AU2006320275B2 - Synthesis of error-minimized nucleic acid molecules - Google Patents

Synthesis of error-minimized nucleic acid molecules Download PDF

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Publication number
AU2006320275B2
AU2006320275B2 AU2006320275A AU2006320275A AU2006320275B2 AU 2006320275 B2 AU2006320275 B2 AU 2006320275B2 AU 2006320275 A AU2006320275 A AU 2006320275A AU 2006320275 A AU2006320275 A AU 2006320275A AU 2006320275 B2 AU2006320275 B2 AU 2006320275B2
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molecules
nucleotide sequence
fragments
sequence
intended
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AU2006320275A1 (en
Inventor
Lei Young
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Viridos Inc
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Synthetic Genomics Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AU2006320275A 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules Active AU2006320275B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US74146905P 2005-12-02 2005-12-02
US60/741,469 2005-12-02
PCT/US2006/046457 WO2007065035A2 (en) 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules

Publications (2)

Publication Number Publication Date
AU2006320275A1 AU2006320275A1 (en) 2007-06-07
AU2006320275B2 true AU2006320275B2 (en) 2012-06-07

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AU2006320275A Active AU2006320275B2 (en) 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules

Country Status (6)

Country Link
US (1) US7704690B2 (enExample)
EP (1) EP1963525B2 (enExample)
JP (1) JP4789271B2 (enExample)
AU (1) AU2006320275B2 (enExample)
CA (1) CA2642514C (enExample)
WO (1) WO2007065035A2 (enExample)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1915446T3 (en) * 2005-08-11 2017-09-11 Synthetic Genomics Inc IN VITRO RECOMBINATION PROCEDURE
US20100323404A1 (en) * 2007-02-09 2010-12-23 Richard Lathrop Method for recombining dna sequences and compositions related thereto
DK2255013T3 (en) * 2008-02-15 2016-09-12 Synthetic Genomics Inc Methods for in vitro joining and combinatorial assembly of nucleic acid molecules.
US9259662B2 (en) 2008-02-22 2016-02-16 James Weifu Lee Photovoltaic panel-interfaced solar-greenhouse distillation systems
US10093552B2 (en) 2008-02-22 2018-10-09 James Weifu Lee Photovoltaic panel-interfaced solar-greenhouse distillation systems
US8986963B2 (en) * 2008-02-23 2015-03-24 James Weifu Lee Designer calvin-cycle-channeled production of butanol and related higher alcohols
AU2009217293B2 (en) 2008-02-23 2014-11-20 James Weifu Lee Designer organisms for photobiological butanol production from carbon dioxide and water
DK2376517T3 (da) * 2008-10-24 2013-02-11 Epict Technologies Corp Transposon-ende-sammensætninger og fremgangsmåder til at modificere nukleinsyrer
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US10072287B2 (en) 2009-09-10 2018-09-11 Centrillion Technology Holdings Corporation Methods of targeted sequencing
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US20120252682A1 (en) * 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
US20130109596A1 (en) 2011-09-26 2013-05-02 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2014106167A1 (en) * 2012-12-31 2014-07-03 Advanced Liquid Logic, Inc. Digital microfluidic gene synthesis and error correction
US20160017394A1 (en) 2014-07-15 2016-01-21 Life Technologies Corporation Compositions and methods for nucleic acid assembly
CA2970477C (en) 2014-12-09 2022-03-15 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
WO2017007925A1 (en) 2015-07-07 2017-01-12 Thermo Fisher Scientific Geneart Gmbh Enzymatic synthesis of nucleic acid sequences
US20180291413A1 (en) 2015-10-06 2018-10-11 Thermo Fisher Scientific Geneart Gmbh Devices and methods for producing nucleic acids and proteins
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
WO2019183055A1 (en) * 2018-03-19 2019-09-26 Modernatx, Inc. Assembly and error reduction of synthetic genes from oligonucleotides
WO2020001783A1 (en) 2018-06-29 2020-01-02 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
JP2023516827A (ja) 2020-03-06 2023-04-20 ライフ テクノロジーズ コーポレイション 高配列忠実度の核酸合成およびアセンブリ
WO2024132094A1 (en) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Retrieval of sequence-verified nucleic acid molecules

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050255477A1 (en) * 2002-12-10 2005-11-17 Carr Peter A Methods for high fidelity production of long nucleic acid molecules

Family Cites Families (8)

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Publication number Priority date Publication date Assignee Title
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20040023327A1 (en) * 1995-12-07 2004-02-05 Short Jay M. End selection in directed evolution
EP2253717B1 (en) * 2000-04-07 2014-04-30 Eiken Kagaku Kabushiki Kaisha Method of amplifying nucleic acid by using double-stranded nucleic acid as template
AU2002230526A1 (en) * 2000-12-01 2002-06-11 Cornell Research Foundation, Inc. Detection of nucleic acid differences using combined endonuclease cleavage and ligation reactions
US20050106590A1 (en) * 2003-05-22 2005-05-19 Lathrop Richard H. Method for producing a synthetic gene or other DNA sequence
EP1781786B1 (en) * 2004-08-27 2011-01-26 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US20070196834A1 (en) * 2005-09-09 2007-08-23 Francesco Cerrina Method and system for the generation of large double stranded DNA fragments

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050255477A1 (en) * 2002-12-10 2005-11-17 Carr Peter A Methods for high fidelity production of long nucleic acid molecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YOUNG, L. et al., Nucleic Acids Research, 2004, vol. 32, e59 pages 1-6 *

Also Published As

Publication number Publication date
JP2009518013A (ja) 2009-05-07
EP1963525B1 (en) 2013-11-20
EP1963525A2 (en) 2008-09-03
WO2007065035A2 (en) 2007-06-07
EP1963525A4 (en) 2009-03-18
EP1963525B2 (en) 2017-10-11
CA2642514C (en) 2011-06-07
AU2006320275A1 (en) 2007-06-07
US20070128649A1 (en) 2007-06-07
CA2642514A1 (en) 2007-06-07
WO2007065035A3 (en) 2007-11-22
JP4789271B2 (ja) 2011-10-12
US7704690B2 (en) 2010-04-27

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