CA2680613A1 - Methods for generation of antibodies - Google Patents
Methods for generation of antibodies Download PDFInfo
- Publication number
- CA2680613A1 CA2680613A1 CA002680613A CA2680613A CA2680613A1 CA 2680613 A1 CA2680613 A1 CA 2680613A1 CA 002680613 A CA002680613 A CA 002680613A CA 2680613 A CA2680613 A CA 2680613A CA 2680613 A1 CA2680613 A1 CA 2680613A1
- Authority
- CA
- Canada
- Prior art keywords
- animal
- cell
- antigen
- myc
- antibody
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000000034 method Methods 0.000 title claims abstract description 108
- 239000000427 antigen Substances 0.000 claims abstract description 202
- 108091007433 antigens Proteins 0.000 claims abstract description 200
- 102000036639 antigens Human genes 0.000 claims abstract description 200
- 210000003719 b-lymphocyte Anatomy 0.000 claims abstract description 163
- 241001465754 Metazoa Species 0.000 claims abstract description 160
- 108700020978 Proto-Oncogene Proteins 0.000 claims abstract description 40
- 102000052575 Proto-Oncogene Human genes 0.000 claims abstract description 40
- 238000004519 manufacturing process Methods 0.000 claims abstract description 36
- 210000000628 antibody-producing cell Anatomy 0.000 claims abstract description 21
- 210000004027 cell Anatomy 0.000 claims description 141
- 210000001185 bone marrow Anatomy 0.000 claims description 59
- 108090000623 proteins and genes Proteins 0.000 claims description 51
- 150000007523 nucleic acids Chemical class 0.000 claims description 44
- 108020004707 nucleic acids Proteins 0.000 claims description 43
- 102000039446 nucleic acids Human genes 0.000 claims description 43
- 102000004169 proteins and genes Human genes 0.000 claims description 38
- 210000000130 stem cell Anatomy 0.000 claims description 37
- 239000012634 fragment Substances 0.000 claims description 25
- 229960003722 doxycycline Drugs 0.000 claims description 23
- 101710154606 Hemagglutinin Proteins 0.000 claims description 18
- 101710093908 Outer capsid protein VP4 Proteins 0.000 claims description 18
- 101710135467 Outer capsid protein sigma-1 Proteins 0.000 claims description 18
- 101710176177 Protein A56 Proteins 0.000 claims description 18
- 238000012258 culturing Methods 0.000 claims description 18
- 230000004083 survival effect Effects 0.000 claims description 16
- 230000014509 gene expression Effects 0.000 claims description 15
- 230000035755 proliferation Effects 0.000 claims description 15
- 239000000185 hemagglutinin Substances 0.000 claims description 13
- 238000004113 cell culture Methods 0.000 claims description 12
- 210000003958 hematopoietic stem cell Anatomy 0.000 claims description 12
- 238000001727 in vivo Methods 0.000 claims description 12
- 230000009261 transgenic effect Effects 0.000 claims description 11
- 230000003115 biocidal effect Effects 0.000 claims description 9
- 230000001404 mediated effect Effects 0.000 claims description 9
- 238000011579 SCID mouse model Methods 0.000 claims description 8
- 208000023275 Autoimmune disease Diseases 0.000 claims description 6
- 230000001363 autoimmune Effects 0.000 claims description 6
- 238000012239 gene modification Methods 0.000 claims description 6
- 230000005017 genetic modification Effects 0.000 claims description 6
- 235000013617 genetically modified food Nutrition 0.000 claims description 6
- 241000712461 unidentified influenza virus Species 0.000 claims description 6
- 230000006909 anti-apoptosis Effects 0.000 claims description 5
- 230000008021 deposition Effects 0.000 claims description 5
- 102100021569 Apoptosis regulator Bcl-2 Human genes 0.000 claims description 4
- 108020004414 DNA Proteins 0.000 claims description 4
- 101000971171 Homo sapiens Apoptosis regulator Bcl-2 Proteins 0.000 claims description 4
- 238000006243 chemical reaction Methods 0.000 claims description 4
- 230000007774 longterm Effects 0.000 claims description 4
- 239000003242 anti bacterial agent Substances 0.000 claims description 2
- 230000002463 transducing effect Effects 0.000 claims description 2
- 101800001690 Transmembrane protein gp41 Proteins 0.000 claims 1
- XQTWDDCIUJNLTR-CVHRZJFOSA-N doxycycline monohydrate Chemical compound O.O=C1C2=C(O)C=CC=C2[C@H](C)[C@@H]2C1=C(O)[C@]1(O)C(=O)C(C(N)=O)=C(O)[C@@H](N(C)C)[C@@H]1[C@H]2O XQTWDDCIUJNLTR-CVHRZJFOSA-N 0.000 claims 1
- 206010035226 Plasma cell myeloma Diseases 0.000 abstract description 15
- 201000000050 myeloid neoplasm Diseases 0.000 abstract description 14
- 230000004927 fusion Effects 0.000 abstract description 12
- 230000001900 immune effect Effects 0.000 abstract description 5
- 241000699670 Mus sp. Species 0.000 description 121
- 108060003951 Immunoglobulin Proteins 0.000 description 38
- 102000018358 immunoglobulin Human genes 0.000 description 38
- 206010028980 Neoplasm Diseases 0.000 description 33
- 241000699666 Mus <mouse, genus> Species 0.000 description 31
- 241000700605 Viruses Species 0.000 description 29
- 238000013459 approach Methods 0.000 description 29
- 230000002018 overexpression Effects 0.000 description 29
- SGKRLCUYIXIAHR-AKNGSSGZSA-N (4s,4ar,5s,5ar,6r,12ar)-4-(dimethylamino)-1,5,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4a,5,5a,6-tetrahydro-4h-tetracene-2-carboxamide Chemical compound C1=CC=C2[C@H](C)[C@@H]([C@H](O)[C@@H]3[C@](C(O)=C(C(N)=O)C(=O)[C@H]3N(C)C)(O)C3=O)C3=C(O)C2=C1O SGKRLCUYIXIAHR-AKNGSSGZSA-N 0.000 description 22
- 230000027455 binding Effects 0.000 description 22
- 210000002966 serum Anatomy 0.000 description 18
- 230000001177 retroviral effect Effects 0.000 description 17
- 238000011161 development Methods 0.000 description 16
- 230000018109 developmental process Effects 0.000 description 16
- 230000016784 immunoglobulin production Effects 0.000 description 14
- 201000010099 disease Diseases 0.000 description 12
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 12
- 210000001165 lymph node Anatomy 0.000 description 11
- 239000000203 mixture Substances 0.000 description 11
- 230000028993 immune response Effects 0.000 description 10
- 230000003053 immunization Effects 0.000 description 10
- 238000002649 immunization Methods 0.000 description 10
- 230000002163 immunogen Effects 0.000 description 10
- 238000000338 in vitro Methods 0.000 description 10
- 210000000952 spleen Anatomy 0.000 description 10
- 208000003950 B-cell lymphoma Diseases 0.000 description 9
- 241000699660 Mus musculus Species 0.000 description 9
- 210000001519 tissue Anatomy 0.000 description 9
- 238000011830 transgenic mouse model Methods 0.000 description 9
- 238000009825 accumulation Methods 0.000 description 8
- 150000001413 amino acids Chemical class 0.000 description 8
- 235000005911 diet Nutrition 0.000 description 8
- 230000037213 diet Effects 0.000 description 8
- 238000000684 flow cytometry Methods 0.000 description 8
- 210000003734 kidney Anatomy 0.000 description 8
- 210000001744 T-lymphocyte Anatomy 0.000 description 7
- 230000001419 dependent effect Effects 0.000 description 7
- 230000004044 response Effects 0.000 description 7
- GHASVSINZRGABV-UHFFFAOYSA-N Fluorouracil Chemical compound FC1=CNC(=O)NC1=O GHASVSINZRGABV-UHFFFAOYSA-N 0.000 description 6
- 206010025323 Lymphomas Diseases 0.000 description 6
- 239000002299 complementary DNA Substances 0.000 description 6
- 229960002949 fluorouracil Drugs 0.000 description 6
- 230000001939 inductive effect Effects 0.000 description 6
- 208000015181 infectious disease Diseases 0.000 description 6
- 206010022000 influenza Diseases 0.000 description 6
- 230000007246 mechanism Effects 0.000 description 6
- 230000003472 neutralizing effect Effects 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 241000725303 Human immunodeficiency virus Species 0.000 description 5
- 108700019146 Transgenes Proteins 0.000 description 5
- 238000004458 analytical method Methods 0.000 description 5
- 201000011510 cancer Diseases 0.000 description 5
- 239000012678 infectious agent Substances 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 230000001105 regulatory effect Effects 0.000 description 5
- 230000003612 virological effect Effects 0.000 description 5
- 108020004635 Complementary DNA Proteins 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
- 102000005348 Neuraminidase Human genes 0.000 description 4
- 108010006232 Neuraminidase Proteins 0.000 description 4
- 208000036142 Viral infection Diseases 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 210000002798 bone marrow cell Anatomy 0.000 description 4
- 230000000295 complement effect Effects 0.000 description 4
- 230000004154 complement system Effects 0.000 description 4
- 239000003937 drug carrier Substances 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 108020001507 fusion proteins Proteins 0.000 description 4
- 102000037865 fusion proteins Human genes 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000006166 lysate Substances 0.000 description 4
- 230000001717 pathogenic effect Effects 0.000 description 4
- 230000009257 reactivity Effects 0.000 description 4
- DAEPDZWVDSPTHF-UHFFFAOYSA-M sodium pyruvate Chemical compound [Na+].CC(=O)C([O-])=O DAEPDZWVDSPTHF-UHFFFAOYSA-M 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
- 238000010361 transduction Methods 0.000 description 4
- 230000026683 transduction Effects 0.000 description 4
- 239000013598 vector Substances 0.000 description 4
- 230000009385 viral infection Effects 0.000 description 4
- 238000001262 western blot Methods 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 3
- 101150013553 CD40 gene Proteins 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 3
- 102100038132 Endogenous retrovirus group K member 6 Pro protein Human genes 0.000 description 3
- 108010054477 Immunoglobulin Fab Fragments Proteins 0.000 description 3
- 102000001706 Immunoglobulin Fab Fragments Human genes 0.000 description 3
- 108010067060 Immunoglobulin Variable Region Proteins 0.000 description 3
- 102000017727 Immunoglobulin Variable Region Human genes 0.000 description 3
- 102000004889 Interleukin-6 Human genes 0.000 description 3
- ZDXPYRJPNDTMRX-VKHMYHEASA-N L-glutamine Chemical compound OC(=O)[C@@H](N)CCC(N)=O ZDXPYRJPNDTMRX-VKHMYHEASA-N 0.000 description 3
- 229930182816 L-glutamine Natural products 0.000 description 3
- 208000008771 Lymphadenopathy Diseases 0.000 description 3
- 108091005804 Peptidases Proteins 0.000 description 3
- 239000004365 Protease Substances 0.000 description 3
- 241000607142 Salmonella Species 0.000 description 3
- 206010041660 Splenomegaly Diseases 0.000 description 3
- 102100040245 Tumor necrosis factor receptor superfamily member 5 Human genes 0.000 description 3
- 101150117115 V gene Proteins 0.000 description 3
- 239000012190 activator Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 239000006285 cell suspension Substances 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 238000003776 cleavage reaction Methods 0.000 description 3
- 238000010367 cloning Methods 0.000 description 3
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 210000003743 erythrocyte Anatomy 0.000 description 3
- 239000003797 essential amino acid Substances 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 239000012894 fetal calf serum Substances 0.000 description 3
- 230000035931 haemagglutination Effects 0.000 description 3
- 238000003018 immunoassay Methods 0.000 description 3
- 231100000518 lethal Toxicity 0.000 description 3
- 230000001665 lethal effect Effects 0.000 description 3
- 208000018555 lymphatic system disease Diseases 0.000 description 3
- 210000004962 mammalian cell Anatomy 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 230000003278 mimic effect Effects 0.000 description 3
- 239000012514 monoclonal antibody product Substances 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 230000002093 peripheral effect Effects 0.000 description 3
- 239000002953 phosphate buffered saline Substances 0.000 description 3
- 230000007017 scission Effects 0.000 description 3
- 230000028327 secretion Effects 0.000 description 3
- 230000003393 splenic effect Effects 0.000 description 3
- 230000001225 therapeutic effect Effects 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 241001430294 unidentified retrovirus Species 0.000 description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 241000714266 Bovine leukemia virus Species 0.000 description 2
- 208000011691 Burkitt lymphomas Diseases 0.000 description 2
- 108010047041 Complementarity Determining Regions Proteins 0.000 description 2
- 102100025137 Early activation antigen CD69 Human genes 0.000 description 2
- 208000032163 Emerging Communicable disease Diseases 0.000 description 2
- 241000588722 Escherichia Species 0.000 description 2
- 241000714165 Feline leukemia virus Species 0.000 description 2
- 241000589601 Francisella Species 0.000 description 2
- WZUVPPKBWHMQCE-UHFFFAOYSA-N Haematoxylin Chemical compound C12=CC(O)=C(O)C=C2CC2(O)C1C1=CC=C(O)C(O)=C1OC2 WZUVPPKBWHMQCE-UHFFFAOYSA-N 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 101000934374 Homo sapiens Early activation antigen CD69 Proteins 0.000 description 2
- 108700005091 Immunoglobulin Genes Proteins 0.000 description 2
- 108090000978 Interleukin-4 Proteins 0.000 description 2
- 108090001005 Interleukin-6 Proteins 0.000 description 2
- 101150008942 J gene Proteins 0.000 description 2
- 241000713666 Lentivirus Species 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- 241000186359 Mycobacterium Species 0.000 description 2
- 208000015914 Non-Hodgkin lymphomas Diseases 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 229930182555 Penicillin Natural products 0.000 description 2
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
- 241000224016 Plasmodium Species 0.000 description 2
- 241000288906 Primates Species 0.000 description 2
- 239000012980 RPMI-1640 medium Substances 0.000 description 2
- 241000711798 Rabies lyssavirus Species 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 2
- 241000283984 Rodentia Species 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- 241000191940 Staphylococcus Species 0.000 description 2
- 241000194017 Streptococcus Species 0.000 description 2
- 239000004098 Tetracycline Substances 0.000 description 2
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 2
- 241000607626 Vibrio cholerae Species 0.000 description 2
- 108010003533 Viral Envelope Proteins Proteins 0.000 description 2
- 108010066342 Virus Receptors Proteins 0.000 description 2
- 102000018265 Virus Receptors Human genes 0.000 description 2
- 241000607734 Yersinia <bacteria> Species 0.000 description 2
- 230000001154 acute effect Effects 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 238000010171 animal model Methods 0.000 description 2
- 230000003172 anti-dna Effects 0.000 description 2
- 230000003460 anti-nuclear Effects 0.000 description 2
- 230000006907 apoptotic process Effects 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 150000001720 carbohydrates Chemical class 0.000 description 2
- 235000014633 carbohydrates Nutrition 0.000 description 2
- 230000022131 cell cycle Effects 0.000 description 2
- 239000013592 cell lysate Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000034994 death Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 235000020776 essential amino acid Nutrition 0.000 description 2
- 238000001476 gene delivery Methods 0.000 description 2
- 230000002068 genetic effect Effects 0.000 description 2
- 229940072221 immunoglobulins Drugs 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 238000005304 joining Methods 0.000 description 2
- 210000004698 lymphocyte Anatomy 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 230000036210 malignancy Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000010369 molecular cloning Methods 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 230000009826 neoplastic cell growth Effects 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 210000000056 organ Anatomy 0.000 description 2
- 238000004091 panning Methods 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 229940049954 penicillin Drugs 0.000 description 2
- 239000008194 pharmaceutical composition Substances 0.000 description 2
- 238000003752 polymerase chain reaction Methods 0.000 description 2
- 239000013641 positive control Substances 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 230000002062 proliferating effect Effects 0.000 description 2
- 229940054269 sodium pyruvate Drugs 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 230000005748 tumor development Effects 0.000 description 2
- 241000701161 unidentified adenovirus Species 0.000 description 2
- 229940118696 vibrio cholerae Drugs 0.000 description 2
- 239000013603 viral vector Substances 0.000 description 2
- 125000000349 (Z)-3-carboxyprop-2-enoyl group Chemical group O=C([*])/C([H])=C([H])\C(O[H])=O 0.000 description 1
- -1 2-B mercaptoethanol Chemical compound 0.000 description 1
- 241000712891 Arenavirus Species 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 108091008875 B cell receptors Proteins 0.000 description 1
- 208000004736 B-Cell Leukemia Diseases 0.000 description 1
- 102100022005 B-lymphocyte antigen CD20 Human genes 0.000 description 1
- 241000193830 Bacillus <bacterium> Species 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 241000588807 Bordetella Species 0.000 description 1
- 241000588779 Bordetella bronchiseptica Species 0.000 description 1
- 241000588832 Bordetella pertussis Species 0.000 description 1
- 241000244036 Brugia Species 0.000 description 1
- 241000722910 Burkholderia mallei Species 0.000 description 1
- 241001136175 Burkholderia pseudomallei Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 241000282465 Canis Species 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- 241000606161 Chlamydia Species 0.000 description 1
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 241000224483 Coccidia Species 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 241000711573 Coronaviridae Species 0.000 description 1
- 208000001528 Coronaviridae Infections Diseases 0.000 description 1
- 241000709687 Coxsackievirus Species 0.000 description 1
- 241000699800 Cricetinae Species 0.000 description 1
- 241001337994 Cryptococcus <scale insect> Species 0.000 description 1
- 241000701022 Cytomegalovirus Species 0.000 description 1
- 101150097493 D gene Proteins 0.000 description 1
- 208000005156 Dehydration Diseases 0.000 description 1
- 241000243990 Dirofilaria Species 0.000 description 1
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 1
- 206010013975 Dyspnoeas Diseases 0.000 description 1
- 238000012286 ELISA Assay Methods 0.000 description 1
- 241000588921 Enterobacteriaceae Species 0.000 description 1
- 241000194033 Enterococcus Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 206010015548 Euthanasia Diseases 0.000 description 1
- 241000282324 Felis Species 0.000 description 1
- 241000710831 Flavivirus Species 0.000 description 1
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 1
- 241000589602 Francisella tularensis Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 229930186217 Glycolipid Natural products 0.000 description 1
- 241000941423 Grom virus Species 0.000 description 1
- 241000252870 H3N2 subtype Species 0.000 description 1
- 241001087371 H6N8 subtype Species 0.000 description 1
- 241000252864 H7N2 subtype Species 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 208000031886 HIV Infections Diseases 0.000 description 1
- 108010034145 Helminth Proteins Proteins 0.000 description 1
- 208000002250 Hematologic Neoplasms Diseases 0.000 description 1
- 108010088652 Histocompatibility Antigens Class I Proteins 0.000 description 1
- 102000008949 Histocompatibility Antigens Class I Human genes 0.000 description 1
- 241000228402 Histoplasma Species 0.000 description 1
- 101000897405 Homo sapiens B-lymphocyte antigen CD20 Proteins 0.000 description 1
- 101001033279 Homo sapiens Interleukin-3 Proteins 0.000 description 1
- 101001076408 Homo sapiens Interleukin-6 Proteins 0.000 description 1
- 101000716729 Homo sapiens Kit ligand Proteins 0.000 description 1
- 108090000144 Human Proteins Proteins 0.000 description 1
- 102000003839 Human Proteins Human genes 0.000 description 1
- 241000598436 Human T-cell lymphotropic virus Species 0.000 description 1
- 206010020460 Human T-cell lymphotropic virus type I infection Diseases 0.000 description 1
- 241000714260 Human T-lymphotropic virus 1 Species 0.000 description 1
- 241000714259 Human T-lymphotropic virus 2 Species 0.000 description 1
- 241000701044 Human gammaherpesvirus 4 Species 0.000 description 1
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 1
- 241000713340 Human immunodeficiency virus 2 Species 0.000 description 1
- 206010020631 Hypergammaglobulinaemia benign monoclonal Diseases 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 208000024781 Immune Complex disease Diseases 0.000 description 1
- 206010061598 Immunodeficiency Diseases 0.000 description 1
- 208000029462 Immunodeficiency disease Diseases 0.000 description 1
- 102000018071 Immunoglobulin Fc Fragments Human genes 0.000 description 1
- 108010091135 Immunoglobulin Fc Fragments Proteins 0.000 description 1
- 241000712431 Influenza A virus Species 0.000 description 1
- 208000002979 Influenza in Birds Diseases 0.000 description 1
- 241000222722 Leishmania <genus> Species 0.000 description 1
- 208000030289 Lymphoproliferative disease Diseases 0.000 description 1
- 241000712079 Measles morbillivirus Species 0.000 description 1
- 108010090054 Membrane Glycoproteins Proteins 0.000 description 1
- 102000012750 Membrane Glycoproteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 241000192017 Micrococcaceae Species 0.000 description 1
- 208000034578 Multiple myelomas Diseases 0.000 description 1
- 241000711386 Mumps virus Species 0.000 description 1
- 241000186362 Mycobacterium leprae Species 0.000 description 1
- 241000187479 Mycobacterium tuberculosis Species 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 241000204034 Mycoplasmataceae Species 0.000 description 1
- 108091061960 Naked DNA Proteins 0.000 description 1
- 241000588653 Neisseria Species 0.000 description 1
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 241000713112 Orthobunyavirus Species 0.000 description 1
- 241000702244 Orthoreovirus Species 0.000 description 1
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- 241001631646 Papillomaviridae Species 0.000 description 1
- 241000223785 Paramecium Species 0.000 description 1
- 208000002606 Paramyxoviridae Infections Diseases 0.000 description 1
- 241000606860 Pasteurella Species 0.000 description 1
- 241000606752 Pasteurellaceae Species 0.000 description 1
- 108010033276 Peptide Fragments Proteins 0.000 description 1
- 102000007079 Peptide Fragments Human genes 0.000 description 1
- 201000005702 Pertussis Diseases 0.000 description 1
- 241000223810 Plasmodium vivax Species 0.000 description 1
- 241000233870 Pneumocystis Species 0.000 description 1
- 241000125945 Protoparvovirus Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 1
- 108700008625 Reporter Genes Proteins 0.000 description 1
- 241000725643 Respiratory syncytial virus Species 0.000 description 1
- 241000606701 Rickettsia Species 0.000 description 1
- 241000606683 Rickettsiaceae Species 0.000 description 1
- 241000710799 Rubella virus Species 0.000 description 1
- 230000018199 S phase Effects 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- 206010041052 Sluggishness Diseases 0.000 description 1
- 241000223996 Toxoplasma Species 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 241000700618 Vaccinia virus Species 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 241000607493 Vibrionaceae Species 0.000 description 1
- 230000010530 Virus Neutralization Effects 0.000 description 1
- 208000033559 Waldenström macroglobulinemia Diseases 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000002679 ablation Methods 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 230000004523 agglutinating effect Effects 0.000 description 1
- 238000007818 agglutination assay Methods 0.000 description 1
- RGCKGOZRHPZPFP-UHFFFAOYSA-N alizarin Chemical compound C1=CC=C2C(=O)C3=C(O)C(O)=CC=C3C(=O)C2=C1 RGCKGOZRHPZPFP-UHFFFAOYSA-N 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000005784 autoimmunity Effects 0.000 description 1
- 206010064097 avian influenza Diseases 0.000 description 1
- 229940065181 bacillus anthracis Drugs 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000005422 blasting Methods 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 238000010322 bone marrow transplantation Methods 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 238000010370 cell cloning Methods 0.000 description 1
- 230000007910 cell fusion Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000009137 competitive binding Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000009260 cross reactivity Effects 0.000 description 1
- 230000016396 cytokine production Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000018044 dehydration Effects 0.000 description 1
- 238000006297 dehydration reaction Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000002405 diagnostic procedure Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 206010012818 diffuse large B-cell lymphoma Diseases 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000000539 dimer Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 244000078703 ectoparasite Species 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- YQGOJNYOYNNSMM-UHFFFAOYSA-N eosin Chemical compound [Na+].OC(=O)C1=CC=CC=C1C1=C2C=C(Br)C(=O)C(Br)=C2OC2=C(Br)C(O)=C(Br)C=C21 YQGOJNYOYNNSMM-UHFFFAOYSA-N 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 239000013604 expression vector Substances 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000003325 follicular Effects 0.000 description 1
- 229940118764 francisella tularensis Drugs 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 238000007499 fusion processing Methods 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 244000000013 helminth Species 0.000 description 1
- 208000006454 hepatitis Diseases 0.000 description 1
- 231100000283 hepatitis Toxicity 0.000 description 1
- 208000010726 hind limb paralysis Diseases 0.000 description 1
- 238000010562 histological examination Methods 0.000 description 1
- 102000055276 human IL3 Human genes 0.000 description 1
- 102000055151 human KITLG Human genes 0.000 description 1
- 230000028996 humoral immune response Effects 0.000 description 1
- 230000008348 humoral response Effects 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 208000024326 hypersensitivity reaction type III disease Diseases 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 238000003119 immunoblot Methods 0.000 description 1
- 230000007813 immunodeficiency Effects 0.000 description 1
- 229940099472 immunoglobulin a Drugs 0.000 description 1
- 229940027941 immunoglobulin g Drugs 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000037797 influenza A Diseases 0.000 description 1
- 108700010900 influenza virus proteins Proteins 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000000977 initiatory effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000001990 intravenous administration Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 230000021633 leukocyte mediated immunity Effects 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 210000005210 lymphoid organ Anatomy 0.000 description 1
- 201000000564 macroglobulinemia Diseases 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 210000003519 mature b lymphocyte Anatomy 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 210000004779 membrane envelope Anatomy 0.000 description 1
- 238000000386 microscopy Methods 0.000 description 1
- 201000005328 monoclonal gammopathy of uncertain significance Diseases 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 231100000590 oncogenic Toxicity 0.000 description 1
- 230000002246 oncogenic effect Effects 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- 230000003071 parasitic effect Effects 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 210000005259 peripheral blood Anatomy 0.000 description 1
- 239000011886 peripheral blood Substances 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 201000000317 pneumocystosis Diseases 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 230000003389 potentiating effect Effects 0.000 description 1
- 230000001855 preneoplastic effect Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000000069 prophylactic effect Effects 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000010188 recombinant method Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 238000009877 rendering Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- PYWVYCXTNDRMGF-UHFFFAOYSA-N rhodamine B Chemical compound [Cl-].C=12C=CC(=[N+](CC)CC)C=C2OC2=CC(N(CC)CC)=CC=C2C=1C1=CC=CC=C1C(O)=O PYWVYCXTNDRMGF-UHFFFAOYSA-N 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000010187 selection method Methods 0.000 description 1
- 229940048278 septra Drugs 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 108010061514 sialic acid receptor Proteins 0.000 description 1
- 238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000009870 specific binding Effects 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 230000002269 spontaneous effect Effects 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000012289 standard assay Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 108020001568 subdomains Proteins 0.000 description 1
- JLKIGFTWXXRPMT-UHFFFAOYSA-N sulphamethoxazole Chemical compound O1C(C)=CC(NS(=O)(=O)C=2C=CC(N)=CC=2)=N1 JLKIGFTWXXRPMT-UHFFFAOYSA-N 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 230000002459 sustained effect Effects 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 201000000596 systemic lupus erythematosus Diseases 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
- 229940126622 therapeutic monoclonal antibody Drugs 0.000 description 1
- 239000003104 tissue culture media Substances 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 208000025883 type III hypersensitivity disease Diseases 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 210000000689 upper leg Anatomy 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
- 229940125575 vaccine candidate Drugs 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 210000005253 yeast cell Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
- C07K16/1018—Orthomyxoviridae, e.g. influenza virus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- A61K39/21—Retroviridae, e.g. equine infectious anemia virus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
- C07K14/08—RNA viruses
- C07K14/11—Orthomyxoviridae, e.g. influenza virus
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/08—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses
- C07K16/10—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from viruses from RNA viruses
- C07K16/1036—Retroviridae, e.g. leukemia viruses
- C07K16/1045—Lentiviridae, e.g. HIV, FIV, SIV
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/02—Animal zootechnically ameliorated
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
- C12N2710/16734—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/12011—Betaretrovirus, e.g. mouse mammary tumour virus
- C12N2740/12034—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2740/00—Reverse transcribing RNA viruses
- C12N2740/00011—Details
- C12N2740/10011—Retroviridae
- C12N2740/12011—Betaretrovirus, e.g. mouse mammary tumour virus
- C12N2740/12041—Use of virus, viral particle or viral elements as a vector
- C12N2740/12043—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/16011—Orthomyxoviridae
- C12N2760/16111—Influenzavirus A, i.e. influenza A virus
- C12N2760/16134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Communicable Diseases (AREA)
- Animal Behavior & Ethology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Oncology (AREA)
- Pulmonology (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- AIDS & HIV (AREA)
- Cell Biology (AREA)
- Gastroenterology & Hepatology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US89465407P | 2007-03-13 | 2007-03-13 | |
| US60/894,654 | 2007-03-13 | ||
| US93904207P | 2007-05-18 | 2007-05-18 | |
| US60/939,042 | 2007-05-18 | ||
| PCT/US2008/056896 WO2008112922A2 (en) | 2007-03-13 | 2008-03-13 | Methods for generation of antibodies |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2680613A1 true CA2680613A1 (en) | 2008-09-18 |
Family
ID=39760405
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002680613A Abandoned CA2680613A1 (en) | 2007-03-13 | 2008-03-13 | Methods for generation of antibodies |
Country Status (9)
| Country | Link |
|---|---|
| US (2) | US8551968B2 (de) |
| EP (3) | EP2583550A1 (de) |
| JP (1) | JP2010521170A (de) |
| KR (2) | KR20090130236A (de) |
| CN (1) | CN101688228B (de) |
| AU (1) | AU2008224950B2 (de) |
| CA (1) | CA2680613A1 (de) |
| IL (1) | IL200919A0 (de) |
| WO (1) | WO2008112922A2 (de) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL286053B2 (en) | 2005-10-18 | 2023-03-01 | Nat Jewish Health | A process for making red blood cells using immortal hematopoietic stem cells and erythropoietin |
| US8986702B2 (en) | 2008-05-16 | 2015-03-24 | Taiga Biotechnologies, Inc. | Antibodies and processes for preparing the same |
| SG193145A1 (en) | 2008-07-21 | 2013-09-30 | Taiga Biotechnologies Inc | Differentiated anucleated cells and method for preparing the same |
| EP2318435B1 (de) | 2008-08-28 | 2015-12-23 | Taiga Biotechnologies, Inc. | Myc-modulatoren, verfahren zu ihrer verwendung und verfahren zur identifikation von mitteln zur myc-modulierung |
| EP2449094A4 (de) * | 2009-06-29 | 2013-07-10 | Qingfeng Chen | Verfahren zur herstellung humanisierter nichtmenschlicher säugetiere |
| US20120021409A1 (en) * | 2010-02-08 | 2012-01-26 | Regeneron Pharmaceuticals, Inc. | Common Light Chain Mouse |
| US20130045492A1 (en) | 2010-02-08 | 2013-02-21 | Regeneron Pharmaceuticals, Inc. | Methods For Making Fully Human Bispecific Antibodies Using A Common Light Chain |
| US9796788B2 (en) | 2010-02-08 | 2017-10-24 | Regeneron Pharmaceuticals, Inc. | Mice expressing a limited immunoglobulin light chain repertoire |
| HUE026229T2 (en) | 2010-02-08 | 2016-06-28 | Regeneron Pharma | Common light chain mouse |
| EP2739740B1 (de) | 2011-08-05 | 2019-10-02 | Regeneron Pharmaceuticals, Inc. | Universelle, leichtkettige, humanisierte mäuse |
| MY173376A (en) | 2012-03-16 | 2020-01-21 | Regeneron Pharma | Mice that produce antigen?binding proteins with ph?dependent binding characteristics |
| US20140013456A1 (en) | 2012-03-16 | 2014-01-09 | Regeneron Pharmaceuticals, Inc. | Histidine Engineered Light Chain Antibodies and Genetically Modified Non-Human Animals for Generating the Same |
| MX355944B (es) | 2012-03-16 | 2018-05-07 | Regeneron Pharma | Animales no humanos que expresan secuencias de inmunoglobulina sensibles al ph. |
| KR102228296B1 (ko) | 2012-03-16 | 2021-03-17 | 리제너론 파마슈티칼스 인코포레이티드 | 히스티딘 공학처리된 경쇄 항체 및 그것을 생성하기 위한 유전자 변형된 비-사람 동물 |
| CA3133302A1 (en) | 2012-07-20 | 2014-01-23 | Taiga Biotechnologies, Inc. | Enhanced reconstitution and autoreconstitution of the hematopoietic compartment comprising a myc polypeptide |
| US9365825B2 (en) | 2013-03-11 | 2016-06-14 | Taiga Biotechnologies, Inc. | Expansion of adult stem cells in vitro |
| US10272115B2 (en) | 2013-03-11 | 2019-04-30 | Taiga Biotechnologies, Inc. | Production and use of red blood cells |
| EP3119194B1 (de) | 2014-03-21 | 2021-04-28 | Regeneron Pharmaceuticals, Inc. | Einzeldomänenbindende proteine produzierende nichtmenschliche tiere |
| AU2016232715A1 (en) | 2015-03-19 | 2017-09-28 | Regeneron Pharmaceuticals, Inc. | Non-human animals that select for light chain variable regions that bind antigen |
| EP4063862A1 (de) | 2015-10-21 | 2022-09-28 | Redcoat Solutions, Inc. | Bettwanzenerfassungsvorrichtung |
| MD3365367T2 (ro) | 2015-10-21 | 2024-02-29 | Redcoat Solutions Inc | Anticorpi monoclonali împotriva ploşniţelor de pat și procedee de obţinere și utilizare a acestora |
| CN106566843B (zh) * | 2016-11-09 | 2019-06-07 | 中国人民解放军第四五八医院 | 一种BALB/c背景的年龄依赖的乙型肝炎小鼠模型系统的构建方法及其应用 |
| AU2017367730A1 (en) | 2016-12-02 | 2019-06-06 | Taiga Biotechnologies, Inc. | Nanoparticle formulations |
| US10149898B2 (en) | 2017-08-03 | 2018-12-11 | Taiga Biotechnologies, Inc. | Methods and compositions for the treatment of melanoma |
| KR20210133234A (ko) | 2019-02-18 | 2021-11-05 | 바이오사이토젠 파마슈티컬스 (베이징) 컴퍼니 리미티드 | 인간화 면역글로불린 유전자좌를 갖는 유전적으로 변형된 비-인간 동물 |
| IL298632A (en) | 2020-06-02 | 2023-01-01 | Biocytogen Pharmaceuticals Beijing Co Ltd | Non-human animals genetically modified with a common light chain immunoglobulin locus |
Family Cites Families (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5476996A (en) | 1988-06-14 | 1995-12-19 | Lidak Pharmaceuticals | Human immune system in non-human animal |
| US5849288A (en) | 1990-01-15 | 1998-12-15 | Yeda Research And Development Co. Ltd. | Method for production of monoclonal antibodies in chimeric mice or rats having xenogeneic antibody-producing cells |
| US6255458B1 (en) * | 1990-08-29 | 2001-07-03 | Genpharm International | High affinity human antibodies and human antibodies against digoxin |
| ES2123062T3 (es) | 1992-08-21 | 1999-01-01 | Biogen Inc | Polipeptidos de transporte derivados de la proteina tat. |
| US5580760A (en) | 1993-02-22 | 1996-12-03 | The United States Of America As Represented By The Department Of Health And Human Services | FUSE binding protein and cDNA therefor |
| EP0710283A4 (de) * | 1993-07-22 | 1997-07-02 | Merck & Co Inc | DIE EXPRESSION VON MENSCHLICHEM INTERLEUKIN-1-g(b) IN EINEM TRANSGENEM TIER |
| US5599705A (en) | 1993-11-16 | 1997-02-04 | Cameron; Robert B. | In vitro method for producing differentiated universally compatible mature human blood cells |
| DE19541844C1 (de) * | 1995-11-09 | 1997-07-24 | Gsf Forschungszentrum Umwelt | Verfahren zur Herstellung von menschlichen Antikörpern und deren Verwendung |
| US6713247B1 (en) | 1996-09-03 | 2004-03-30 | Signal Pharmaceuticials, Inc. | Human CNS cell lines and methods of use therefor |
| US6835567B1 (en) | 1998-04-14 | 2004-12-28 | Signal Pharmaceuticals, Inc. | PNS cell lines and methods of use therefor |
| US6913925B1 (en) | 1998-08-12 | 2005-07-05 | Signal Pharmaceuticals Llc | Human mesencephalon cell lines and methods of use therefor |
| WO2000062067A1 (en) | 1999-02-28 | 2000-10-19 | Washington University | Novel transduction molecules and methods for using same |
| US7135287B1 (en) | 1999-10-02 | 2006-11-14 | Biosite, Inc. | Human antibodies |
| US7311920B1 (en) | 1999-10-08 | 2007-12-25 | University Of Maryland Biotechnology Institute | Virus coat protein/receptor chimeras and methods of use |
| EP1103615A1 (de) | 1999-11-25 | 2001-05-30 | Universite De Geneve | Vektoren, die nicht-teilende Zellen immortalisieren können und Zellen, die mit diesen Vektoren immortalisiert wurden |
| US20010049393A1 (en) | 1999-12-07 | 2001-12-06 | Whitehead Institute For Biomedical Research | Methods for defining MYC target genes and uses thereof |
| WO2001087058A1 (fr) | 2000-05-15 | 2001-11-22 | Sonoko Habu | Souris chimerique ayant une immunite creee par utilisation de cellules positives cd34 humaines et utilisation de cette souris |
| US20030072794A1 (en) | 2000-06-09 | 2003-04-17 | Teni Boulikas | Encapsulation of plasmid DNA (lipogenes™) and therapeutic agents with nuclear localization signal/fusogenic peptide conjugates into targeted liposome complexes |
| US20070248618A1 (en) | 2004-03-16 | 2007-10-25 | Cohen David I | Tat-Based vaccine Compositions and Methods of Making and Using Same |
| US7145055B2 (en) | 2000-12-01 | 2006-12-05 | Central Institute For Experimental Animals | Method of producing a mouse suitable for the engraftment, differentiation and proliferation of heterologous cells, mouse produced by this method and use of the mouse |
| GB0117631D0 (en) * | 2001-07-19 | 2001-09-12 | Syngenta Ltd | Improvements in or relating to organic compounds |
| CN1240439C (zh) | 2002-03-28 | 2006-02-08 | 南京凯基生物科技发展有限公司 | 肿瘤基因开关药物 |
| GB2387599B (en) * | 2002-04-17 | 2005-08-10 | Jason Peter Brown | Methods for producing antibodies |
| DK1408114T3 (da) | 2002-10-11 | 2007-05-07 | Imvision Gmbh | Modulære antigen-transporter molekyler (MAT-molekyler) til modulation af immunreaktioner, tilhörende konstrukter, fremgangsmåder og anvendelser |
| WO2004035535A2 (en) | 2002-10-15 | 2004-04-29 | Yeda Research And Development Co. Ltd. | Erythrocyte differentiation factor, gene encoding same, and methods of use thereof |
| WO2005014785A2 (en) | 2003-06-18 | 2005-02-17 | The George Washington University | Conditionally-immortalized hematopoietic progenitor cell lines |
| US7705049B2 (en) | 2004-01-21 | 2010-04-27 | New York University | Methods for treating non-melanoma cancers with PABA |
| GB0420963D0 (en) * | 2004-09-21 | 2004-10-20 | Reneuron Ltd | Hepatocyte |
| WO2006045064A2 (en) | 2004-10-20 | 2006-04-27 | Whitehead Institute For Biomedical Research | Cultured hematopoietic stem cells and method for expansion and analysis thereof |
| US8795650B2 (en) | 2004-12-09 | 2014-08-05 | The Regents Of The University Of California | Derivation of unlimited quantities of neutrophils or monocyte/dendritic cells |
| US20060156422A1 (en) | 2005-01-10 | 2006-07-13 | Medical Research Council | Methods and compositions for the generation of antibodies |
| WO2006116512A1 (en) | 2005-04-26 | 2006-11-02 | The Board Of Trustees Of The University Of Illinois, Urbana, Il | Nucleoside compounds and methods of use thereof |
| US20070093420A1 (en) | 2005-08-26 | 2007-04-26 | Yeomans David C | Therapy procedure for drug delivery for trigeminal pain |
| IL286053B2 (en) | 2005-10-18 | 2023-03-01 | Nat Jewish Health | A process for making red blood cells using immortal hematopoietic stem cells and erythropoietin |
| AU2006311725B2 (en) | 2005-11-04 | 2011-11-24 | Alnylam Pharmaceuticals, Inc. | Compositions and methods for inhibiting expression of NAV1.8 gene |
| EP1792627A1 (de) | 2005-12-05 | 2007-06-06 | ImVisioN AG | Eine Intralymphatische Transduktion Allergen (ITAG) enthaltende Zusammensetzung zur Verwendung in der Modulation des Immunsystems |
| US8986702B2 (en) | 2008-05-16 | 2015-03-24 | Taiga Biotechnologies, Inc. | Antibodies and processes for preparing the same |
| SG193145A1 (en) | 2008-07-21 | 2013-09-30 | Taiga Biotechnologies Inc | Differentiated anucleated cells and method for preparing the same |
| EP2318435B1 (de) | 2008-08-28 | 2015-12-23 | Taiga Biotechnologies, Inc. | Myc-modulatoren, verfahren zu ihrer verwendung und verfahren zur identifikation von mitteln zur myc-modulierung |
-
2008
- 2008-03-13 EP EP12187077.8A patent/EP2583550A1/de not_active Withdrawn
- 2008-03-13 KR KR1020097021320A patent/KR20090130236A/ko not_active Application Discontinuation
- 2008-03-13 CN CN200880015602.7A patent/CN101688228B/zh not_active Expired - Fee Related
- 2008-03-13 CA CA002680613A patent/CA2680613A1/en not_active Abandoned
- 2008-03-13 EP EP08743862A patent/EP2134852A4/de not_active Withdrawn
- 2008-03-13 KR KR1020137020078A patent/KR20140092232A/ko not_active Application Discontinuation
- 2008-03-13 EP EP12187097.6A patent/EP2583551A1/de not_active Withdrawn
- 2008-03-13 WO PCT/US2008/056896 patent/WO2008112922A2/en active Application Filing
- 2008-03-13 US US12/048,148 patent/US8551968B2/en active Active
- 2008-03-13 AU AU2008224950A patent/AU2008224950B2/en not_active Ceased
- 2008-03-13 JP JP2009553785A patent/JP2010521170A/ja active Pending
-
2009
- 2009-09-14 IL IL200919A patent/IL200919A0/en unknown
-
2013
- 2013-09-04 US US14/018,366 patent/US20140162357A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CN101688228B (zh) | 2014-12-24 |
| AU2008224950B2 (en) | 2013-10-03 |
| WO2008112922A2 (en) | 2008-09-18 |
| US20140162357A1 (en) | 2014-06-12 |
| KR20140092232A (ko) | 2014-07-23 |
| IL200919A0 (en) | 2011-07-31 |
| EP2134852A2 (de) | 2009-12-23 |
| KR20090130236A (ko) | 2009-12-21 |
| EP2583551A1 (de) | 2013-04-24 |
| EP2583550A1 (de) | 2013-04-24 |
| AU2008224950A1 (en) | 2008-09-18 |
| US8551968B2 (en) | 2013-10-08 |
| EP2134852A4 (de) | 2010-04-28 |
| CN101688228A (zh) | 2010-03-31 |
| WO2008112922A3 (en) | 2008-11-06 |
| JP2010521170A (ja) | 2010-06-24 |
| US20100279351A1 (en) | 2010-11-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8551968B2 (en) | Methods for generation of antibodies | |
| Zinkernagel et al. | Neutralizing antiviral antibody responses | |
| Gupta et al. | DNA vaccine molecular adjuvants SP-D-BAFF and SP-D-APRIL enhance anti-gp120 immune response and increase HIV-1 neutralizing antibody titers | |
| DeFranco | The germinal center antibody response in health and disease | |
| US10233424B2 (en) | Compositions and methods including cytotoxic B lymphocyte cell line expressing exogenous membrane immunoglobulin different from secreted immunoglobulin | |
| US20110008332A1 (en) | Combination Therapy to Treat Persistent Viral Infections | |
| JP2023528017A (ja) | 重症急性呼吸器症候群コロナウイルス２（ｓａｒｓ－ｃｏｖ－２）ポリペプチドおよびワクチン目的でのその使用 | |
| US20160046907A1 (en) | In vitro system for generation of antigen-specific immune responses | |
| EP2892561A1 (de) | Hiv-impfstoffzusammensetzungen und verfahren | |
| US8926995B2 (en) | Adjuvant | |
| AU2013273777A1 (en) | Methods for generation of antibodies | |
| WO2022101302A1 (en) | Antibodies conjugated or fused to the receptor-binding domain of the sars-cov-2 spike protein and uses thereof for vaccine purposes | |
| Naz et al. | Immunotherapeutic and immunomodulatory potentials of antigen-antibody complex vaccines | |
| Mitra-Kaushik et al. | Idiotypic–anti-idiotypic B cell interactions generated against a protective antigen of a morbillivirus in mice | |
| Ertl et al. | Modulation of the immune response by anti-idiotypic antibodies with specificity for B-or T-cell idiotypes | |
| WO2023088968A1 (en) | Universal sarbecovirus vaccines | |
| US20220048988A1 (en) | Cells expressing antibodies targeting human immunodeficiency virus and methods of using the same | |
| JP5996437B2 (ja) | 抗原特異的モノクローナル抗体産生ハイブリドーマの作製方法及び抗原特異的モノクローナル抗体の製造方法 | |
| Gary | Enhancing the Immunogenicity of Anti-HIV DNA Vaccines with Molecular Adjuvants | |
| KALINKE et al. | Neutralizing Antiviral Antibody Responses | |
| Yang | Immunity and Autoimmunity: Host Mimicry by HIV-1 | |
| Deak | The role of the lymph node and B cells in visceral Leishmania pathogenesis | |
| US20100303858A1 (en) | Chimeric HIV-1 Glycoproteins and Their Biological Applications | |
| Maris et al. | CD137 induction of tolerogenic dendritic cells during | |
| KR20100058141A (ko) | 수지상 세포의 에프씨 수용체를 표적화한 말 헤르페스 바이러스 백신 제제 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20130307 |
|
| FZDE | Discontinued |
Effective date: 20160530 |