CA2710359C - Yeast organism producing isobutanol at a high yield - Google Patents
Yeast organism producing isobutanol at a high yield Download PDFInfo
- Publication number
- CA2710359C CA2710359C CA2710359A CA2710359A CA2710359C CA 2710359 C CA2710359 C CA 2710359C CA 2710359 A CA2710359 A CA 2710359A CA 2710359 A CA2710359 A CA 2710359A CA 2710359 C CA2710359 C CA 2710359C
- Authority
- CA
- Canada
- Prior art keywords
- isobutanol
- microorganism
- yeast
- pdc
- recombinant yeast
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/88—Lyases (4.)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
- C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
- C12P7/16—Butanols
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y401/00—Carbon-carbon lyases (4.1)
- C12Y401/01—Carboxy-lyases (4.1.1)
- C12Y401/01001—Pyruvate decarboxylase (4.1.1.1)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CA2990939A CA2990939A1 (en) | 2007-12-23 | 2008-12-23 | Yeast organism producing isobutanol at a high yield |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US1648307P | 2007-12-23 | 2007-12-23 | |
| US61/016,483 | 2007-12-23 | ||
| PCT/US2008/088235 WO2009086423A2 (en) | 2007-12-23 | 2008-12-23 | Yeast organism producing isobutanol at a high yield |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2990939A Division CA2990939A1 (en) | 2007-12-23 | 2008-12-23 | Yeast organism producing isobutanol at a high yield |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2710359A1 CA2710359A1 (en) | 2009-07-09 |
| CA2710359C true CA2710359C (en) | 2018-02-20 |
Family
ID=40823160
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2710359A Active CA2710359C (en) | 2007-12-23 | 2008-12-23 | Yeast organism producing isobutanol at a high yield |
| CA2990939A Abandoned CA2990939A1 (en) | 2007-12-23 | 2008-12-23 | Yeast organism producing isobutanol at a high yield |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2990939A Abandoned CA2990939A1 (en) | 2007-12-23 | 2008-12-23 | Yeast organism producing isobutanol at a high yield |
Country Status (8)
| Country | Link |
|---|---|
| US (3) | US8017375B2 (OSRAM) |
| EP (1) | EP2235193B1 (OSRAM) |
| JP (1) | JP2011507532A (OSRAM) |
| CN (1) | CN101952448A (OSRAM) |
| CA (2) | CA2710359C (OSRAM) |
| DK (1) | DK2235193T3 (OSRAM) |
| ES (1) | ES2563040T3 (OSRAM) |
| WO (1) | WO2009086423A2 (OSRAM) |
Families Citing this family (70)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8273558B2 (en) | 2005-10-26 | 2012-09-25 | Butamax(Tm) Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| US9303225B2 (en) | 2005-10-26 | 2016-04-05 | Butamax Advanced Biofuels Llc | Method for the production of isobutanol by recombinant yeast |
| NZ566406A (en) | 2005-10-26 | 2012-04-27 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| US8945899B2 (en) | 2007-12-20 | 2015-02-03 | Butamax Advanced Biofuels Llc | Ketol-acid reductoisomerase using NADH |
| US8956850B2 (en) * | 2008-06-05 | 2015-02-17 | Butamax Advanced Biofuels Llc | Enhanced pyruvate to acetolactate conversion in yeast |
| US9186593B2 (en) | 2006-06-07 | 2015-11-17 | Toray Plastics (America), Inc. | Stretchable and formable lighter than air balloons made from a biaxially oriented polyester film |
| US8399080B2 (en) | 2006-06-07 | 2013-03-19 | Toray Plastics (America), Inc. | Lighter than air balloon made from a biaxially oriented polyester film |
| US20090111154A1 (en) * | 2007-04-04 | 2009-04-30 | The Regents Of The University Of California | Butanol production by recombinant microorganisms |
| WO2008130995A2 (en) | 2007-04-18 | 2008-10-30 | E. I. Du Pont De Nemours And Company | Fermentive production of isobutanol using highly active ketol-acid reductoisomerase enzymes |
| WO2009059254A2 (en) * | 2007-10-31 | 2009-05-07 | Gevo, Inc | Methods for the economical production of biofuel precursor that is also a biofuel from biomass |
| EP2225351A4 (en) | 2007-12-03 | 2016-11-09 | Gevo Inc | RENEWABLE COMPOSITIONS |
| CN101952448A (zh) * | 2007-12-23 | 2011-01-19 | 格沃股份有限公司 | 高产率产生异丁醇的酵母生物 |
| US8455239B2 (en) | 2007-12-23 | 2013-06-04 | Gevo, Inc. | Yeast organism producing isobutanol at a high yield |
| BRPI0821519A2 (pt) | 2007-12-27 | 2015-06-16 | Gevo Inc | Recuperação de alcoois superiores a partir de soluções aquosas diluída |
| MX2011003313A (es) | 2008-09-29 | 2011-06-16 | Butamax Tm Advanced Biofuels | Actividad enzimatica de fe-s heterologa aumentada en levadura. |
| EP2821484B1 (en) | 2008-09-29 | 2016-06-29 | Butamax Advanced Biofuels LLC | Identification and use of Bacterial [2Fe-2S] dihydroxy-acid dehydratases |
| BRPI0921734A2 (pt) | 2008-10-31 | 2019-01-08 | California Inst Of Techn | micro-organismos criados geneticamente capazes de produzir compostos-alvo sob condições anaeróbicas |
| EP2464736A4 (en) * | 2009-08-12 | 2013-07-31 | Gevo Inc | PATIENT LOCALIZATION OF CYTOSOLIC ISOBUTANOL FOR THE MANUFACTURE OF ISOBUTANOL |
| KR20120051705A (ko) * | 2009-08-21 | 2012-05-22 | 아사히 가라스 가부시키가이샤 | 형질 전환체 및 그의 제조 방법, 및 락트산의 제조 방법 |
| JP5805094B2 (ja) * | 2009-09-29 | 2015-11-04 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | 高有効性のケトール酸レダクトイソメラーゼ酵素を用いたイソブタノールの発酵生産 |
| US9260708B2 (en) | 2009-09-29 | 2016-02-16 | Butamax Advanced Biofuels Llc | Yeast production host cells |
| GB2487889B (en) | 2009-11-24 | 2012-12-19 | Gevo Inc | Methods of increasing dihydroxy acid dehydratase activity to improve production of fuels, chemicals, and amino acids |
| KR20120099509A (ko) * | 2009-12-29 | 2012-09-10 | 부타맥스 어드밴스드 바이오퓨얼스 엘엘씨 | 재조합 숙주 세포에서 육탄당 키나아제의 발현 |
| CN102762722B (zh) | 2009-12-29 | 2015-05-20 | 布特马斯先进生物燃料有限责任公司 | 用于发酵生产低级烷基醇的醇脱氢酶(adh) |
| BR112012016883A2 (pt) | 2010-01-08 | 2018-06-05 | Gevo Inc | metodos integrados de preparar produsot quimicos renovaveis |
| CN102869763A (zh) * | 2010-02-12 | 2013-01-09 | 格沃股份有限公司 | 用于提高燃料、化学品和氨基酸产生的副产物蓄积减少的酵母微生物 |
| WO2011103300A2 (en) | 2010-02-17 | 2011-08-25 | Butamax(Tm) Advanced Biofuels Llc | Improving activity of fe-s cluster requiring proteins |
| AU2011228079B2 (en) | 2010-03-17 | 2015-06-25 | Toray Industries, Inc. | Butanol manufacturing method |
| US8373012B2 (en) | 2010-05-07 | 2013-02-12 | Gevo, Inc. | Renewable jet fuel blendstock from isobutanol |
| US9249420B2 (en) | 2010-05-31 | 2016-02-02 | Vib Vzw | Isobutanol production using yeasts with modified transporter expression |
| FR2961202B1 (fr) | 2010-06-11 | 2012-07-27 | Inst Francais Du Petrole | Procede de deshydratation et d'isomerisation d'alcools en c4 utilisant un solide amorphe a porosite adaptee |
| EP2582823A1 (en) * | 2010-06-18 | 2013-04-24 | Butamax(tm) Advanced Biofuels LLC | Supplementation of fatty acids for improving alcohol productivity |
| EP4242320A3 (en) | 2010-07-12 | 2023-11-29 | Inbiose N.V. | Metabolically engineered organisms for the production of added value bio-products |
| US20120088271A1 (en) * | 2010-09-30 | 2012-04-12 | Codexis, Inc. | Use of an Endogenous 2-Micron Yeast Plasmid for Gene Over Expression |
| EP2686432B1 (en) | 2011-03-14 | 2018-12-26 | Easel Biotechnologies, LLC | Microbial syntheses of aldehydes and corresponding alcohols |
| EP2689014B1 (en) | 2011-03-24 | 2019-04-24 | Butamax (TM) Advanced Biofuels LLC | Host cells and methods for production of isobutanol |
| EP2699618A4 (en) | 2011-04-19 | 2014-12-17 | Gevo Inc | VARIATIONS OF A PRINS REACTION FOR THE PREPARATION OF 2,5-DIMETHYLHEXADIENE FROM ISOBUTANOL |
| CN102816706B (zh) * | 2011-05-12 | 2013-08-21 | 江南大学 | 中国酱香型白酒生产中高产乙醇低产杂醇油的酵母cgmcc 4745的筛选与应用 |
| BR112013032320A2 (pt) * | 2011-06-17 | 2016-12-20 | Butamax Advanced Biofuels Llc | método e composição de produção de butanol, composição de butanol e isobutanol, método de cultivo de um micro-organismo produtor de butanol em material lignocelulósico e método para inibir o crescimento de micro-organismos que não produzem butanol em uma composição |
| US10137625B2 (en) | 2011-07-08 | 2018-11-27 | Toray Plastics (America), Inc. | Biaxially oriented bio-based polyester films and laminates |
| US9561676B2 (en) | 2011-07-08 | 2017-02-07 | Toray Plastics (America), Inc. | Biaxially oriented bio-based polyester thin films and laminates for thermal transfer printing |
| US9988648B2 (en) | 2011-09-16 | 2018-06-05 | Genomatica, Inc. | Microorganisms and methods for producing alkenes |
| WO2014039060A1 (en) * | 2011-10-07 | 2014-03-13 | Gevo, Inc. | Acetolactate synthases for improved metabolite production |
| EP2583957A1 (de) | 2011-10-18 | 2013-04-24 | LANXESS Deutschland GmbH | Lineare Butene aus Isobutanol |
| US9777297B2 (en) | 2011-11-03 | 2017-10-03 | Easel Biotechnologies, Llc | Microbial production of n-butyraldehyde |
| AU2013266727A1 (en) | 2012-05-11 | 2014-11-06 | Butamax Advanced Biofuels Llc | Ketol-acid reductoisomerase enzymes and methods of use |
| WO2014004616A2 (en) * | 2012-06-26 | 2014-01-03 | Gevo, Inc. | Engineered yeast with improved growth under low aeration |
| JP6407869B2 (ja) | 2012-09-26 | 2018-10-17 | ビュータマックス・アドバンスド・バイオフューエルズ・エルエルシー | ケトール酸レダクトイソメラーゼ活性を有するポリペプチド |
| WO2014106107A2 (en) | 2012-12-28 | 2014-07-03 | Butamax (Tm) Advanced Biofuels Llc | Dhad variants for butanol production |
| US9469860B2 (en) | 2013-01-18 | 2016-10-18 | Synata Bio, Inc. | Method for production of n-butanol from syngas using syntrophic co-cultures of anaerobic microorganisms |
| US9267158B2 (en) | 2013-03-14 | 2016-02-23 | Intrexon Corporation | Biological production of multi-carbon compounds from methane |
| WO2014144210A2 (en) * | 2013-03-15 | 2014-09-18 | Butamax Advanced Biofuels Llc | Competitive growth and/or production advantage for butanologen microorganism |
| US9580705B2 (en) | 2013-03-15 | 2017-02-28 | Butamax Advanced Biofuels Llc | DHAD variants and methods of screening |
| WO2015002913A1 (en) | 2013-07-03 | 2015-01-08 | Butamax Advanced Biofuels Llc | Partial adaptation for butanol production |
| CN103695252A (zh) * | 2014-01-14 | 2014-04-02 | 黑龙江大学 | 一种酵母液体曲的制备方法 |
| US11293038B2 (en) | 2014-07-14 | 2022-04-05 | Librede Inc. | Production of cannabinoids in yeast |
| CA2990071C (en) | 2014-07-14 | 2023-01-24 | Librede Inc. | Production of cannabigerolic acid |
| CA2957920A1 (en) | 2014-08-11 | 2016-02-18 | Butamax Advanced Biofuels Llc | Yeast preparations and methods of making the same |
| US10544082B2 (en) | 2015-06-10 | 2020-01-28 | Ptt Global Chemical Public Company Limited | Method to produce acrylic acid with acetaldehyde as the main by-product |
| CN108431229A (zh) * | 2015-12-17 | 2018-08-21 | 嘉吉公司 | 糖转运蛋白修饰的酵母菌株和用于生物制品生产的方法 |
| US20170368807A1 (en) | 2016-06-28 | 2017-12-28 | Toray Plastics (America), Inc. | Formable polyester films |
| BR112019002238A2 (pt) | 2016-08-05 | 2019-05-14 | Cargill, Incorporated | polipeptídeo e célula manipulados, e, método de fermentação. |
| WO2019036202A1 (en) * | 2017-08-17 | 2019-02-21 | Cargill, Incorporated | ISSATCHENKIA ORIENTALIS HAPLOID GENETICALLY MODIFIED |
| WO2019191263A1 (en) * | 2018-03-27 | 2019-10-03 | Cargill, Incorporated | Methods for ethanol production using engineered yeast |
| US10808265B2 (en) | 2018-09-26 | 2020-10-20 | Nantbio, Inc. | Microbes and methods for improved conversion of a feedstock |
| CN110106098B (zh) * | 2019-04-30 | 2022-05-20 | 广西科学院 | 一种高产丙酮酸的酿酒酵母工程菌株及其发酵方法 |
| WO2021163148A1 (en) * | 2020-02-11 | 2021-08-19 | Locus Ip Company, Llc | Methods and compositions for reducing deleterious enteric atmospheric gases in livestock |
| FR3115988A1 (fr) | 2020-11-06 | 2022-05-13 | Global Bioenergies | Composition de maquillage à base d’isododécane biosourcé et son procédé de préparation |
| FR3157389A1 (fr) | 2023-12-22 | 2025-06-27 | Axens | Procédé de production de bioalkylat ou d’essence biosourcée |
| WO2025226576A1 (en) | 2024-04-25 | 2025-10-30 | ExxonMobil Technology and Engineering Company | Processes for producing isobutylene and isobutylene-based polymers |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT1294728B1 (it) | 1997-09-12 | 1999-04-12 | Biopolo S C A R L | Ceppi di lievito per la riproduzione di acido lattico |
| US6660507B2 (en) * | 2000-09-01 | 2003-12-09 | E. I. Du Pont De Nemours And Company | Genes involved in isoprenoid compound production |
| CN1249239C (zh) * | 2000-11-22 | 2006-04-05 | 加吉尔·道聚合物公司 | 合成有机产物的方法和材料 |
| BR0311453A (pt) | 2002-05-30 | 2005-03-29 | Cargill Dow Llc | Métodos e materiais para produção de ácido d-láctico em levedura |
| EP1660667A4 (en) * | 2002-07-03 | 2011-01-05 | Basf Se | MICRO-ORGANISMS AND PROCESSES FOR IMPROVED PRODUCTION OF PANTOTHENATE |
| US7405068B2 (en) | 2003-05-02 | 2008-07-29 | Tate & Lyle Ingredients Americas, Inc. | Pyruvate producing yeast strain |
| US20060234264A1 (en) * | 2005-03-14 | 2006-10-19 | Affymetrix, Inc. | Multiplex polynucleotide synthesis |
| CA2602183C (en) | 2005-03-18 | 2014-05-06 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
| CA2611028A1 (en) | 2005-06-02 | 2007-03-22 | Cargill, Inc. | Genetically modified yeast of the species issatchenkia orientalis and closely related species and fermentation processes using same |
| US8956850B2 (en) * | 2008-06-05 | 2015-02-17 | Butamax Advanced Biofuels Llc | Enhanced pyruvate to acetolactate conversion in yeast |
| US8273558B2 (en) * | 2005-10-26 | 2012-09-25 | Butamax(Tm) Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| NZ566406A (en) * | 2005-10-26 | 2012-04-27 | Butamax Advanced Biofuels Llc | Fermentive production of four carbon alcohols |
| US20080090273A1 (en) | 2005-11-21 | 2008-04-17 | Aaron Adriaan Winkler | Malic Acid Production in Recombinant Yeast |
| EP2078092A2 (en) | 2006-09-28 | 2009-07-15 | Microbia, Inc. | Production of carotenoids in oleaginous yeast and fungi |
| EP2074214A2 (en) | 2006-09-28 | 2009-07-01 | Microbia, Inc. | Production of sterols in oleaginous yeast and fungi |
| US20100036174A1 (en) | 2006-10-31 | 2010-02-11 | Lourina Madeleine Raamsdonk | Butanol production in a eukaryotic cell |
| WO2008063650A2 (en) | 2006-11-20 | 2008-05-29 | President And Fellows Of Harvard College | Systems of hydrogen and formic acid production in yeast |
| CA2715092A1 (en) | 2006-12-21 | 2008-07-03 | Gevo, Inc. | Butanol production by metabolically engineered yeast |
| US20100226933A1 (en) | 2007-02-14 | 2010-09-09 | Universitaet Salzburg | Hypoallergenic proteins |
| JP4126379B1 (ja) * | 2007-02-15 | 2008-07-30 | 国立大学法人 岡山大学 | 卵認識フェロモンとしてリゾチーム、その塩、その生物学的フラグメントまたは関連ペプチドを用いる害虫駆除 |
| US20100159546A1 (en) | 2007-03-30 | 2010-06-24 | Aristos Aristidou | Metabolic engineering of yeasts for the production of 1-butanol |
| CN101952448A (zh) * | 2007-12-23 | 2011-01-19 | 格沃股份有限公司 | 高产率产生异丁醇的酵母生物 |
| US8455239B2 (en) * | 2007-12-23 | 2013-06-04 | Gevo, Inc. | Yeast organism producing isobutanol at a high yield |
| DE102008010121B4 (de) | 2008-02-20 | 2013-11-21 | Butalco Gmbh | Fermentative Produktion von Isobutanol mit Hefe |
| WO2009114357A2 (en) * | 2008-03-07 | 2009-09-17 | Gm Global Technology Operations, Inc. | Shape memory alloy cables |
| AU2009320163B2 (en) * | 2008-10-27 | 2014-10-02 | Butamax(Tm) Advanced Biofuels Llc | Carbon pathway optimized production hosts for the production of isobutanol |
-
2008
- 2008-12-23 CN CN2008801273441A patent/CN101952448A/zh active Pending
- 2008-12-23 US US12/343,375 patent/US8017375B2/en not_active Expired - Fee Related
- 2008-12-23 CA CA2710359A patent/CA2710359C/en active Active
- 2008-12-23 CA CA2990939A patent/CA2990939A1/en not_active Abandoned
- 2008-12-23 ES ES08867473.4T patent/ES2563040T3/es active Active
- 2008-12-23 EP EP08867473.4A patent/EP2235193B1/en active Active
- 2008-12-23 WO PCT/US2008/088235 patent/WO2009086423A2/en not_active Ceased
- 2008-12-23 DK DK08867473.4T patent/DK2235193T3/da active
- 2008-12-23 JP JP2010539945A patent/JP2011507532A/ja not_active Withdrawn
-
2010
- 2010-01-29 US US12/696,645 patent/US20110183392A1/en not_active Abandoned
-
2011
- 2011-09-09 US US13/229,438 patent/US20110318799A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP2235193B1 (en) | 2015-11-25 |
| US8017375B2 (en) | 2011-09-13 |
| JP2011507532A (ja) | 2011-03-10 |
| CA2710359A1 (en) | 2009-07-09 |
| US20110318799A1 (en) | 2011-12-29 |
| CA2990939A1 (en) | 2009-07-09 |
| DK2235193T3 (da) | 2016-02-29 |
| US20090226991A1 (en) | 2009-09-10 |
| WO2009086423A3 (en) | 2009-09-17 |
| US20110183392A1 (en) | 2011-07-28 |
| EP2235193A2 (en) | 2010-10-06 |
| WO2009086423A2 (en) | 2009-07-09 |
| ES2563040T3 (es) | 2016-03-10 |
| CN101952448A (zh) | 2011-01-19 |
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