2 (en) 2000-12-20 2000-12-20 Process
CA2437040A CA2437040C (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2437040A Division CA2437040C (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides

Publications (1)

Publication Number Publication Date
CA2714353A1 true CA2714353A1 (en) 2002-06-13

Family

ID=26245377

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2714353A Abandoned CA2714353A1 (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides
CA2437040A Expired - Lifetime CA2437040C (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2437040A Expired - Lifetime CA2437040C (en) 2000-12-05 2001-12-03 Process for the preparation of phosphorothioate oligonucleotides

Country Status (8)

Country Link
US (1) US7227017B2 (https=)
EP (2) EP2332954A3 (https=)
JP (1) JP2004517089A (https=)
KR (1) KR20040016826A (https=)
CN (1) CN1302005C (https=)
AU (1) AU2002220870A1 (https=)
CA (2) CA2714353A1 (https=)
WO (1) WO2002046205A2 (https=)

Families Citing this family (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0229443D0 (en) * 2002-12-18 2003-01-22 Avecia Ltd Process
EP1660512A4 (en) * 2003-06-02 2009-12-23 Isis Pharmaceuticals Inc SYNTHESIS OF OLIGONUCLEOTIDES USING ALTERNATIVE SOLVENTS
JP5956108B2 (ja) * 2007-10-15 2016-07-20 ハネウェル・インターナショナル・インコーポレーテッド １，３，５−トリメチルヘキサヒドロ−１，３，５−トリアジンを含有しないｎ−メチルイミダゾールのキャッピング剤を用いたオリゴヌクレオチドまたはホスホロチオ酸オリゴヌクレオチドの合成
WO2012059510A1 (en) * 2010-11-02 2012-05-10 Girindus America, Inc. Back pressure control during solid-phase synthesis on polymeric supports
EP2885312B1 (en) * 2012-08-15 2025-09-03 Ionis Pharmaceuticals, Inc. Method of preparing oligomeric compounds using modified capping protocols
TWI707038B (zh) 2013-08-05 2020-10-11 美商扭轉生物科技有限公司 重新合成之基因庫
WO2016126882A1 (en) 2015-02-04 2016-08-11 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
KR20180050411A (ko) 2015-09-18 2018-05-14 트위스트 바이오사이언스 코포레이션 올리고핵산 변이체 라이브러리 및 그의 합성
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
JP6854340B2 (ja) 2016-08-22 2021-04-07 ツイスト バイオサイエンス コーポレーション デノボ合成された核酸ライブラリ
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
KR102514213B1 (ko) 2016-12-16 2023-03-27 트위스트 바이오사이언스 코포레이션 면역 시냅스의 변이체 라이브러리 및 그의 합성
CN110892485B (zh) 2017-02-22 2024-03-22 特韦斯特生物科学公司 基于核酸的数据存储
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
US10696965B2 (en) 2017-06-12 2020-06-30 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CN111566125A (zh) 2017-09-11 2020-08-21 特韦斯特生物科学公司 Gpcr结合蛋白及其合成
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
CN109956987B (zh) * 2017-12-14 2022-05-17 南京金斯瑞生物科技有限公司 氨解方法及氨解组合物
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
US12357959B2 (en) 2018-12-26 2025-07-15 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
CA3131691A1 (en) 2019-02-26 2020-09-03 Twist Bioscience Corporation Variant nucleic acid libraries for antibody optimization
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
AU2020298294A1 (en) 2019-06-21 2022-02-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
WO2021061842A1 (en) 2019-09-23 2021-04-01 Twist Bioscience Corporation Variant nucleic acid libraries for single domain antibodies
JP2022548309A (ja) 2019-09-23 2022-11-17 ツイスト バイオサイエンス コーポレーション Ｃｒｔｈ２のバリアント核酸ライブラリー
IL293670A (en) 2019-12-09 2022-08-01 Twist Bioscience Corp Variable nucleic acid libraries for adenosine receptors
CN115362163B (zh) 2020-04-14 2025-04-11 住友化学株式会社 核酸寡聚物的制造方法
MX2023007242A (es) * 2020-12-18 2023-06-29 Lilly Co Eli Sintetizador de oligonucleotidos de lecho fluidizado oscilante.
CN114573652B (zh) * 2022-03-15 2025-02-07 安徽瑞拜药业有限公司 药用核酸合成工艺
CN116854754B (zh) 2023-09-01 2023-12-12 北京悦康科创医药科技股份有限公司 一种含有核糖环或其衍生结构的GalNAc化合物及其寡核苷酸缀合物

Family Cites Families (35)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5132418A (en) * 1980-02-29 1992-07-21 University Patents, Inc. Process for preparing polynucleotides
US4458066A (en) * 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4500707A (en) * 1980-02-29 1985-02-19 University Patents, Inc. Nucleosides useful in the preparation of polynucleotides
US4973679A (en) * 1981-03-27 1990-11-27 University Patents, Inc. Process for oligonucleo tide synthesis using phosphormidite intermediates
CA1206457A (en) * 1981-10-07 1986-06-24 Alan Rosevear Synthesis of compounds
USRE34069E (en) * 1983-08-18 1992-09-15 Biosyntech Gmbh Process for the preparation of oligonucleotides
US5362866A (en) 1983-09-02 1994-11-08 Molecular Biosystems, Inc. Oligonucleotide polymeric support system with an oxidation cleavable link
US4795700A (en) 1985-01-25 1989-01-03 California Institute Of Technology Nucleic acid probes and methods of using same
EP0216357A3 (en) * 1985-09-25 1988-08-31 Nippon Zeon Co., Ltd. Phosphoramidite compounds and process for production thereof
US4959463A (en) * 1985-10-15 1990-09-25 Genentech, Inc. Intermediates
CA1315968C (en) * 1987-04-24 1993-04-13 David Colin Sherrington Substrate and process for making a substrate
US4965349A (en) * 1987-12-24 1990-10-23 Applied Biosystems, Inc. Method of synthesizing oligonucleotides labeled with ammonia-labile groups on solid phase supports
US5216141A (en) * 1988-06-06 1993-06-01 Benner Steven A Oligonucleotide analogs containing sulfur linkages
JPH0291088A (ja) * 1988-09-29 1990-03-30 Central Glass Co Ltd ホスファイトおよびヌクレオシド‐３’‐ホスファイト誘導体およびこれを用いるオリゴヌクレオチドの合成法
US5047524A (en) 1988-12-21 1991-09-10 Applied Biosystems, Inc. Automated system for polynucleotide synthesis and purification
US5164491A (en) 1989-06-15 1992-11-17 Gilead Sciences Large scale synthesis of oligonucleotides and their associated analogs
SE9003743D0 (sv) * 1990-11-26 1990-11-26 Pharmacia Ab Method and means for oligonucleotide synthesis
EP0625214A4 (en) 1991-12-23 1997-07-16 Chiron Corp Cmv probes for use in solution phase sandwich hybridization assays.
WO1994001446A2 (en) * 1992-07-09 1994-01-20 Beckman Instruments, Inc. Derivatized organic solid support for nucleic acid synthesis
US5614621A (en) * 1993-07-29 1997-03-25 Isis Pharmaceuticals, Inc. Process for preparing oligonucleotides using silyl-containing diamino phosphorous reagents
US5514789A (en) 1994-04-21 1996-05-07 Barrskogen, Inc. Recovery of oligonucleotides by gas phase cleavage
US5554746A (en) * 1994-05-16 1996-09-10 Isis Pharmaceuticals, Inc. Lactam nucleic acids
US5510476A (en) * 1994-07-07 1996-04-23 Isis Pharmaceuticals, Inc. Carbocation scavenging during oligonucleotide synthesis
US5705621A (en) * 1995-11-17 1998-01-06 Isis Pharmaceuticals, Inc. Oligomeric phosphite, phosphodiester, Phosphorothioate and phosphorodithioate compounds and intermediates for preparing same
US5869696A (en) * 1996-04-22 1999-02-09 Beckman Instruments, Inc. Universal solid supports and methods for their use
US6096881A (en) * 1997-05-30 2000-08-01 Hybridon, Inc. Sulfur transfer reagents for oligonucleotide synthesis
US6111086A (en) * 1998-02-27 2000-08-29 Scaringe; Stephen A. Orthoester protecting groups
US6069243A (en) * 1998-10-06 2000-05-30 Isis Pharmaceuticals, Inc. Process for oligonucleotide synthesis
US6465628B1 (en) * 1999-02-04 2002-10-15 Isis Pharmaceuticals, Inc. Process for the synthesis of oligomeric compounds
DK1149109T3 (da) 1999-02-05 2010-01-11 Ge Healthcare Bio Sciences Fremgangsmåde til afbeskyttelse af oligonukleotider
GB9924285D0 (en) * 1999-10-14 1999-12-15 Avecia Ltd Process
MXPA02012216A (es) * 2000-06-12 2004-08-19 Avecia Biotechnology Inc Desproteccion de oligonucleotidos sinteticos usando un secuestrante de acrilonitrilo.
US6768005B2 (en) * 2000-12-20 2004-07-27 Avecia Limited Process
JP4348044B2 (ja) * 2002-02-12 2009-10-21 株式会社キラルジェン 立体規則性の高いジヌクレオシドホスホロチオエートの製造法
GB0209539D0 (en) * 2002-04-26 2002-06-05 Avecia Ltd Monomer Polymer and process

Also Published As

Publication number Publication date
EP1343802B1 (en) 2012-05-30
EP2332954A2 (en) 2011-06-15
CN1479745A (zh) 2004-03-03
CN1302005C (zh) 2007-02-28
EP2332954A3 (en) 2011-12-21
CA2437040C (en) 2011-01-25
AU2002220870A1 (en) 2002-06-18
KR20040016826A (ko) 2004-02-25
JP2004517089A (ja) 2004-06-10
WO2002046205A2 (en) 2002-06-13
CA2437040A1 (en) 2002-06-13
US20030229221A1 (en) 2003-12-11
US7227017B2 (en) 2007-06-05
WO2002046205A3 (en) 2002-08-15
EP1343802A2 (en) 2003-09-17

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued

Effective date: 20141107