CA2660171C - Production of high tryptophan maize by chloroplast targeted expression of anthranilate synthase - Google Patents

Production of high tryptophan maize by chloroplast targeted expression of anthranilate synthase Download PDF

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Publication number
CA2660171C
CA2660171C CA2660171A CA2660171A CA2660171C CA 2660171 C CA2660171 C CA 2660171C CA 2660171 A CA2660171 A CA 2660171A CA 2660171 A CA2660171 A CA 2660171A CA 2660171 C CA2660171 C CA 2660171C
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CA
Canada
Prior art keywords
ctp
seq
anthranilate synthase
plant cell
gfp
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Active
Application number
CA2660171A
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English (en)
French (fr)
Other versions
CA2660171A1 (en
Inventor
Siva Manjunath
Santiago Xavier Navarro
William D. Rapp
Xiaohong Shi
Marguerite J. Varagona
Jennifer L. Winson
Guangning Ye
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Monsanto Technology LLC
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Monsanto Technology LLC
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Publication date
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Publication of CA2660171A1 publication Critical patent/CA2660171A1/en
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Publication of CA2660171C publication Critical patent/CA2660171C/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8251—Amino acid content, e.g. synthetic storage proteins, altering amino acid biosynthesis
    • C12N15/8254—Tryptophan or lysine

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
CA2660171A 2006-08-11 2007-08-09 Production of high tryptophan maize by chloroplast targeted expression of anthranilate synthase Active CA2660171C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US83720006P 2006-08-11 2006-08-11
US60/837,200 2006-08-11
PCT/US2007/075614 WO2008021974A1 (en) 2006-08-11 2007-08-09 Production of high tryptophan maize by chloroplast targeted expression of anthranilate synthase

Publications (2)

Publication Number Publication Date
CA2660171A1 CA2660171A1 (en) 2008-02-21
CA2660171C true CA2660171C (en) 2017-05-16

Family

ID=38860117

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2660171A Active CA2660171C (en) 2006-08-11 2007-08-09 Production of high tryptophan maize by chloroplast targeted expression of anthranilate synthase

Country Status (11)

Country Link
US (2) US8138393B2 (ja)
EP (1) EP2049670A1 (ja)
JP (1) JP5296684B2 (ja)
KR (1) KR101447300B1 (ja)
CN (1) CN101522902B (ja)
AU (1) AU2007286176B2 (ja)
BR (1) BRPI0716657B1 (ja)
CA (1) CA2660171C (ja)
MX (1) MX2009001573A (ja)
NZ (1) NZ574568A (ja)
WO (1) WO2008021974A1 (ja)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5296684B2 (ja) 2006-08-11 2013-09-25 モンサント テクノロジー エルエルシー アントラニル酸合成酵素の葉緑体標的発現による高トリプトファントウモロコシの生産
US20090205075A1 (en) * 2008-01-30 2009-08-13 Stacy Miles Use of plastid transit peptides derived from glaucocystophytes
KR20110014607A (ko) 2008-04-29 2011-02-11 아보트 러보러터리즈 이원 가변 도메인 면역글로불린 및 이의 용도
MX2010013239A (es) 2008-06-03 2011-02-24 Abbott Lab Inmunoglobulinas de dominio variable doble y usos de las mismas.
UY33492A (es) 2010-07-09 2012-01-31 Abbott Lab Inmunoglobulinas con dominio variable dual y usos de las mismas
TW201206473A (en) 2010-08-03 2012-02-16 Abbott Lab Dual variable domain immunoglobulins and uses thereof
AU2011289283B2 (en) 2010-08-13 2015-04-30 Pioneer Hi-Bred International, Inc. Chimeric promoters and methods of use
US9150625B2 (en) 2011-05-23 2015-10-06 E I Du Pont De Nemours And Company Chloroplast transit peptides and methods of their use
CA2844101A1 (en) 2011-08-03 2013-02-07 E. I. Du Pont De Nemours And Company Methods and compositions for targeted integration in a plant
CA2850390A1 (en) 2011-10-28 2013-05-02 E. I. Du Pont De Nemours And Company Methods and compositions for silencing genes using artificial micrornas
AR089793A1 (es) 2012-01-27 2014-09-17 Du Pont Metodos y composiciones para generar locus de rasgos transgenicos complejos
CN108192901A (zh) * 2012-02-01 2018-06-22 陶氏益农公司 人造叶绿体转运肽
US9663793B2 (en) 2012-04-20 2017-05-30 Monsanto Technology, Llc Plant regulatory elements and uses thereof
BR112016003591A8 (pt) 2013-08-22 2018-01-30 Du Pont promotor u6 de polimerase iii de soja e métodos de uso
WO2016007347A1 (en) 2014-07-11 2016-01-14 E. I. Du Pont De Nemours And Company Compositions and methods for producing plants resistant to glyphosate herbicide
EP3191595B1 (en) 2014-09-12 2019-12-25 E. I. du Pont de Nemours and Company Generation of site-specific-integration sites for complex trait loci in corn and soybean, and methods of use
CN107429258A (zh) 2015-03-19 2017-12-01 先锋国际良种公司 用于加速的性状渐渗的方法和组合物
US20180258438A1 (en) 2015-11-06 2018-09-13 Pioneer Hi-Bred International, Inc. Generation of complex trait loci in soybean and methods of use
US20200407737A1 (en) 2017-05-03 2020-12-31 KWS SAAT SE & Co. KGaA Use of crispr-cas endonucleases for plant genome engineering
US11761012B2 (en) 2017-11-03 2023-09-19 University Of Florida Research Foundation, Incorporated Mitigation of maize heat stress with recombinant 6-phosphogluconate dehydrogenase
CN108531645B (zh) * 2018-07-09 2021-07-27 江苏省农业科学院 低直链淀粉含量基因wx-C39的功能标记及其应用
US11041172B2 (en) 2019-06-25 2021-06-22 Inari Agriculture, Inc. Homology dependent repair genome editing

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE93542T1 (de) 1984-12-28 1993-09-15 Plant Genetic Systems Nv Rekombinante dna, die in pflanzliche zellen eingebracht werden kann.
AU590597B2 (en) 1985-08-07 1989-11-09 Monsanto Technology Llc Glyphosate-resistant plants
US7705215B1 (en) 1990-04-17 2010-04-27 Dekalb Genetics Corporation Methods and compositions for the production of stably transformed, fertile monocot plants and cells thereof
DK0546090T4 (da) * 1990-08-31 2006-11-20 Monsanto Co Glyphosattolerante enolpyruvylshikimat-3-phosphat-synthaser
US5633435A (en) * 1990-08-31 1997-05-27 Monsanto Company Glyphosate-tolerant 5-enolpyruvylshikimate-3-phosphate synthases
US5545545A (en) * 1993-04-27 1996-08-13 Regents Of The University Of Minnesota Lysine-insensitive maize dihydrodipicolinic acid synthase
US6118047A (en) 1993-08-25 2000-09-12 Dekalb Genetic Corporation Anthranilate synthase gene and method of use thereof for conferring tryptophan overproduction
US6571016B1 (en) * 1997-05-05 2003-05-27 Microsoft Corporation Intra compression of pixel blocks using predicted mean
CN1719971B (zh) 2001-05-04 2011-04-13 雷尼森有限责任公司 转基因高色氨酸植物细胞
US7714189B2 (en) 2002-05-03 2010-05-11 Monsanto Technology Llc High tryptophan maize
CA2482879C (en) * 2002-05-03 2013-02-12 Monsanto Technology, Llc Transgenic high tryptophan plants
JP5296684B2 (ja) 2006-08-11 2013-09-25 モンサント テクノロジー エルエルシー アントラニル酸合成酵素の葉緑体標的発現による高トリプトファントウモロコシの生産

Also Published As

Publication number Publication date
KR101447300B1 (ko) 2014-10-07
US20080050506A1 (en) 2008-02-28
BRPI0716657A2 (pt) 2013-12-03
US20120246762A1 (en) 2012-09-27
JP2010500035A (ja) 2010-01-07
AU2007286176A1 (en) 2008-02-21
KR20090058519A (ko) 2009-06-09
WO2008021974A1 (en) 2008-02-21
MX2009001573A (es) 2009-02-19
NZ574568A (en) 2011-04-29
BRPI0716657B1 (pt) 2018-04-17
JP5296684B2 (ja) 2013-09-25
EP2049670A1 (en) 2009-04-22
US8138393B2 (en) 2012-03-20
US8981188B2 (en) 2015-03-17
CN101522902B (zh) 2015-08-26
AU2007286176B2 (en) 2012-10-11
CA2660171A1 (en) 2008-02-21
CN101522902A (zh) 2009-09-02

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