AU2006320275B2 - Synthesis of error-minimized nucleic acid molecules - Google Patents

Synthesis of error-minimized nucleic acid molecules Download PDF

Info

Publication number
AU2006320275B2
AU2006320275B2 AU2006320275A AU2006320275A AU2006320275B2 AU 2006320275 B2 AU2006320275 B2 AU 2006320275B2 AU 2006320275 A AU2006320275 A AU 2006320275A AU 2006320275 A AU2006320275 A AU 2006320275A AU 2006320275 B2 AU2006320275 B2 AU 2006320275B2
Authority
AU
Australia
Prior art keywords
molecules
nucleotide sequence
fragments
sequence
intended
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2006320275A
Other languages
English (en)
Other versions
AU2006320275A1 (en
Inventor
Lei Young
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Viridos Inc
Original Assignee
Synthetic Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=38092910&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=AU2006320275(B2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Synthetic Genomics Inc filed Critical Synthetic Genomics Inc
Publication of AU2006320275A1 publication Critical patent/AU2006320275A1/en
Application granted granted Critical
Publication of AU2006320275B2 publication Critical patent/AU2006320275B2/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1027—Mutagenizing nucleic acids by DNA shuffling, e.g. RSR, STEP, RPR

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
AU2006320275A 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules Active AU2006320275B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US74146905P 2005-12-02 2005-12-02
US60/741,469 2005-12-02
PCT/US2006/046457 WO2007065035A2 (en) 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules

Publications (2)

Publication Number Publication Date
AU2006320275A1 AU2006320275A1 (en) 2007-06-07
AU2006320275B2 true AU2006320275B2 (en) 2012-06-07

Family

ID=38092910

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2006320275A Active AU2006320275B2 (en) 2005-12-02 2006-12-04 Synthesis of error-minimized nucleic acid molecules

Country Status (6)

Country Link
US (1) US7704690B2 (enExample)
EP (1) EP1963525B2 (enExample)
JP (1) JP4789271B2 (enExample)
AU (1) AU2006320275B2 (enExample)
CA (1) CA2642514C (enExample)
WO (1) WO2007065035A2 (enExample)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK1915446T3 (en) 2005-08-11 2017-09-11 Synthetic Genomics Inc IN VITRO RECOMBINATION PROCEDURE
US20100323404A1 (en) * 2007-02-09 2010-12-23 Richard Lathrop Method for recombining dna sequences and compositions related thereto
EP3064599B1 (en) * 2008-02-15 2018-12-12 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
US10093552B2 (en) 2008-02-22 2018-10-09 James Weifu Lee Photovoltaic panel-interfaced solar-greenhouse distillation systems
US9259662B2 (en) 2008-02-22 2016-02-16 James Weifu Lee Photovoltaic panel-interfaced solar-greenhouse distillation systems
US8986963B2 (en) 2008-02-23 2015-03-24 James Weifu Lee Designer calvin-cycle-channeled production of butanol and related higher alcohols
US8735651B2 (en) 2008-02-23 2014-05-27 James Weifu Lee Designer organisms for photobiological butanol production from carbon dioxide and water
EP2664678B1 (en) * 2008-10-24 2014-10-08 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
US9080211B2 (en) * 2008-10-24 2015-07-14 Epicentre Technologies Corporation Transposon end compositions and methods for modifying nucleic acids
CN102858995B (zh) 2009-09-10 2016-10-26 森特瑞隆技术控股公司 靶向测序方法
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
WO2014153188A2 (en) 2013-03-14 2014-09-25 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
US12595500B2 (en) 2011-09-26 2026-04-07 Life Technologies Corporation High efficiency, small volume nucleic acid synthesis
LT2768607T (lt) 2011-09-26 2021-12-27 Thermo Fisher Scientific Geneart Gmbh Daugiašulininė plokštelė didelio efektyvumo, mažo tūrio nukleorūgščių sintezei atlikti
WO2014106167A1 (en) * 2012-12-31 2014-07-03 Advanced Liquid Logic, Inc. Digital microfluidic gene synthesis and error correction
US20160017394A1 (en) 2014-07-15 2016-01-21 Life Technologies Corporation Compositions and methods for nucleic acid assembly
WO2016094512A1 (en) 2014-12-09 2016-06-16 yLIFE TECHNOLOGIES CORPORATION High efficiency, small volume nucleic acid synthesis
US20180195099A1 (en) 2015-07-07 2018-07-12 Thermo Fisher Scientific Geneart Gmbh Enzymatic synthesis of nucleic acid sequences
EP3763818A1 (en) 2015-10-06 2021-01-13 Pierce Biotechnology, Inc. Devices and methods for producing proteins
GB2566986A (en) 2017-09-29 2019-04-03 Evonetix Ltd Error detection during hybridisation of target double-stranded nucleic acid
US12595476B2 (en) * 2018-03-19 2026-04-07 Modernatx, Inc. Assembly and error reduction of synthetic genes from oligonucleotides
US20210147830A1 (en) 2018-06-29 2021-05-20 Thermo Fisher Scientific Geneart Gmbh High throughput assembly of nucleic acid molecules
GB201905303D0 (en) 2019-04-15 2019-05-29 Thermo Fisher Scient Geneart Gmbh Multiplex assembly of nucleic acid molecules
EP4114972A1 (en) 2020-03-06 2023-01-11 Life Technologies Corporation High sequence fidelity nucleic acid synthesis and assembly
WO2024132094A1 (en) 2022-12-19 2024-06-27 Thermo Fisher Scientific Geneart Gmbh Retrieval of sequence-verified nucleic acid molecules

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050255477A1 (en) * 2002-12-10 2005-11-17 Carr Peter A Methods for high fidelity production of long nucleic acid molecules

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5605793A (en) * 1994-02-17 1997-02-25 Affymax Technologies N.V. Methods for in vitro recombination
US6165793A (en) * 1996-03-25 2000-12-26 Maxygen, Inc. Methods for generating polynucleotides having desired characteristics by iterative selection and recombination
US20040023327A1 (en) * 1995-12-07 2004-02-05 Short Jay M. End selection in directed evolution
CN100422323C (zh) * 2000-04-07 2008-10-01 荣研化学株式会社 使用双链核酸为模板扩增核酸的方法
AU2002230526A1 (en) * 2000-12-01 2002-06-11 Cornell Research Foundation, Inc. Detection of nucleic acid differences using combined endonuclease cleavage and ligation reactions
US20050106590A1 (en) * 2003-05-22 2005-05-19 Lathrop Richard H. Method for producing a synthetic gene or other DNA sequence
CA2578564C (en) * 2004-08-27 2013-05-28 Wisconsin Alumni Research Foundation Method of error reduction in nucleic acid populations
US20070196834A1 (en) * 2005-09-09 2007-08-23 Francesco Cerrina Method and system for the generation of large double stranded DNA fragments

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050255477A1 (en) * 2002-12-10 2005-11-17 Carr Peter A Methods for high fidelity production of long nucleic acid molecules

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YOUNG, L. et al., Nucleic Acids Research, 2004, vol. 32, e59 pages 1-6 *

Also Published As

Publication number Publication date
WO2007065035A2 (en) 2007-06-07
EP1963525B1 (en) 2013-11-20
EP1963525A4 (en) 2009-03-18
EP1963525B2 (en) 2017-10-11
US7704690B2 (en) 2010-04-27
WO2007065035A3 (en) 2007-11-22
CA2642514A1 (en) 2007-06-07
AU2006320275A1 (en) 2007-06-07
US20070128649A1 (en) 2007-06-07
JP4789271B2 (ja) 2011-10-12
EP1963525A2 (en) 2008-09-03
JP2009518013A (ja) 2009-05-07
CA2642514C (en) 2011-06-07

Similar Documents

Publication Publication Date Title
CA2642514C (en) Synthesis of error-minimized nucleic acid molecules
CN106062209B (zh) 合成性长阅读dna测序
US9255291B2 (en) Oligonucleotide ligation methods for improving data quality and throughput using massively parallel sequencing
CN105861487B (zh) 用于靶向核酸序列富集和高效文库产生的组合物和方法
AU2005279931B2 (en) Method of error reduction in nucleic acid populations
JP2007534303A (ja) 合成遺伝子または他のｄｎａ配列を作製するための方法
JP2009518013A5 (enExample)
JP6876785B2 (ja) 単分子配列決定のための一本鎖環状ｄｎａライブラリーを生成するための方法
CA3178211A1 (en) Methods for ligation-coupled-pcr
JP2015500012A (ja) 組成物中のｒｎａを特徴づける方法およびキット
EP1394251A1 (en) Method for the selective randomization of polynucleotides
EP2152901B1 (en) Method for introducing common and/or individual sequence elements in a target nucleic acid molecule
WO2010071602A1 (en) Pcr-based method of synthesizing a nucleic acid molecule
WO2017183648A1 (ja) 多項目増幅手法
JP7677978B2 (ja) 遺伝子変異ライブラリーを構築する方法
CN112941147A (zh) 一种高保真靶标基因建库方法及其试剂盒
WO2015089339A2 (en) Compositions, methods and kits for dna fragmentation and tagmentation
CA2962254A1 (en) Composition and method for processing dna
CN114616342B (zh) 制备用于crispr的个性化的靶标不相关的向导rna池的方法
WO2025172578A1 (en) A nucleic acid assembly method using type iib enzymes
GB2644434A (en) Junction Regions, Templates and Methods for Nucleic Acid Synthesis
WO2025054499A1 (en) Compositions and methods relating to enzymatic dna synthesis
CN113755545A (zh) 一种痕量单链核酸样本制备方法
Quan Genetic Assembly, Error-Correction and a High-Throughput Screening Strategy for
HK1221710A1 (zh) 一种基於分子反向探针的测序文库构建方法和试剂

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)