CA2642514C - Synthesis of error-minimized nucleic acid molecules - Google Patents

Synthesis of error-minimized nucleic acid molecules Download PDF

Info

Publication number
CA2642514C
CA2642514C CA2642514A CA2642514A CA2642514C CA 2642514 C CA2642514 C CA 2642514C CA 2642514 A CA2642514 A CA 2642514A CA 2642514 A CA2642514 A CA 2642514A CA 2642514 C CA2642514 C CA 2642514C
Authority
CA
Canada
Prior art keywords
molecules
nucleotide sequence
fragments
sequence
intended
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CA2642514A
Other languages
English (en)
French (fr)
Other versions
CA2642514A1 (en
Inventor
Lei Young
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Viridos Inc
Original Assignee
Synthetic Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38092910&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CA2642514(C) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Synthetic Genomics Inc filed Critical Synthetic Genomics Inc
Publication of CA2642514A1 publication Critical patent/CA2642514A1/en
Application granted granted Critical
Publication of CA2642514C publication Critical patent/CA2642514C/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CA2642514A 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules Active CA2642514C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US74146905P 2005-12-02 2005-12-02
US60/741,469 2005-12-02
PCT/US2006/046457 WO2007065035A2 (en) 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules

Publications (2)

Publication Number Publication Date
CA2642514A1 CA2642514A1 (en) 2007-06-07
CA2642514C true CA2642514C (en) 2011-06-07

Family

ID=38092910

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2642514A Active CA2642514C (en) 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules

Country Status (6)

Country Link
US (1) US7704690B2 (enExample)
EP (1) EP1963525B2 (enExample)
JP (1) JP4789271B2 (enExample)
AU (1) AU2006320275B2 (enExample)
CA (1) CA2642514C (enExample)
WO (1) WO2007065035A2 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1915446T3 (en) 2005-08-11 2017-09-11 Synthetic Genomics Inc IN VITRO RECOMBINATION PROCEDURE
US20100323404A1 (en) * 2007-02-09 2010-12-23 Richard Lathrop Method for recombining dna sequences and compositions related thereto
EP3064599B1 (en) * 2008-02-15 2018-12-12 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
US10093552B2 (en) 2008-02-22 2018-10-09 James Weifu Lee Photovoltaic panel-interfaced solar-greenhouse distillation systems
US9259662B2 (en) 2008-02-22 2016-02-16 James Weifu Lee Photovoltaic panel-interfaced solar-greenhouse distillation systems
US8986963B2 (en) 2008-02-23 2015-03-24 James Weifu Lee Designer calvin-cycle-channeled production of butanol and related higher alcohols
US8735651B2 (en) 2008-02-23 2014-05-27 James Weifu Lee Designer organisms for photobiological butanol production from carbon dioxide and water
EP2664678B1 (en) * 2008-10-24 2014-10-08 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
CN102858995B (zh) 2009-09-10 2016-10-26 森特瑞隆技术控股公司 靶向测序方法
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US12595500B2 (en) 2011-09-26 2026-04-07 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
LT2768607T (lt) 2011-09-26 2021-12-27 Thermo Fisher Scientific Geneart Gmbh Daugiašulininė plokštelė didelio efektyvumo, mažo tūrio nukleorūgščių sintezei atlikti
WO2014106167A1 (en) * 2012-12-31 2014-07-03 Advanced Liquid Logic, Inc. Digital microfluidic gene synthesis and error correction
US20160017394A1 (en) 2014-07-15 2016-01-21 Life Technologies Corporation Compositions and methods for nucleic acid assembly
WO2016094512A1 (en) 2014-12-09 2016-06-16 yLIFE TECHNOLOGIES CORPORATION High efficiency, small volume nucleic acid synthesis
US20180195099A1 (en) 2015-07-07 2018-07-12 Thermo Fisher Scientific Geneart Gmbh Enzymatic synthesis of nucleic acid sequences
EP3763818A1 (en) 2015-10-06 2021-01-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
US12595476B2 (en) * 2018-03-19 2026-04-07 Modernatx, Inc. Assembly and error reduction of synthetic genes from oligonucleotides
US20210147830A1 (en) 2018-06-29 2021-05-20 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
EP4114972A1 (en) 2020-03-06 2023-01-11 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
WO2024132094A1 (en) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Retrieval of sequence-verified nucleic acid molecules

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20040023327A1 (en) * 1995-12-07 2004-02-05 Short Jay M. End selection in directed evolution
CN100422323C (zh) * 2000-04-07 2008-10-01 荣研化学株式会社 使用双链核酸为模板扩增核酸的方法
AU2002230526A1 (en) * 2000-12-01 2002-06-11 Cornell Research Foundation, Inc. Detection of nucleic acid differences using combined endonuclease cleavage and ligation reactions
US7879580B2 (en) * 2002-12-10 2011-02-01 Massachusetts Institute Of Technology Methods for high fidelity production of long nucleic acid molecules
US20050106590A1 (en) * 2003-05-22 2005-05-19 Lathrop Richard H. Method for producing a synthetic gene or other DNA sequence
CA2578564C (en) * 2004-08-27 2013-05-28 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US20070196834A1 (en) * 2005-09-09 2007-08-23 Francesco Cerrina Method and system for the generation of large double stranded DNA fragments

Also Published As

Publication number Publication date
WO2007065035A2 (en) 2007-06-07
EP1963525B1 (en) 2013-11-20
EP1963525A4 (en) 2009-03-18
EP1963525B2 (en) 2017-10-11
US7704690B2 (en) 2010-04-27
WO2007065035A3 (en) 2007-11-22
CA2642514A1 (en) 2007-06-07
AU2006320275A1 (en) 2007-06-07
AU2006320275B2 (en) 2012-06-07
US20070128649A1 (en) 2007-06-07
JP4789271B2 (ja) 2011-10-12
EP1963525A2 (en) 2008-09-03
JP2009518013A (ja) 2009-05-07

Similar Documents

Publication Publication Date Title
AU2006320275B2 (en) Synthesis of error-minimized nucleic acid molecules
CN106062209B (zh) 合成性长阅读dna测序
US9255291B2 (en) Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing
EP2944693B1 (en) Compositions and methods for high fidelity assembly of nucleic acids
AU2005279931B2 (en) Method of error reduction in nucleic acid populations
JP2009518013A5 (enExample)
JP2007534303A (ja) 合成遺伝子または他のｄｎａ配列を作製するための方法
CA3178211A1 (en) Methods for ligation-coupled-pcr
EP1394251A1 (en) Method for the selective randomization of polynucleotides
CN102325898A (zh) 基于pcr的合成核酸分子的方法
WO2017183648A1 (ja) 多項目増幅手法
JP7677978B2 (ja) 遺伝子変異ライブラリーを構築する方法
WO2025172578A1 (en) A nucleic acid assembly method using type iib enzymes
WO2025054499A1 (en) Compositions and methods relating to enzymatic dna synthesis
GB2644434A (en) Junction Regions, Templates and Methods for Nucleic Acid Synthesis
JP2025083598A (ja) Ｒｎａ／ｄｎａキメラプライマーを用いたｐｃｒ及びその増幅断片連結方法
CN119213122A (zh) sgRNA测序接头及其应用
Quan Genetic Assembly, Error-Correction and a High-Throughput Screening Strategy for
HK1221710A1 (zh) 一种基於分子反向探针的测序文库构建方法和试剂
HK1230669A1 (en) Synthetic long read dna sequencing
KR20160149158A (ko) 고집적도 올리고뉴클레오티드 디자인을 통한 유전자 합성 방법

Legal Events

Date Code Title Description
EEER Examination request
MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 18TH ANNIV.) - STANDARD

Year of fee payment: 18

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20241202

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT DETERMINED COMPLIANT

Effective date: 20241202

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20241202

MPN Maintenance fee for patent paid

Free format text: FEE DESCRIPTION TEXT: MF (PATENT, 19TH ANNIV.) - STANDARD

Year of fee payment: 19

U00 Fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED

Effective date: 20251201

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL

Effective date: 20251201