CA2700945A1 - Nucleotide triphosphate avec marqueur electroactif conjugue au phosphate gamma - Google Patents
Nucleotide triphosphate avec marqueur electroactif conjugue au phosphate gamma Download PDFInfo
- Publication number
- CA2700945A1 CA2700945A1 CA2700945A CA2700945A CA2700945A1 CA 2700945 A1 CA2700945 A1 CA 2700945A1 CA 2700945 A CA2700945 A CA 2700945A CA 2700945 A CA2700945 A CA 2700945A CA 2700945 A1 CA2700945 A1 CA 2700945A1
- Authority
- CA
- Canada
- Prior art keywords
- kinase
- substrate
- phosphorylation
- electroactive
- gamma phosphate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/30—Electrochemically active labels
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US96038807P | 2007-09-27 | 2007-09-27 | |
US60/960,398 | 2007-09-27 | ||
PCT/CA2008/001690 WO2009039639A1 (fr) | 2007-09-27 | 2008-09-26 | Nucléotide triphosphate avec marqueur électroactif conjugué au phosphate gamma |
Publications (1)
Publication Number | Publication Date |
---|---|
CA2700945A1 true CA2700945A1 (fr) | 2009-04-02 |
Family
ID=40510705
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2700945A Abandoned CA2700945A1 (fr) | 2007-09-27 | 2008-09-26 | Nucleotide triphosphate avec marqueur electroactif conjugue au phosphate gamma |
Country Status (4)
Country | Link |
---|---|
EP (1) | EP2195447A4 (fr) |
CN (1) | CN102016064A (fr) |
CA (1) | CA2700945A1 (fr) |
WO (1) | WO2009039639A1 (fr) |
Families Citing this family (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010144150A2 (fr) | 2009-06-12 | 2010-12-16 | Pacific Biosciences Of California, Inc. | Procédés et systèmes analytiques en temps réel |
CN105548301B (zh) * | 2016-01-28 | 2018-07-31 | 中南大学 | 一种测定蛋白激酶活性的方法和蛋白激酶抑制剂活性的方法 |
CN109030583B (zh) * | 2018-08-28 | 2023-09-15 | 长沙理工大学 | 基于2-巯基苯并咪唑的l-半胱氨酸的检测方法及传感器 |
CN111693707A (zh) * | 2020-07-16 | 2020-09-22 | 首都医科大学附属北京朝阳医院 | Rock激酶活性在辅助诊断血管炎患者中的应用 |
CN114235922A (zh) * | 2020-09-09 | 2022-03-25 | 中国科学院大连化学物理研究所 | 一种利用功能纳米通道实时监测磷酸化的方法 |
CN113430248B (zh) * | 2021-06-28 | 2022-11-11 | 中国人民解放军军事科学院军事医学研究院 | 一种新型p53蛋白激酶的非同位素筛选方法及其应用 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2001006016A2 (fr) * | 1999-07-20 | 2001-01-25 | Clinical Micro Sensors, Inc. | Amplification d'acides nucleiques avec detection electronique |
WO2004090154A2 (fr) * | 2003-04-01 | 2004-10-21 | Activx Biosciences, Inc. | Sondes d'acyle-phosphate et de phosphonate et procedes pour les synthetiser et les utiliser dans le cadre d'une analyse proteomique |
-
2008
- 2008-09-26 CA CA2700945A patent/CA2700945A1/fr not_active Abandoned
- 2008-09-26 CN CN2008801094672A patent/CN102016064A/zh active Pending
- 2008-09-26 EP EP08800381A patent/EP2195447A4/fr not_active Withdrawn
- 2008-09-26 WO PCT/CA2008/001690 patent/WO2009039639A1/fr active Application Filing
Also Published As
Publication number | Publication date |
---|---|
WO2009039639A8 (fr) | 2009-05-07 |
EP2195447A4 (fr) | 2011-03-30 |
EP2195447A1 (fr) | 2010-06-16 |
WO2009039639A1 (fr) | 2009-04-02 |
CN102016064A (zh) | 2011-04-13 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Adeloju | Progress and recent advances in phosphate sensors: A review | |
Kerman et al. | Gold nanoparticle-based electrochemical detection of protein phosphorylation | |
Shults et al. | A multiplexed homogeneous fluorescence-based assay for protein kinase activity in cell lysates | |
US4942127A (en) | Polyredox couples in analyte determinations | |
Song et al. | Electrochemical detection of kinase-catalyzed phosphorylation using ferrocene-conjugated ATP | |
Jia et al. | Aptamer based electrochemical assay for protein kinase activity by coupling hybridization chain reaction | |
CA2700945A1 (fr) | Nucleotide triphosphate avec marqueur electroactif conjugue au phosphate gamma | |
Yin et al. | A sensitive electrochemical biosensor for detection of protein kinase A activity and inhibitors based on Phos-tag and enzymatic signal amplification | |
Wang et al. | Multiple signal amplification electrogenerated chemiluminescence biosensors for sensitive protein kinase activity analysis and inhibition | |
Kerman et al. | Electrochemical detection of protein tyrosine kinase-catalysed phosphorylation using gold nanoparticles | |
Yang et al. | Label-free electrochemical measurement of protein tyrosine kinase activity and inhibition based on electro-catalyzed tyrosine signaling | |
CN101558296A (zh) | 用于测定全血中的1,5-脱水葡萄糖醇的方法以及用于所述方法的传感器芯片和测定试剂盒 | |
Radi et al. | Electrochemical and spectral studies on the interaction of the antiparasitic drug nitazoxanide with DNA | |
US7169273B2 (en) | Enzyme electrode | |
Gonzalez et al. | Amperometric sensor for hypoxanthine and xanthine based on the detection of uric acid | |
CN109856212B (zh) | 一种同时检测HAT和TdT的电化学生物传感器的制备方法及其应用 | |
Wilson et al. | Hydroquinone diphosphate: an alkaline phosphatase substrate that does not produce electrode fouling in electrochemical immunoassays | |
Li et al. | Label-free electrochemical impedance detection of kinase and phosphatase activities using carbon nanofiber nanoelectrode arrays | |
Yin et al. | Electrochemical detection of protein kinase activity based on carboxypeptidase Y digestion triggered signal amplification | |
Yang et al. | Label-free electrochemical biosensing of small-molecule inhibition on O-GlcNAc glycosylation | |
Hacker et al. | Direct monitoring of nucleotide turnover in human cell extracts and cells by fluorogenic ATP analogs | |
WO2000055356A1 (fr) | Dosage fluorimetrique enzymatique de camp et de l'adenylate cyclase | |
JP2010531976A (ja) | 酸化還元メディエーター | |
Taylor et al. | PTEN and myotubularins: families of phosphoinositide phosphatases | |
Li et al. | An ultrasensitive and label-free electrochemical DNA biosensor for detection of DNase I activity |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
EEER | Examination request |
Effective date: 20130926 |
|
FZDE | Discontinued |
Effective date: 20150928 |